Guía para la discusión de la mesa redonda: planificación estratégica, reglas macrofiscales y descentralización""

Incluye Bibliografía

El cluster lácteo en Chile

Incluye Bibliografía

Las reglas multilaterales sobre subsidios a la luz de algunos fallos de la Organización Mundial del Comercio (OMC)

Incluye Bibliografía

DOCPAL at nineteenth months: an outline of activities, problems and promises (with a complete set of flow diagrams, worksheets, forms, etc)

Includes bibliography

La facilitación del transporte bajo el prisma de las reglas de Rotterdam

El presente documento analiza la importancia de la facilitación del transporte en el desarrollo regional, así como las implicancias de la adopción de las Reglas de Rotterdam para el sector transporte.

Tamoxifen Subcellular Localization; Observation of Cell-Specific Cytotoxicity Enhancement by Inhibition of Mitochondrial ETC Complexes I and III

Recently, a nongenomic cytotoxic component of the chemotherapeutic agent tamoxifen (TAM) has been identified that predominantly triggers mitochondrial events. The present study delineates the intracellular fate of TAM and studies its interaction with a spectrum of cell homeostasis modulators primarily relevant to mitochondria. The subcellular localization of TAM was assessed by confocal fluorescence microscopy. The effect of the modulators on TAM cytotoxicity was assessed by standard MTT assays. Our findings show that in estrogen receptor positive MCF7 breast adenocarcinoma cells and DU145 human prostate cancer cells, TAM largely accumulates in the mitochondria and endoplasmic reticulum, but not lysosomes. Our results further demonstrate that in MCF7, but not in DU145 cells, mitochondrial electron transport chain complex I and III inhibitors exacerbate TAM toxicity with an order of potency of myxothiazol = stigmatellin > rotenone > antimycin A, suggesting a cell-specific cytotoxic interplay between mitochondrial complex I and III function and TAM action.