Liederbuch für die Mitglieder des Treubundes mit Gott für König und Vaterland

Boberach: Unter den Verfassern der 148 Lieder sind de la Motte Fouqué, Fontane, Arndt, Moritz Graf Strachwitz, Körner

Das verjüngte, oder vielmehr das zu verjüngende Deutschland : ein Büchlein für den lieben Bürgers- und Bauers-Mann

Boberach: Ein Rückblick auf die deutsche Geschichte lehrt, daß ein Oberhaus der Könige und Fürsten neben dem Volksparlament stehen muß. Auch der Kaiser vertritt das Volk. Der König von Preußen soll Kaiser werden. - Wentzke: Rückblick auf die deutsche Geschichte. Neben dem deutschen Volksparlament muß ein Oberhaus von Königen und Fürsten stehen. Endlich ein Kaiser als Vertreter des Volkes gegenüber dem Ausland und als vollziehende Gewalt, und zwar - der König von Preußen

Reden und Glossen

Boberach: Selbst in einer republikanischen Verfassung braucht der Adel, der seine historischen Verdienste hat, nicht abgeschafft zu werden. In der Polendebatte wollte Arndt die Teilungen mit dem Schutz vor Rußland begründen und dem Wahn wehren, die Polen könnten Deutschland vor Rußland schützen. Alle 10 Jahre sollen vom Parlament 10 Männer gewählt werden, die "über die geistigen, sittlichen, politischen Zustände" berichten und Vorschläge zu gesetzlichen Änderungen machen sollen. - Wentzke: S. 17 ff. Aus der Kölnischen Zeitung vom 28. März 1848: Verlangt ein deutsches Unterhaus und neben ihm ein Oberhaus der Fürsten. Der deutsche Kaiser und König hat 1. im Volks- und Fürstenparlament die Oberstimme wie auch in der Vertretung nach Außen, 2. unbeschränkten Befehl über Heer und Flotte. Im Frieden hat jeder Fürst in seinem Staat völlig selbständige Verwaltung und Heeresbefehl. S. 39 ff. Antrag vom 18. Juli 1848: Verlangt alle 10 Jahre Ernennung von Zensoren zur Untersuchung der geistigen, sittlichen und politischen Zustände Deutschlands. Der Reichsverweser soll die Macht und Majestät des Volkes darstellen, aber "sie blüht nur in Reichstagsbeschlüssen, es fehlt ihr Fleisch und Bein". Hinweis dagegen auf Preußen

Sendschreiben an meine Wähler

Welsch (Projektbearbeiter): Rechenschaftslegung des Berliner Verlagsbuchhändlers und Zeitungsverlegers Veit über seine politische Arbeit in der Frankfurter Nationalversammlung (18. Mai 1848 - 20. Mai 1849)

Caput I - V

Enth.: 1. Die Wiener Märtyrer. 2. Die symbolischen Bücher. 3. Traumbuch für Michel. 4. Eljen Kossuth! 5. Apostel und Apostaten

Functional proteomics and biochemistry: working together to unravel mitochondrial phenomena of African trypanosomes

