Vessel orientation-dependent sensitivity of optoacoustic imaging using a linear array transducer

For clinical optoacoustic imaging, linear probes are preferably used because they allow versatile imaging of the human body with real-time display and free-hand probe guidance. The two-dimensional (2-D) optoacoustic image obtained with this type of probe is generally interpreted as a 2-D cross-section of the tissue just as is common in echo ultrasound. We demonstrate in three-dimensional simulations, phantom experiments, and in vivo mouse experiments that for vascular imaging this interpretation is often inaccurate. The cylindrical blood vessels emit anisotropic acoustic transients, which can be sensitively detected only if the direction of acoustic radiation coincides with the probe aperture. Our results reveal for this reason that the signal amplitude of different blood vessels may differ even if the vessels have the same diameter and initial pressure distribution but different orientation relative to the imaging plane. This has important implications for the image interpretation, for the probe guidance technique, and especially in cases when a quantitative reconstruction of the optical tissue properties is required.

Contactless conductivity detector array for capillary electrophoresis.

A CE system featuring an array of 16 contactless conductivity detectors was constructed. The detectors were arranged along 70 cm length of a capillary with 100 cm total length and allow the monitoring of separation processes. As the detectors cannot be accommodated on a conventional commercial instrument, a purpose built set-up employing a sequential injection manifold had to be employed for automation of the fluid handling. Conductivity measurements can be considered universal for electrophoresis and thus any changes in ionic composition can be monitored. The progress of the separation of Na(+) and K(+) is demonstrated. The potential of the system to the study of processes in CZE is shown in two examples. The first demonstrates the differences in the developments of peaks originating from a sample plug with a purely aqueous background to that of a plug containing the analyte ions in the buffer. The second example visualizes the opposite migration of cations and anions from a sample plug that had been placed in the middle of the capillary.

A lung-on-chip array with an integrated bio-inspired respiration mechanism

We report about a lung-on-chip array that mimics the pulmonary parenchymal environment, including the thin, alveolar barrier and the three-dimensional cyclic strain induced by the breathing movements. A micro-diaphragm used to stretch the alveolar barrier is inspired by the in-vivo diaphragm, the main muscle responsible for inspiration. The design of this device aims not only at best reproducing the in-vivo conditions found in the lung parenchyma, but also at making its handling easy and robust. An innovative concept, based on the reversible bonding of the device, is presented that enables to accurately control the concentration of cells cultured on the membrane by easily accessing both sides of the membranes. The functionality of the alveolar barrier could be restored by co-culturing epithelial and endothelial cells that formed tight monolayers on each side of a thin, porous and stretchable membrane. We showed that cyclic stretch significantly affects the permeability properties of epithelial cell layers. Furthermore, we could also demonstrate that the strain influences the metabolic activity and the cytokine secretion of primary human pulmonary alveolar epithelial cells obtained from patients. These results demonstrate the potential of this device and confirm the importance of the mechanical strain induced by the breathing in pulmonary research.

Best Practices in Robot-assisted Radical Cystectomy and Urinary Reconstruction: Recommendations of the Pasadena Consensus Panel

