理化因子诱导的CP43和CP47结构与功能变化规律的研究

CP43和CP47是PSII中位于类囊体膜上的两种内周天线色素蛋白复合体，它们都是由六个跨膜的α-螺旋和五个膜外环组成。CP43和CP47的主要功能是把光系统II(PSII)外周天线色素蛋白复合体(LHCII)吸收的能量传给反应中心(RC)，从而引起光化学反应。因此，研究CP43和CP47的结构与功能对于揭示植物光合作用高效吸能、传能的分子机理具有重要意义。由于CP43和CP47的分离纯化比较困难，所以相对于其它的光合膜蛋白来说，人们对CP43和CP47的研究比较少。在本文中，我们在分离、纯化CP43和CP47的基础上，采用多种光谱学和波谱学技术对CP43和CP47在GuHCl和高温作用下的变性过程及其结构与功能的变化规律进行了比较深入的研究，获得了如下结果： 1. CP43和CP47膜外区的结构特点及盐酸胍(GuHCl)引起的变性研究 我们用荧光光谱、园二色(CD)光谱研究了GuHCl引起CP43和CP47的变性过程及其膜外区的结构特点。研究发现：CP43和CP47的膜外区具有一定的有序结构，而不是一种没有规则的伸展状态;和CP43相比，CP47的三级结构及Chl a的微环境对GuHCl更敏感。在GuHCl作用下，从β-Car到Chl a的能量传递变化和三级结构的变化密切相关，而与二级结构变化的相关性则较小;和大多数水溶性蛋白不一样，CP43和CP47对GuHCl变性有一定的抵抗力，而且其变性过程不表现为二态过程，这些都是膜蛋白的特点。 2 CP43和CP47中与芳香族氨基酸有关的能量传递研究 我们用吸收光谱、荧光光谱并参照PSII的3.5 Å的晶体结构分析结果研究了CP43和CP47中与芳香族氨基酸有关的能量传递。发现：和水溶性蛋白不一样，CP43和CP47中的酪氨酸(Tyrs)并不能有效的把其能量传给色氨酸(Trps);CP43和CP47中的芳香族氨基酸能通过Föster机制和Dexter机制把其能量传给Chl a，并且CP47中的传递效率要大于CP43;在CP47中Föster机制是芳香族氨基酸和Chl a之间能量传递的主要方式，而在CP43中Dexter机制则是主要方式。这些结果也暗示了，太阳光中的紫外辐射对植物来说除了其伤害作用以外也有一定的益处。 3 GuHCl诱导CP43和CP47变性的太赫兹（THz）光谱研究 THz时域光谱技术(THz-TDS)是研究分子构型状态的一个新工具。近年来，已被应用于物理或化学分子的研究中。我们首次把这个技术应用到光合膜蛋白CP43和CP47的GuHCl变性研究上。研究发现，在小于1.5 THz时，THz吸收光谱强度随着频率的增加而增加可以看作是蛋白质变性的标志。在GuHCl作用下频域光谱中出现的1.8 THz峰应来源于Chl a和GuHCl之间的相互作用。实验结果表明，THz光谱是区分蛋白分子的不同构型状态以及监测蛋白变性过程的有力工具。 4 CP43热变性的傅立叶变换红外光谱和THz光谱研究 我们用傅立叶变换红外光谱技术(FT-IR)、SDS聚丙稀凝胶电泳(SDS-PAGE)和THz光谱技术对CP43的热变性过程进行了研究。结果表明，在高温处理下，CP43的二级结构发生了变化，且其跃变点发生在59℃。随着温度的逐渐升高，CP43先发生凝集，接着又发生降解;CP43的低频振动模随着温度的升高和分子量的减小也发生变化。我们还证实THz光谱技术在监测膜蛋白的热变性时既有它的优越性，也存在一些不足之处。这些结果为THz-TDS技术在生物样品上的应用提供了基本的资料，并完善了相关的理论。

ABF＿3型复合氟化物的无机合成及稀土掺杂特性研究

本文介绍了钙钛矿型复合氟化物的基本知识和研究进展，并在已有文献的基础上对部分ABF＿3型氟化物的无机合成作了深入研究，进一步讨论了该类化合物的稀土掺杂特性和能量传递现象。首次以条件温和的中温水热法合成了重要激光基质材料KZnF_3、NaZnF_3。产物结构经XRD、SEM、XPS表征，IR和热分析证实水热产物未明显含有分子内结晶水。摸索了最佳合成条件，并比较了与固相合成产物在含氧量上的差异。水热法还实现了Eu的掺杂，荧光光谱和ESR谱均确认Eu进入格位并部分发生价态转变，讨论了Eu~(2+)格位取代和电荷补偿等问题。固相反应合了BaLiF_3:Eu:Gd磷光体多晶粉末。荧光光谱确认体系中Gd~(3+) → Eu~(2+)能量传递的存在。以DEXTER能量传递理论为基础，建立了能量传递模型，计算了能量传递几率，观察到Gd~(3+)和Eu~(2+)之间存在竞争吸收，当Gd~(3+)离子浓度过高，Eu~(2+)发射强度降低。

