曲面效应法优化辅酶Q_(10)发酵条件

以根癌土壤杆菌(Agrobacterium tum efaciens)1.1416的突变株AGR 0610为出发菌株,采用P lackett-Burm an设计法(P lackett-Burm an design,P-B)对影响AGR 0610发酵产辅酶Q10的相关因素进行了研究,选取到4种有显著效应的因素:糖蜜、大豆蛋白胨、玉米浆和蛋氨酸.再采用响应曲面法(response surface m ethodology,RSM)对这4种因素的最佳水平范围进一步探讨,得到二次回归方程,优化了培养基组成.当这4种因素分别取值为1.02%、0.49%、0.29%和0.34%时,辅酶Q10产量有最大预测值38.88 mg/L,并被实验所证实.图6表4参8

生态空间理论与景观异质性

概述生态空间理论的若干主要方面：尺度、空间格局、景观异质性、镶嵌与生态交错带以及干扰与景观动态等。生态空间理论是景观评价、管理和生态规划的重要基础，对于区域可持续发展和生物多样性保护等方面的研究都具有重要的指导意义。

生物有机肥对小油菜生长及品质的影响

在小油菜上进行施用生物有机肥、有机无机配合施肥、三元肥的田间对比试验。结果表明,单施生物有机肥比单施无机三元肥的小油菜中硝酸盐和亚硝酸盐含量分别降低36.17%和21.77%;种植后土壤中有机质比单施无机三元肥提高11.12%。油菜生产中施用生物有机肥可以提高作物品质,提高土壤有机质含量,培肥地力,与三元肥配合施用不会造成明显减产。

树木、林内、林冠上与森林区风速廓线研究(英文)

风速是森林生态系统研究中最为重要的变量之一,同时也是控制气流运输过程最基本的要素。由于树木生理指标和生长过程对风速的改变十分敏感,因此风速随高度的变化规律,即风速廓线的研究十分重要。一般认为,在裸地或林冠上的风廓线均呈对数规律变化,但是在单株树木和林分内风速随高度的变化则不呈对数规律。本文根据最近在海岸松林内风的研究结果,总结了针叶树种单株树木内、林分内和林冠上层以及海岸林区的风速廓线变化规律,其主要结论如下:1)单株针叶树树冠内的风速廓线呈指数形式分布,2)在林分内的风速廓线可用风的减弱系数来表示,3)[(\267\347)(\300\252\317\337\324\332\301\326\271\332\262\343)]上的分布可由风廓线参数(摩擦速度、粗糙长度、零平面位移)确定,4)在海岸林区,极端风速的分布可以使用了建筑上的风荷载模型进行预测。另外,本文提出了该研究领域尚需进一步探讨的问题,主要包括:1)风速廓线与树木特征及林分特征间的关系,2)利用简单的方法预测了风速廓线参数,3)风速廓线在树木生理、生态研究的作用、树木生长过程中风的微生态学效应以及森林生态和管理应用等方面。图4表1参60。

水稻生育后期生化特性分析

以2个不同穗型品种辽粳326(直立型)和奥羽316(弯穗型)为试材,测定了水稻生育后期生理生化指标,每次取倒2叶进行测定,包括超氧化物歧化酶(SOD)、过氧化物酶(POX)、过氧化氢酶(CAT)和可溶性蛋白质含量。从测定结果来看,抽穗前辽粳326各项指标明显高于奥羽316,抽穗以后各项指标变化有所不同,体现了两品种不同的衰老特性。

异胡豆苷合成酶在烟草亚细胞区室的表达(英)

异胡豆苷合成酶 (strictosidinesynthase,STR)是吲哚生物碱生物合成的一种关键酶 ,将色胺 (tryptamine)和裂环马钱子 (secologanin)耦合成为吲哚生物碱的前体化合物异胡豆苷。将异胡豆苷合成酶标定在烟草植物不同的亚细胞区室———叶绿体、液泡和内质网中表达 ,通过蛋白免疫印迹分析和STR酶活性的测定 ,表明STR在叶绿体、液泡和内质网中有效表达。STR体外酶活性分析采用间接荧光法检测色胺在反应体系的消耗。STR的酶活性分析表明了STR在烟草中不同的亚细胞区室得以活性表达。分离纯化转基因烟草的叶绿体 ,通过对其分离的不同部分的蛋白免疫印迹分析 ,确定了将STR正确标定在烟草的叶绿体中表达。

中国植被净生产力的比较研究

利用3个光能利用率模式(CASA,GLOPEM和GEOLUE)和2个生态过程机理模式(CEVSA和GEOPRO)以不同空间分辨率和不同输入参数对中国植被净生产力(NetPrimaryProduction,NPP)进行时空模拟,对5个模式模拟的中国的NPP进行了时间序列和空间格局的对比分析所得结论如下:CASA,GLOPEM及CEVSA3个模式对中国NPP的月、季和年的时空模拟符合中国植被季节变化规律和季风气候下的中国植被的空间变化规律,近20a来中国NPP变化趋势以增长为主;5个模式模拟的中国平均NPP季节的值为春季约为(0.571±0.2)GtC左右,夏季约为(1.573±0.4)GtC左右,秋季约为(0.6±0.2)GtC左右,冬季约为(0.12±0.1)GtC左右;中国植被净生产力年值约为(2.864±1)GtC/a;5个模式较好地模拟了中国不同类型生态群落的生物量的季节特征和空间格局状况.研究为中国利用造林、再造林、森林和农田管理等人为活动引起的碳增汇用于抵消中国承诺的温室气体减排指标的计算及碳收支平衡的研究提供参考,为植被净生产力总量的国家本底的确定提供了依据.

