Oxygen, carbon and hydrogen isotope studies of contact metamorphism

The O¹⁸/O¹⁶, C¹³/C¹², and D/H ratios have been determined for rocks and coexisting minerals from several granitic plutons and their contact metamorphic aureoles in northern Nevada, eastern California, central Colorado, and Texas, with emphasis on oxygen isotopes. A consistent order of O¹⁸/O¹⁶, C¹³/C¹², and D/H enrichment in coexisting minerals, and a correlation between isotopic fractionations among coexisting mineral pairs are in general observed, suggesting that mineral assemblages tend to approach isotopic equilibrium during contact metamorphism. In certain cases, a correlation is observed between oxygen isotopic fractionations of a mineral pair and sample distance from intrusive contacts. Isotopic temperatures generally show good agreement with heat flow considerations. Based on the experimentally determined quartz-muscovite O¹⁸/O¹⁶ fractionation calibration curve, temperatures are estimated to be 525 to 625°C at the contacts of the granitic stocks studied.

Small-scale oxygen isotope exchange effects between intrusive and country rock are observed over distances of 0.5 to 3 feet on both sides of the contacts; the isotopic gradients are typically 2 to 3 per mil per foot. The degree of oxygen isotopic exchange is essentially identical for different coexisting minerals. This presumably occurred through a diffusion-controlled recrystallization process. The size of the oxygen isotope equilibrium systems in the small-scale exchanged zones vary from about 1.5 cm to 30 cm. A xenolith and a re-entrant of country rock projecting into on intrusive hove both undergone much more extensive isotopic exchange (to hundreds of feet); they also show abnormally high isotopic temperatures. The marginal portions of most plutons have unusually high O¹⁸/O¹⁶ ratios compared to "normal" igneous rocks, presumably due to large-scale isotopic exchange with meta-sedimentary country rocks when the igneous rocks were essentially in a molten state. The isotopic data suggest that outward horizontal movement of H₂O into the contact metamorphic aureoles is almost negligible, but upward movement of H₂O may be important. Also, direct influx and absorption of water from the country rock may be significant in certain intrusive stocks.

Except in the exchanged zones, the O¹⁸/O¹⁶ ratios of pelitic rocks do not change appreciably during contact metamorphism, even in the cordierite and sillimanite grades; this is in contrast to regional metamorphic rocks which commonly decrease in O¹⁸ with increasing grade. Low O¹⁸/O¹⁶ and C¹³/C¹² ratios of the contact metamorphic marbles generally correlate well with the presence of calc-silicate minerals, indicating that the CO₂ liberated during metamorphic decarbonation reactions is enriched in both O¹⁸ and C¹³ relative to the carbonates.

The D/H ratios of biotites in the contact metamorphic rocks and their associated intrusions show a geographic correlation that is similar to that shown by the D/H ratios of meteoric surface waters, perhaps indicating that meteoric waters were present in the rocks during crystallization of the biotites.

Metasomatism and magmatic assimilation at a gabbro-limestone contact, Christmas Mountains, Big Bend region, Texas

A composite stock of alkaline gabbro and syenite is intrusive into limestone of the Del Carmen, Sue Peake and Santa Elena Formations at the northwest end of the Christmas Mountains. There is abundant evidence of solution of wallrock by magma but nowhere are gabbro and limestone in direct contact. The sequence of lithologies developed across the intrusive contact and across xenoliths is gabbro, pyroxenite, calc-silicate skarn, marble. Pyroxenite is made up of euhedral crystals of titanaugite and sphene in a leucocratic matrix of nepheline, Wollastonite and alkali feldspar. The uneven modal distribution of phases in pyroxenite and the occurrence' of nepheline syenite dikes, intrusive into pyroxenite and skarn, suggest that pyroxenite represents an accumulation of clinopyroxene "cemented" together by late-solidifying residual magma of nepheline syenite composition. Assimilation of limestone by gabbroic magma involves reactions between calcite and magma and/or crystals in equilibrium with magma and crystallization of phases in which the magma is saturated, to supply energy for the solution reaction. Gabbroic magma was saturated with plagioclase and clinopyroxene at the time of emplacement. The textural and mineralogic features of pyroxenite can be produced by the reaction 2( 1-X) CALCITE + AN_XAB_l-X = (1-X) NEPHELINE+ 2(1-X) WOLLASTONITE+ X ANORTHITE+ 2(1-X) CO₂. Plagioclase in pyroxenite has corroded margins and is rimmed by nepheline, suggestive of resorption by magma. Anorthite and wollastonite enter solid solution in titanaugite. For each mole of calcite dissolved, approximately one mole of clinopyroxene was crystallized. Thus the amount of limestone that may be assimilated is limited by the concentration of potential clinopyroxene in the magma. Wollastonite appears as a phase when magma has been depleted in iron and magnesium by crystallization of titanaugite. The predominance of mafic and ultramafic compositions among contaminated rocks and their restriction to a narrow zone along the intrusive contact provides little evidence for the generation of a significant volume of desilicated magma as a result of limestone assimilation.