CONTEXT Robot-assisted surgery is increasingly used for radical cystectomy (RC) and urinary reconstruction. Sufficient data have accumulated to allow evidence-based consensus on key issues such as perioperative management, comparative effectiveness on surgical complications, and oncologic short- to midterm outcomes. OBJECTIVE A 2-d conference of experts on RC and urinary reconstruction was organized in Pasadena, California, and the City of Hope Cancer Center in Duarte, California, to systematically review existing peer-reviewed literature on robot-assisted RC (RARC), extended lymphadenectomy, and urinary reconstruction. No commercial support was obtained for the conference. EVIDENCE ACQUISITION A systematic review of the literature was performed in agreement with the PRISMA statement. EVIDENCE SYNTHESIS Systematic literature reviews and individual presentations were discussed, and consensus on all key issues was obtained. Most operative, intermediate-term oncologic, functional, and complication outcomes are similar between open RC (ORC) and RARC. RARC consistently results in less blood loss and a reduced need for transfusion during surgery. RARC generally requires longer operative time than ORC, particularly with intracorporeal reconstruction. Robotic assistance provides ergonomic value for surgeons. Surgeon experience and institutional volume strongly predict favorable outcomes for either open or robotic techniques. CONCLUSIONS RARC appears to be similar to ORC in terms of operative, pathologic, intermediate-term oncologic, complication, and most functional outcomes. RARC consistently results in less blood loss and a reduced need for transfusion during surgery. RARC can be more expensive than ORC, although high procedural volume may attenuate this difference. PATIENT SUMMARY Robot-assisted radical cystectomy (RARC) is an alternative to open surgery for patients with bladder cancer who require removal of their bladder and reconstruction of their urinary tract. RARC appears to be similar to open surgery for most important outcomes such as the rate of complications and intermediate-term cancer-specific survival. Although RARC has some ergonomic advantages for surgeons and may result in less blood loss during surgery, it is more time consuming and may be more expensive than open surgery.

Systematic Review and Cumulative Analysis of Oncologic and Functional Outcomes After Robot-assisted Radical Cystectomy

CONTEXT Although open radical cystectomy (ORC) is still the standard approach, laparoscopic radical cystectomy (LRC) and robot-assisted radical cystectomy (RARC) are increasingly performed. OBJECTIVE To report on a systematic literature review and cumulative analysis of pathologic, oncologic, and functional outcomes of RARC in comparison with ORC and LRC. EVIDENCE ACQUISITION Medline, Scopus, and Web of Science databases were searched using a free-text protocol including the terms robot-assisted radical cystectomy or da Vinci radical cystectomy or robot* radical cystectomy. RARC case series and studies comparing RARC with either ORC or LRC were collected. A cumulative analysis was conducted. EVIDENCE SYNTHESIS The searches retrieved 105 papers, 87 of which reported on pathologic, oncologic, or functional outcomes. Most series were retrospective and had small case numbers, short follow-up, and potential patient selection bias. The lymph node yield during lymph node dissection was 19 (range: 3-55), with half of the series following an extended template (yield range: 11-55). The lymph node-positive rate was 22%. The performance of lymphadenectomy was correlated with surgeon and institutional volume. Cumulative analyses showed no significant difference in lymph node yield between RARC and ORC. Positive surgical margin (PSM) rates were 5.6% (1-1.5% in pT2 disease and 0-25% in pT3 and higher disease). PSM rates did not appear to decrease with sequential case numbers. Cumulative analyses showed no significant difference in rates of surgical margins between RARC and ORC or RARC and LRC. Neoadjuvant chemotherapy use ranged from 0% to 31%, with adjuvant chemotherapy used in 4-29% of patients. Only six series reported a mean follow-up of >36 mo. Three-year disease-free survival (DFS), cancer-specific survival (CSS), and overall survival (OS) rates were 67-76%, 68-83%, and 61-80%, respectively. The 5-yr DFS, CSS, and OS rates were 53-74%, 66-80%, and 39-66%, respectively. Similar to ORC, disease of higher pathologic stage or evidence of lymph node involvement was associated with worse survival. Very limited data were available with respect to functional outcomes. The 12-mo continence rates with continent diversion were 83-100% in men for daytime continence and 66-76% for nighttime continence. In one series, potency was recovered in 63% of patients who were evaluable at 12 mo. CONCLUSIONS Oncologic and functional data from RARC remain immature, and longer-term prospective studies are needed. Cumulative analyses demonstrated that lymph node yields and PSM rates were similar between RARC and ORC. Conclusive long-term survival outcomes for RARC were limited, although oncologic outcomes up to 5 yr were similar to those reported for ORC. PATIENT SUMMARY Although open radical cystectomy (RC) is still regarded as the standard treatment for muscle-invasive bladder cancer, laparoscopic and robot-assisted RCs are becoming more popular. Templates of lymph node dissection, lymph node yields, and positive surgical margin rates are acceptable with robot-assisted RC. Although definitive comparisons with open RC with respect to oncologic or functional outcomes are lacking, early results appear comparable.

