Detecção de Histophilus somni (Haemophilus somnus) no sêmen bovino mediante reação em cadeia pela polimerase (PCR)

The present study evaluated the use of PCR for Histophilus somni detection in bovine semen. Semen samples were experimentally infected with H. somni at dilutions ranging from 107 to 101 bacteria/mL and subjected to DNA extraction by the phenol/chloroform method, followed by PCR amplification. The amplification products were analyzed by electrophoresis in 8% acrylamide gel. The oligonucleotide primers used yielded an amplification fragment of 400 base pairs from the bacterial DNA. Positive amplification was obtained even for the 101 bacteria/mL dilution. PCR proved to be an efficient method for the detection of H. somni. The results obtained in this study have brought relevant information for the diagnosis of H. somni, justifying the need for the diagnosis of this bacterium in bulls, especially in semen samples that should be free of contamination. The PCR method has shown to be a useful tool for the quality control of semen produced in artificial insemination centers.

Influência das variações pluviais nos teores de flúor em águas de poços

Introduction: The fluoridation surveillance in the public water supply came up with the need to develop mechanisms that would help society in the control of water quality. To perform reliable analysis, variables such as depth of the wells and pluvial variations should be subject of research and study. Objectives: To analyze the fluoride levels of deep wells in periods of rain and drought, corresponding to four brazilian cities and compare the results. Methodology: It was made the mapping of the water supply network and the identification of the sampling points according to the number of deep wells in each locality, selecting those who had the natural fluoride level. Three points were defined for each source of water supply, and the addresses were randomly selected. The samples were analyzed in duplicate at the laboratory of the Center for Research in Public Health of the Dentistry School of Araçatuba – UNESP, during 8 months, in periods of rain and drought (total absence of rain). Results: 174 analysis were conducted, the mean fluoride levels for rain and drought times were, respectively: 0,71 and 0,73. It was used the Student’s t test (p <0.05). Conclusion: There wasn’t statistically significant differences for the periods of rain and drought in any of the cities, at the year of 2010. This study highlights the importance of health surveillance, because beyond of ensuring social control, there is also the search for investigating variables that may have directly and / or indirectly influence on the results of the analysis.

Validação de protocolo sensorial para avaliação de carne bovina

Beef quality control, particularly its sensory characteristics, is an important factor for producers and retailers in order to satisfy consumer’s choices. Sensory analysis is an important tool to evaluate attributes that cannot be measured by easily available instrumental techniques, as well as texture – tenderness and juiciness – whose human perception is more complete, through trained panels. The aim of this study was evaluate the use of a beef sensory analysis protocol in three different laboratories. Six commercial samples of different brands of aged beef and 14 samples from crossbred animals (Bonsmara × Nelore - 7 and Canchim × Nelore - 7), aged during 14 days were analyzed. The samples were distributed to each participant laboratory, where 7 to 12 panelists were trained. A sheet containing a 9 cm non-structured scale with 14 attributes was used. The attributes were brown colour (CMAR); aponevrosis (PNAP); hydration degree (GH); characteristic beef aroma (SCCB); salty taste (SS); liver flavour (SF); fat flavour (SG); metallic flavour (SM); tenderness (MZ); juiciness (SL); fibrosity (FBS) and liver texture (SF). Obtained data was analyzed using analysis of variance and principal component analysis (PCA). The results showed that there was no interaction between samples and laboratories, indicating that all of them responded in a similar manner in relation to the samples, except PNAP attribute, which was expected as meat is very non-uniform normally. Samples were well differentiated in all laboratories as it could be observed in PCA graphs. With proper training it is possible to use a standard protocol for beef sensory analysis.

Isolamento e identificação das micobactérias em águas tratadas provenientes do sistema de abastecimento de Araraquara-SP