Within 60 m of the intrusive contact with the gabbro, nodular chert in the Santa Elena Limestone reacted with the enveloping marble to form spherical nodules of high-temperature calc-silicate minerals. The phases wollastonite, rankinite, spurrite, tilleyite and calcite, form a series of sharply-bounded, concentric monomineralic and two-phase shells which record a step-wise decrease in silica content from the core of a nodule to its rim. Mineral zones in the nodules vary 'with distance from the gabbro as follows:

0-5 m CALCITE + SPURRITE + RANKINITE + WOLLASTONITE
5-16 m CALCITE + TILLEYITE ± SPURRITE + RANKINITE + WOLLASTONITE
16-31 m CALCITE + TILLEYITE + WOLLASTONITE
31-60 m CALCITE + WOLLASTONITE
60-plus CALCITE + QUARTZ

The mineral of a one-phase zone is compatible with the phases bounding it on either side but these phases are incompatible in the same volume of P-T-X_CO2.

Growth of a monomineralio zone is initiated by reaction between minerals of adjacent one-phase zones which become unstable with rising temperature to form a thin layer of a new single phase that separates the reactants and is compatible with both of them. Because the mineral of the new zone is in equilibrium with the phases at both of its contacts, gradients in the chemical potentials of the exchangeable components are established across it. Although zone boundaries mark discontinuities in the gradients of bulk composition, two-phase equilibria at the contacts demonstrate that the chemical potentials are continuous. Hence, Ca, Si and CO₂ were redistributed in the growing nodule by diffusion. A monomineralic zone grows at the expense of an adjacent zone by reaction between diffusing components and the mineral of the adjacent zone. Equilibria between two phases at zone boundaries buffers the chemical potentials of the diffusing species. Thus, within a monomineralic zone, the chemical potentials of the diffusing components are controlled external to the local assemblage by the two-phase equilibria at the zone boundaries.

Mineralogically zoned calc-silicate skarn occurs as a narrow band that separates pyroxenite and marble along the intrusive contact and forms a rim on marble xenoliths in gabbro. Skarn consists of melilite or idocrase pseudomorphs of melili te, one or two . stoichiometric calcsilicate phases and accessory Ti-Zr garnet, perovskite and magnetite. The sequence of mineral zones from pyroxenite to marble, defined by a characteristic calc-silicate, is wollastonite, rankinite, spurrite, calcite. Mineral assemblages of adjacent skarn zones are compatible and the set of zones in a skarn band defines a facies type, indicating that the different mineral assemblages represent different bulk compositions recrystallized under identical conditions. The number of phases in each zone is less than the number that might be expected to result from metamorphism of a general bulk composition under conditions of equilibrium, trivariant in P, T and u_CO2. The "special" bulk composition of each zone is controlled by reaction between phases of the zones bounding it on either side. The continuity of the gradients of composition of melilite and garnet solid solutions across the skarn is consistent with the local equilibrium hypothesis and verifies that diffusion was the mechanism of mass transport. The formula proportions of Ti and Zr in garnet from skarn vary antithetically with that of Si Which systematically decreases from pyroxenite to marble. The chemical potential of Si in each skarn zone was controlled by the coexisting stoichiometric calc-silicate phases in the assemblage. Thus the formula proportion of Si in garnet is a direct measure of the chemical potential of Si from point to point in skarn. Reaction between gabbroic magma saturated with plagioclase and clinopyroxene produced nepheline pyroxenite and melilite-wollastonite skarn. The calcsilicate zones result from reaction between calcite and wollastonite to form spurrite and rankinite.

Comunidades de anfíbios anuros em duas fitofisionomias do Parque Estadual da Serra do Tabuleiro, estado de Santa Catarina

