Estrategias metodológicas para operacionalizar la práctica educativa transdisciplinaria, en conjunto con los actores universitarios, en las licenciaturas del Centro de Estudios Universitarios Arkos (CEUArkos) de Puerto Vallarta, Jalisco, México

Recibido 29 de noviembre de 2010 • Aceptado 09 de marzo de 2011 • Corregido 12 de abril de 2011 El artículo resume los procesos vividos y los resultados obtenidos a través de una investigación-acción con actores (profesores, estudiantes y directivos) de las distintas áreas del CEUArkos de Puerto Vallarta, Jalisco, México. Su propósito es construir y llevar a la práctica estrategias metodológicas para operacionalizar la transdisciplinariedad y la complejidad en el aprendizaje, la enseñanza y la investigación –como parte de la docencia– en las licenciaturas de la institución, a fin de sentar las bases para el paso de una formación universitaria que transite de la disciplinariedad hacia la transdisciplinariedad en todas las carreras que la Universidad oferta. Aborda, asimismo, el tema del currículo universitario.

Necesidades académicas, personales, sociales y vocacionales de estudiantes internacionales e intercambio en la Universidad de Puerto Rico, Recinto de Río Piedras

Recibido 20 de setiembre de 2011 • Aceptado 24 de octubre de 2011 • Corregido 14 de noviembre de 2011   El propósito de este estudio fue identificar las necesidades de una muestra de estudiantes internacionales y de intercambio que cursan estudios en la Universidad de Puerto Rico, Recinto de Río Piedras. Se exploraron datos socio-demográficos así como las necesidades académicas, vocacionales, y personales/sociales, con los fines de recopilar información de relevancia para universidades que sirven esta población y someter recomendaciones que atiendan las necesidades. Los hallazgos revelaron necesidades mayormente en las áreas académicas y vocacionales. Entre estas, se destacan la necesidad de aprender inglés o español, y el conocer sobre internados y ofrecimientos académicos en Puerto Rico y fuera del país, sueldo y oportunidades de empleo, ayudas económicas y servicios estudiantiles en la Universidad de Puerto Rico. Se proveen recomendaciones para trabajar con las necesidades identificadas ya que, según establece la bibliografía, las dificultades que confrontan estos/as estudiantes pueden afectar su desempeño académico e incluso su salud emocional.

ANÁLISIS DE TENDENCIAS HIDROCLIMÁTICAS DE DOS CUENCAS REPRESENTATIVAS DE ISLA DE PUERTO RICO

El agua junto a las variaciones climáticas, encabezan discursos políticos y científicos globales en los que resurgen los debates sobre los conflictos acerca de este recurso, la geopolítica del agua, la gestión, la contaminación y la polución. Ante este panorama el IPCC (Panel Intergubernamental del Cambio Climático), la Organización Mundial de Meteorología (OMM),el Programa de la Naciones Unidas para el Medio Ambiente (PNUMA) y distintas organizaciones medioambientales hanenfocado sus intereses científicos en estudios en los cuales se utilicen indicadores hidroclimáticos. En este contexto, este artículo analiza la evolución hidroclimática reciente en dos cuencas principales de la isla de Puerto Rico, utilizando como indicadores las temperaturas, las precipitaciones y los caudales de distintas estaciones de NOAA y el USGS. Inicialmente analizamos cada estación individualmente y luego se hizo un análisis conjunto de la cuenca. Con los resultados obtenidos,en ninguna de las cuencas se pudo establecer tendencias claras en cuanto a la precipitación total anual. Aunque algunas estaciones mostraron tendencias positivas y otras regresivas, ninguna alcanzó valores estadísticamente significativos que permita rechazar la hipótesis nula de no tendencia. Nuestro análisis indica también que en una cuenca la temperatura media anual mostró un ligero aumento, mientras que en la otra la temperatura manifestó descendió. Por otro lado, el volumen de agua anual que discurre por las cuencas ha disminuido en ambos ríos donde se pudo rechazar la hipótesis nula de no tendencia con el análisis de Mann-Kendall. No obstante, existen otros factores que no fueron objeto de estudio en este análisis y que tienen impactos sobre los recursos hídricos, así como los distintos mosaicos paisajísticos que componen una cuenca hidrográfica.Palabras Claves: recursos hídricos, tendencias hidroclimáticas, cambio climático.ABSTRACTToday, water resources and climatic oscillations dominate all political and scientific discourses where the principal debate is focused on water management, water pollution and the geopolitics of this resource. Against this background, the IPCC (Intergovernmental Panel for Climate Change), WMO(World Meteorology Organization), the United Nations Environmental Program (UNEP) and other environmental organizations have focused their scientific research on hydro-climatic evolution. In this context, this article examines recent developments in hydro-climatic evolution on two mainrivers in Puerto Rico, using temperature, rainfall, stream-flow and discharge data from NOAA climatic stations and USGS hydrological stations. Initially, we analyze data from each individual station and then make an integrated analysis of the basins. With the obtained results, in none of the basins was it possible to establish clear trends as to total annual precipitation. Though some stations showed positive trends and others regressive, none reached statistically significant values.Our analysis also indicates that in one basin the annual average temperature showed a slight increase, whereas in the other basin a temperature decrease was confirmed. On the other hand, the volume of annual discharge from both rivers showed a decrease. However, there are other factors that were not considered in this analysis, such as land use, that impact water resources and the various landscape mosaics comprising a watershed.Key Words: water resources, hydro-climatic evolution, climate change

