989 resultados para rendimento clonal
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The objective of this study was to evaluate the processing methods (F-1 = to remove skin with pliers and then to cut in fillets; F-2 = cut in fillet and then to remove skin with knife and pliers help) and weight categories (W-1=250-300 g; W-2=301-350 g; W-3 = 351-400 g and W-4 = 401-450 g), on the carcass (CY), fillet (FY) and skin yield of Nile tilapia. Forty-eight fishes were used in a completely randomized design. There was effect for the processing method, being the F-1 mean (56.43 and 36.67 %) higher to the F-2 (53.46 and 32.89%) for CY and FY respectively. For the weight categories, W-1 (56.49 and 37.34%) and W-2 (56.34 and 36.40%) were superior as compared to W-3 (53.27 and 31.98%) and W-4 (53.71 and 33.42%), respectively for CY and FY. Crude skin percentage, clean and of fleshed were higher for F-2, but there was no effect for weight categories. The F-1 processing method promoted the best yield and skin results, and for the weight categories W-1 and W-2 higher yields.
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The objective of the present work was evaluate two techniques of smoking, the hot traditional and with use of smoke natural aroma or "liquid smoke", having as control Perna perna mussels vapor cooked. Sensorial characteristics were appraised (appearance, color, aroma, texture, flavor and salt tenor) and yield processing. The experimental design was completely randomized with three treatments (T1 = smoked with liquid smoke; T2 = traditional smoked and T3 = just cooked, control), with nine repetitions. For sensorial analysis 30 people were used, where each one was considered a block. There was significant difference for processing performance among treatments (P<0.05), however there was not significant difference independent of smoked technique applied. Nevertheless, smoking technique using smoke natural aroma was more attractive, presenting better results with relationship to sensorial characteristics and final product packaged for commercialization.
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2009
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Case Description: An 82-years old Hispanic woman with a past medical history significant for pulmonary thromboembolism on oral anticoagulation, rheumatoid arthritis, and hypertension developed a new onset thrombocytopenia. Clinical Findings: Small clonal B-cells populations (SCBP) also known as monoclonal B-cell lymphocytosis was found as part of the workup for an idiopathic thrombocytopenia and lead ultimately to the diagnosis of parotid primary follicular lymphoma coexisting with Warthin tumor involving the bone marrow in a small extent and oncocytic papilloma located in the maxillary sinus. Treatment and Outcome: Patient was treated with Rituximab monotherapy with improvement on her platelet count. Clinical relevance: Although it is unclear the role of this clonal cells, they may work as a possible diagnostic tool for occult lymphomas. Further prospective studies are needed to confirm this possible association.
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The technique of delineating Populus tremuloides (Michx.) clonal colonies based on morphology and phenology has been utilized in many studies and forestry applications since the 1950s. Recently, the availability and robustness of molecular markers has challenged the validity of such approaches for accurate clonal identification. However, genetically sampling an entire stand is largely impractical or impossible. For that reason, it is often necessary to delineate putative genet boundaries for a more selective approach when genetically analyzing a clonal population. Here I re-evaluated the usefulness of phenotypic delineation by: (1) genetically identifying clonal colonies using nuclear microsatellite markers, (2) assessing phenotypic inter- and intraclonal agreement, and (3) determining the accuracy of visible characters to correctly assign ramets to their respective genets. The long-term soil productivity study plot 28 was chosen for analysis and is located in the Ottawa National Forest, MI (46° 37'60.0" N, 89° 12'42.7" W). In total, 32 genets were identified from 181 stems using seven microsatellite markers. The average genet size was 5.5 ramets and six of the largest were selected for phenotypic analyses. Phenotypic analyses included budbreak timing, DBH, bark thickness, bark color or brightness, leaf senescence, leaf serrations, and leaf length ratio. All phenotypic characters, except for DBH, were useful for the analysis of inter- and intraclonal variation and phenotypic delineation. Generally, phenotypic expression was related to genotype with multiple response permutation procedure (MRPP) intraclonal distance values ranging from 0.148 and 0.427 and an observed MRPP delta value=0.221 when the expected delta=0.5. The phenotypic traits, though, overlapped significantly among some clones. When stems were assigned into phenotypic groups, six phenotypic groups were identified with each group containing a dominant genotype or clonal colony. All phenotypic groups contained stems from at least two clonal colonies and no clonal colony was entirely contained within one phenotypic group. These results demonstrate that phenotype varies with genotype and stand clonality can be determined using phenotypic characters, but phenotypic delineation is less precise. I therefore recommend that some genetic identification follow any phenotypic delineation. The amount of genetic identification required for clonal confirmation is likely to vary based on stand and environmental conditions. Further analysis, however, is needed to test these findings in other forest stands and populations.