O estado de Santa Catarina possuía sua área inteiramente coberta por Mata Atlântica, um dos biomas mais ameaçados mundialmente. Mesmo com o crescente aumento de informações sobre os anfíbios no estado, ainda existe muitas lacunas de informações para este grupo e o desconhecimento é alto mesmo no interior das Unidades de Conservação. O Parque Estadual da Serra do Tabuleiro é a maior unidade de conservação de proteção integral de Santa Catarina e ainda assim possui inúmeras ameaças e pressões antrópicas. Os objetivos deste estudo foram estudar os anfíbios anuros em duas fitofisionomias, restinga e floresta ombrófila densa submontana, do Parque Estadual da Serra do Tabuleiro, verificar as diferenças dos parâmetros da comunidade entre três mesohábitats na restinga, compreender em que extensão algumas variáveis estruturais do hábitat afetam a riqueza e abundância dos anuros na restinga e na floresta ombrófila densa submontana, conhecer os anuros de folhiço na área de floresta e, adicionalmente, verificar o atual conhecimento sobre os anuros de folhiço no mundo. Os anuros no parque foram amostrados por meio dos métodos de procura ativa em transecções, armadilhas de queda com cerca guia e parcelas no chão da floresta durante três anos consecutivos com amostragens trimestrais, de julho de 2007 a abril de 2010. As variáves estruturais foram medidas nos locais amostrados enquanto que os dados climáticos foram obtidos da estação meteorológica mais próxima da área de estudo. A revisão sobre os anuros de folhiço no mundo foi realizada por uma busca na base de dados ISI Web if Science entre os anos de 1945 e 2008. Foram registradas 39 espécies de anuros no Parque, sendo 15 espécies para a restinga, 31 espécies na floresta. No folhiço do chão da floresta, a comunidade foi composta por 13 espécies de anuros. No total, 66% dos anuros registrados aqui, foram endêmicos da Mata Atlântica, 17% possuíram distribuição restrita à Santa Catarina e quatro espécies foram consideradas como vulneráveis na lista de espécies ameaçadas de extinção de Santa Catarina. Na restinga, a área aberta e a mata de restinga foram as mais dissimilares com relação à composição e abundância de anuros e também para as variáveis estruturais do hábitat. Na floresta ombrófila densa, a cobertura de dossel e o número de corpos dágua foram as variáveis mais importantes para a riqueza e abundância das espécies de anuros. Também para os anuros de folhiço do parque, a cobertura de dossel foi importante, conjuntamente com a umidade do ar e a profundidade de folhiço. O atual conhecimento sobre os anuros de folhiço no mundo se concentra nas regiões tropicais, sendo que o Brasil possui o maior número de estudos. Algumas tendências para as comunidades de anuros de folhiço puderam ser identificadas, mas estudos adicionais são necessários para que mais inferências possam ser feitas. O presente estudo contribuiu para preencher parte das lacunas de conhecimento existentes para a biodiversidade dos anuros do estado de Santa Catarina e em especial para o Parque Estadual da Serra do Tabuleiro.

A GEOPASS-NERC project on diatom deposition and sediment accumulation in Lake Baikal, Siberia

This new project is multidisciplinary, with physical and chemical palaeolimnological aspects mainly the responsibility of Swiss and Russian scientists, and the biological limnology and palaeolimnology components mainly undertaken by the British and Russian groups. The overall project aim is to improve palaeoclimate reconstructions using sedimentary diatoms by promoting better understanding of diatom ecology and sediment-forming processes. The initial work plan is divided into four main parts: To understand diatom phytoplankton ecology more fully, to assess taphonomic changes associated with the transformation of phytoplankton diatom communities into sediment assemblages, to demonstrate sediment core integrity and representativity and to calibrate modern diatom assemblages against contemporary climate records. The preliminary results from the interrelated studies of phytoplankton, sediment traps and sediment cores used in GEOPASS-NERC, demonstrate the complexity of links between the living and fossil systems. Furthermore, the nature of recent sedimentation in Lake Baikal is spatially variable and incompletely known. This poses a major challenge to palaeolimnological interpretation. Turbidite deposits and differential preservation of microfossils, combined with inadequate knowledge of the modern ecology of endemic diatoms, all conspire to obfuscate the sedimentary record of environmental change.

Environmental variables and fisheries diversity of the Naaf River Estuary, Bangladesh

The Naaf River estuary is one of the large estuaries in the Bangladesh coastal region not to have been affected by extensive human disturbance. This research provides information about the fisheries diversity status by Estuarine Set Bag Net (ESBN) sampling relation to physicochemical variables in both spatio-temporal scales. About 25 km of the lower estuary was divided into six zones for sample collection by considering the accessibility and availability of the ESBN operation, fish landing centers and location of the fishing villages. In total 48 samples have been analyzed which were taken throughout March to October 2006. To quantify the species diversity, all fisheries data were analyzed by using EstimateS and EcoSim software which accounts the different diversity indices viz., species richness, Shannon–Wiener diversity Index, Dominance and Evenness index. The research results demonstrate that the Naaf River estuary is a habitat of 161 (species richness, Sobs=161, Choa 1=162±2.34, ACE=161.73) different species which belong to 98 fin fishes, 23 shrimps and prawns,13 crabs, 11 molluscs, 3 echinoderms, 4 other crustaceans;while 9 remain unidentified. Results on the aquatic environment,mainly salinity and turbidity were found to have a major influence on their occurrence and distribution. All the findings indicated that the Naaf River estuary is a highly productive system and provides a favourable environment for large variety of estuarine species assemblages.

Geochemistry and resonance ionization of platinum-group elements

Experimental studies were conducted with the goals of 1) determining the origin of Pt- group element (PGE) alloys and associated mineral assemblages in refractory inclusions from meteorites and 2) developing a new ultrasensitive method for the in situ chemical and isotopic analysis of PGE. A general review of the geochemistry and cosmochemistry of the PGE is given, and specific research contributions are presented within the context of this broad framework.

