Effects of microcystins on the growth and the activity of superoxide dismutase and peroxidase of rape (Brassica napus L.) and rice (Oryza sativa L.)

Microcystins are naturally occurring hepatotoxic cyclic heptapeptides produced by some toxic freshwater cyanobacterial species. In this study, crude extract of toxic cyanobacterial blooms from Dianchi Lake in southwestern China was used to determine the effects of microcystins on rape (Brassica napus L.) and rice (Oryza sativa L.). Experiments were carried out on a range of doses of the extract (equivalent to 0, 0.024, 0.12, 0.6 and 3 mug MC-LR/ml). Investigations showed that exposure to microcystins inhibited the growth and development of both rice and rape seedlings, however, microcystins had more powerful inhibition effect on rape than rice in germination percentage of seeds and seedling height. Microcystins significantly inhibited the elongation of primary roots of rape and rice seedlings. Determination of the activities of peroxidase and superoxide dismutase demonstrated that microcystin stress was manifested as an oxidative stress. Using ELISA, microcystins were examined from the extract of exposed rape and rice seedlings, indicating that consumption of edible plants exposed to microcystins via irrigation route may have health risks. Significantly different levels of recovered microcystins between exposed rice and rape seedlings Suggested that there might be different tolerant mechanisms toward microcystins. (C) 2004 Elsevier Ltd. All rights reserved.

New data on Myxobolus longisporus (Myxozoa : Myxobolidae), a gill infecting parasite of carp, Cyprinus carpio haematopterus, from Chinese lakes

The original description of Myxobolus longisporus Nie et Li, 1992, the species infecting gills of Cyprinus carpio haematopterus L., is supplemented with new data on the spore morphology and pathogenicity. Spores are elongate pyriform with pointed anterior end, 15.7 (15.5-16.5) mum long, 6.7 (6-8) mum wide and 5.5 mum thick. Sutural ridge is straight and narrow. Mucus envelope is lacking. Two equal-sized elongate pyriform polar capsules are 8.5 mum long and 2.5 mum wide with convergent long axes. Polar filament coiled perpendicularly to the long axis of the capsule makes 9 (8-10) turns. Posterior end of polar capsules exceeds mid-spore by 15-20%. Cyst-like plasmodia are localised in the gill secondary lamellae. The infection is described in adult big host specimens. Gross lesions manifested as dark red colouration of gill tissues were restricted to the ventral part of the first gill arches. Remarkable site specificity (apical part of secondary lamellae) was observed in the course of development of microscopic lesions. M. longisporus is characterised also on the molecular level using sequences of SSU rRNA gene. Phylogenetic analysis based on these sequences has allowed clearer phylogenetic relationships to be established with other species of the genus Myxobolus sequenced to date.

Detection of Myxobolus rotundus (Myxozoa : Myxosporea) in skin mucus of crucian carp Carassius auratus auratus using a monoclonal antibody

Diagnosis of myxosporean Myxobolus rotundus infection was conducted by examining skin mucus from the infected crucian carp Carassius auratus auratus with a monoclonal antibody, MAb 2D12, raised previously against the parasite. A positive reaction was observed in skin mucus collected from infected fish, and spores and pre-spore stages of the parasite were identified by the MAb 2D12. It was also demonstrated that M. rotundus infection can be successfully detected by a simple method, enzyme-linked immunosorbent assay (ELISA), and that skin mucus collected from infected fish skin had a significantly higher optical density (OD) value than that from uninfected fish.

子午岭辽东栎天然林土壤种子库研究

结合野外调查与室内试验对子午岭辽东栎天然林的种群生长状况,枯落层种子库,不同坡位土壤种子库物种组成、数量特征、生活型及其与地上植被的相似性进行了较为系统的研究。结果表明:(1)试验萌发鉴定出的幼苗共有24种,隶属于16科,其中多年生草本和落叶灌木物种比例最高,分别占种子库物种总数的58.33%和12.5%;(2)整个子午岭天然辽东栎林土壤种子库总密度为12 761.44粒/m2,种子主要分布在枯枝落叶层和0-2.5 cm土层中,其中,辽东栎种子密度为752.5粒/m2,占整个样地土壤种子库总密度的5.89%,表明辽东栎种子萌发能力很差,该林分群落天然更新缓慢。(3)子午岭天然辽东栎林的下坡位物种多样性指数较其它坡位高,但各个样地物种多样性指数均高于对照;(4)天然辽东栎纯林的种群生长状况良好,树龄均为成年树种;(5)方差分析表明:辽东栎的胸径、地径在不同坡位之间均具有显著性差异(P>0.05);(6)相关分析表明:枯枝落叶层厚度,重量与土壤种子库密度之间具无显著相关性(P<0.05)。

Hg胁迫对两种基因型小麦生长及其过氧化物酶活性的影响

用不同浓度Hg处理两种基因型小麦种子,较低浓度的Hg对小麦种子萌发影响比较小,对抗旱品种的小麦种子(陕合)的萌发有略微的刺激作用。小剂量、短时间的重金属处理可以提高POD的活性,发芽后受到Hg胁迫的陕合对Hg的耐受性低于发芽前就受到Hg胁迫的陕合,也低于同样胁迫处理的小麦品种(郑引)。发芽后进行Hg胁迫处理情况下,陕合对于Hg胁迫比较敏感,POD活性随着Hg浓度的升高而下降;而郑引,低浓度Hg对其POD活性有促进作用。在小麦发芽前就受到Hg胁迫的情况下,陕合和郑引的POD活性都随着Hg浓度增加表现为先上升而后下降趋势。