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Il mio elaborato si pone come obiettivo il miglioramento di una macchina di condizionamento atta al funzionamento a temperature inferiori allo standard nazionale (circa -20°C, -253,15 K). Il lavoro si basa sullo studio di un sistema innovativo di sbrinamento per pompe di calore ad alta efficienza che necessiti nel minor modo possibile di manutenzione, sia di facile installazione e permetta alla macchina di mantenere il rendimento intorno a valori accettabili. Dopo aver portato avanti un'analisi energetica del sistema, attraverso lo studio di diverse possibilità di sbrinamento, l'elaborato verifica che l'installazione del sistema progettato porti ad un effettivo vantaggio anche dal punto di vista economico.
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In questo lavoro si analizza il rendimento isoentropico di diversi espansori adottati all’interno di sistemi micro-ORC (Organic Rankine Cycle). Si parte da una descrizione generale dei sistemi ORC, mettendone in evidenza le principali caratteristiche, il layout tipico, le differenze rispetto ad un ciclo Rankine tradizionale, e gli ambiti di utilizzo. Si procede ad una trattazione teorica del rendimento isoentropico del compressore e dell’espansore, specificando le ipotesi adottate nei calcoli e mettendo in luce la relazione tra rendimento isoentropico e politropico nell’uno e nell’altro apparato. Si passa poi alla descrizione delle quattro principali tipologie di espansori presenti in letteratura: scroll, screw, vane e piston, e si prosegue con l'analisi nel dettaglio della letteratura relativa alla valutazione dell’efficienza di questi quando utilizzati all’interno di un sistema micro-ORC. Infine, dopo aver descritto il sistema ORC del laboratorio di via Terracini del DIN, illustrandone layout, componenti principali, potenza scambiata all’interno dei componenti e modalità di calcolo adottata per la valutazione del rendimento isoentropico dell’espansore e del sistema complessivo, si confrontano i dati di efficienza di questo con quelli reperiti in letteratura. Il confronto del rendimento, dell'espansore e complessivo, del sistema del DIN, 33.8% e 1,818% rispettivamente, con quelli degli altri sistemi, è risultato di difficilmente valutazione a causa delle condizioni di forte off-design del sistema stesso. Dalla ricerca è inoltre emerso che i fluidi più utilizzati nella sperimentazione, e dunque capaci di migliori prestazioni, sono R245fa, R134a e R123. Per quel che riguarda infine gli espansori, nel range di potenza di 1-10 kW, lo scroll risulta essere il migliore.
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Pandemic methicillin-resistant Staphylococcus aureus (MRSA) clonal complex 97 (CC97) lineages originated from livestock-to-human host jumps. In recent years, CC97 has become one of the major MRSA lineages detected in Italian farmed animals. The aim of this study was to characterize and analyze differences in MRSA and methicillin-susceptible S. aureus (MSSA) mainly of swine and bovine origins. Forty-seven CC97 isolates, 35 MRSA isolates, and 6 MSSA isolates from different Italian pig and cattle holdings; 5 pig MRSA isolates from Germany; and 1 human MSSA isolate from Spain were characterized by macrorestriction pulsed-field gel electrophoresis (PFGE) analysis, multilocus sequence typing (MLST), spa typing, staphylococcal cassette chromosome mec (SCCmec) typing, and antimicrobial resistance pattern analysis. Virulence and resistance genes were investigated by PCR and microarray analysis. Most of the isolates were of SCCmec type V (SCCmec V), except for two German MRSA isolates (SCCmec III). Five main clusters were identified by PFGE, with the German isolates (clusters I and II) showing 60.5% similarity with the Italian isolates, most of which (68.1%) grouped into cluster V. All CC97 isolates were Panton-Valentine leukocidin (PVL) negative, and a few (n = 7) tested positive for sak or scn. All MRSA isolates were multidrug resistant (MDR), and the main features were erm(B)- or erm(C)-mediated (n = 18) macrolide-lincosamide-streptogramin B resistance, vga(A)-mediated (n = 37) pleuromutilin resistance, fluoroquinolone resistance (n = 33), tet(K) in 32/37 tet(M)-positive isolates, and blaZ in almost all MRSA isolates. Few host-associated differences were detected among CC97 MRSA isolates: their extensive MDR nature in both pigs and dairy cattle may be a consequence of a spillback from pigs of a MRSA lineage that originated in cattle as MSSA and needs further investigation. Measures should be implemented at the farm level to prevent spillover to humans in intensive farming areas.