复合氟化物中Eu（II）和某些稀土离子之间能量传递的研究

掺稀土离子的碱土稀土复合氟化物和ABF_3钙钛矿型氟化物是重要的激光和发光材料。Eu(II)是重要的激活离子。利用Eu(II) d→f跃迁的宽带发射和f→f跃迁的锐线发射，Eu(II)作为短波紫外激光材料手荧光材料的激活剂是优良的候选新。因此，如能找到某种敏化剂对Eu(II)产生能量传递，提高d→f或f→f跃迁发射效率，那么对Eu(II)的潜在应用具有重要的实际和理论意义。人们对Eu(II) d-f、f-f跃迁性度和实现f-f跃迁的条件以及Eu(II)对其它离子的纯量传递已进行了许多深入的研究。然而，人们对其它离子对Eu(II)的纯量传递的研究和报道则甚少。本文从研究Eu(II)和Ce(III)在xMF_2-yYF_3 （M = Ca，Sr，Ba；x = 0，1，2，3；y = 0，1，2，3，4，5）和ABF_3（A = K~+，Ba~(2+)；B = Mg~(2+)，Ca~(2+)，Li~+）两体系中的光谱变经规律入手，比较和分析了Eu(II)和Ce(III)的光谱行为及期 影响因素，特别是仔细地考察了氧对Eu(II)光谱的影响。通过比较和分析，指出Ce(III)对Eu(II)产生能量传递的可能性，寻求两新相互敏化的合适的基质。在复合氟化物体系中首次实现了Ce(III)对Eu(II)的能量传递。根据Dexter能量传递共振理论讨论和分析了Ce(III)对Eu(II)能量传递机理和可能传递途径，提出了能量传递模型，估算了各种传递过程的几率，获取了一些有益启示。同时，对Eu(II)和Tb(III)之间的能量传递也做了初步讨论。与某些基质中Tb(III)可对Ce(III)产生能量传递不同，在讨论体系中没有观察到Tb(III)对Eu(II)的能量传递。

Peasant pioneers; an interpretation of the Slavic peoples in the United States, bx Kenneth D. Miller. Slavic design on cover by B. Mrazek.

http://www.archive.org/details/peasantpioneersa008724mbp

Bringing the gospel in Hogan and Pueblo, by Rev. J. Dolfin.

http://www.archive.org/details/bringinggospelin00dolfrich

IMPROVISATION AND THE CONCERT SAXOPHONIST: A SURVEY OF COMPOSITIONAL AND PERFORMANCE TECHNIQUES

Much of the contemporary concert (i.e. “classical”) saxophone literature has connections to compositional styles found in other genres like jazz, rock, or pop. Although improvisation exists as a dominant compositional device in jazz, improvisation as a performance technique is not confined to a single genre. This study looks at twelve concert saxophone pieces that are grouped into three primary categories of compositional techniques: 1) those containing unmeasured phrases, 2) those containing limited relation to improvisation but a close relationship to jazz styles, and 3) those containing jazz improvisation. In concert saxophone music, specific crossover pieces use the compositional technique of jazz improvisation. Four examples of such jazz works were composed by Dexter Morrill, Phil Woods, Bill Dobbins, and Ramon Ricker, all of which provide a foundation for this study. In addition, pieces containing varying degrees of unmeasured phrases are highlighted. As this dissertation project is based in performance, the twelve pieces were divided into three recitals that summarize a pedagogical sequence. Any concert saxophonist interested in developing jazz improvisational skills can use the pieces in this study as a method to progress toward the performance of pieces that merge jazz improvisation with the concert format. The three compositional techniques examined here will provide the performer with the necessary material to develop this individualized approach to improvisation. Specific compositional and performance techniques vary depending on the stylistic content: this study examines improvisation in the context of concert saxophone repertoire.

The IGNITE network: a model for genomic medicine implementation and research.

BACKGROUND: Patients, clinicians, researchers and payers are seeking to understand the value of using genomic information (as reflected by genotyping, sequencing, family history or other data) to inform clinical decision-making. However, challenges exist to widespread clinical implementation of genomic medicine, a prerequisite for developing evidence of its real-world utility. METHODS: To address these challenges, the National Institutes of Health-funded IGNITE (Implementing GeNomics In pracTicE; www.ignite-genomics.org ) Network, comprised of six projects and a coordinating center, was established in 2013 to support the development, investigation and dissemination of genomic medicine practice models that seamlessly integrate genomic data into the electronic health record and that deploy tools for point of care decision making. IGNITE site projects are aligned in their purpose of testing these models, but individual projects vary in scope and design, including exploring genetic markers for disease risk prediction and prevention, developing tools for using family history data, incorporating pharmacogenomic data into clinical care, refining disease diagnosis using sequence-based mutation discovery, and creating novel educational approaches. RESULTS: This paper describes the IGNITE Network and member projects, including network structure, collaborative initiatives, clinical decision support strategies, methods for return of genomic test results, and educational initiatives for patients and providers. Clinical and outcomes data from individual sites and network-wide projects are anticipated to begin being published over the next few years. CONCLUSIONS: The IGNITE Network is an innovative series of projects and pilot demonstrations aiming to enhance translation of validated actionable genomic information into clinical settings and develop and use measures of outcome in response to genome-based clinical interventions using a pragmatic framework to provide early data and proofs of concept on the utility of these interventions. Through these efforts and collaboration with other stakeholders, IGNITE is poised to have a significant impact on the acceleration of genomic information into medical practice.