黄土丘陵沟壑区退耕地土壤种子库特征与季节动态

采用野外样线取样与室内萌发相结合的方法研究了黄土高原丘陵沟壑区退耕地种子库的组成、密度、分布特征、季节动态、种子库类型。结果表明,土壤种子库中共发现50种植物的种子,隶属15个科,39个属,主要物种组成与密度季节间差异不显著;在演替过程中土壤种子库的物种数量与密度随退耕年限的增加表现出:增加-减少-增加的趋势,同时阳坡种子库密度波动较阴坡剧烈;土壤种子库密度年内变化范围为1 067~14 717粒/m2,土壤种子库平均密度是夏季>秋季>春季;研究区域退耕地土壤种子库类型属于以猪毛蒿为优势种,并具有其他一年生、多年生草本植物与灌丛种子的没有显著的季节动态的持久种子库,是物种适应黄土丘陵沟壑区特有生态环境条件的繁殖更新策略;从土壤种子库的物种组成、密度、类型来看,黄土丘陵沟壑区退耕地具有一定的自然恢复潜力。

黄土丘陵植被恢复过程中土壤种子库萌发数量特征及动态变化

采用野外定点采样与室内土壤种子萌发试验相结合的研究方法,选取黄土丘陵区典型草原带宁夏固原地区不同植被恢复年限(2,5,12,24,35,55,75 a)及当年农地(对照)的8个样地,研究该区土壤种子库萌发数量特征及动态变化。结果表明,8个不同植被恢复年限样地土壤中可萌发的种子的平均数量为3542.5粒/m2,可萌发种子以双子叶植物为主,约占萌发总数的90%。0~5 cm土层可萌发种子数量变化于166.7~9100.0粒/m2;5~10 cm土层变化于133.38~2120.02粒/m2。土壤中可萌发种子数量随着恢复年限的增加表现先增加后下降的趋势。土壤中可萌发的种子数量随时间的变化近似单峰型,双子叶植物峰值主要出现在第4~6周,而单子叶植物萌发高峰在5~6周。表明在环境,气候等条件适宜时,黄土丘陵区典型草原带可萌发的土壤中的种子可形成萌发高峰。

不同年代冬小麦品种根际土浸提液诱导小列当种子发芽的化感作用研究

为筛选在根寄生植物小列当多发地进行诱捕发芽的小麦品种,研究了不同品种冬小麦根分泌的化感物质对小列当(Orobanche minor)种子的诱导发芽率。以冬小麦根际土蒸馏水和甲醇浸提液刺激小列当种子发芽,结果表明,冬小麦品种间化感作用差异显著。20世纪70年代宁冬1号和90年代小偃22号在4个生育期都有较强的发芽刺激作用,而其余3个品种则在个别生育期有非常微弱化感作用。宁冬1号品种刺激小列当发芽率达到最高为42.8%,化感作用最强,可作为抑除小列当的首选品种。

子午岭60年辽东栎林种子质量与森林更新

辽东栎是我国暖温带落叶阔叶林地带的优势乔木树种之一。通过26年的定位监测,对子午岭辽东栎林种子质量消减、种子萌发与环境条件、实生苗时空分布动态以及对其森林更新的影响等方面进行了初步探讨。结果表明:辽东栎林在子午岭半阳坡、半阴坡和阴坡3种类型中,平均完好种子占种子总数的26.65%;霉变种子占18.72%;动物取食虫蛀种子在阴坡远高于半阴坡和半阳坡,占到种子总数的26.32%;已发芽的种子占其总数的28.31%,且半阴坡>半阳坡>阴坡。每年均有大量种子生产,但在生境与动物的共同作用下,种子数量和质量受到很大影响,多达73.35%的种子失去生命力,直接影响实生苗的形成;地表覆盖物虽能促进种子的快速发芽,但对成苗却是一个物理障碍,影响是负作用的,主要影响因子是地表覆盖物的厚度和含水量;在辽东栎林下虽有一定的实生幼苗分布,但数量极少,平均密度仅为140~120株/hm2,且不同立地条件差异显著,严重影响森林的天然更新。

聚乙二醇和低温胁迫对红豆草种子萌发影响的研究

在植物生长箱通过种子萌发试验,从种子发芽进程、发芽率和吸水量等方面研究了水分和低温胁迫对红豆草种子萌发的影响。用10%,15%,20%,25%,30%的聚乙二醇(PEG)溶液和5℃左右低温预处理红豆草种子,结果表明,不同浓度的聚乙二醇和5℃左右低温预处理可以缩短种子的平均发芽时间,提高发芽率;比较发现,聚乙二醇浓度30%和低温处理时间4 d时可促进红豆草种子的萌发。

A switch-on mechanism to activate maize ribosome-inactivating protein for targeting HIV-infected cells