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Mycobacterium bovis populations in countries with persistent bovine tuberculosis usually show a prevalent spoligotype with a wide geographical distribution. This study applied mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (MIRU-VNTR) typing to a random panel of 115 M. bovis isolates that are representative of the most frequent spoligotype in the Iberian Peninsula, SB0121. VNTR typing targeted nine loci: ETR-A (alias VNTR2165), ETR-B (VNTR2461), ETR-D (MIRU4, VNTR580), ETR-E (MIRU31, VNTR3192), MIRU26 (VNTR2996), QUB11a (VNTR2163a), QUB11b (VNTR2163b), QUB26 (VNTR4052), and QUB3232 (VNTR3232). We found a high degree of diversity among the studied isolates (discriminatory index [D] = 0.9856), which were split into 65 different MIRU-VNTR types. An alternative short-format MIRU-VNTR typing targeting only the four loci with the highest variability values was found to offer an equivalent discriminatory index. Minimum spanning trees using the MIRU-VNTR data showed the hypothetical evolution of an apparent clonal group. MIRU-VNTR analysis was also applied to the isolates of 176 animals from 15 farms infected by M. bovis SB0121; in 10 farms, the analysis revealed the coexistence of two to five different MIRU types differing in one to six loci, which highlights the frequency of undetected heterogeneity.
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We have identified a clonal complex of Mycobacterium bovis isolated at high frequency from cattle in Uganda, Burundi, Tanzania, and Ethiopia. We have named this related group of M. bovis strains the African 2 (Af2) clonal complex of M. bovis. Af2 strains are defined by a specific chromosomal deletion (RDAf2) and can be identified by the absence of spacers 3 to 7 in their spoligotype patterns. Deletion analysis of M. bovis isolates from Algeria, Mali, Chad, Nigeria, Cameroon, South Africa, and Mozambique did not identify any strains of the Af2 clonal complex, suggesting that this clonal complex of M. bovis is localized in East Africa. The specific spoligotype pattern of the Af2 clonal complex was rarely identified among isolates from outside Africa, and the few isolates that were found and tested were intact at the RDAf2 locus. We conclude that the Af2 clonal complex is localized to cattle in East Africa. We found that strains of the Af2 clonal complex of M. bovis have, in general, four or more copies of the insertion sequence IS6110, in contrast to the majority of M. bovis strains isolated from cattle, which are thought to carry only one or a few copies.
