994 resultados para 148-896A


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分别制备经1 Gy C离子辐照和未经辐照人肝癌HepG2细胞的总蛋白样品,采用固相pH梯度双向凝胶电泳技术进行蛋白分离,用ImageMaster 2D双向电泳凝胶图像分析软件分析数字化图谱。结果显示,差异表达的蛋白质点为17个,其中1个仅在未经C离子辐照的HepG2细胞中表达,8个蛋白质点在辐照后的细胞中表达上调,8个蛋白质点在辐照后的细胞中表达下调。建立了重复性较好和分辨率较高的分离HepG2细胞总蛋白的双向电泳技术。实验结果表明,C离子辐照HepG2细胞后其蛋白质组发生了改变。

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当归新品系DGA2000-02是采用重离子束55MeV/u40Ar+15离子辐照甘肃当归90-01干种子,按新品种选育程序多年选育而成的。2005—2007年,在定西市岷县、渭源县、漳县、陇西县等地当归品系区域试验中,甘肃当归DGA2000-02平均产鲜当归10621.5kg/hm2,较对照品种(甘肃当归90-01)平均增产鲜当归1386.0kg/hm2,增产率15.0%。生育期790d,茎秆深紫色,根系黄白色。测定结果:总灰分4.2%,酸不溶性灰分0.4%,分别优于对照品种16%和33.3%;浸出物61.4%,较规定指标提高4.4%;阿魏酸0.148%,较规定指标提高2.96倍。质量显著优于对照品种和2005年版《中国药典》规定指标。适宜在海拔2000—2600m、年降水量500—600mm的二阴及高寒阴湿生态区栽培。

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首先介绍了小波变换的基本原理,并在此基础上说明了如何根据所要分析的信号设计双正交小波。实践结果表明,将基于特定信号设计的双正交小波变换技术应用到图像处理上会有很好的效果。

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IEECAS SKLLQG

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利用能量为 16 4— 180MeV的 3 5Cl束流 ,通过重离子核反应 14 9Sm(3 5Cl ,5n)研究了 179Au的高自旋态能级结构 .实验进行了γ射线的激发函数、X γ和γ γ t符合测量 .基于对实验测量结果的分析 ,首次建立了 179Au的 1 2 [6 6 0 ](πi13 2 )转动带 .结合已有的实验数据 ,着重讨论了奇AAu核中1 2 [6 6 0 ](πi13 2 )转动带的带头激发能随中子数的变化

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研究了基于双核模型的粒子交换势能面.原子核的形变效应对于势能面的形状有较大的影响.在反应过程中,作为反应时间函数的动态形变的变化是显著的.通过求解主方程,对一些基于冷熔合机制的反应道的全熔合几率也进行了讨论.

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IEECAS SKLLQG

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IEECAS SKLLQG

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Charge state distribution of 0.8MeV/u uranium ions after transmission through a thin carbon foil has been studied. It is observed that the charge state distribution is equilibrated after the uranium ions have passed through a 15 mu g/cm(2) carbon foil. The equilibrated average charge state is 33.72 and the charge equilibration time of uranium ions in carbon foil is less than 5.4fs.

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The aim of this study was to evaluate the protective effects of different doses and administration modes of N-acetylcysteine (NAC) against X-ray-induced liver damage in mice. Kun-Ming mice were divided into four groups, each composed of six animals: two control groups and two NAC-treated groups. An acute study was carried out to determine alterations in lipid peroxidation (determined by measuring malondiadehyde (MDA) level), glutathione (GSH) content and superoxide dismutase (SOD) activity (assayed by colorimetric method), and DNA damage (characterized by DNA-single strand break using with comet assay) as well as cell apoptosis (measured by flow cytometry) at 12 h after irradiation. The results showed that there were dose-related decreases in MDA level, DNA damage and cell apoptosis, and dose-dependent increases in GSH content and SOD activity in all NAC-treated groups compared to control groups, indicating that pre-treatment or post-treatment with NAC significantly attenuates the acute liver damage caused by X-ray. In addition, significant positive correlations were observed between MDA level and DNA damage or cell apoptosis, implying that lipid peroxidation plays a major role in X-ray-induced liver injury. The data suggest that NAC exerts its radioprotective effect by counteracting accumulated reactive oxygen species in the liver through its properties as a direct antioxidant and a GSH precursor, when administered before or after X-ray irradiation.

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The proton-rich isotope Ho-148 was produced via the fusion-evaporation reaction Mo-92 (Ni-58, 3p1n). The beta-delayed proton decay of Ho-146 was studied by proton-gamma coincidence measurements using a He-jet tape transport system. The gamma-transitions in Tb-145 following the proton emissions were observed, and the beta-delayed proton branching ratios to the final states in the grand-daughter nucleus Tb-145 were determined. According to the relative branching ratios, the ground-state spin of Ho-146 has been proposed and the possible configuration discussed.

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将Frankia菌株CcOl与金色链霉菌GL原生质体融合,得到3株融合子,均具有GL生长快的特性与CcOl的结瘤固氮能力.固体培养时,3株融合子呈现出与GL不同的颜色;且均具有Frankia菌的顶囊形态,以及链霉菌的链状孢子丝结构.与两亲本相同,3株融合子均对大肠杆菌有抗性,其中F4与GL的抗菌谱基本相同.在传10代之后,它们仍具有结瘤与固氮能力.血清学分析表明,F1与F6兼具两亲本的特异抗原,而F4仅具有GL的特异抗原,融合子F1、F6较F4在遗传上更为稳定.

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2001~2003年在长白山自然保护区内3个垂直植被带的典型群落红松阔叶林、云冷杉林和岳桦林内,利用网袋埋藏法对群落内的6个主要优势乔木树种凋落物进行埋藏分解试验,研究凋落物分解速率及其变化动态;同时利用分解模型,模拟预测凋落物的分解进展,为深入研究这6个树种的营养策略、群落养分循环等奠定基础,也为森林生态系统管理提供理论依据。研究结果表明,所研究的6个树种凋落物都表现出随时间进程失重率增大的现象,但失重率并不与时间呈线性相关。在分解的638d(1.75a)后,6种叶凋落物的分解速率明显升高。到分解实验结止时(699d),叶凋落物干重剩余率从小至大依次为白桦(24.56%)、紫椴(24.81%)、红松(38.48%)、鱼鳞云杉(41.15%)、岳桦(41.53%)和臭冷杉(42.62%)。枝凋落物分解速率明显低于叶,枝干重剩余率从小至大依次为紫椴(44.98%)、臭冷杉(64.62%)、红松(72.07%)、鱼鳞云杉(73.51%)、白桦(77.37%)和岳桦(80.35%)。在同一海拔高度,阔叶树种叶凋落物分解速率大于针叶树种。并且随着海拔的升高,叶凋落物分解速率逐渐减慢。模型分析预测结果表明,长白山北坡各垂直植被带的优势树种叶凋落物分解95%需4.5~8.0a;年分解系数为紫椴(0.686)>白桦(0.624)>红松(0.441)>鱼鳞云杉(0.406)>臭冷杉(0.397)>岳桦(0.385);枝凋落物分解95%需7.8~29.3a,不同树种间的差异明显。枝年分解系数为紫椴(0.391)>臭冷杉(0.204)>红松(0.176)>鱼鳞云杉(0.157)>白桦(0.148)>岳桦(0.102)。