Maize ribosome-inactivating protein (RIP) is a plant toxin that inactivates eukaryotic ribosomes by depurinating a specific adenine residue at the a-sarcin/ricin loop of 28S rRNA. Maize RIP is first produced as a proenzyme with a 25-amino acid internal inactivation region on the protein surface. During germination, proteolytic removal of this internal inactivation region generates the active heterodimeric maize RIP with full N-glycosidase activity. This naturally occurring switch-on mechanism provides an opportunity for targeting the cytotoxin to pathogen-infected cells. Here, we report the addition of HIV-1 protease recognition sequences to the internal inactivation region and the activation of the maize RIP variants by HIV-1 protease in vitro and in HIV-infected cells. Among the variants generated, two were cleaved efficiently by HIV-1 protease. The HIV-1 protease-activated variants showed enhanced N-glycosidase activity in vivo as compared to their un-activated counterparts. They also possessed potent inhibitory effect on p24 antigen production in human T cells infected by two HIV-1 strains. This switch-on strategy for activating the enzymatic activity of maize RIP in target cells provides a platform for combating pathogens with a specific protease.

水分亏缺和凋落物水浸液对云杉幼苗生长和种子萌发的影响

在人类活动导致全球变暖的前提下，由于全球气温的升高，地表水分加速向空中蒸发。从20世纪70年代至今，地球上严重干旱地区的面积几乎扩大了一倍。这一增长的一半可归因于气温升高而不是降雨量下降，因为实际上同期全球平均降水量还略有增长。干旱对陆地植物和农林生态系统产生深远影响，并已成为全球变化研究的一个重要方面。位于青藏高原东部的川西亚高山针叶林是研究气候变暖对陆地生态系统影响的重要森林类型。森林采伐迹地、人工林下和林窗环境作为目前该区人工造林和森林更新的重要生境，其截然不同的光环境对亚高山针叶林更新和森林动态有非常重要的影响。凋落物产生的化感物质可通过影响种子萌发和早期幼苗的定居而影响种群的建立和更新，而人工林和自然林物种以及更新速度的差异性也都受凋落物的影响。 云杉是川西亚高山针叶林群落的重要树种之一,在维持亚高山森林的景观格局和区域生态安全方面具有十分重要的作用，其自然更新能力及其影响机制一直是研究的热点问题。本试验以云杉种子和2年生幼苗为研究对象，从萌发、根尖形态、幼苗生长、光合作用、渗透调节和抗氧化能力等方面研究了不同光环境下水分亏缺和凋落物水浸液对云杉种子和幼苗生长的影响。旨在从更新的角度探讨亚高山针叶林自然更新的过程，其研究成果可在一定程度上为川西亚高山针叶林更新提供科学依据，同时也可为林业生产管理提供科学指导。主要研究结论如下： 水分亏缺在生长形态、光合作用、抗氧化能力、活性氧化对云杉幼苗都有显著影响。总体表现为，水分亏缺导致了云杉幼苗的高度、地径、单株总生物量降低，增加了地下部分的生长；水分亏缺显著降低了云杉叶片中相对含水量、光合色素、叶氮含量，净光合速率和最大量子产量(Fv/Fm)，提高了幼苗叶片中膜脂过氧化产物（MDA）的含量；水分亏缺提高了幼苗叶片中过氧化氢(H2O2)含量，超氧荫离子（O2-）生成速率以及脯氨酸和抗氧化系统的活性（ASA, SOD, CAT, POD, APX和GR）。从这些结果可知，植物在遭受水分亏缺导致的伤害时，其自身会形成防御策略，并通过改变形态和生理方面的特性以减轻害。但是，这种自我保护机制依然不能抵抗严重水分亏缺对植物的伤害。 模拟林下低光照条件显著增加单株植物的地上部分生长，尤其是其叶片的比叶面积（叶面积/叶干重），同时其光合色素含量和叶片相对含水量也显著增加，这些改变直接导致植株光合速率和生物量的增加。同时，与高光照水平相比，低光照幼苗的膜脂过氧化产物（MDA）和活性氧物质均较低，显示出低光照比高光照水平对植物的更低的氧化伤害。尽管低光照也导致大部分抗氧化酶活性降低，但这正显示出植物遭受低的氧化伤害，更印证了前面的结论。 凋落物水浸液影响了云杉种子的萌发和根系的生长，更在形态、光合作用、抗氧化能力、活性氧物质以及叶氮水平上显著影响了云杉幼苗，其中，以人工纯林凋落物的影响更有强烈。具体表现在，种子萌发速率和萌发种子幼根的长度表现为对照>自然林处理>人工纯林；凋落物水浸液抑制种子分生区和伸长区的生长，人工林处理更降低了根毛区的生长，使根吸水分和养分困难。对2年生幼苗的影响主要表现在叶绿素含量、光合速率以及叶氮含量的降低；膜脂过氧化产物、活性氧物质和抗氧化酶系统的显著增加。同样的，人工纯林处理对云杉幼苗的影响显著于自然林处理。 在自然生态系统中，由于全球变暖气温升高导致的水分亏缺和森林凋落物都存在森林的砍伐迹地，林窗和林下环境中。我们的研究表明，与迹地或林窗强光照比较，林下的低光照环境由于为植物的生长营造了较为湿润的微环境，因此水分亏缺在林下对云杉幼苗造成的影响微弱。这可以从植物的形态、光合速率以及生物量积累，过氧化伤害和抗氧化酶系统表现出来。另一方面，凋落物水浸液在模拟林下低光照环境对植物的伤害也微弱于强光照环境，这与强光照环境高的水分散失导致环境水分亏缺有关；而人工纯林处理对云杉幼苗的伤害比对照和自然林处理显示出强烈的抑制作用。 Under the pre-condition of global warming resulted from intensive human activities, water in the earth’s surface rapidly evaporates due to the increase of global air temperature. From 1970s up to now, the area of serious drought in the world is almost twice as ever. This increase might be due to the increasing air temperature and not decreasing rainfall because global average rainfall in the corresponding period slightly is incremental. Drought will have profound impacts on terrestrial and agriculture-forest system and has also become the important issue of global change research. The subalpine coniferous forests in the eastern Qinghai-Tibet Plateau provide a natural laboratory for the studying the effects of global warming on terrestrial ecosystems. The light environment significantly differs among cutting blanks, forest gap and understory, which is particularly important for plant regeneration and forest dynamics in the subalpine coniferous forests. Picea asperata is one of the keystone species of subalpine coniferouis forests in western China, and it is very important in preserving landscape structure and regional ecological security of subalpine forests. The natural regeneration capacities and influence mechanism of Picea asperata are always the hot topics. In the present study, the short-term effects of two light levels (100% of full sunlight and 15% of full sunlight), two watering regimes (100% of field capacity and 30% of field capacity), two litter aqueous extracts (primitive forest and plantation aqueous extracts) on