An important step toward understanding the cosmochemistry of the PGE is the determination of the origin of POE-rich metallic phases (most commonly εRu-Fe) that are found in Ca, AJ-rich refractory inclusions (CAI) in C3V meteorites. These metals occur along with γNi-Fe metals, Ni-Fe sulfides and Fe oxides in multiphase opaque assemblages. Laboratory experiments were used to show that the mineral assemblages and textures observed in opaque assemblages could be produced by sulfidation and oxidation of once homogeneous Ni-Fe-PGE metals. Phase equilibria, partitioning and diffusion kinetics were studied in the Ni-Fe-Ru system in order to quantify the conditions of opaque assemblage formation. Phase boundaries and tie lines in the Ni-Fe-Ru system were determined at 1273, 1073 and 873K using an experimental technique that allowed the investigation of a large portion of the Ni-Fe-Ru system with a single experiment at each temperature by establishing a concentration gradient within which local equilibrium between coexisting phases was maintained. A wide miscibility gap was found to be present at each temperature, separating a hexagonal close-packed εRu-Fe phase from a face-centered cubic γNi-Fe phase. Phase equilibria determined here for the Ni-Fe-Ru system, and phase equilibria from the literature for the Ni-Fe-S and Ni-Fe-O systems, were compared with analyses of minerals from opaque assemblages to estimate the temperature and chemical conditions of opaque assemblage formation. It was determined that opaque assemblages equilibrated at a temperature of ~770K, a sulfur fugacity 10 times higher than an equilibrium solar gas, and an oxygen fugacity 10⁶ times higher than an equilibrium solar gas.

Diffusion rates between -γNi-Fe and εRu-Fe metal play a critical role in determining the time (with respect to CAI petrogenesis) and duration of the opaque assemblage equilibration process. The diffusion coefficient for Ru in Ni (D^Ru_Ni) was determined as an analog for the Ni-Fe-Ru system by the thin-film diffusion method in the temperature range of 1073 to 1673K and is given by the expression:

D^Ru_Ni (cm² sec^-1) = 5.0(±0.7) x 10^-3 exp(-2.3(±0.1) x 10¹² erg mole^-1/RT) where R is the gas constant and T is the temperature in K. Based on the rates of dissolution and exsolution of metallic phases in the Ni-Fe-Ru system it is suggested that opaque assemblages equilibrated after the melting and crystallization of host CAI during a metamorphic event of ≥ 10³ years duration. It is inferred that opaque assemblages originated as immiscible metallic liquid droplets in the CAI silicate liquid. The bulk compositions of PGE in these precursor alloys reflects an early stage of condensation from the solar nebula and the partitioning of V between the precursor alloys and CAI silicate liquid reflects the reducing nebular conditions under which CAI were melted. The individual mineral phases now observed in opaque assemblages do not preserve an independent history prior to CAI melting and crystallization, but instead provide important information on the post-accretionary history of C3V meteorites and allow the quantification of the temperature, sulfur fugacity and oxygen fugacity of cooling planetary environments. This contrasts with previous models that called upon the formation of opaque assemblages by aggregation of phases that formed independently under highly variable conditions in the solar nebula prior to the crystallization of CAI.

Analytical studies were carried out on PGE-rich phases from meteorites and the products of synthetic experiments using traditional electron microprobe x-ray analytical techniques. The concentrations of PGE in common minerals from meteorites and terrestrial rocks are far below the ~100 ppm detection limit of the electron microprobe. This has limited the scope of analytical studies to the very few cases where PGE are unusually enriched. To study the distribution of PGE in common minerals will require an in situ analytical technique with much lower detection limits than any methods currently in use. To overcome this limitation, resonance ionization of sputtered atoms was investigated for use as an ultrasensitive in situ analytical technique for the analysis of PGE. The mass spectrometric analysis of Os and Re was investigated using a pulsed primary Ar⁺ ion beam to provide sputtered atoms for resonance ionization mass spectrometry. An ionization scheme for Os that utilizes three resonant energy levels (including an autoionizing energy level) was investigated and found to have superior sensitivity and selectivity compared to nonresonant and one and two energy level resonant ionization schemes. An elemental selectivity for Os over Re of ≥ 10³ was demonstrated. It was found that detuning the ionizing laser from the autoionizing energy level to an arbitrary region in the ionization continuum resulted in a five-fold decrease in signal intensity and a ten-fold decrease in elemental selectivity. Osmium concentrations in synthetic metals and iron meteorites were measured to demonstrate the analytical capabilities of the technique. A linear correlation between Os⁺ signal intensity and the known Os concentration was observed over a range of nearly 10⁴ in Os concentration with an accuracy of ~ ±10%, a millimum detection limit of 7 parts per billion atomic, and a useful yield of 1%. Resonance ionization of sputtered atoms samples the dominant neutral-fraction of sputtered atoms and utilizes multiphoton resonance ionization to achieve high sensitivity and to eliminate atomic and molecular interferences. Matrix effects should be small compared to secondary ion mass spectrometry because ionization occurs in the gas phase and is largely independent of the physical properties of the matrix material. Resonance ionization of sputtered atoms can be applied to in situ chemical analysis of most high ionization potential elements (including all of the PGE) in a wide range of natural and synthetic materials. The high useful yield and elemental selectivity of this method should eventually allow the in situ measurement of Os isotope ratios in some natural samples and in sample extracts enriched in PGE by fire assay fusion.

