955 resultados para Six van Vromade, P. H.
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<p>Top Row: Caroline Vander Kuy, Dannielle Van Dommelen, Diane Fox, Catherine Winnell, Catherine Orsor, Alicia Hughesp><p>Row 2: Catharine Whorton, Lea Bell, Paula Durecki, Jennifer Jorissen, Marie Lewis, Sarah Bellestri, Margaret Van Boltp>
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<p>Back Row: ass't coach Yvette Harris, ass't coach Eileen Shea, Alayne Ingram, Infini Robinson, Susana Jara, Ruth Kipping, LeeAnn Bies, Raina Goodlow, Heather Oesterle, manager James Sanders, ass't coach Yulonda Wimbish-Gay, head coach Sue Guevarap><p>Front Row: manager Yolanda Cole, trainer Roxann Dahl, co-captain Anne Thorius, Alison Miller, co-captain Stacey Thomas, Kenisha Walker, Katie Dykhouse, student trainer Jamie Corl, manager Stephanie Van Eykp>
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<p>Front Row (left to right): assistant coach Mark Noetzel, diving coach Dick Kimball, William T. Hayes, Richard K. Wilkening, Brent D. Lang, Michael Barrowman, Eric Wunderlich, swimming coach Jon Urbanchek, assistant coach Bill Kopas p><p>Second Row: Eric Lesser, Noel Strauss, Steven Bigelow, Robert S. Silverman, Gregory Gooch, Thomas Andrew Stabile, Jr., Marc Milobinski, Thomas Hay, trainer Hank Handel p><p>Third Row: James Hume, Brian Gunn, Eric W. Wise, Eric Namesnik, Steve, Duttenhoffer, Stephen Hamerski, Bradley Lambert, William J. Harrisp><p>Back Row: Jarrett R. Winter, Richard Mitvalsky, Scott Van Appledornp>
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<p>Front Row (left to right): assistant coach Mark Noetzel, diving coach Dick Kimball, William T. Hayes, Richard K. Wilkening, Brent D. Lang, Michael Barrowman, Eric Wunderlich, swimming coach Jon Urbanchek, assistant coach Bill Kopas p><p>Second Row: Eric Lesser, Noel Strauss, Steven Bigelow, Robert S. Silverman, Gregory Gooch, Thomas Andrew Stabile, Jr., Marc Milobinski, Thomas Hay, trainer Hank Handel p><p>Third Row: James Hume, Brian Gunn, Eric W. Wise, Eric Namesnik, Steve, Duttenhoffer, Stephen Hamerski, Bradley Lambert, William J. Harrisp><p>Back Row: Jarrett R. Winter, Richard Mitvalsky, Scott Van Appledornp>
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The efficiency of physical separation of inclusion bodies from cell debris is related to cell debris size and inclusion body release and both factors should be taken into account when designing a process. In this work, cell disruption by enzymatic treatment with lysozyme and cellulase, by homogenization, and by homogenization with ammonia pretreatment is discussed. These disruption methods are compared on the basis of inclusion body release, operating costs, and cell debris particle size. The latter was measured with cumulative sedimentation analysis in combination with membrane-associated protein quantification by SDS-PAGE and a spectrophotometric pepticloglycan quantification method. Comparison of the results obtained with these two cell debris quantification methods shows that enzymatic treatment yields cell debris particles with varying chemical composition, while this is not the case with the other disruption methods that were investigated. Furthermore, the experiments show that ammonia pretreatment with homogenization increases inclusion body release compared to homogenization without pretreatment and that this pretreatment may be used to control the cell debris size to some extent. The enzymatic disruption process gives a higher product release than homogenization with or without ammonia pretreatment at lower operating costs, but it also yields a much smaller cell debris size than the other disruption process. This is unfavorable for centrifugal inclusion body purification in this case, where cell debris is the component going to the sediment and the inclusion body is the floating component. Nevertheless, calculations show that centrifugal separation of inclusion bodies from the enzymatically treated cells gives a high inclusion body yield and purity. (C) 2004 Wiley Periodicals, Inc.
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We present here a new approach to scalable quantum computing - a 'qubus computer' - which realizes qubit measurement and quantum gates through interacting qubits with a quantum communication bus mode. The qubits could be 'static' matter qubits or 'flying' optical qubits, but the scheme we focus on here is particularly suited to matter qubits. There is no requirement for direct interaction between the qubits. Universal two-qubit quantum gates may be effected by schemes which involve measurement of the bus mode, or by schemes where the bus disentangles automatically and no measurement is needed. In effect, the approach integrates together qubit degrees of freedom for computation with quantum continuous variables for communication and interaction.