Außerordentliches Extrablatt: Die erste goldene Frucht des Reichstages! Kein Robot! Kein Zehnt mehr! Die Stunde der Befreiung hat geschlagen, das schreckliche Wort "Unterthan" ist aus der deutschen Sprache verschwunden ...

Welsch (Projektbearbeiter): Vollendung der Bauernbefreiung, nachdem die Leibeigenschaft bereits 1781/82 unter Kaiser Joseph II. aufgehoben wurde: Bekanntgabe der Aufhebung der obrigkeitlichen Gewalt der Grundherren (ohne Entgelt) sowie der Aufhebung der Dienste und Abgaben (gegen Entschädigung). (Einstimmiger Beschluß des österreichischen Reichstages nach dem diesbezüglichen Antrag des Abgeordneten Hans Kudlich vom 26. Juli 1848)

Der Bauer ist frei: Kein Zehent, keine Robot mehr, beschlossen und mit allgemeine [sic!] Stimmenmehrheit angenommen durch die hohe Reichsversammlung, am 30. und 31. August 1848: Heute endlich gelangte der hohe Reichstag dahin, über des Abgeordneten Lasser's Antrag abzustimmen ...

Welsch (Projektbearbeiter): Der Kudlichsche Antrag auf Bauernbefreiung (26. Juli 1848) wird mit einigen Modifikationen (Abgeordneter Lasser) vom österreichischen Reichstag angenommen. Nebst Abdruck von Redebeiträgen aus den Vordebatten

Die letzte Robot: Die Bauern: "Der Wagen ist schwer, aber wir wollen hoffen ..."

Welsch (Projektbearbeiter): Satirische Kommentierung der Amendements zum Kudlichschen Antrag über die Aufhebung der bäuerlichen Untertänigkeit, die Entschädigungsfrage betreffend

Des Kaisers Gespräch in Schönbrunn mit dem Bauer Nikolaus B... aus G... : der ihm über das gewordene Geschenk der Robot- und der Zehent-Aufhebung dankend zu den Füßen fallen wollte

P. Pichler

In-vivo diffusion tensor imaging of rat spinal cord with a phased array coil at 7T

Magnetic resonance imaging (MRI) is a non-invasive technique that offers excellent soft tissue contrast for characterizing soft tissue pathologies. Diffusion tensor imaging (DTI) is an MRI technique that has shown to have the sensitivity to detect subtle pathology that is not evident on conventional MRI. ^ Rats are commonly used as animal models in characterizing the spinal cord pathologies including spinal cord injury (SCI), cancer, multiple sclerosis, etc. These pathologies could affect both thoracic and cervical regions and complete characterization of these pathologies using MRI requires DTI characterization in both the thoracic and cervical regions. Prior to the application of DTI for investigating the pathologic changes in the spinal cord, it is essential to establish DTI metrics in normal animals. ^ To date, in-vivo DTI studies of rat spinal cord have used implantable coils for high signal-to-noise ratio (SNR) and spin-echo pulse sequences for reduced geometric distortions. Implantable coils have several disadvantages including: (1) the invasive nature of implantation, (2) loss of SNR due to frequency shift with time in the longitudinal studies, and (3) difficulty in imaging the cervical region. While echo planar imaging (EPI) offers much shorter acquisition times compared to spin-echo imaging, EPI is very sensitive to static magnetic field inhomogeneities and the existing shimming techniques implemented on the MRI scanner do not perform well on spinal cord because of its geometry. ^ In this work, an integrated approach has been implemented for in-vivo DTI characterization of rat spinal cord in the thoracic and cervical regions. A three element phased array coil was developed for improved SNR and extended spatial coverage. A field-map shimming technique was developed for minimizing the geometric distortions in EPI images. Using these techniques, EPI based DWI images were acquired with optimized diffusion encoding scheme from 6 normal rats and the DTI-derived metrics were quantified. ^ The phantom studies indicated higher SNR and smaller bias in the estimated DTI metrics than the previous studies in the cervical region. In-vivo results indicated no statistical difference in the DTI characteristics of either gray matter or white matter between the thoracic and cervical regions. ^

