Prolonged cardiac allograft survival in presensitized rats after a high activity Yunnan-cobra venom factor therapy

Complement-dependent antibody-mediated acute humoral rejection is the major obstacle of clinical transplantation across ABO incompatibility and human leukocyte antigen presensitization. We previously demonstrated that Yunnan-cobra venom factor (Y-CVF) cou

Islet-1 is required for ventral neuron survival in Xenopus

Islet-1 is a LIM domain transcription factor involved in several processes of embryonic development. Xenopus Islet-1 (Xisl-1) has been shown to be crucial for proper heart development. Here we show that Xisl-1 and Xisl-2 are differentially expressed in th

Salinity and survival of Martesia striata (Linn) in Cochin harbour

The effects of salinity variations on the survival of Martesia striata from Cochin harbour are presented. It is observed that at least a few of the animals survive the low saline conditions during monsoon. Laboratory experiments showed the lethal salinity as 6‰ when animals acclimatised in 34‰ were subjected to abrupt changes in salinity. But acclimatisation to 17‰ salinity showed a downward shift in the lethal salinity to 4‰. The present observations indicate that M. striata is euryhaline arid the extent of tolerance to lower salinities depends on the degree of acclimatisation.

Temporal behaviour of marine landings along coastal Karnataka 1. Relative distribution and secular trends

Landing data of 21 fisheries from Karnataka (quarterly landings for the period 1956 to 1978 and annual landings for the period 1979 to 1981) were analysed to study the te111poral behaviour of the landings. The relative distribution and secular trends in each of the fisheries are reported. On an average, mackerel and oil sardine together account for about 70% of the annual landings. The fluctuation in the landings in all the fisheries was very high with the coefficient of variation attaining a minimum value of 61.53%. The inverse relationship between the landings of oil sardine and mackerel was found to be statistically significant. It is suggested that fishing effort be further increased in fisheries that have demonstrated a rising trend (about 73% of the total annual landings) as also in the case of the fisheries whose trends appear stagnant (about 21% of the annual landings). However, there seems to be some evidence of a need for regulating fishing in Hemirhamphus and Belone, Caranx and penaeid prawns. Regulations need to be made authentic with the help of sufficient information on not merely the fishery but on the whole population.

The effect of various protein levels on the growth and survival rates of Penaeus monodon Fabricius

Postlarvae of tiger prawns, P. monodon , were fed with various protein levels of 0; 30; 40 and 50%, fish meal and rice bran were combined and pelletized for 16 wks. Prawns used had an average mean length of 1.2 mm and an average mean weight of 5.5 mg. Nitrite, ammonia, dissolved oxygen content, temperature and pH were monitored. After one month of feeding, growth increments of postlarvae fed with 30% protein diet were significantly different from those given 0; 40 and 50%. Survival rate of the group fed 40% protein was higher (81.7%) but was not significantly different from those fed 30% (68.0%). The highest feed efficiency was obtained from the 30% protein pellets (4.6). At the end of 16 weeks, growth increment of 40% protein level was significantly different from the 30% and 50% protein levels, but the survival rate was not signficantly different from the 30% protein level. Feed conversion for the 40% protein diet was 2.8.

Survival rates of different postlarval stages of Penaeus monodon Fabricius

The objective of this study is to determine survival rates of different postlarval stages upon stocking in the Leganes ponds. Twelve 3m x 2m x 2m suspension nets made of nylon cloth (mesh size = 0 . 1 mm) were set up in a Leganes Station pond (ave. water depth = 1 m) by means of 3-m long poles stacked at distances approximating the area of each net. The net bottom was filled with topsoil at least 15 cm thick to stimulate the pond bottom. At least 60 cm of the upper edge of each net was above the water level to prevent mixing of water inside and outside the net. P.monodon of stages P SUB-11 , P SUB-15 , P SUB-21 (from the hatchery) and P SUB-25 (from the wet lab) were stocked in the nets at 200/sq m or 1,200 fry/net. Due to lack of fry, only one P SUB-25 net was stocked. Each net had two large dried miapi branches as shelter from predation and cannibalism for the young sugpo fry. Fresh lablab was fed at the rate of one pail (approximately 5 kg) every four days per net. Harvest data show relatively higher survival rates for P SUB-15 and P SUB-18 compared to P SUB-11 and P SUB-25 with no significant difference between these two stages. The results for P SUB-25 may not be valid because the stock came from the wet lab in comparison to the other postlarval stages which were reared in the hatchery. Moreover, the P SUB-25 stock had no replicates and the net itself (no. 10) was discovered to have many holes. These preliminary results point to P SUB-15 as the best stage for harvest from the hatchery in terms of high pond recovery and lesser expense in rearing compared to older postlarvae.

Effect of some physico-chemical factors on the survival and growth of Penaeus monodon postlarvae

Growth and survival rates of P. monodon postlarvae were examined at different temperatures, salinities, and nitrite and ammonia concentrations, using one feed level. Condition of postlarvae greatly affected the experimental results shown in some instances where very low survival rates were obtained, even for the controls. Results indicated that postlarvae from PSUB-10 and up can tolerate salinity changes of 10 to 20 ppt without prior acclimation. Survival generally appears the same for temperatures between 24 and 36 C. It appears that P. monodon postlarvae have higher temperature tolerance. Tolerance of postlarvae at the early postlarval stage is between 30 and 50 ppm of nitrate. They were more tolerant from PSUB-10 upwards. Although survival was high in runs containing nitrite, growing appears to have been affected. Postlarvae could tolerate ammonia concentrations up to about 50 ppm. At 100 ppm higher mortality rates were observed. Whether or not there was any permanent effect by nitrate and ammonia at high but apparently tolerable levels is not known.

