Quick abnormal-bid-detection method for construction contract auctions

Non-competitive bids have recently become a major concern in both Public and Private sector construction contract auctions. Consequently, several models have been developed to help identify bidders potentially involved in collusive practices. However, most of these models require complex calculations and extensive information that is difficult to obtain. The aim of this paper is to utilize recent developments for detecting abnormal bids in capped auctions (auctions with an upper bid limit set by the auctioner) and extend them to the more conventional uncapped auctions (where no such limits are set). To accomplish this, a new method is developed for estimating the values of bid distribution supports by using the solution to what has become known as the German tank problem. The model is then demonstrated and tested on a sample of real construction bid data and shown to detect cover bids with high accuracy. This work contributes to an improved understanding of abnormal bid behavior as an aid to detecting and monitoring potential collusive bid practices.

Memoirs 1920-1960

Education as artist in "Odenwaldschule" and Oberammergau; return to Berlin and studies in the "Kunstgewerbeschule"; emigration to Paris in 1933; artist circle including Max Ernst; flight to Switzerland in 1942; busts of Pablo Casals and Emil Ludwig; emigration to New York; bust of Bruno Walter.

2nd Arthur Rubinstein Commemorative Medal

Obverse: Portrait of Arthur Rubinstein by Pablo Picasso. Reverse: Inscription

3rd Arthur Rubinstein Commemorative Medal

Obverse: A portrait of Arthur Rubinstein by Pablo Picasso. Reverse: Inscription.

3rd Arthur Rubinstein Commemorative Medal

Obverse: Portrait of Arthur Rubinstein by Pablo Picasso. Reverse: Inscription

2nd Arthur Rubinstein Commemorative Medal

Obverse: Portrait of Arthur Rubinstein by Pablo Picasso. Reverse: Inscription.

A comparative structural bioinformatics analysis of the insulin receptor family ectodomain based on phylogenetic information

The insulin receptor (IR), the insulin-like growth factor 1 receptor (IGF1R) and the insulin receptor-related receptor (IRR) are covalently-linked homodimers made up of several structural domains. The molecular mechanism of ligand binding to the ectodomain of these receptors and the resulting activation of their tyrosine kinase domain is still not well understood. We have carried out an amino acid residue conservation analysis in order to reconstruct the phylogeny of the IR Family. We have confirmed the location of ligand binding site 1 of the IGF1R and IR. Importantly, we have also predicted the likely location of the insulin binding site 2 on the surface of the fibronectin type III domains of the IR. An evolutionary conserved surface on the second leucine-rich domain that may interact with the ligand could not be detected. We suggest a possible mechanical trigger of the activation of the IR that involves a slight ‘twist’ rotation of the last two fibronectin type III domains in order to face the likely location of insulin. Finally, a strong selective pressure was found amongst the IRR orthologous sequences, suggesting that this orphan receptor has a yet unknown physiological role which may be conserved from amphibians to mammals.

Charge transport properties of water dispersible multiwall carbon nanotube-polyaniline composites

The transmission electron microscopy images of in situ prepared multiwall carbon nanotubes (MWNTs)and polyaniline (PANI) composites show that nanotubes are well dispersed in aqueous medium, and the nanofibers of PANI facilitate intertube transport. Although low temperature transport indicates variable range hopping (VRH) mechanism, the dc and ac conductivity become temperature independent as the MWNT content increases. The onset frequency for the increase in conductivity is observed to be strongly dependent on the MWNT weight percent, and the ac conductivity can be scaled onto a master curve. The negative magnetoresistance is attributed to the forward interference scattering mechanism in VRH transport. (C) 2010 American.