the seed germination, early growth and physiological traits of Picea asperata were determined in the laboratory and natural greenhouse. The present study was undertaken so as to give a better understanding of the regeneration progress affected by water deficit, low light and litter aqueous extracts. Our results could provide insights into the effects of climate warming on community composition and regeneration behavior for the subalpine coniferous forest ecosystem processes, and provide scientific direction for the forest production and management. Water deficit had significant effects on growth, morphological, physiological and biochemical traits of Picea asperata seedlings. Water deficit resulted in the decrease in height, basal diameter, total biomass and increase in under-ground development; water deficit significantly reduced the needle relative water content, photosynthetic pigments, needle nitrogen concentration, net photosynthetic rate and the maximum potential quantum yield of photosynthesis (Fv/Fm), and increased the degree of lipid peroxidation (MDA) in Picea asperata seedlings; water deficit also increased the rate of superoxide radical (O2-) production, hydrogen peroxide (H2O2) content, free proline content and the activities of antioxidant systems (ASA, SOD, POD, CAT, APX and GR) in Picea asperata seedlings. These results indicated that some protective mechanism was formed when plants suffered from drought stress, but the protection could not counteract the harm resulting from the serious drought stress on them. Low light in the understory significantly increased seedling above-ground development, especially the species leaf area (SLA), and photosynthetic pigments and relative needle content. These changes resulted in the increase in net photosynthetic rate and total biomass. Moreover, the lower MDA content and active oxygen species (AOS) (H2O2 and O2-) in low light seedlings suggested that low light had weaker oxidative damage as compared to high light. Lower antioxidant enzymes activities in low light seedlings indicated the weaker oxidative damage on Picea asperata seedlings than high light seedlings, which was correlative with the changes in MDA and AOS. Litter aqueous extracts affected seed germination and root system of Picea asperata seedlings. Significant changes in growth, photosynthesis, antioxidant activities, active oxygen species and leaf nitrogen concentration were also found in Picea asperata seedlings, and plantation treatment showed the stronger effects on these traits than those in control and primitive forest treatment. The present results indicated that seed germination and radicle length parameters in control were superior to those in primitive forest treatment, and those of primitive forest treatment were superior to plantation treatment; litter aqueous extracts inhibited the meristematic and elongation zone, and plantation treatment caused a decrease in root hairs so as to be difficult in absorbing water and nutrient in root system. On the other hand, litter aqueous extracts significantly decreased chlorophyll content, net photosynthetic rate and leaf nitrogen concentration of Picea asperata seedlings; MDA, AOS and antioxidant system activities were significantly increased in Picea asperata seedlings. Similarly, plantation treatment had more significant effect on Picea asperata seedlings as compared to primitive forest treatment. In the nature ecosystem, water deficit resulted from elevating air temperature and litter aqueous extract may probably coexist in the cutting blank, forest gap and understory. Our present study showed that water deficit had weaker effects on low light seedlings in the understory as compared to high light seedlings in the cutting blank and forest gap. The fact was confirmed from seedlings growth, gas exchange and biomass accumulation, peroxidation and antioxidant systems. This might be due to that low light-reduced leaf and air temperatures, vapour-pressure deficit, and the oxidative stresses can aggravate the impact of drought under higher light. On the other hand, litter aqueous extracts in the low light had weaker effects on the Picea asperata seedlings than those at high light level, which might be correlative to the water evapotranspiration under high light. Moreover, plantation litter aqueous extracts showed stronger inhibition for seed germination and seedling growth than control and primitive forest treatments.