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TET2 is a tumor suppressor gene that has been implicated in the epigenetic regulation of gene expression. Inactivating TET2 mutations are common in MDS. These mutations may contribute to early clonal dominance and myeloid transformation, although the exact mechanisms remain to be elucidated. Common to the environment of MDS are elevations in cytokines, such as TNFα and IFN-γ. It was hypothesized that inflammatory cytokines TNF-α and IFN-γ may promote clonal expansion of TET2 mutant progenitors. Adult (10-14 weeks-old) Tet2 wild type (+/+) and Tet2 mutant (-/-) C57BL/6 mice strains were chosen as a model system. Lineage negative cells (Lin-), enriched for hematopoietic stem and progenitor cells, were isolated from Tet2 +/+ and -/- bone marrow and cultured in the absence or presence of varying concentrations of TNFα or IFN-γ in methylcellulose colony formation assays and long term cell culture assays, over a period of 12 and 30 days respectively, and their colony growth, cell count, immunophenotype and resistance to apoptosis were examined. Where indicated, serial re-plating was performed. Expression of apoptotic regulators was assessed by qRT-PCR. In the triplicate experiments, starting with equal densities of Tet2 +/+ and -/- Lin- cells, Tet2 -/- Lin- cells displayed increased resistance to cytokine-induced growth suppression and superior colony forming ability over +/+ in the serial re-plating assays under stress of increasing TNFα or IFN γ. Tet2 -/- progenitors also displayed a lower apoptotic index compared to +/+ under stress of increasing TNFα, suggesting increased resistance to TNFα induced apoptosis. Transcriptional data showed low expression of Tnfr1, Fas and caspase 8, as well as a high expression of Bcl-2 and Iap1 in Tet2 -/- compared to +/+ under stress of TNFα. Tet2-/- also showed increased basal expression of endogenous TNFα mRNA compared to +/+. In the human colony growth assay, the clonal growth of TET2 mutant CFU-GM progenitors was enhanced at low TNFα concentrations. Conclusion: Mutations that promote resistance to environmental stem cell stressors are a known mechanism of clonal selection in aplastic anaemia and JAK2-mutant MPN and our findings suggest that this mechanism may be critical to clonal selection and dominance in MDS.
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A presente dissertação de mestrado tem como finalidade primordial mapear a intervenção técnica com base processual, junto de uma família beneficiária de rendimento social de inserção. Mais especificamente aferir as problemáticas e vulnerabilidades da família ao longo do acompanhamento, confrontando com a avaliação diagnóstica e a elaboração de todo o acompanhamento. A investigação segue uma metodologia de estudo de caso com análise documental de um processo acompanhado há 18 anos, cruzando as evidências da intervenção aferindo avaliação diagnostica, a conceção do plano de intervenção e respetivo processo de monitorização, acompanhamento e avaliação. O presente estudo apresenta a necessidade de estruturação de instrumentos de monitorização de todo acompanhamento e uma reflexão sobre as implicações da medida relativamente ao comportamento das famílias beneficiária.
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O presente trabalho tem como objetivo analisar em que medidas as Culturas da Qualidade facilitam o sucesso de implementação da técnica de gestão Benchmarkting, bem como o impacto destas duas variáveis no Rendimento das organizações. A Industria Têxtil e Vestuário portuguesa é a área de investigação escolhida para analise empírica de um modelo de investigação formado por 7 hipóteses. Todas as hipóteses foram elaboradas após uma exausta da literatura sobre Benchmarkting, Culturas da Empresa na Gestão da Qualidade, e em breve enquadramento da Industria Têxtil em Portugal. No sentido de dar resposta ás correlações supra mencionadas, entre "Culturas de Deteção de Erros, Cultura de Prevenção de Erros, Cultura e Melhoria Continua, Bencharmarkting e Rendimento", desenvolveu-se um questionário já estado em outros estudos análogos(Cameron e Sine, 1999; Costa, 2008; Leal, Roldan e Acedo, 2002). Este foi distribuído por uma amostra de 60 empresas na região norte do país. Dos resultados obtidos, verificou-se a existência de uma Cultura de Qualidade dominante(Cultura de Deteção de Erros), sinónimos de que as Industrias Têxteis portuguesas ainda se orientam popr valores de qualidade ultrapassados, senão obsoletos. De realçar também, uma elevada percentagem de Industrias Têxteis que não aplicam o Benchmarkting. Porém, os resultados revelam também a existência de correlações fortes quanto: Á Cultura Deteção de Erros influenciar negativamente o Benchmarkting; á Cultura Criativa e Melhoria Continua influenciar positivamente o Benchmarkting; á Cultura de deteção de erros influenciar negativamente o Rendimento; á Cultura Criativa e Melhoria Continua influenciar o Rendimento; ao Benchmarkting influenciar positivamente o Rendimento. No final, na verificação de resultados e conclusões, efetuam-se positivamente algumas recomendações que podem ser úteis para a gestão das Industrias têxteis portuguesas.