Phase equilibria and diffusion experiments have provided the basis for a reinterpretation of the origin of opaque assemblages in CAI and have yielded quantitative information on conditions in the primitive solar nebula and cooling planetary environments. Development of the method of resonance ionization of sputtered atoms for the analysis of Os has shown that this technique has wide applications in geochemistry and will for the first time allow in situ studies of the distribution of PGE at the low concentration levels at which they occur in common minerals.

History, origins and importance of temporary ponds

In Europe, temporary ponds are a naturally common and widespread habitat occurring, often in abundance, in all biogeographical regions from the boreal snow-melt pools of northern Scandinavia to the seasonally inundated coastal dune pools of southern Spain. Ecological studies in Europe and elsewhere also emphasise that temporary ponds are a biologically important habitat type, renowned both for their specialised assemblages and the considerable numbers of rare and endemic species they support. They are, however, a habitat currently under considerable threat. Most temporary ponds are inherently shallow and the majority are destroyed even by limited soil drainage for agriculture or urban development. The paper gives an overview of definitions of temporary ponds and examines their formation and abundance. The authors also summarise a visit to the Bialowieza Forest in Poland to investigate the occurrence of temporary ponds.

Oxygen isotope ratios in coexisting minerals of regionally metamorphosed rocks

The O¹⁸/O¹⁶ ratios of coexisting minerals from a number of regionally metamorphosed rocks have been measured, using a bromine pentafluoride extraction-technique. Listed in order of their increasing tendency to concentrate O¹⁸, the minerals analyzed are magnetite, ilmenite, chlorite, biotite, garnet, hornblende, kyanite, muscovite, feldspar, and quartz. The only anomalous sequence detected occurs in a xenolith of schist, in which quartz, muscovite, biotite, and ilmenite, but not garnet, have undergone isotopic exchange with surrounding trondjemite.

With few exceptions, quartz-magnetite and quartz-ilmenite fractionations decrease with increasing metamorphic grade determined by mineral paragenesis and spatial distribution. This consistency does not apply to quartz-magnetite and quartz-ilmenite fractionations obtained from rocks in which petrographic evidence of retrogradation is present.

Whereas measured isotopic fractionations among quartz, garnet, ilmenite, and magnetite are approximately related to metamorphic grade, fractionations between these minerals and biotite or muscovite show poor correlation with grade. Variations in muscovite-biotite fractionations are relatively small. These observations are interpreted to mean that muscovite and biotite are affected by retrograde re-equilibration to a greater extent than the anhydrous minerals analyzed.

Measured quartz-ilmenite fractionations range from 12 permil in the biotite zone of central Vermont to 6.5 permil in the sillimanite-orthoclase zone of southeastern Connecticut. Analyses of natural assemblages from the kyanite and sillimanite zones suggest that equilibrium quartz-ilmenite fractionations are approximately 8 percent smaller than corresponding quartz-magnetite fractionations. Employing the quartz-magnetite geothermometer calibrated by O'Neil and Clayton (1964), a temperature of 560°C was obtained for kyanite-bearing schists from Addison County, Vermont. Extending the calibration to quartz-ilmenite fractionations, a temperature of 600°C was obtained for kyanite-schists from Shoshone County, Idaho. At these temperatures kyanite is stable only at pressures exceeding 11 kbars (Bell, 1963), corresponding to lithostatic loads of over 40 km.