川西亚高山云杉人工林天然更新与生境异质性研究

川西亚高山针叶林是四川森林的主体，是长江上游重要的生态屏障。云杉作为亚高山针叶林人工更新的主要树种，已经在该地区形成了大面积的人工纯林。目前，许多云杉人工林分已经进入更新成熟龄，而这些人工林的持续更新却成为日益凸现的问题。探讨这些云杉人工林的自我更新潜力及云杉种子种群更新特点，可为培育后续森林资源提供科学依据。 本文以川西米亚罗亚高山60a云杉人工林为研究对象，并以该区域内相对稳定的植被群落——天然林为对照，采用种子收集器、土壤种子库筛选、室内外种子萌发实验及野外幼苗调查等方法，从异质性微生境的角度研究了种子雨下落之后，不同微生境对种子库、种子萌发、幼苗建立及分布这一前期更新过程的影响，得出如下结果： 1、通过对川西亚高山60a云杉人工林和天然林6年内种子雨雨量、形态特征、散步动态等的持续观测和综合比较可以发现，云杉林结实特点由于林木自身的特征存在着巨大的变动，2002年和2006年两个种子结实大年内，60a人工林种子雨强度分别达到1088.2 ± 52.3粒/m2和704.3 ± 48.9粒/m2，远大于天然林579.9 ± 28.9粒/m2 和507.5± 30.7粒/m2；且云杉林结实质量优于天然林。60a人工林结实量大，种子质量也最好，相对天然林来说对种群的天然更新以及群落的演替都有最大的贡献潜力。应该说，在川西亚高山云杉人工林的天然更新过程中，种源不是影响天然更新的因素。在种子结实大年里，达到更新成熟的云杉人工林有着优于该地区相对稳定植被群落——天然林的种源优势。至少在种子结实大年，种子供应不是该区域人工林天然更新的限制因子。 2、相对于天然林种子库，人工林种子库在种子萌发前能够有较多的有活力种子。虽然这其中有种子雨输入量有差别的因素存在，但两种林分种子库对种子的保存率的不同才是造成这种差异的主要因素。在人工林中，不同地被类型形成的微生境显著地影响了种子库中种子的密度、垂直分布。有地被物存在的微生境能够将种子雨的大部分截留在地被层中，成为幼苗出现的主要场所；同时不同的地被物对种子的保存情况存在显著的差异，苔藓和凋落物层能都较好地保持其中的种子，到种子萌发前，这两种种子库类型能分别为天然更新提供366.1粒/m2和302粒/m2的有效种子。从这点来看，林下地被物上的种子库能够为天然更新萌发阶段提供数量可观的物质基础。 3、种子的萌发和幼苗的定居是天然更新过程中种子库向幼苗库转化的关键环节。总的说来，米亚罗人工林区60a云杉人工林种子向幼苗的转化率十分低下，凋落物、苔藓、草本、裸地四种主要地被物以及天然林内种子/幼苗的转化率分别仅为2.22%、2.14%、0.57%、0.67%、1.05%。这种低的转化率成为云杉林天然更新的限制性因子。但在现有更新条件下，微生境对这一环节仍然显示出十分显著的影响，表现为凋落物和苔藓对现有更新的新幼苗的保存率高于其它类型及天然林。苔藓和凋落物在种子萌发，幼苗保存，以及幼苗分布上都要优于其它地被物类型；另外，微地形对天然更新过程的影响也很显著，凹立地上更适宜于种子的汇集、萌发和定居。 Subalpine coniferous forests dominate most parts of the forested areas in western Sichuan, and they are important ecological barriers in the upper reaches of the Yangtze River. Picea asperata is one of the keystone spruce species for re-afforestation after felling of the natural forests and there have been a total of ca. 13 000 ha of plantations dominate by this species established. Nowadays, many P. asperata plantations have reached reproductive maturity. However, continued regeneration becomes to an important problem in these plantations. Understanding their self-regeneration potential and the regeneration characteristics of seed populations in spruce plantations of these plantations can have some insights on the management of these plantations and the establishment of following forest resources. A subalpine 60a P. asperata plantation distributed in Miyaluo artificial forest area was studied in this paper, at the same time. Synchronously, a 150a natural spruce forest was studied as comparison. Using seed collecting traps, sieving method for soil seed bank, seed germination experiments and seedling investigations in the field, the effects of heterogeneous microsites on early natural regeneration processes after seed rain were studied, which included seed banks, seed germination, seedling establishment and distribution. The main results are as follows. 1. Through a 6-year long term investigation of seed rain intensities, characteristic, dispersal dynamics of 60a P. asperata plantation, we could concluded that the seed setting properties of 60a P. asperata plantation have a great variation for the characteristics trees. In the mast seed year of 2002 and 2006, the seed rain intensities of plantation was 1088.2 ± 52.3 seeds/m2 and 704.3 ± 48.9 seeds/m2 respectively, which were much greater than that of natural spruce forest (579.9 ± 28.9 seeds/m2 in 2002, and 507.5± 30.7 seeds/m2 in 2006). Furthermore, the quality of seed rain in P. asperata plantation was better than that of natural spruce forest. Contrasting with natural spruce forest, 60a P. asperata plantation has a greater potential on natural regeneration of P. asperata population and succession of community for the reason of greater seed rain intensities and better seed quality. We can confirm that seed source was not a limiting factor which influences the natural regeneration progress of P. asperata population distributed in subalpine mountain zone, at least in the mast seed year. 2. Contrasting with natural spruce forest, P. asperata population had more viable seeds in seed bank before the germination. Although the difference of seed rain intensities of two forests has effect on this phenomenon, the difference of seed conservation ability in two forests was the main factor. In the P. asperata plantation, the seed densities and seed vertical distribution pattern were significant effected by the microsites, which posed by different ground cover types. In other word, Microsite with ground covers can obstruct most seeds and keep them in ground cover layer from seed rain, and these ground covers would be the main site for seed occurrence. There was a significant difference about seeds conservation ability among these ground covers. Litter and moss could better conserve P. asperata seeds which distributed in this two covers. Seed banks exist in litter and moss ground cover types could respectively provide 302seed/m2 and 366.1seed/m2 for natural regeneration before the seed germination. From this point of view, we could conclude that ground covers can ensure considerable numbers of seeds for the germination process. 3. Seed germination and seed establishment are key steps that the seeds invert to seedlings in natural generation process. In sum, the seed/seeding transform rate in 60a P. asperata plantation distributed in Miyaluo artificial forest area is very low. the seed/seeding transform rates in litter, moss, herb, soil surface and natural spruce forest were 2.22%、2.14%、0.57%、0.67%、1.05%, respectively. The low transform rate become to a limiting factor of P. asperata natural regeneration process. However, under the existing conditions of natural regeneration, microsit still had significant effect on this transform. The states of Seed germination, new seedling conservation, and older seedling distribution in litter and moss were better than in any other ground cover type or natural spruce forest. In addition, the micro-relief has significant effect on natural regeneration process. Concave site was more suitable for seed collection, seed germination and seedling distribution.