Historia de los medios de comunicación de Misiones: Los inicios de la TV por cable. El caso de Canal 2 de Posadas. 16H340

Misiones, es una provincia pequeña, fronteriza y multicultural1, que se caracteriza por contar -a lo largo de su historia- con una variada y rica presencia de medios de comunicación. Desde el origen de los primeros parapetos (1872), pasando por los primeros diarios (1883), la aparición de la radio (1927) hasta llegar a la TV abierta (1972), existen una serie de emprendimientos comunicacionales llevados adelante con éxito por verdaderos pioneros en la materia. Es el caso de la televisión por cable, cuya existencia data desde 1964 en la ciudad de Posadas. Un medio pequeño que llegó a tener en su época de esplendor cerca de 4.000 usuarios. Esto nos permite pensar que más allá de los diversos contextos y momentos histórico, el ciudadano necesitó comunicar y comunicarse entre si y con los demás. El presente proyecto, es la continuidad de un trabajo de investigación que comenzó con la construcción de la historia de LT 85 TV Canal 12 de Posadas, que tiene por objetivo rescatar la memoria de los medios de comunicación de la Provincia de Misiones, en este caso, de Canal 2 de Posadas. Se intentará explorar los modos en que se creó, construyó y usó esta tecnología y los modos en que operó en prácticas y actos comunicativos de unos y otros, en espacios y tiempos múltiples.

Historia de los medios de comunicación de Misiones: Los inicios de la TV por cable. El caso de Canal 2 de Posadas. 16H340

Misiones, es una provincia pequeña, fronteriza y multicultural que se caracteriza por contar -a lo largo de su historia- con una variada y rica presencia de medios de comunicación. Desde el origen de los primeros parapetos (1872), pasando por los primeros diarios (1883), la aparición de la radio (1927) hasta llegar a la TV abierta (1972), existen una serie de emprendimientos comunicacionales llevados adelante con éxito por verdaderos pioneros en la materia. Es el caso de la televisión por cable, cuya existencia data desde 1965 en la ciudad de Posadas. Un medio pequeño que llegó a tener en su época de esplendor cerca de 4.000 usuarios. Esto nos permite pensar que más allá de los diversos contextos y momentos históricos, el ciudadano necesitó comunicar y comunicarse entre sí y con los demás. El proyecto es la continuidad de un trabajo de investigación que comenzó con la construcción de la historia de LT 85 TV Canal 12 de Posadas y, tiene por objetivo rescatar la memoria de los medios de comunicación de la Provincia de Misiones, en este caso, Canal 2 de Posadas. Se intentará explorar los modos en que se creó, construyó y usó esta tecnología y los modos en que operó en prácticas y actos comunicativos de unos y otros, en espacios y tiempos múltiples.

Description of manganese nodules collected by the U.S.S. NERO during survey for a trans-pacific cable

In the early part of 1899 the U.S.S. Nero was dispatched from San Francisco to survey a route for a telegraph cable between the United States, the Philippines Islands and Japan. Concurent with meteorological and oceanographic observations, closely spaced samples of bottom material were systematically sampled. They have been carefully accounted and described by James M. Flint in this volume. On the way, numerous submarine peaks were discovered. During this voyage U.S.S. Nero also took a sounding in the area of the Challenger Deep, recording a depth of 5269 fathoms (9636 m), the greatest depth recorded at that time. Carefull study of the deep-sea deposits have also revealed a number of manganese nodules and encrustations as well as micronodules.