Physical, Metabolic, and Energetic Investigations of Methane-Metabolizing Microbial Communities

Understanding the roles of microorganisms in environmental settings by linking phylogenetic identity to metabolic function is a key challenge in delineating their broad-scale impact and functional diversity throughout the biosphere. This work addresses and extends such questions in the context of marine methane seeps, which represent globally relevant conduits for an important greenhouse gas. Through the application and development of a range of culture-independent tools, novel habitats for methanotrophic microbial communities were identified, established settings were characterized in new ways, and potential past conditions amenable to methane-based metabolism were proposed. Biomass abundance and metabolic activity measures – both catabolic and anabolic – demonstrated that authigenic carbonates associated with seep environments retain methanotrophic activity, not only within high-flow seep settings but also in adjacent locations exhibiting no visual evidence of chemosynthetic communities. Across this newly extended habitat, microbial diversity surveys revealed archaeal assemblages that were shaped primarily by seepage activity level and bacterial assemblages influenced more substantially by physical substrate type. In order to reliably measure methane consumption rates in these and other methanotrophic settings, a novel method was developed that traces deuterium atoms from the methane substrate into aqueous medium and uses empirically established scaling factors linked to radiotracer rate techniques to arrive at absolute methane consumption values. Stable isotope probing metaproteomic investigations exposed an array of functional diversity both within and beyond methane oxidation- and sulfate reduction-linked metabolisms, identifying components of each proposed enzyme in both pathways. A core set of commonly occurring unannotated protein products was identified as promising targets for future biochemical investigation. Physicochemical and energetic principles governing anaerobic methane oxidation were incorporated into a reaction transport model that was applied to putative settings on ancient Mars. Many conditions enabled exergonic model reactions, marking the metabolism and its attendant biomarkers as potentially promising targets for future astrobiological investigations. This set of inter-related investigations targeting methane metabolism extends the known and potential habitat of methanotrophic microbial communities and provides a more detailed understanding of their activity and functional diversity.

Comportamento territorial de Hylodes nasus em um riacho na Floresta da Tijuca, Rio de Janeiro.

O comportamento territorial é exibido por animais que competem por recursos dentro de uma área específica defendida. Ao excluir competidores potenciais desta área através de comportamentos agressivos, os indivíduos territoriais garantem acesso prioritário aos recursos essenciais para sua sobrevivência e reprodução. Em anfíbios anuros, a territorialidade tem sido relacionada principalmente com espécies de reprodução prolongada, cujos sítios reprodutivos estão disponíveis durante todo o ano. O presente trabalho foi realizado em um trecho de riacho na Floresta da Tijuca, Rio de Janeiro. Foram realizadas quatro visitas consecutivas a área de amostragem em cada mês (abril/2009 à agosto/2010). O objetivo foi responder questões sobre a territorialidade dos indivíduos da espécie Hylodes nasus: (área de vida, fidelidade ao sítio, comportamentos agonísticos e combates físicos). As áreas de vida dos machos apresentaram relação significativa com o número de recapturas. O tamanho da área de vida das fêmeas foi ligeiramente maior do que dos machos. Os machos apresentaram maior fidelidade do que as fêmeas. A fidelidade apresentou resultado significativo com o CRC e a massa corporal dos indivíduos. Foram observadas 50 interações agonísticas entre machos, sendo exibidos 11 comportamentos agressivos. Foram observados 19 combates físicos, sendo os machos envolvidos classificados como residente, intruso, vencedor ou perdedor. Todas as disputas foram vencidas por indivíduos residentes. Informações a respeito da influência de fatores ambientais sobre a população de H. nasus, aspectos da estrutura populacional e descrição do microhábitat também são fornecidos.

Herpetofaunal Inventories of the National Parks of South Florida and the Caribbean: Volume II. Virgin Islands National Park

Amphibian declines and extinctions have been documented around the world, often in protected natural areas. Concern for this alarming trend has focused attention on the need to document all species of amphibians that occur within U.S. National Parks and to search for any signs that amphibians may be declining. This study, an inventory of amphibian species in Virgin Islands National Park, was conducted from 2001 to 2003. The goals of the project were to create a georeferenced inventory of amphibian species, use new analytical techniques to estimate proportion of sites occupied by each species, look for any signs of amphibian decline (missing species, disease, die-offs, etc.), and to establish a protocol that could be used for future monitoring efforts. Several sampling methods were used to accomplish these goals. Visual encounter surveys and anuran vocalization surveys were conducted in all habitats throughout the park to estimate the proportion of sites or proportion of area occupied (PAO) by amphibian species in each habitat. Line transect methods were used to estimate density of some amphibian species and double observer analysis was used to refine counts based on detection probabilities. Opportunistic collections were used to augment the visual encounter methods for rare species. Data were collected during four sampling periods and every major trail system throughout the park was surveyed. All of the amphibian species believed to occur on St. John were detected during these surveys. One species not previously reported, the Cuban treefrog (Osteopilus septentrionalis), was also added to the species list. That species and two others (Eleutherodactylus coqui and Eleutherodactylus lentus) bring the total number of introduced amphibians on St. John to three. We detected most of the reptile species thought to occur on St. John, but our methods were less suitable for reptiles compared to amphibians. No amphibian species appear to be in decline at this time. We found no evidence of disease or of malformations. Our surveys provide a snapshot picture of the status of the amphibian species, so continued monitoring would be necessary to determine long-term trends, but several potential threats to amphibians were identified. Invasive species, especially the Cuban treefrog, have the potential to decrease populations of native amphibians. Introduced mammalian predators are also a potential threat, especially to the reptiles of St. John, and mammalian grazers might have indirect effects on amphibians and reptiles through habitat modification. Finally, loss of habitat to development outside the park boundary could harm some important populations of amphibians and reptiles on the island.