Water quality is very important for the health and the population welfare, and the public supply system must provide water quality and suffi cient quantity for the entire population. Water treatment stations, are the main way to obtain water quality. When this doesn’t occur, several problems can affect the population, in this case, using water with poor quality is a constant risk of emergence causing various diseases. The elimination of microorganisms in treated water reduces competition, encouraging the multiplication of chlorine resistant bacteria as Mycobacterium genus frequently isolated from treated and chlorinated water. Considering the lack of indication from examinations of mycobacteria routine laboratory for quality control of drinking water and other human uses, the objective was to verify the presence isolate and identify the environmental mycobacteria in the system water source surface of Araraquara - SP. We analyzed 40 water samples, distributed as follows: ten water gross collected at Station Water Treatment Plant (WTP), harvested after ten fi ltration; ten collected in the reservoir after chlorination and ten in the network distribution. Were recovered 43 isolates of mycobacteria. All isolates were subjected to PCR-PRA. The mycobacteria were identifi ed as M. lentifl avum, M. parafortuitum, M. genavense, M. gordonae, M. fortuitum, M. confl uent, M. duvalii, M. avium subspecies paratuberculosis and M. szulgai. With these results, was concluded that water is an important source of environmental mycobacteria probably related to several human diseases, suggesting the carrying out continuous monitoring of the microorganisms in the system drinking water.

Bacteriological quality of irrigation water from vegetable gardens in the municipalities of Araraquara, Boa Esperança do Sul and Ibitinga, SP

This work aimed to assess the bacteriological quality of water used in the irrigation of vegetable gardens in the municipalities of Araraquara, Boa Esperança do Sul and Ibitinga, São Paulo, Brazil. A total of 80 samples of water used in the irrigation of 40 vegetable gardens were analyzed, two samples coming from each vegetable garden. They were collected at the same site in different months, which resulted in two sample collection groups. The most probable number (MPN/100mL) of total coliforms, thermotolerant coliforms and enterococci was identifi ed by means of the multiple tube technique. The analysis of the fi rst group samples showed quantities of thermotolerant coliforms above the amount allowed by current law for irrigation water (CONAMA Resolution n. 357) in nine cases. The owners of those vegetable gardens were then instructed in water disinfection procedures. After the analysis of the second group samples, it was noticed that only one sample did not meet the quality standards, and it was collected at a site where no disinfection procedure had been carried out. According to the results, 77.5% of the vegetable gardens were using water whose samples meet the quality standards. After the owners were instructed with regard to disinfection procedures, that number changed to 97.5%, which confi rms the importance of controlling and supervising irrigation water quality

Development and validation of a rapid turbidimetric assay to determine the potency of ampicillin sodium in powder for solution for injection

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Validation of analytical methodology for quantification of cefazolin sodium by liquid chromatography

A reversed-phase high performance liquid chromatography method was validated for the determination of cefazolin sodium in lyophilized powder for solution for injection to be applied for quality control in pharmaceutical industry. The liquid chromatography method was conducted on a Zorbax Eclipse Plus C18 column (250 x 4.6 mm, 5 μm), maintained at room temperature. The mobile phase consisted of purified water: acetonitrile (60: 40 v/v), adjusted to pH 8 with triethylamine. The flow rate was of 0.5 mL min-1 and effluents were monitored at 270 nm. The retention time for cefazolin sodium was 3.6 min. The method proved to be linear (r2 =0.9999) over the concentration range of 30-80 µg mL-1. The selectivity of the method was proven through degradation studies. The method demonstrated satisfactory results for precision, accuracy, limits of detection and quantitation. The robustness of this method was evaluated using the Plackett–Burman fractional factorial experimental design with a matrix of 15 experiments and the statistical treatment proposed by Youden and Steiner. Finally, the proposed method could be also an advantageous option for the analysis of cefazolin sodium, contributing to improve the quality control and to assure the therapeutic efficacy

Estudo comparativo de cápsulas contendo amoxicilina obtidas de algumas farmácias magistrais

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Seleção e associação genômica para precocidade sexual em bovinos da raça Nelore

Pós-graduação em Genética e Melhoramento Animal - FCAV

Desenvolvimento de procedimentos e métodos analíticos no campo forense aplicando os princípios da química verde

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Estudo das interações de eIF5A com a maquinaria de tradução e com o complexo Ribosome Quality Control, utilizando modelo de Saccharomyces cerevisiae

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Estudo genômico do nível de infecção por Babesia bovis em bovinos da raça angus

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Análise de habitações sociais na perspectiva da ergonomia do ambiente construído: estudo de caso do PROMORE

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Alterações celulares induzidas pelo estresse térmico em embriões bovinos

Fundação de Apoio à Pesquisa do Estado de São Paulo (FAPESP)

Estudo de associação genômica e perfil de expressão gênica de folículos antrais em novilhas das raças nelore e angus, associadas ao fenótipo de alta e baixa população folicular

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)