花椒水浸液及酚酸类化感物质对作物种子萌发和幼苗生长的影响效应

花椒（Zanthoxylum piperitum）是川西干旱河谷地区的重要经济作物，化感作用是花椒连作障碍的原因之一。系统研究花椒化感作用有助于深入理解并最终解决花椒连作障碍。本文通过研究花椒叶、林下土壤浸提液及单一纯化感物质对花椒幼苗生长、苜蓿种子萌发及幼苗生长的影响作用，从生理生化角度揭示浸提液及纯化感物质的作用方式。通过室外和室内模拟实验，对浸提液及纯化感物质的化感效应进行比较，为花椒连作障碍的解决和化感作用机制的深入理解提供依据。主要结果如下： 1.花椒叶及林下土壤浸提液对地下生物量影响作用强与对地上生物量的化感效应，两种浸提液的化感效应强度不同，叶浸提液作用表现更显著。其中在Y6、Y8 、T6和T8处理时，花椒幼苗地下生物量分别降低了31.2%、32.1%、31.6%和31.7%。 2.两种浸提液均能显著影响花椒幼苗体内的保护性酶活性，总体说来，在高浓度下抑制各种抗氧化物酶活性，幼苗体内丙二醛含量增加，幼苗受害严重；在较低浓度下，各种保护性酶活性有所增加，丙二醛含量减少，幼苗伤害减轻。同时，不同月份里，各种酶的活性高低显著不同，9月份的活性显著低于7月份的酶活性。对于养分含量的影响，Y8、T8的影响强度最大，分别使碳元素含量降低了27.8%和30.8%，使钾元素含量降低了34.7%和25.6%。 3.花椒叶及林下土壤浸提液对苜蓿种子萌发及幼苗生长有化感作用，表现在最终萌发率、不同物质代谢及保护性酶活性的差异上。两种浸提液对苜蓿种子萌发过程中蛋白质的含量均无显著性影响，对淀粉和可溶性糖的影响作用类似，高浓度处理无明显化感效应，较低浓度处理显著降低二者在萌发苜蓿种子中的含量。Y2、Y4与T4处理分别使可溶性糖含量减少了32.3%、29.1%和18.8%，Y2与T2处理分别使淀粉含量降低了29.3%和26.8%。 4.苜蓿种子在4种单一化感物质最高浓度即10-3 mol•L-1处理下，萌发率显著降低，半数萌发时间推迟，随着处理浓度降低，抑制作用逐渐减弱，当降低到10-6 mol•L-1时，又能够表现出对苜蓿种子萌发的促进作用。 5.纯化感物质在10-6 mol•L-1时使苜蓿幼苗叶片的保护性酶活性显著升高，丙二醛含量显著降低；在10-3 mol•L-1时使苜蓿叶片中保护性酶活性显著降低，丙二醛含量增加，膜脂过氧化程度加重。 Zanthoxylum piperitum is one of the most important cash crops and has been extensively cultivated in Eastern Tibetan Plateau, especially in the fragile dry valley areas. Allelopathic effects could be a reason for Z. piperitum’s continuous cropping impediment. Systemmatically research of the effect of Z. piperitum allelopathy could help to comprehend the continuous cropping impediment. The allelopathic effects on seedlings growth and seed germination of aqueous extracts of Zanthoxylum piperitum and phenolic allelochemicals were studied, and the action mechanism of the two substances was also discussed from physiology. Indoor and outdoor experiments were set to compare the difference between aqueous extracts and pure allelochemicals. The main results showed that: 1. The aqueous extracts of leaf and soil had significant allelopathic effects on aboveground and underground biomass, but the effect on underground biomass was stronger than the effect on underground evidently. Treated with Y6、Y8 、T6 and T8, the underground biomass was reduced 31.2%、32.1%、31.6% and 31.7% respectively. 2. The activity of activities of superoxide dismutase, catalase, peroxidase and ascorbate peroxidase were significantly reduced, while the content of MDA was increased and the seedlings were suffered stronger, when treated by the high concentration; but at the low concentration, these were reversed. And then, at the different month, the activities of antioxidant enzyme were significantly distinct. As for the contents of nutrient element, Y8、T8 had the more intensive effects than other treatments. 3. The results showed that the two types of aqueous extracts had significant allelopathic effects on seed germination, substances metabolize and the activity of antioxidant enzyme. But the aqueous extracts had no effects on the content of protein, while had the similar effects on the content of starch and soluble sugar. At Y2、Y4 and T4, the content of soluble sugar decreased 32.3%、29.1% and 18.8% respectively. 4. Treated with 10-3 mol•L-1 of the four allelochemicals, the seed germination of alfalfa was significantly inhibited. Ferulic acid, coumarin and vanillic acid at 10-3 mol•L-1 significantly reduced the activities of antioxidant enzyme, while the content of MDA in alfalfa seedling was significantly increased. The restrain effects became weakened with the treat concentration falled. However, ferulic acid, coumarin and vanillic acid could increase the activities of antioxidant enzyme at 10-6mol•L-1.