Herpetofaunal Inventories of the National Parks of South Florida and the Caribbean: Volume III. Big Cypress National Preserve

Amphibian declines and extinctions have been documented around the world, often in protected natural areas. Concern for this trend has prompted the U.S. Geological Survey and the National Park Service to document all species of amphibians that occur within U.S. National Parks and to search for any signs that amphibians may be declining. This study, an inventory of amphibian species in Big Cypress National Preserve, was conducted from 2002 to 2003. The goals of the project were to create a georeferenced inventory of amphibian species, use new analytical techniques to estimate proportion of sites occupied by each species, look for any signs of amphibian decline (missing species, disease, die-offs, and so forth.), and to establish a protocol that could be used for future monitoring efforts. Several sampling methods were used to accomplish these goals. Visual encounter surveys and anuran vocalization surveys were conducted in all habitats throughout the park to estimate the proportion of sites or proportion of area occupied (PAO) by each amphibian species in each habitat. Opportunistic collections, as well as limited drift fence data, were used to augment the visual encounter methods for highly aquatic or cryptic species. A total of 545 visits to 104 sites were conducted for standard sampling alone, and 2,358 individual amphibians and 374 reptiles were encountered. Data analysis was conducted in program PRESENCE to provide PAO estimates for each of the anuran species. All of the amphibian species historically found in Big Cypress National Preserve were detected during this project. At least one individual of each of the four salamander species was captured during sampling. Each of the anuran species in the preserve was adequately sampled using standard herpetological sampling methods, and PAO estimates were produced for each species of anuran by habitat. This information serves as an indicator of habitat associations of the species and relative abundance of sites occupied, but it will also be useful as a comparative baseline for future monitoring efforts. In addition to sampling for amphibians, all encounters with reptiles were documented. The sampling methods used for detecting amphibians are also appropriate for many reptile species. These reptile locations are included in this report, but the number of reptile observations was not sufficient to estimate PAO for reptile species. We encountered 35 of the 46 species of reptiles believed to be present in Big Cypress National Preserve during this study, and evidence exists of the presence of four other reptile species in the Preserve. This study found no evidence of amphibian decline in Big Cypress National Preserve. Although no evidence of decline was observed, several threats to amphibians were identified. Introduced species, especially the Cuban treefrog (Osteopilus septentrionalis), are predators and competitors with several native frog species. The recreational use of off-road vehicles has the potential to affect some amphibian populations, and a study on those potential impacts is currently underway. Also, interference by humans with the natural hydrologic cycle of south Florida has the potential to alter the amphibian community. Continued monitoring of the amphibian species in Big Cypress National Preserve is recommended. The methods used in this study were adequate to produce reliable estimates of the proportion of sites occupied by most anuran species, and are a cost-effective means of determining the status of their populations.

Using RIVPACS for studies on conservation and biodiversity

Invertebrate conservation relies not only on public support and political will, but also on possessing an adequate understanding of the distribution and ecology of invertebrate species and communities. In the UK, RIVPACS is making an important contribution to assessing the conservation importance of river invertebrate assemblages. So far, work has largely centred on using RIVPACS as an integral part of SERCON (System for Evaluating Rivers for Conservation), in which data collected using the standard RIVPACS method are interpreted with reference to conservation criteria such as species richness and representativeness. Applications of RIVPACS to other areas of conservation - whether providing information on the ecological requirements of rare species, monitoring the success of river restoration projects, or making broader assessments of sustainability - are probably more limited, but merit further examination. It is important to develop closer links between RIVPACS and techniques such as SERCON and RHS (River Habitat Survey) in order to maximise the benefit each can bring tostudies on conservation and biodiversity. It should also be recognised that there are limitations in transferring such systems to other countries where approaches to nature conservation may be very different.

A comunidade de anuros em uma área de floresta ombrófila densa montana do Parque Estadual dos Três Picos, sudeste do Brasil