Eukaryotic cells are compartmentalized into membrane-bound organelles in order to provide sheltered reaction rooms for various specific processes. Organelles are not randomly distributed in a cell or operate isolated from each other. At the contrary — some organelles are closely linked and their functions are tightly orchestrated. The most well-known example of two such organelles acting in concert are the ER and the mitochondrion that work together in order to coordinate cellular lipid biosynthesis, maintain Ca2+-homeostasis, regulate mitochondrial division and control mitochondrial/ER shape as well as to synchronize the movement of these organelles within a cell. To study the mitochondrion and its interface to the ER requires a simplified mitochondrial system. African trypanosomes represent such a system. The unicellular parasite that causes devastating diseases in humans and animals has only one large mitochondrion that does not undergo fission/fusion events except for the context of cell division. Moreover, mitochondrial functions and morphology are highly regulated throughout the life cycle of the protozoan. Central to the understanding of how mitochondria control their morphology, communicate with their surroundings and manage exchange of metabolites and transport of biopolymers (proteins, RNAs) is the mitochondrial outer membrane (MOM), as the MOM defines the boundary of the organelle. Recently, we have purified the MOM of T. brucei and characterized its proteome using label-free quantitative mass spectrometry for protein abundance profiling in combination with statistical analysis. Our results show that the trypanosomal MOM proteome consists of 82 proteins, two thirds of which have never been associated with mitochondria before. Among these, we identified novel factors required to regulate mitochondrial morphology and the long-elusive protein import machinery of T. brucei. A comparison with the MOM proteome of yeast defines a set of 17 common proteins that are likely present in the mitochondrial outer membrane of all eukaryotes. One of these is the Miro-GTPase Gem1. In yeast, this Ca2+-EF-Hand containing polypeptide is thought to be involved in a protein complex that physically tethers the mitochondrion to the ER. Interestingly, a putative tethering complex in mammalian cells was linked to the mitochondrial fusion/fission machinery. Thus, the concept of a protein complex-mediated connection seems to be a general and conserved feature. We are currently investigating, if such a protein complex exists in T. brucei and if the trypanosomal Gem1 protein is involved. This ER-subdomain associated with mitochondria has been termed mitochondria-associated ER-membranes or MAM. The MAM has recently been implicated to play a key role in Alzheimer’s disease. It is therefore of broad and general interest to establish other eukaryotic model systems in order to investigate the MAM-MOM connection in more detail.

Blätter der Erinnerung, meistens um und aus der Paulskirche in Frankfurt

Boberach: 82 "Stammbuchblätter und Sprüche" und 11 Gedichte u.a. auf Bassermann, den Tod Friedrich von Gagerns, Auerswalds und Lichnowskys, an Leubold, "Ihr Könige gebt Acht" und "Die Kaiserfahrt" verarbeiten Arndts Erlebnisse als Parlamentarier in der Fraktion im Weidenbusch

Rapamycin impairs endothelial cell function in human internal thoracic arteries.

BACKGROUND Definitive fate of the coronary endothelium after implantation of a drug-eluting stent remains unclear, but evidence has accumulated that treatment with rapamycin-eluting stents impairs endothelial function in human coronary arteries. The aim of our study was to demonstrate this phenomenon on functional, morphological and biochemical level in human internal thoracic arteries (ITA) serving as coronary artery model. METHODS After exposure to rapamycin for 20 h, functional activity of ITA rings was investigated using the organ bath technique. Morphological analysis was performed by scanning electron microscopy and evaluated by two independent observers in blinded fashion. For measurement of endothelial nitric oxide synthase (eNOS) release, mammalian target of rapamycin (mTOR) and protein kinase B (PKB) (Akt) activation, Western blotting on human mammary epithelial cells-1 and on ITA homogenates was performed. RESULTS Comparison of the acetylcholine-induced relaxation revealed a significant concentration-dependent decrease to 66 ± 7 % and 36 ± 7 % (mean ± SEM) after 20-h incubation with 1 and 10 μM rapamycin. Electron microscopic evaluation of the endothelial layer showed no differences between controls and samples exposed to 10 μM rapamycin. Western blots after 20-h incubation with rapamycin (10 nM-1 μM) revealed a significant and concentration-dependent reduction of p (Ser 1177)-eNOS (down to 38 ± 8 %) in human mammary epithelial cells (Hmec)-1. Furthermore, 1 μM rapamycin significantly reduced activation of p (Ser2481)-mTOR (58 ± 11 %), p (Ser2481)-mTOR (23 ± 4 %) and p (Ser473)-Akt (38 ± 6 %) in ITA homogenates leaving Akt protein levels unchanged. CONCLUSIONS The present data suggests that 20-h exposure of ITA rings to rapamycin reduces endothelium-mediated relaxation through down-regulation of Akt-phosphorylation via the mTOR signalling axis within the ITA tissue without injuring the endothelial cell layer.