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Neste trabalho, estudamos a problemática fiscal dos serviços intragrupo em imposto sobre o rendimento das sociedades. Esta questão tem vindo a ser analisada por organizações internacionais, como a Organização para a Cooperação e o Desenvolvimento Económico (OCDE) e pela União Europeia, devido à sua importância na prossecução um objetivo comum aos Estados interessados no comércio global que é a eliminação das barreiras fiscais às atividades económicas transfronteiriças. Com efeito, com a internacionalização das empresas, surgem cada vez mais transações de bens e serviços entre empresas interligadas, tradicionalmente identificadas como empresas pertencentes a um grupo económico, mas situadas em países diferentes. Os grupos económicos, numa tentativa de redução dos custos e de obtenção de economias de escala, tendem a centralizar certas atividades não diretamente relacionadas com o objeto social dos membros, como sejam, de natureza administrativa, técnica, financeira, comercial etc., num dos membros - normalmente a sociedade matriz. Este membro presta esses serviços aos restantes membros do grupo, - daí a designação de “serviços intragrupo”-, em troca de uma remuneração calculada de acordo com o princípio de plena concorrência Essa centralização de serviços num dos membros, com a imputação dos respetivos custos ou gastos ao membro, considerado o real beneficiário dos mesmos serviços, constituem o essencial da problemática fiscal dos serviços intragrupo, no que respeita ao imposto sobre o rendimento. Considera-se que apenas o beneficiário efetivo do serviço pode deduzir fiscalmente o custo do serviço intragrupo. E havendo divergências entre as Administrações Tributárias dos Estados onde estão estabelecidas as sociedades do grupo quanto ao beneficiário efetivo do serviço, nenhuma das Administrações Tributárias permitirá a dedução fiscal do custo do serviço prestado. Consequentemente o grupo sofrerá uma dupla tributação, o que constitui um obstáculo às atividades económicas transfronteiriças para grupos económicos. Com base no princípio de plena concorrência, coloca-se a questão de saber em que medida os serviços intragrupo constituem um benefício efetivo para o grupo, quer no seu conjunto, quer para cada membro individualmente considerado, de forma a cumprir com os pressupostos para a dedução dos respetivos custos no cálculo do imposto sobre o rendimento dos membros.
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O presente estudo partiu de uma amostra de 472 estudantes, do 7º ao 9º ano de três escolas e duas entidades desportivas de Évora, e seis escolas de Lisboa. Procurou-se compreender qual o impacto que a prática de exercício físico tem na adolescência ao nível do bem-estar, autoestima e rendimento escolar através da aplicação da EBEPA e da Escala de Autoestima Global de Rosenberg. Objetivou-se também perceberquais as atitudes dos/as jovens face à educação física e ao exercício físico, através da aplicação do QAAEF e QAEFFCE. Os resultados obtidos sugerem que a prática de exercício físico extracurricular potencia os níveis de bem-estar, autoestima e rendimento escolar dos/as adolescentes. Ao praticarem exercício físico para além do proporcionado pelas aulas de educação física, estes jovens apresentam atitudes mais positivas no que diz respeito ao gosto e importância atribuídos à disciplina e à prática de exercício físico. Os rapazes apresentam resultados mais elevados do que as raparigas, no que diz respeito ao bem-estar e autoestima; Impact of physical exercise in adolescents: well-being, self-esteem and school performance Abstract: This study came from a sample of 472 students, from the 7th to the 9th grade of three schools and two sports entities of Évora, and six schools of Lisbon. We tried to understand the impact that the practice of physical exercise has in adolescence in terms of well-being, self-esteem and school performance through the application of the EBEPA and the Rosenberg Self-esteem Scale. The goal was also to understand the attitudes of young people in the face of physical education and physical exercise, through the application of QAAEF and QAEFFCE. The results suggest that the practice of extracurricular physical exercise increases levels of well-being, self-esteem and academic achievement of adolescents. By practice physical exercise,not counting physical education classes, these young people exhibit more positive attitudes giving more credit to the discipline and practice of physical exercise. This study confirmed that the boys have higher results than girls, with regard to the well-being and self-esteem.