Neural Stem Cells and Neurospheres - Re-evaluating the Relationship

For most of the past century, the prospect of replacing lost or damaged cells in the central nervous system (CNS) was hampered by the opinion that the adult mammalian CNS was incapable of generating new nerve cells. This belief, Like most dogmas, was essentially founded on a lack of experimental evidence to the contrary. The overturning of this 'no new neuron' hypothesis began midway through the twentieth century with a series of reports documenting neurogenesis in the postnatal and adult brain(1), continued with the isolation and in vitro culture of neurogenic cells from the adult mammalian brain(2,3), and culminated in the discovery of a population of muttipotent, selfrenewing cells in the adult CNS (that is, bona fide neural stem cells)(3-5). Although a variety of techniques were initially used, the neurosphere assay (NSA)(3,6) rapidly emerged as the assay of choice and has since become a valuable toot for isolating, and understanding the biology of, embryonic and adult CNS stem cells. Like all technologies, it is not without its limitations. In this article we will hightight several shortcomings of the assay related to its application and interpretation that we believe have led to a significant body of research whose conclusions may well be misleading.

Synthesis, biological activity, and preliminary pharmacokinetic evaluation of analogues of a phosphosulfomannan angiogenesis inhibitor (PI-88)

The phosphosulfomannan 1 (PI-88) is a mixture of highly sulfated oligosaccharides that is currently undergoing clinical evaluation in cancer patients. As well as it's anticancer properties, 1 displays a number of other interesting biological activities. A series of analogues of 1 were synthesized with a single carbon (pentasaccharide) backbone to facilitate structural characterization and interpretation of biological results. In a fashion similar to 1, all compounds were able to inhibit heparanase and to bind tightly to the proangiogenic growth factors FGF-1, FGF-2, and VEGF. The compounds also inhibited the infection of cells and cell-to-cell spread of herpes simplex virus (HSV-1). Preliminary pharmacokinetic data indicated that the compounds displayed different pharmacokinetic behavior compared with 1. Of particular note was the n-octyl derivative, which was cleared 3 times less rapidly than 1 and may provide increased systemic exposure.

An archaeology of the instant? Action and narrative in microscopic archaeological residue analyses

The discovery and interpretation of microscopic residues on stone artefacts is an expanding front within archaeological science, allowing reconstructions of the past use of specific tools. With notable exceptions, however, the field has seen little theoretical development, relying largely on a rationale in which either individual findings are widely generalized or the age of the site determines the importance of the results. Here an approach to residue interpretation is proposed that draws on notions of narrative, scale, action and agency as one means of expanding the theoretical scope and application of residue studies. It is suggested that the individual resonance of the findings of residue analyses with people in the present day can be used to provide a more nuanced understanding of past actions, which in turn allows both better integration and communication of those findings within and outside the archaeological comm unity, and begins to overcome the problems associated with the typically small sample sizes analysed in stone-tool residue studies.

Tempest in another time: Shakespeare, Greenaway, Céline

Hainge examines Shakespeare's play The Tempest, which was used in the allusions of Louis-Ferdinand Céline's novel Guignol's Band and Peter Greenaway's 1991 film Prospero's Book. He hypothesizes that Céline's novel Feerie pour une autre fois can be conceived of as a response to and interpretation of the The Tempest in the same way that Greenaway's film can be conceived of not as a representation of Shakespeare's play, a simple staging of it, but rather as a subjective response to it, an adaptation in the truest sense of the term.

The cultural facet of suicidal behaviour: Its importance and neglect

Culture is a central but debated concept in many disciplines and its complexity may become an even bigger source of argument in Suicidology. In spite of the intricacy of the study of this construct, the paper illustrates that various scholars have recognised the relevance of culture and ethnicity in the understanding of suicidal behaviour. The author provides evidence of the need to pay more attention to the meaning and interpretation of suicide in cross-cultural research and underlines the necessity to establish cultural-sensitive prevention strategies. The paper closes by providing methodological considerations and suggestions for future research on cultural aspects of suicidal behaviour.