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The Fe Mössbauer spectroscopy of mononuclear [Fe(II)(isoxazole)](ClO) has been studied to reveal the thermal spin crossover of Fe(II) between low-spin (S = 0) and high-spin (S = 2) states. Temperaturedependent spin transition curves have been constructed with the least-square fitted data obtained from the Mössbauer spectra measured at various temperatures between 84 and 270 K during a cooling and heating cycle. This compound exhibits an unusual temperature-dependent spin transition behaviour with T(?) = 223 and T(?) = 213 K occurring in the reverse order in comparison to those observed in SQUID observation and many other spin transition compounds. The compound has three high-spin Fe(II) sites at the highest temperature of study of which two undergo spin transitions. The compound seems to undergo a structural phase transition around the spin transition temperature, which plays a significant role in the spin crossover behaviour as well as the magnetic properties of the compound at temperatures below T. The present study reveals an increase in high-spin fraction upon heating in the temperature range below T, and an explanation is provided.
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There is a growing interest for esophageal measurements which can provide important and reliable data when diagnosing the motor function of the sphincters and the esophageal body. Biocompatibility, sensing resolution and the comfort of the patient are key parameters for manometric sensing systems. A new sensing approach which could fulfill all these needs is presented in this paper consisting of an embedded polymer fiber sensor, based on multiplexed fiber Bragg gratings. A response to a radial pressure almost 6 times that of a comparable silica fiber based sensor is obtained.
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There is a growing interest for esophageal measurements which can provide important and reliable data when diagnosing the motor function of the sphincters and the esophageal body. Biocompatibility, sensing resolution and the comfort of the patient are key parameters for manometric sensing systems. A new sensing approach which could fulfill all these needs is presented in this paper consisting of an embedded polymer fiber sensor, based on multiplexed fiber Bragg gratings. A response to a radial pressure almost 6 times that of a comparable silica fiber based sensor is obtained.
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Purpose: Defocus curves are used to evaluate the subjective range of clear vision of presbyopic corrections such as in eyes implanted with accommodating intraocular lenses (IOLs). This study determines whether letter sequences and/or lens presentation order ought to be randomised when measuring defocus curves. Methods: Defocus curves (range +2.00DS to -2.00DS) were measured on 18 pre-presbyopic subjects (mean age 24.1 ± 4.2 years) for six combinations of sequential or randomised positive or negative lens progression and non-randomised or randomised letter sequences. The letters were presented on a computerised logMAR chart at 6 m. Results: Overall there was a statistically significant difference between the six combinations (ANOVA, p < 0.05) attributable to the combination of non-randomised letters with non-randomised lens progression from negative to positive defocus (p < 0.01). There was no statistically significant difference in defocus curve measurements if both letters and lens order were randomised compared to if only one of these variables was randomised (p > 0.05). Non-randomised letters, with a sequential lens progression from negative to positive, was significantly different to all other combinations when compared individually (Student's T-test, p < 0.003 on all comparisons), and was confirmed as the sole source of the overall significant difference. There was no statistically significant difference if both lens presentation order and letter sequences were randomised compared to if only one or the other of these variables was randomised. Conclusion: Non-randomised letters and non-randomised lens progression on their own did not affect the subjective amplitude of accommodation as measured by defocus curves, although their combination should be avoided. © 2007 British Contact Lens Association.
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57Fe Mössbauer spectroscopy of the mononuclear [Fe(II)(isoxazole)6](BF4) 2compound has been studied to reveal the thermal spin crossover of Fe(II) between low-spin (S = 0) and high-spin (S = 2) states. A temperature-dependent spin transition curve has been constructed with the least-square fitted data obtained from the Mössbauer spectra measured at various temperatures in the 240-60K range during the cooling and heating cycle. The compound exhibits a temperature-dependent two-step spin transition phenomenon with Tsco (step 1) = 92 and Tsco (step2) = 191K. The compound has three high-spin Fe(II) sites at the highest temperature of study; among them, two have slightly different coordination environments. These two Fe(II) sites are found to undergo a spin transition, while the third Fe(II) site retains the high-spin state over the whole temperature range. Possible reasons for the formation of the two steps in the spin transition curve are discussed. The observations made from the present study are in complete agreement with those envisaged from earlier magnetic and structural studies made on [Fe(II)(isoxazole)6](BF4)2, but highlights the nature of the spin crossover mechanism.
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We cordially thank Alma Rute for excellent technical assistance and the DFG (GRK 1431-1 and 1431-2) for financial support (PB). We thank the Microscope and Histology Facility of the University of Aberdeen for providing their equipment. We also thank Jacob Hargreaves for proofreading the manuscript.
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Peer reviewed
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Peer reviewed
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Funded by UK Natural Environment Research Council European Commission. Grant Number: 227799 TOTAL Foundation MASTS pooling initiative (The Marine Alliance for Science and Technology for Scotland) Scottish Funding Council. Grant Number: HR09011