How Does the Spacer Length of Cationic Gemini Lipids Influence the Lipoplex Formation with Plasmid DNA? Physicochemical and Biochemical Characterizations and their Relevance in Gene Therapy

Lipoplexes formed by the pEGFP-C3 plasmid DNA (pDNA) and lipid mixtures containing cationic gemini surfactant of the 1,2-bis(hexadecyl dimethyl ammonium) Acmes family referred to as C16CnC16, where n = 2 3, 5, or 12, and the zwitterionic helper lipid, 1,2-dioleoyl-sn-glycero-3-phosphatidylethanolamine (DOPE) have been studied from a wide variety of physical, chemical, and biological standpoints. The study has been carried out using several experimental methods, such as zeta potential, gel electrophoresis, small-angle X-ray scattering (SAXS), cryo-TEM, gene transfection, cell viability/cytotoxicity, and confocal fluorescence microscopy. As reported recently in a communication (J. Am. Chem. Soc. 2011, 133, 18014), the detailed physicochemical and biological studies confirm that, in the presence of the studied series lipid mixtures, plasmid DNA is compacted with a large number of its associated Na+ counterions. This in turn yields a much lower effective negative charge, q(pDNA)(-), a value that has been experimentally obtained for each mixed lipid mixture. Consequently, the cationic lipid (CL) complexes prepared with pDNA and CL/DOPE mixtures to be used in gene transfection require significantly less amount of CL than the one estimated assuming a value of q(DNA)(-) = -2. This drives to a considerably lower cytotoxicity of the gene vector. Depending on the CL molar composition, alpha, of the lipid mixture, and the effective charge ratio of the lipoplex, rho(eff), the reported SAXS data indicate the presence of two or three structures in the same lipoplex, one in the DOPE-rich region, other in the CL-rich region, and another one present at any CL composition. Cryo-TEM and SAXS studies with C16CnC16/DOPE-pDNA lipoplexes indicate that pDNA is localized between the mixed lipid bilayers of lamellar structures within a monolayer of similar to 2 nm. This is consistent with a highly compacted supercoiled pDNA conformation compared with that of linear DNA. Transfection studies were carried out with HEK293T, HeLa, CHO, U343, and H460 cells. The alpha and rho(eff) values for each lipid mixture were optimized on HEK293T cells for transfection, and using these values, the remaining cells were also transfected in absence (-FBS-FBS) and presence (-FBS+FBS) of serum. The transfection efficiency was higher with the CLs of shorter gemini spacers (n = 2 or 3). Each formulation expressed GFP on pDNA transfection and confocal fluorescence microscopy corroborated the results. C16C2C16/DOPE mixtures were the most efficient toward transfection among all the lipid mixtures and, in presence of serum, even better than the Lipofectamine2000, a commercial transfecting agent Each lipid combination was safe and did not show any significant levels of toxicity. Probably, the presence of two coexisting lamellar structures in lipoplexes synergizes the transfection efficiency of the lipid mixtures which are plentiful in the lipoplexes formed by CLs with short spacer (n = 2, 3) than those with the long spacer (n = 5, 12).

Effects of a Delocalizable Cation on the Headgroup of Gemini Lipids on the Lipoplex-Type Nanoaggregates Directly Formed from Plasmid DNA

Lipoplex-type nanoaggregates prepared from pEGFP-C3 plasmid DNA (pDNA) and mixed liposomes, with a gemini cationic lipid (CL) 1,2-bis(hexadecyl imidazolium) alkanes], referred as (C(16)Im)(2)C-n (where C-n is the alkane spacer length, n = 2, 3, 5, or 12, between the imidazolium heads) and DOPE zwitterionic lipid, have been analyzed by zeta potential, gel electrophoresis, SAXS, cryo-TEM, fluorescence anisotropy, transfection efficiency, fluorescence confocal microscopy, and cell viability/cytotoxicity experiments to establish a structure-biological activity relationship. The study, carried out at several mixed liposome compositions, alpha, and effective charge ratios, rho(eff), of the lipoplex, demonstrates that the transfection of pDNA using CLs initially requires the determination of the effective charge of both. The electrochemical study confirms that CLs with a delocalizable positive charge in their headgroups yield an effective positive charge that is 90% of their expected nominal one, while pDNA is compacted yielding an effective negative charge which is only 10-25% than that of the linear DNA. SAXS diffractograms show that lipoplexes formed by CLs with shorter spacer (n = 2, 3, or 5) present three lamellar structures, two of them in coexistence, while those formed by CL with longest spacer (n = 12) present two additional inverted hexagonal structures. Cryo-TEM micrographs show nanoaggregates with two multilamellar structures, a cluster-type (at low alpha value) and a fingerprint-type, that coexist with the cluster-type at moderate alpha composition. The optimized transfection efficiency (TE) of pDNA, in HEK293T, HeLa, and H1299 cells was higher using lipoplexes containing gemini CLs with shorter spacers at low a value. Each lipid formulation did not show any significant levels of toxicity, the reported lipoplexes being adequate DNA vectors for gene therapy and considerably better than both Lipofectamine 2000 and CLs of the 1,2-bis(hexadecyl ammnoniun) alkane series, recently reported.