Length-weight relationship and relative condition factor in Daysciaena albida (Cuv.) and Gerres filamentosus (Cuv.)

The length-weight relationship of Daysciaena albida and Gerres filamentosus were calculated separately for indeterminants, mature males and mature females. The logarithmic regression equation obtained for D. albida - males: log w = -1.5055 + 2.8618 log l; females: log w = -0.9260 + 2.4089 log l; indeterminants: log w = -l.7188 + 3.0616 log l. The regression co-efficients between males and females, males and in determinants and female and in determinants showed significant differences. In G. filamentosus the relationship can be expressed as males: log w = -1.3224 + 2.8740 log 1; females: log w = -1.2874 + 2.8381 log l; indeterminants: log w = -0.8167 + 2.2558 log l. The difference in regression co-efficients between male and female are insignificant at 5% level whereas significant differences were observed between males and indeterminants and females and indeterminants. The relative condition factor (Kn) was calculated for the above two species. In D. albida the reasons for the fluctuations of Kn values can be attributed to both spawning cycle as well as feeding intensity whereas in G. filamentosus it synchronies mainly with spawning cycle.

Effect of liquid nitrogen freezing and subsequent storage on survival of Staphylococcus aureus and Streptococcus pyogenes in treated prawn meat

Prawn meat treated with Streptococcus pyogenes B-49-2 culture and Staphylococcus aureus ATCC-12598 culture were frozen in conventional plate freezer at -40°C and by spray type liquid nitrogen freezer. The frozen products were stored at -18°C. Streptococcus pyogenes B-49-2 showed low sensitivity to cold injury during freezing and frozen storage. Staphylococcus aureus ATCC-12598 survived during the entire storage period of 240 days. Total bacterial count of untreated prawn meat was found to be always lesser in liquid nitrogen frozen products than that in plate frozen products.

Study of the relative effectiveness of extension methods in educating fisherwomen

Three experimental groups from three different fishing villages were selected and administered with three extension treatments on two messages, namely, production of fish wafers and fish pickles. There was a significant knowledge gain in the subjects taught through different extension methods. It was observed that lecture aided with slides induced maximum knowledge followed by lecture aided with charts and lecture alone. Among all, the young and highly educated women gained maximum knowledge.

Preliminary studies on growth and survival of Penaeus japonicus postlarvae fed with Tapes and commercial formula feeds

Growth and survival of P. japonicus postlarvae was studied using Tapes philippinarum and commercial formula feeds. After a 28-day feeding period, the highest survival rate of 100% was obtained in prawns receiving Diet-B, followed by those on Tapes Kyowa Hakko shrimp meal with survival rates of 80 and 76.7%, respectively. The lowest survival rate of 48.3% was obtained among postlarvae fed with squid meal. In this batch cannibalism was observed which resulted in low survival. Growth was highest in the postlarvae fed with Kyowa Hakko and mysid feeds. Diet-B gave a slightly lower weight increase but gave similar gain in length. The composition of the artificial diet, Diet-B, and growth and survival rates are tabulated.

Growth and survival of Penaeus monodon postlarvae fed shrimp head meal and fish meal as primary animal source of protein

Although shrimp head meal alone does not provide for good growth and survival, fish meal can provide high survival rate. The addition of shrimp head improves this diet. It is suggested that cholesterol present in shrimp could have caused this difference. Composition of the test diets is tabulated, as are proximate chemical analysis of the diets, and the mean initial weights, final weights, weight gains, survival rate, feed consumed, protein consumed, of Penaeus monodon postlarvae, feed conversion and protein efficiency ratio.

Incidence and low temperature survival of coagulase positive staphylococci in fishery products

Coagulase-positive staphylococci was found to be absent in all the frozen samples of lobsters, cuttle fish, cat fish, seer fish and red snapper examined. Coagulase-positive staphylococci were present in 38% of the cooked frozen shrimps and only 16% of the samples had staphylococci count more than 100/g. In the case of headless, peeled and deveined, peeled undeveined shrimps, the incidence of the organism was 6, 12 and 16% respectively. The study indicated that the incidence of coagulase-positive staphylococci is not a serious problem in frozen fishery products processed in this country. There was remarkable difference in the rate of destruction of coagulase-positive staphylococci in raw and cooked shrimps during freezing and frozen storage.

Preserved algae as food for Penaeus monodon larvae

Uni-algal cultures of C. calcitrans, S. costatum, T. chui and Isochrysis sp. obtained from the laboratory were harvested. The harvest, preserved by either freezing or sun-drying, was fed to the larvae of P. monodon . Among the test algal species, Chaetoceros and Tetraselmis were used in larval feeding trials with frozen food while Chaetoceros, Tetraselmis and Isochrysis species were utilized in feeding experiments with sun-dried algae. Their relative effects on larval survival and development were assessed. Results showed that, except the alum-flocculated cells, both frozen Chaetoceros and Tetraselmis can support survival at the zoea stage. Best survival of 68% was atained with dried Chaetoceros followed by Tetraselmis at 44%. Dried Isochrysis did not perform as well, a significantly low survival of only 25% was obtained.