岷江上游高山林线附近优势树种土壤种子库和幼苗更新特征的研究

岷江上游地区高山/亚高山植被分布的坡向性分异显著，阴阳坡高山林线不仅物种组成差异明显，并且分布海拔呈现出阴坡高阳坡低的格局．阳坡林线树种主要是圆柏属乔木，林线类型多为渐变型，海拔高度大约在3 400m~3 800m；阴坡林线树种主要是冷杉，林线类型多为骤变型，海拔高度约在3 800m~4 400m．本研究采用土壤种子库物理筛选、室内萌发实验及野外群落调查等方法，对岷江上游地区阴坡岷江冷杉和阳坡祁连圆柏两类林线树种不同海拔梯度上土壤种子库以及幼苗库特征进行了调查，从土壤种子库和幼苗更新特征的角度对林线乔木树种种群更新特征进行了分析，进而对该地区高山林线在阴阳坡分布差异的原因进行了探讨，结果显示： 1．土壤种子库 阴坡：阴坡高山林线附近岷江冷杉土壤种子的平均密度大约为50.96粒/m2，其中树线以上10m处土壤种子密度为1.00粒/m2，树线处大约19.33粒/m2，林线交错带内土壤种子密度最高为136.83粒/m2，郁闭林内种子密度小于林线交错带，只有30.50粒/m2，种子平均空壳率为52％，霉变率达34%，完好种子只有6%．土壤种子库垂直分布特征为地被物层含种子比重最大，大约在67.50%左右；其次为0~2cm层，约18.84%左右；2~5cm层所占种子比例最小，约13.66%左右．霉变种子数量与土壤深度呈负相关． 阳坡：阳坡祁连圆柏土壤种子的平均密度为60.16粒/m2．树线以上10m处密度为1.92粒/m2，树线位置大约108.16粒/m2，林线交错带内平均为75.80粒/m2，郁闭林内种子密度小于林线交错带，只有20.00粒/m2．种子平均空壳率为36％，完好种子占49%，霉变率较低，大约为10%．阴阳坡林线树种土壤种子库垂直分布特征为：地被物层含种子最多，其次为0~4cm层，4~10cm层所占种子比例最小，霉变种子数量与土壤深度也呈负相关． 2． 幼苗库调查 阳坡：在树线以上区域没有发现幼苗，林线交错带内幼苗密度平均达3 250株/hm2，郁闭林内仅2 750株/ hm2．整个样地内1～2a幼苗很少甚至没有出现，3～10a的幼苗相对较多．空间分布上，祁连圆柏幼苗在林线交错带内接近随机分布，郁闭林内则介于随机分布和均匀分布之间． 阴坡：在树线以上幼苗密度为1 250株/ hm2，全部为1~2a幼苗，林线交错带内幼苗密度平均达7 000株/ hm2，郁闭林内达6 250株/ hm2．林线附近岷江冷杉幼苗丰富度以及幼苗的出现频率明显高于祁连圆柏，年龄结构也较祁连圆柏完整．岷江冷杉幼苗空间分布除了树线处幼苗的分布为随机分布，其他海拔则为集群分布． 3．从不同土壤深度的种子总量和幼苗数量的相关性检验发现，当年生幼苗数量跟表层种子总量相关性极显著, 但是两年生幼苗的数量与底层种子数量相关性显著．土壤种子在土壤中的垂直分布格局从一定程度上可以反映种子库的年际特征．岷江冷杉土壤种子库较丰富，种子散布后的存活力随着时间的变化逐渐下降，属于季节性瞬时种子库；祁连圆柏土壤种子散布格局为集群型分布，成熟种子大部分散布在母株冠幅内，属于永久性土壤种子库． 4．在阴坡林线交错带及以上区域还存在较为丰富的乔木土壤种子，并且在树线以上区域还发现了少量的岷江冷杉幼苗．从样地乔木的年龄结构发现，在林线交错带内上部到树线位置主要以幼龄林为主，且年龄结构完整，基本符合入侵性林线特征；阳坡林线交错带内幼苗出现频率很低，树线以上区域虽然存在种子库，但是没有幼苗出现，在林线交错带内乔木径级差距很大，年龄结构异常不完整，这种特征的林线将会面临两个可能结果：一种是维持现有状态，保持平衡；另外一种就是退化，但阳坡林线的实际动态趋势还有待长期定点研究． Treelines on the upper region of Minjiang River differ between the north aspect and the south aspect in their appearances, altitudes and tree species. On the north aspect, trees of Abies form a sharp and abrupt treeline ranging from 3800m to 4400m, while on the south the treeline is generally lower(3 400~3 800m), more open and gradual and mostly composed of Sabina. In this study, we examined the altitudinal gradients of soil seed banks and seedling recruitments at the treeline ecotones of a N-aspect and a S-aspect by using soil sieving, germination experiment and field investigations, analyzed the characteristics of population regeneration of tree species at the transitional zone and presented a analysis of the causes to the aspect-related difference in treeline patterns in the study area. Major results of our study include: 1． Soil seed bank N-aspect: Of the 50 plots investigated, the average density of soil seeds is 50.96/m2, in which well-formed seeds account for 6%, empty seeds 52%, parasitized seeds34%, and seeds damaged by animals 8%. The size of soil seed bank varies along altitude, being 1.00 seeds /m2 at the 10m above the treeline and ca.19.33 seeds/m2 at the upper limit of treeline. The highest density (136.83 seeds/m2) occurs at the treeline ecotone. By contrast, the density of soil seed for the closed forest is only 30.50 seeds/m2. In terms of vertical strata, 67.50% of the total seeds are at the surface layer, 18.84% at the middle layer (0~2cm) and 13.66% at deeper layer (2~5cm). The number of parasitized seeds is negatively correlated to soil depth. S-aspect: Of the 50 plots investigated, the average density of soil seeds is 60.16 seeds/m2, and the