Signal or Noise? Information Theory and the Novel

In the context of a hostile funding environment, universities are increasingly asked to justify their output in narrowly defined economic terms, and this can be difficult in Humanities or Arts faculties where productivity is rarely reducible to a simple financial indicator. This can lead to a number of immediate consequences that I have no need to rehearse here, but can also result in some interesting tensions within the academic community itself. First is that which has become known as the ‘Science Wars’: the increasingly acrimonious exchanges between scientists and scientific academics and cultural critics or theorists about who has the right to describe the world. Much has already been said—and much remains to be said—about this issue, but it is not my intention to discuss it here. Rather, I will look at a second area of contestation: the incorporation of scientific theory into literary or cultural criticism. Much of this work comes from a genuine commitment to interdisciplinarity, and an appreciation of insights that a fresh perspective can bring to a familiar object. However, some can be seen as cynical attempts to lend literary studies the sort of empirical legitimacy of the sciences. In particular, I want to look at a number of critics who have applied information theory to the literary work. In this paper, I will examine several instances of this sort of criticism, and then, through an analysis of a novel by American author Richard Powers, Three Farmers on Their Way to a Dance, show how this sort of criticism merely reduces the meaningful analysis of a complex literary text.

MIQUÉIAS 6,1-8: UM TEXTO PARADIGMÁTICO NA INTERFACE DA CRÍTICA PROFÉTICA COM A SABEDORIA ISRAELITA

A presente dissertação de mestrado em Literatura e Religião no Mundo Bíblico tem por objetivo realizar um comentário exegético e hermenêutico de um texto reconhecido como profético e sua relação no plano teológico, antropológico e literário com o universo sapiencial israelita no período pós-exílico. Trata-se do estudo de Miquéias 6,1-8, cujo foco de investigação desenvolveu-se a partir da análise do discurso e da hipótese de confluência de gêneros literários, a saber, o profético e o sapiencial. Considerado sob os aspectos formais, contextuais e de conteúdo antropo teológico, o texto estudado apresenta-se como resultado da composição de diversos gêneros literários e manifesta, internamente, conflitos de teologias que vão desde a interpretação da própria história de Israel até a prática religiosa com suas concepções de Deus. Miquéias 6,1-8, interpretado aqui a partir de metodologias exegéticas modernas e abordagens contextuais e antropológicas, configura-se como uma verdadeira síntese de interpretação deuteronomista não hegemônica dos eventos do êxodo e da mensagem dos profetas bíblicos do século VIII aeC Miquéias, Amós, Oséias e Isaías. Estamos diante de um texto que se apresenta, ao mesmo tempo, coeso e portador de diferentes universos e vozes em sua composição. Seu discurso, cujo teor nasce do conflito entre projetos e grupos no período pós-exílico, resgata memórias antigas de um êxodo que passa por sujeitos marginais e reinterpreta a crítica profética em sua função de discernimento ético e teológico, porém, no formato sapiencial. Pela profundidade sócio teológica e pela proposta não sacrificial de seu discurso, Miquéias 6,1-8 tem sido um texto continuamente revisitado no interior da Teologia da Libertação na América Latina, inspirando boa parte de sua produção.

Cristianismo Arreligioso: uma análise do desenvolvimento e recepção da teologia de Dietrich Bonhoeffer

Esta dissertação é o resultado da pesquisa sobre a teologia de Dietrich Bonhoeffer a partir de seus próprios escritos e da recepção e interpretação de seu pensamento por teólogos que se dedicaram ao estudo de sua reflexão teológica após sua morte, especialmente no que diz respeito à sua proposta de um cristianismo arreligioso. Dentre estes teólogos destacam-se Eberhard Bethge, autor de uma detalhada biografia de Bonhoeffer, além de Robinson e Hamilton, autores que participam do movimento que ficou conhecido como Teologia Radical . O objetivo da pesquisa é exatamente esclarecer o significado da proposta de um cristianismo arreligioso em Bonhoeffer, bem como analisar suas críticas à religião e as possibilidades encontradas em sua compreensão cristológica para uma vivência cristã autêntica num mundo que se tornou adulto. (AU)

O DEBATE SOBRE A HISTÓRIA DAS ORIGENS DO TRABALHO BATISTA NO BRASIL Uma análise das relações e dos conflitos de gênero e poder na Convenção Batista Brasileira dos anos 1960-1980