Zeitgenossen in Biographieen und Porträts : ein Volksbuch

Boberach: Behandelt werden Alexander Freiherr von Soiron, Jakob Venedey, Oskar von Wydenbrugk, Gottlieb Christian Schüler, Heinrich Freiherr von Gagern, Ernst Moritz Arndt, Carl Theodor Welcker, Friedrich Ludwig Jahn, Franz Schuselka, Friedrich Siegmund Jucho, Johann Gustav Moritz Heckscher, Felix Fürst von Lichnowsky, Friedrich Christoph Dahlmann, Anton Ritter von Schmerling, Franz Raveaux, Heinrich Simon, Johann Jacoby, Robert Blum, Johann Erzherzog von Österreich, Louis Cavaignac, Friedrich Hecker, Friedrich von Wrangel, Joseph Wenzel Graf Radetzky, Joseph Freiherr Jellachich von Buszin, Hermann Rollett, Alphonse de Lamartine, Georg Jung, Louis Napoleon, Ernst Alfred Fürst zu Windischgrätz, Ferdinand Freiligrath, Ludwig Kossuth, Emanuel Geibel

Bekanntmachung: Um der Verbreitung unrichtiger Darstellungen über die traurigen Vorfälle vom gestrigen Tage zu begegnen ...

Welsch (Projektbearbeiter): Berlin, 16. Oktober 1848: Aus einer Arbeiterdemonstration entwickeln sich Barrikadenkämpfe mit der Bürgerwehr. Insgesamt 12 Tote, zahlreiche Verwundete

Effects of Prolyl Hydroxylase Inhibitor L-mimosine on Dental Pulp in the Presence of Advanced Glycation End Products

INTRODUCTION Proangiogenic prolyl hydroxylase (PHD) inhibitors represent a novel approach to stimulate tissue regeneration. Diabetes mellitus involves the accumulation of advanced glycation end products (AGEs). Here we evaluated the impact of AGEs on the response of human pulp tissue to the PHD inhibitor L-mimosine (L-MIM) in monolayer cultures of dental pulp-derived cells (DPCs) and tooth slice organ cultures. METHODS In monolayer cultures, DPCs were incubated with L-MIM and AGEs. Viability was assessed based on formazan formation, live-dead staining, annexin V/propidium iodide, and trypan blue exclusion assay. Vascular endothelial growth factor (VEGF), interleukin (IL)-6, and IL-8 production was evaluated by quantitative polymerase chain reaction and immunoassays. Furthermore, expression levels of odontoblast markers were assessed, and alizarin red staining was performed. Tooth slice organ cultures were performed, and VEGF, IL-6, and IL8 levels in their supernatants were measured by immunoassays. Pulp tissue vitality and morphology were assessed by MTT assay and histology. RESULTS In monolayer cultures of DPCs, L-MIM at nontoxic concentrations increased the production of VEGF and IL-8 in the presence of AGEs. Stimulation with L-MIM decreased alkaline phosphatase levels and matrix mineralization also in the presence of AGEs, whereas no significant changes in dentin matrix protein 1 and dentin sialophosphoprotein expression were observed. In tooth slice organ cultures, L-MIM increased VEGF but not IL-6 and IL-8 production in the presence of AGEs. The pulp tissue was vital, and no signs of apoptosis or necrosis were observed. CONCLUSIONS Overall, in the presence of AGEs, L-MIM increases the proangiogenic capacity, but decreases alkaline phosphatase expression and matrix mineralization.

Biochemical MRI predicts hip osteoarthritis in an experimental ovine femoroacetabular impingement model