水文非线性系统理论与方法

水是支撑地球生命生态系统和社会经济发展不可替代的资源。为了研究水文现象的变化和陆地水文循环的规律，水文系统方法论等到了发展。本书是关于水文非线性系统理论与方法的一本著作。它既有作者对过去水文系统非线性系统识别理论研究的成果总结，也有作者最近几年开展的环境变化下流域水文循环复杂性探索以及分布式流域水循环模拟与应用的成果。全书分为七章，分别从水文循环及水文系统的基本概念、水文非线性系统识别原理、水文非线性系统描述的泛函模型方法、集总式的水文非线性概念性模型途径，以及流域分布式水循环系统多个层面，阐述水文非线性系统模拟、水文预报、水量转化分析、水资源评价和水文生态系统的特征及应用实例。

本书可供地球科学、水利工程、农学、环境类的水文水资源及其相关专业研究者、教师、研究生和大学高年级学生阅读和参考。   

Effects of cerium on the microstructure and mechanical properties of Mg–20Zn–8Al alloy

Mg-20Zn-8Al-xCe(x=0-2 wt.%) alloys were prepared by metal mould casting method, the effects of Ce on the microstructure and mechanical properties of the alloys were investigated. The results showed that the dendrite as well as gram size were refined by the addition of Ce, and the best refinement was obtained in 1.39% Ce containing alloy.

Layer-by-layer assembly of carbon nanotubes and Prussian blue nanoparticles: A potential tool for biosensing devices

A promising method for assembling carbon nanotubes (CNTs) and poly(diallyldimethylammonium chloride) protected Prussian blue nanoparticles (P-PB) to form three-dimensional (3D) nanostructured films is proposed. The electrostatic interaction, combined with layer-by-layer self-assembly (LBL), between negatively charged CNTs and positively charged P-PB is strong enough to drive the formation of the 3D nanostructured films. Thus, prepared multilayer films were characterized by ultraviolet-visible-near-infrared spectroscopy (UV-vis-NIR), scanning electron microscopy (SEM) and cyclic voltammetry (CV).

One Pot, Facile Synthesis of Hierarchical Silver Nanostrip Assembling Architecture

Here we present a simple wet-chemical approach to synthesize flower-like silver nanostrip assembling architecture at room temperature. Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) images indicate that these microstructures with the diameter of similar to 500nm exhibit hietarchical characteristic. X-ray diffraction (XRD), energy-dispersed X-ray spectroscopy (EDX) and Raman spectroscopy indicate that poly (o-diaminobenzene) (PDB) also exists in the silver hierarchical microstructure.

Dendrimers as "controllers" for modulation of electrodeposited silver nanostructures

Electrodeposition of silver nanostructures on a polyamidoamine (PAMAM) dendrimers-modified surface has been reported. The assembled PAMAM monolayer film was used as a substrate for electrodeposition. We found that the PAMAM dendrimers obviously affected nucleation growth and silver nanostructures (spherical, dendritic and "fish bone" shapes) were obtained, which were different from those deposited on unmodified surfaces. It was attributed to the unique structures and properties of PAMAM dendrimers compared with linear polymers.

A zinc/p-sulfonatocalix[4]arene/phenanthroline supramolecular compound with novel (H2O)(10) water clusters

A supramolecular complex Zn-2[Cl2H8N2](2)[C28H2OS4O16][H2O](17.7) (1), has been synthesized under hydrothermal conditions, and characterized by IR spectroscopy, TG and DTA analysis, and single crystal X-ray diffraction. Unprecedented (H2O)(10) water clusters consisting of cyclic pentamer (H2O)(5) and five dangling water molecules were observed in the lattice.

Self-assembly of lambda-DNA networks/Ag nanoparticles: Hybrid architecture and active-SERS substrate

In this article, highly rough and stable surface enhanced Raman scattering (SERS)-active substrates had been fabricated by a facile layer by-layer technique. Unique lambda-DNA networks and CTAB capped silver nanoparticles (AgNP) were alternatively self-assembled on the charged mica surface until a desirable number of bilayers were reached. The as-prepared hybrid architectures were characterized by UV-vis spectroscopy, tapping mode atomic force microscopy (AFM) and confocal Raman microscopy, respectively.