O presente trabalho analisou as relações e os conflitos de gênero e poder observados durante o debate sobre as origens do trabalho batista no Brasil, debate esse entre o Pastor José dos Reis Pereira, líder oficial da Convenção Batista Brasileira durante os anos 1960-1980 e a pesquisadora batista Betty Antunes de Oliveira. A análise do conflito foi realizada principalmente com a mediação de gênero como instrumento hermenêutico, conforme os pressupostos de Joan Wallach Scott. Desse modo, a pesquisa teve como propósito principal, a partir da análise do debate, dar visibilidade ao conflito de gênero nos lugares de poder da Convenção Batista Brasileira dos anos 1960-1980, conflito dissimulado pelos discursos batistas sobre direitos de liberdade e igualdade sociais. Esta pesquisa trabalhou basicamente com as seguintes hipóteses: a dinâmica do debate foi fortalecida pelo contexto sociopolítico daqueles anos, que favoreceu a emergência dos movimentos de mulheres e feminista no Brasil, cujas influências foram também sentidas em outras tradições de fé cristã; e o resultado final do debate dependeu mais das questões de gênero e poder do que das discussões técnicas e acadêmicas sobre o acerto histórico do marco inicial do trabalho batista no Brasil. O ineditismo desta pesquisa está em oferecer uma nova perspectiva do debate sobre as origens do trabalho batista no Brasil, a partir do uso da categoria de gênero como instrumento de análise, o que complementará, desse modo, a pesquisa acadêmica já publicada sobre o tema.

Practices and views on fetal heart monitoring:A structured observation and interview study

Objective: To assess and explain deviations from recommended practice in National Institute for Clinical Excellence (NICE) guidelines in relation to fetal heart monitoring. Design: Qualitative study. Setting: Large teaching hospital in the UK. Sample: Sixty-six hours of observation of 25 labours and interviews with 20 midwives of varying grades. Methods: Structured observations of labour and semistructured interviews with midwives. Interviews were undertaken using a prompt guide, audiotaped, and transcribed verbatim. Analysis was based on the constant comparative method, assisted by QSR N5 software. Main outcome measures: Deviations from recommended practice in relation to fetal monitoring and insights into why these occur. Results: All babies involved in the study were safely delivered, but 243 deviations from recommended practice in relation to NICE guidelines on fetal monitoring were identified, with the majority (80%) of these occurring in relation to documentation. Other deviations from recommended practice included indications for use of electronic fetal heart monitoring and conduct of fetal heart monitoring. There is evidence of difficulties with availability and maintenance of equipment, and some deficits in staff knowledge and skill. Differing orientations towards fetal monitoring were reported by midwives, which were likely to have impacts on practice. The initiation, management, and interpretation of fetal heart monitoring is complex and distributed across time, space, and professional boundaries, and practices in relation to fetal heart monitoring need to be understood within an organisational and social context. Conclusion: Some deviations from best practice guidelines may be rectified through straightforward interventions including improved systems for managing equipment and training. Other deviations from recommended practice need to be understood as the outcomes of complex processes that are likely to defy easy resolution. © RCOG 2006.

Estimation of functional connectivity from electromagnetic signals and the amount of empirical data required

An increasing number of neuroimaging studies are concerned with the identification of interactions or statistical dependencies between brain areas. Dependencies between the activities of different brain regions can be quantified with functional connectivity measures such as the cross-correlation coefficient. An important factor limiting the accuracy of such measures is the amount of empirical data available. For event-related protocols, the amount of data also affects the temporal resolution of the analysis. We use analytical expressions to calculate the amount of empirical data needed to establish whether a certain level of dependency is significant when the time series are autocorrelated, as is the case for biological signals. These analytical results are then contrasted with estimates from simulations based on real data recorded with magnetoencephalography during a resting-state paradigm and during the presentation of visual stimuli. Results indicate that, for broadband signals, 50-100 s of data is required to detect a true underlying cross-correlations coefficient of 0.05. This corresponds to a resolution of a few hundred milliseconds for typical event-related recordings. The required time window increases for narrow band signals as frequency decreases. For instance, approximately 3 times as much data is necessary for signals in the alpha band. Important implications can be derived for the design and interpretation of experiments to characterize weak interactions, which are potentially important for brain processing.

The discretionary role of the line manager in inspiring work engagement and innovative behaviour:a study of social exchange and job resources in the public sector