The nuclease activities of both the Smr domain and an additional LDLK motif are required for an efficient anti-recombination function of Helicobacter pyloriMutS2

Helicobacter pylori, a human pathogen, is a naturally and constitutively competent bacteria, displaying a high rate of intergenomic recombination. While recombination events are essential for evolution and adaptation of H.pylori to dynamic gastric niches and new hosts, such events should be regulated tightly to maintain genomic integrity. Here, we analyze the role of the nuclease activity of MutS2, a protein that limits recombination during transformation in H.pylori. In previously studied MutS2 proteins, the C-terminal Smr domain was mapped as the region responsible for its nuclease activity. We report here that deletion of Smr domain does not completely abolish the nuclease activity of HpMutS2. Using bioinformatics analysis and mutagenesis, we identified an additional and novel nuclease motif (LDLK) at the N-terminus of HpMutS2 unique to Helicobacter and related epsilon-proteobacterial species. A single point mutation (D30A) in the LDLK motif and the deletion of Smr domain resulted in approximate to 5-10-fold loss of DNA cleavage ability of HpMutS2. Interestingly, the mutant forms of HpMutS2 wherein the LDLK motif was mutated or the Smr domain was deleted were unable to complement the hyper-recombination phenotype of a mutS2(-) strain, suggesting that both nuclease sites are indispensable for an efficient anti-recombinase activity of HpMutS2.

Mutations in the nucleotide binding and hydrolysis domains of Helicobacter pylori MutS2 lead to altered biochemical activities and inactivation of its in vivo function

Background: Helicobacter pylori MutS2 (HpMutS2), an inhibitor of recombination during transformation is a non-specific nuclease with two catalytic sites, both of which are essential for its anti-recombinase activity. Although HpMutS2 belongs to a highly conserved family of ABC transporter ATPases, the role of its ATP binding and hydrolysis activities remains elusive. Results: To explore the putative role of ATP binding and hydrolysis activities of HpMutS2 we specifically generated point mutations in the nucleotide-binding Walker-A (HpMutS2-G338R) and hydrolysis Walker-B (HpMutS2-E413A) domains of the protein. Compared to wild-type protein, HpMutS2-G338R exhibited similar to 2.5-fold lower affinity for both ATP and ADP while ATP hydrolysis was reduced by similar to 3-fold. Nucleotide binding efficiencies of HpMutS2-E413A were not significantly altered; however the ATP hydrolysis was reduced by similar to 10-fold. Although mutations in the Walker-A and Walker-B motifs of HpMutS2 only partially reduced its ability to bind and hydrolyze ATP, we demonstrate that these mutants not only exhibited alterations in the conformation, DNA binding and nuclease activities of the protein but failed to complement the hyper-recombinant phenotype displayed by mutS2-disrupted strain of H. pylori. In addition, we show that the nucleotide cofactor modulates the conformation, DNA binding and nuclease activities of HpMutS2. Conclusions: These data describe a strong crosstalk between the ATPase, DNA binding, and nuclease activities of HpMutS2. Furthermore these data show that both, ATP binding and hydrolysis activities of HpMutS2 are essential for the in vivo anti-recombinase function of the protein.