BACKGROUND Cam-type femoroacetabular impingement (FAI) resulting from an abnormal nonspherical femoral head shape leads to chondrolabral damage and is considered a cause of early osteoarthritis. A previously developed experimental ovine FAI model induces a cam-type impingement that results in localized chondrolabral damage, replicating the patterns found in the human hip. Biochemical MRI modalities such as T2 and T2* may allow for evaluation of the cartilage biochemistry long before cartilage loss occurs and, for that reason, may be a worthwhile avenue of inquiry. QUESTIONS/PURPOSES We asked: (1) Does the histological grading of degenerated cartilage correlate with T2 or T2* values in this ovine FAI model? (2) How accurately can zones of degenerated cartilage be predicted with T2 or T2* MRI in this model? METHODS A cam-type FAI was induced in eight Swiss alpine sheep by performing a closing wedge intertrochanteric varus osteotomy. After ambulation of 10 to 14 weeks, the sheep were euthanized and a 3-T MRI of the hip was performed. T2 and T2* values were measured at six locations on the acetabulum and compared with the histological damage pattern using the Mankin score. This is an established histological scoring system to quantify cartilage degeneration. Both T2 and T2* values are determined by cartilage water content and its collagen fiber network. Of those, the T2* mapping is a more modern sequence with technical advantages (eg, shorter acquisition time). Correlation of the Mankin score and the T2 and T2* values, respectively, was evaluated using the Spearman's rank correlation coefficient. We used a hierarchical cluster analysis to calculate the positive and negative predictive values of T2 and T2* to predict advanced cartilage degeneration (Mankin ≥ 3). RESULTS We found a negative correlation between the Mankin score and both the T2 (p < 0.001, r = -0.79) and T2* values (p < 0.001, r = -0.90). For the T2 MRI technique, we found a positive predictive value of 100% (95% confidence interval [CI], 79%-100%) and a negative predictive value of 84% (95% CI, 67%-95%). For the T2* technique, we found a positive predictive value of 100% (95% CI, 79%-100%) and a negative predictive value of 94% (95% CI, 79%-99%). CONCLUSIONS T2 and T2* MRI modalities can reliably detect early cartilage degeneration in the experimental ovine FAI model. CLINICAL RELEVANCE T2 and T2* MRI modalities have the potential to allow for monitoring the natural course of osteoarthrosis noninvasively and to evaluate the results of surgical treatments targeted to joint preservation.

Eighty percent of patients with surgical hip dislocation for femoroacetabular impingement have a good clinical result without osteoarthritis progression at 10 years

BACKGROUND We previously reported the 5-year followup of hips with femoroacetabular impingement (FAI) that underwent surgical hip dislocation with trimming of the head-neck junction and/or acetabulum including reattachment of the labrum. The goal of this study was to report a concise followup of these patients at a minimum 10 years. QUESTIONS/PURPOSES We asked if these patients had (1) improved hip pain and function; we then determined (2) the 10-year survival rate and (3) calculated factors predicting failure. METHODS Between July 2001 and March 2003, we performed surgical hip dislocation and femoral neck osteoplasty and/or acetabular rim trimming with labral reattachment in 75 patients (97 hips). Of those, 72 patients (93 hips [96%]) were available for followup at a minimum of 10 years (mean, 11 years; range, 10-13 years). We used the anterior impingement test to assess pain and the Merle d'Aubigné-Postel score to assess function. Survivorship calculation was performed using the method of Kaplan and Meier and any of the following factors as a definition of failure: conversion to total hip arthroplasty (THA), radiographic evidence of worsening osteoarthritis (OA), or a Merle d'Aubigné-Postel score less than 15. Predictive factors for any of these failures were calculated using the Cox regression analysis. RESULTS At 10-year followup, the prevalence of a positive impingement test decreased from preoperative 95% to 38% (p < 0.001) and the Merle d'Aubigné-Postel score increased from preoperative 15.3 ± 1.4 (range, 9-17) to 16.9 ± 1.3 (12-18; p < 0.001). Survivorship of these procedures for any of the defined failures was 80% (95% confidence interval, 72%-88%). The strongest predictors of failure were age > 40 years (hazard ratio with 95% confidence interval, 5.9 [4.8-7.1], p = 0.002), body mass index > 30 kg/m(2) (5.5 [3.9-7.2], p = 0.041), a lateral center-edge angle < 22° or > 32° (5.4 [4.2-6.6], p = 0.006), and a posterior acetabular coverage < 34% (4.8 [3.7-5.6], p = 0.006). CONCLUSIONS At 10-year followup, 80% of patients with FAI treated with surgical hip dislocation, osteoplasty, and labral reattachment had not progressed to THA, developed worsening OA, or had a Merle d'Aubigné-Postel score of less than 15. Radiographic predictors for failure were related to over- and undertreatment of acetabular rim trimming.