This thesis is about the discretionary role of the line manager in inspiring the work engagement of staff and their resulting innovative behaviour examined through the lens of Social Exchange Theory (Blau, 1964) and the Job Demands-Resources theory (Bakker, Demerouti, Nachreiner & Schaufeli, 2001). The study is focused on a large British Public Sector organisation undergoing a major organisational shift in the way in which they operate as part of the public sector. It is often claimed that people do not leave organisations; they leave line managers (Kozlowski & Doherty, 1989). Regardless of the knowledge in the literature concerning the importance of the line manager in organisations (Purcell, 2003), the engagement literature in particular is lacking in the consideration of such a fundamental figure in organisational life. Further, the understanding of the black box of managerial discretion and its relationship to employee and organisation related outcomes would benefit from greater exploration (Purcell, 2003; Gerhart, 2005; Scott, et al, 2009). The purpose of this research is to address these gaps with relation to the innovative behaviour of employees in the public sector – an area that is not typically associated with the public sector (Bhatta, 2003; McGuire, Stoner & Mylona, 2008; Hughes, Moore & Kataria, 2011). The study is a CASE Award PhD thesis, requiring academic and practical elements to the research. The study is of one case organisation, focusing on one service characterised by a high level of adoption of Strategic Human Resource Management activities and operating in a rather unique manner for the public sector, having private sector competition for work. The study involved a mixed methods approach to data collection. Preliminary focus groups with 45 participants were conducted, followed by an ethnographic period of five months embedded into the service conducting interviews and observations. This culminated in a quantitative survey delivered within the wider directorate to approximately 500 staff members. The study used aspects of the Grounded Theory (Glaser & Strauss, 1967) approach to analyse the data and developed results that highlight the importance of the line manager in an area characterised by SHRM and organisational change for engaging employees and encouraging innovative behaviour. This survey was completed on behalf of the organisation and the findings of this are presented in appendix 1, in order to keep the focus of the PhD on theory development. Implications for theory and practice are discussed alongside the core finding. Line managers’ discretion surrounding the provision of job resources (in particular trust, autonomy and implementation and interpretation of combined bundles of SHRM policies and procedures) influenced the exchange process by which employees responded with work engagement and innovative behaviour. Limitations to the research are the limitations commonly attributed to cross-sectional data collection methods and those surrounding generalisability of the qualitative findings outside of the contextual factors characterising the service area. Suggestions for future research involve addressing these limitations and further exploration of the discretionary role with regards to extending our understanding of line manager discretion.

Reflecting on time, timing and timeliness in public sector supply networks

Despite recent research on time (e.g. Hedaa & Törnroos, 2001), consideration of the time dimension in data collection, analysis and interpretation in research in supply networks is, to date, still limited. Drawing on a body of literature from organization studies, and empirical findings from a six-year action research programme and a related study of network learning, we reflect on time, timing and timeliness in interorganizational networks. The empirical setting is supply networks in the English health sector wherein we identify and elaborate various issues of time, within the case and in terms of research process. Our analysis is wide-ranging and multi-level, from the global (e.g. identifying the notion of life cycles) to the particular (e.g. different cycle times in supply, such as daily for deliveries and yearly for contracts). We discuss the ‘speeding up’ of inter-organizational ‘e’ time and tensions with other time demands. In closing the paper, we relate our conclusions to the future conduct of the research programme and supply research more generally, and to the practice of managing supply (in) networks.

From art for arts sake to art as means of knowing:a rationale for advancing arts-based methods in research, practice and pedagogy

This paper advances a philosophically informed rationale for the broader, reflexive and practical application of arts-based methods to benefit research, practice and pedagogy. It addresses the complexity and diversity of learning and knowing, foregrounding a cohabitative position and recognition of a plurality of research approaches, tailored and responsive to context. Appreciation of art and aesthetic experience is situated in the everyday, underpinned by multi-layered exemplars of pragmatic visual-arts narrative inquiry undertaken in the third, creative and communications sectors. Discussion considers semi-guided use of arts-based methods as a conduit for topic engagement, reflection and intersubjective agreement; alongside observation and interpretation of organically employed approaches used by participants within daily norms. Techniques span handcrafted (drawing), digital (photography), hybrid (cartooning), performance dimensions (improvised installations) and music (metaphor and structure). The process of creation, the artefact/outcome produced and experiences of consummation are all significant, with specific reflexivity impacts. Exploring methodology and epistemology, both the "doing" and its interpretation are explicated to inform method selection, replication, utility, evaluation and development of cross-media skills literacy. Approaches are found engaging, accessible and empowering, with nuanced capabilities to alter relationships with phenomena, experiences and people. By building a discursive space that reduces barriers; emancipation, interaction, polyphony, letting-go and the progressive unfolding of thoughts are supported, benefiting ways of knowing, narrative (re)construction, sensory perception and capacities to act. This can also present underexplored researcher risks in respect to emotion work, self-disclosure, identity and agenda. The paper therefore elucidates complex, intricate relationships between form and content, the represented and the representation or performance, researcher and participant, and the self and other. This benefits understanding of phenomena including personal experience, sensitive issues, empowerment, identity, transition and liminality. Observations are relevant to qualitative and mixed methods researchers and a multidisciplinary audience, with explicit identification of challenges, opportunities and implications.