O conhecimento sobre a fauna de anfíbios em áreas de altitude (> 1000 m) da Mata Atlântica do sudeste do Brasil é ainda insuficiente. O Parque Estadual dos Três Picos (PETP), no estado do Rio de Janeiro, constitui um dos maiores remanescentes de Mata Atlântica do estado inserido em uma Unidade de Conservação. Os limites do PETP abrangem um amplo gradiente altitudinal, alcançando mais de 2000 m de elevação e protegem áreas de cinco municípios do estado do Rio de Janeiro. Neste estudo, eu apresento dados de três anos de amostragem e pesquisa (2008-2010) sobre a composição e a abundância de espécies de anfíbios em área de altitude localizada entre 1100 e 1400 m no PETP, distrito de Theodoro de Oliveira, município de Nova Friburgo, estado do Rio de Janeiro, Brasil. O esforço amostral total foi de 360 horas de buscas ativas, padronizado em 180 horas de procura em cada uma das estações do ano (úmida e seca). Foram registradas 32 espécies de anuros durante o estudo, das quais 18 representaram novos registros para o Parque e três novas espécies ainda não conhecidas da Ciência foram descobertas (Holoaden pholeter, recentemente descrita, foi uma delas). As espécies mais abundantes foram os anuros de desenvolvimento direto Ischnocnema parva e I. erythromera. Nove das 32 espécies encontradas são consideradas endêmicas do estado do Rio de Janeiro: Brachycephalus sp., B. didactylus, B. garbeanus, Ischnocnema cf. holti, I. erythromera, Bokermannohyla carvalhoi, Scinax albicans, H. pholeter e Hylodes charadranaetes. Houve diferença em alguns parâmetros da comunidade entre as estações e entre as faixas de altitude amostradas, com uma maior riqueza e abundância de anuros na estação úmida do que na seca, e mudança na composição de espécies entre as estações. Apesar de nenhuma das espécies registradas durante o estudo constar na lista de espécies ameaçadas, H. pholeter pode ser um candidato à inclusão na categoria vulnerável da Lista das Espécies Ameaçadas de Extinção da IUCN, devido à sua distribuição geográfica conhecida ser restrita apenas à localidade tipo (Theodoro de Oliveira) e à sua densidade populacional ser aparentemente baixa. . A alta diversidade de anfíbios, com espécies endêmicas ao estado, e a ocorrência de espécies raras atestam a relevância biológica das áreas estudadas no PETP.

Os anfíbios da restinga de Grumari, RJ, Brasil, e a comunidade de folhiço em duas zonas arbóreas

Apesar dos habitats de restinga representarem aproximadamente 5.000 km da costa brasileira, o conhecimento disponível sobre a composição da fauna de anuros nestes habitats ainda é restrito a poucas áreas. Estes habitats encontram-se sob intensa pressão imobiliária, com uma densidade demográfica cinco vezes maior do que a média do país, levando a uma ocupação descomedida, degradação e eventual perda destes importantes habitats. Isso é especialmente preocupante para as populações de vertebrados terrestres endêmicos e ameaçados de extinção, devido à dificuldade de recuperação da vegetação das restingas. Atualmente, os remanescentes de restinga ao longo da costa brasileira são representados por fragmentos completamente isolados. Esta é a primeira aproximação da lista de anfíbios da restinga de Grumari, inserida no Parque Natural Municipal de Grumari (PNMG), município do Rio de Janeiro, sudeste do Brasil. É também a primeira pesquisa a analisar a comunidade de anuros de folhiço, e a obter dados de densidade, para as restingas brasileiras. A área está situada no Domínio da Mata Atlântica. O estudo foi conduzido de maio de 2009 a dezembro de 2010 utilizando dois métodos de amostragem: parcelas grandes ou plots de 16 m (4x4 m) e transecções visuais/acústicas. Registramos 22 espécies de anfíbios anuros distribuídas em seis famílias: Leptodactylidae (3), Craugastoridae (1), Bufonidae (1), Strabomantidae (1), Microhylidae (2) e Hylidae (14). Ocorreram dez espécies de anuros de folhiço em 2560 m de chão de floresta amostrados com a metodologia de parcelas grandes durante o estudo. Na zona fechada de pós-praia ocorreram apenas sete espécies de anuros de folhiço, enquanto na mata de restinga ocorreram as dez espécies registradas para a restinga. A densidade geral estimada de anuros de folhiço para a restinga de Grumari foi de 13,1 indivíduos por 100m, superior a cinco de seis estudos publicados com dados de densidade de anuros de folhiço na Mata Atlântica. A biomassa total estimada foi de 13131 g/ha, podendo ser considerada a maior biomassa de anurofauna de folhiço globalmente. Não houve diferença estatisticamente significativa entre as zonas quanto aos parâmetros da comunidade (riqueza, densidade e biomassa), mesmo havendo diferença entre os parâmetros ambientais mensurados nas duas zonas. Os fatores que melhor explicaram a riqueza, a densidade e a biomassa na restinga de Grumari durante a estação chuvosa foram, respectivamente, a temperatura, o diâmetro das árvores/arbustos e o número de árvores/arbustos. A presença de espécies ameaçadas de extinção e endêmicas para o estado do Rio de Janeiro e Mata Atlântica evidencia que a restinga de Grumari é uma área essencial para a conservação da anurofauna dos remanescentes deste habitat no Brasil. Além disso, Grumari é a restinga com maior riqueza de espécies do estado e a segunda maior entre todas as áreas de restinga estudadas até o momento, quanto à anurofauna.