well-formed seeds account for 49%, empty seeds 36%, parasitized seeds10%, and seeds damaged by animals 1%. The size of soil seed bank varies along altitude, with 1.92 seeds/m2 recorded at the10m above the treeline，108.16 seeds/m2 at the upper limit of treeline, and 75.80 seeds/m2 at the treeline ecotone, while that for the closed forest is 20.00 seeds/m2. The number of seeds decreases with the depth of soil. As is on the N-aspect, the size of soil bank, from large to small, follows the order of the surface layer, the middle layer (0~4cm) and the bottom layer (4~10cm). The number of parasitized seeds is also negatively correlated to the depth of the soil. 2． Seedling bank N-aspect: A mean maximum seedling abundance of 31 000 seedlings/hm2 was recorded near alpine treeline at growing season. The density of seedlings is 1 250 seedlings/ha (all being 1 or 2 years old) at the alpine meadow 10m away above treeline, 7 000 seedlings/ha at treeline ecotone and 6 250 seedlings/ha for closed forest．The spatial distribution of Abies faxoniana seedlings is random at the upper limit of the treeline but clumped at other altitudes． S-aspect: No seedlings were found at the alpine meadow 10m away from the treeline. The density of seedlings was 3 250 seedlings/ha at treeline ecotone and 2 750 seedlings/ha for the closed forest．Hardly any 1 year current and 2 year-old seedlings appeared at the plots. The spatial distribution of Sabina przewalskii seedlings is random at treeline ecotone and between “random” and “even” forest closed forest． 3．Correlation tests of seedling population and seed bank at different soil layers indicated that the emergents were strongly correlated to seed bank at surface layer while the number of two-year seedlings was significantly correlated to the seed bank at the bottom of soil layer, indicating that germination mainly occurs at the soil surface while the middle or bottom layer was the reserve for non-germination or dead seeds. It can thus be postulated that Abies faxoniana soil seed bank is of seasonal transient type. By contrast, the soil seed bank of Sabina przewalskii is of persistent type and the soil seeds and seedlings of this species occurred more frequently near the islands of adult trees. 4．A good many soil seeds of both tree species were found near the treeline ecotone and above at N- and S-aspects. A few young seedlings were found above the Abies treeline. Investigation of five altitudinal transects respectively on N- and S-aspects indicated that Abies faxoniana has a more complete age structure than the stands of Sabina przewalskii. The age of firs decreased from closed forest to the upper limit of treeline, which suggests that the Abies treeline is advancing to higher altitude. While on the south aspect, only few Sabina przewalskii soil seeds and nearly no seedlings were found above the treeline ecotone. The stands exhibit extremely great difference in diameter classes with significantly incomplete age structure. This would lead to two possible results for the treelines: maintaining an equilibrium state at the current position or degenerating. But more studies should be carried out at longer time scales or larger spatial scales to understand whether the Sabina treeline is degenerating.