Evaluación de la influencia de cuatro niveles de fertilización completa (12-30-10) en la producción de semillas de Canavalia ensiformis L.

El trabajo se desarrolló de octubre a marzo (1997-1998) en la hacienda Santa Rosa propiedad de la Universidad Nacional Agraria (UNA) en el departamento de Managua. Se evaluó el efecto de cuatro niveles de fertilización completa (12-30-10) y el testigo (sin fertilización) sobre la producción de semillas de Canavalia ensiformis L. Para esto se utilizó un diseño experimental de bloque completo al azar (BCA) y se estudió el efecto de un sólo factor (niveles de fertilización): Con tres repeticiones, formándose un total de 15 parcelas. El ensayo se realizó en un área total de 229.5 m2. Para evaluar las variables: rendimiento de semillas. cantidad promedio de semillas por vainas, largo promedio de vainas, número promedio de vainas por planta y altura de plantas (cm). Teniendo como parcela útil 3 m2. El estudio estadístico contempló el uso de análisis de varianza: El resultado del análisis no mostró diferencia significativa entre bloques ni tratamientos, sin descartar la existencia de diferencias reales. Al realizar el análisis de presupuestos parciales determinamos que el tratamiento de mayor utilidad con respecto al testigo es T2 (24.00 kg/ha) de fertilización 12-30-10. Debemos señalar que este incremento no justifica la aplicación de fertilizante en las condiciones en que se realizó el ensayo ya que la diferencia que se presenta es mínima. La no significancia entre tratamiento se atribuye probablemente al momento de aplicación del fertilizante (al momento de la siembra), que nos hace suponer no fue utilizado por las plantas para la producción de semillas, si no para otras actividades fisiológicas de las mismas.

Utilización de la resina de neem (Azadirachta indica) como desparasitante externo en el tratamiento del tórsalo (Dermatobia hominis) en bovinos del municipio de Muy Muy, departamento de Matagalpa

El presente estudio se realizó con el objetivo de evaluar la utilización de la Resina de Neem (Azadirachta indica)como desparasitante externo en el tratamiento del tórsalo (Dermatobia hominis)en bovinos del Municipio de Muy Muy, Departamento de Matagalpa. El Municipio de Muy Muy esta ubicado en las Coordenadas 12º45'48" de latitud Norte con 85° 37' 36" de Longitud Oeste, con una altura sobre el nivel del mar de 337.6 metros, una precipitación promedio entre 1400-1800 mm, con una temperatura promedio 24°C., La topografía del terreno en que se ubica el Municipio de MUY MUY, presenta las siguientes características: 32.1% Terrenos Plano, 41.0% Terrenos Ondulados y 26.9% Terrenos Quebrados. Y los tipos de suelos son arcillosos y rocosos. En el trabajo experimental se utilizó un diseño completamen te al azar (D.C.A) el que estuvo compuesto por un lote de 18 animales divididos en 3 grupos, cada grupo formado por 6 animales seleccionadas al azar y sometidas a tratamientos distintos Tratamiento I: Ivermectina al 1%, Tratamiento II: Resina del Neem al 5%. Tratamiento III: Resina de Neem al 10%. Para evaluar la efectividad de los distintos tratamientos se hizo conteos periódicos de tórsalos cada siete días, cuantificando la cantidad de nódulos por animal y región anatómica a los 7, 14, 21, 28, 35, 42, 49, 56 días. La efectividad de todos los tratamientos se observó a partir de los 21 días después de la aplicación. Los tratamientos II y I tuvieron las mejores respuestas en el control, del tórsalo, con un porcentaje de efectividad del 91.6%, y 72.7% respectivamente y con un 62.5% para el tratamiento III. El área anatómica mas afectada fue la del miembro anterior derecho seguido del lateral derecho. Según el análisis del costo de la dosis, se puede decir que la resina de Neem es un desparasita nte económico para los productores.