Early growth and adult respiratory function in men and women followed from the fetal period to adulthood

While some studies suggest that poor fetal growth rate, as indicated by lower birth weight, is associated with poor respiratory function in childhood, findings among adults remain inconsistent. A study was undertaken to determine the association between early growth and adult respiratory function.

Risk factors, DNA damage, and disease progression in Barrett's esophagus

Esophageal adenocarcinoma develops on a background of Barrett's esophagus. A number of risk factors have been linked to both conditions, including gastroesophageal reflux and smoking. However, the molecular mechanisms by which these factors influence disease progression remain unclear. One possibility is that risk factors generate promutagenic DNA damage in the esophagus. The comet assay was used to measure DNA damage in esophageal (Barrett's and squamous) and gastric mucosa of Barrett's patients with (n = 24) or without (n = 50) associated adenocarcinoma or high-grade dysplasia in comparison with control patients (squamous mucosa) without Barrett's esophagus (n = 64). Patients completed a questionnaire detailing exposure to some of the known risk factors for Barrett's esophagus and adenocarcinoma. In Barrett's esophagus patients, DNA damage was higher in Barrett's mucosa compared with normal esophageal and gastric mucosa (P < 0.001). In addition, the highest quartile of DNA damage in Barrett's mucosa was associated with an increased risk (odds ratio, 9.4; 95% confidence interval, 1.1-83.4; P = 0.044) of developing adenocarcinoma or high-grade dysplasia compared with DNA damage levels in the lowest quartile. Smoking was associated with higher DNA damage in squamous epithelium in all patient groups (P < 0.01) and in Barrett's mucosa (P < 0.05) in Barrett's esophagus patients only. In controls only, current reflux was associated with higher DNA damage, whereas anti-inflammatory drug use resulted in lower levels. Collectively, these data imply a genotoxic insult to the premalignaint Barrett's mucosa that may explain the genetic instability in this tissue and the progression to adenocarcinoma. There is an indication for a role for smoking in inducing DNA damage in esophageal mucosa but an understanding of the role of reflux requires further investigation.

Predictivity Strength of the Spatial Variability of Phenanthrene Sorption Across Two Sandy Loam Fields

Sorption is commonly agreed to be the major process underlying the transport and fate of polycyclic aromatic hydrocarbons (PAHs) in soils. However, there is still a scarcity of studies focusing on spatial variability at the field scale in particular. In order to investigate the variation in the field of phenanthrene sorption, bulk topsoil samples were taken in a 15 × 15-m grid from the plough layer in two sandy loam fields with different texture and organic carbon (OC) contents (140 samples in total). Batch experiments were performed using the adsorption method. Values for the partition coefficient K d (L kg−1) and the organic carbon partition coefficient K OC (L kg−1) agreed with the most frequently used models for PAH partitioning, as OC revealed a higher affinity for sorption. More complex models using different OC compartments, such as non-complexed organic carbon (NCOC) and complexed organic carbon (COC) separately, performed better than single K OC models, particularly for a subset including samples with Dexter n < 10 and OC <0.04 kg kg−1. The selected threshold revealed that K OC-based models proved to be applicable for more organic fields, while two-component models proved to be more accurate for the prediction of K d and retardation factor (R) for less organic soils. Moreover, OC did not fully reflect the changes in phenanthrene retardation in the field with lower OC content (Faardrup). Bulk density and available water content influenced the phenanthrene transport mechanism phenomenon.

Vetus Testamentum cum glossa ordinaria, pars.

Avec arguments. Cant. canticorum V,11-VIII,14 (1) ; Osee I,3-XIV,10 (9) ; Joel I,6-III,21 (38) ; Amos I,1-IX,15 (48) ; Abdias I,7-21 (68) ; Jonas I,1-IV,11 (70v) ; Mich. I,6-VII,20 (77) ; Nahum I,1-III,19 (89v) ; Habacuc I,2-III,19 (95) ; Sophonias I,1-III,20 (102v) ; Agg. I,2-II,24 (110) ; Zachar. I,3-XIV,21 (115) ; Malach. I,4-IV,6 (139).

Morell (André). Correspondance numismatique d'André Morell, d'Arnstatt, pendant les années 1702 et 1703.

Copie du temps comprenant à la fois les lettres de Morell et celles de ses correspondants, savoir : André Fountaine ; — Al. Ferd. comes Marsilii ; — le prince seneschal de Ligne ; — Francesco de Ficoroni ; — Jo. Mich. Dilherrus ; — Jo. Lupt. Marinele Armengol. ; — Doctor J. C. Bon Cretensis ; — Jac. Perizonius ; — Gisb. Cuperus.