APUNTES PARA UN ESTUDIO DE IMPACTO DEL TURISMO EN EL AMBIENTE: EL CASO DE MONTEZUMA, PUNTARENAS

Este artículo desarrolla el diagnostico de los impactos del turismo en el ambiente, en Montezuma, Puntarenas. Se evaluó los efectos de la actividad turísticas en los recursos hídricos, fauna, vegetación y tenencia de la tierra, encontrando que el desarrollo de la actividad, en Montezuma, ha sido sin ninguna planificación y ha venido a alterar la cotidianidad de este pueblo. Aunque se han presentado algunos efectos positivos como son el nacimiento  de una conciencia ambiental entre los diversos grupos de la comunidad, se han desarrollado varios problemas ambientales que están íntimamente ligados a la falta de planificación.Este problema es la alta contaminación del agua, porque no existen sistemas de tratamiento de la misma, además que los antes responsables no supervisan el tratamiento de las aguas tanto potables como servidas en las instalaciones turísticas, las cuales aumentaron aceleradamente durante los últimos cinco años. Por otro lado, se han presentado cambios en el paisaje, han introducido especies exóticas que alteran las condiciones naturales del medio. En cuanto a la tenencia de la tierra en la zona marítima terrestre, ésta se encuentra bajo el dominio de los extranjeros, los costarricenses tienen más números de concesiones pero los extranjeros tienen más superficie (69%). Todos los efectos negativos de turismo en Montezuma, se relaciona con la falta de planificación, por lo que resulta urgente realizar una estrategia de turismo sustentable para el área.ABSTRACTS This article diagnosed the tourism impacts on the environment, in Montezuma, Puntarenas. It e evaluated the effects of tourism in water, wildlife, vegetation and land tenure. It found that tourism in Montezuma have been without planning and it altered the routine life of this town, Even there have been some positive effects like increasing of environmental education, there have been several problems that are very related to the planning lack. Those problems are high water pollution because there is not any system to treat water and also none monitored the water treatment in the hotels, even those increased very fast during the last five years. Changing in the landscape are provoking to loose species even plants or animal, due they have used exotic species in the new hotel. The land tenure in the marine terrestrial zone is controled by foreigners, Costa Ricans have more numbers of concessions but foreigners have more surface (More 69%). Because, all the negative effects of tourism in Montezuma, are related to lack of planning, it is urgent to carry out a management plan for a sustainable tourism in this place.

NOTAS SOBRE CRECIMIENTO DE LA AGLOMERACION DE PUNTARENAS

La ciudad de Puntarenas tiene condiciones apropiadas para constituirse en una aglomeración urbana de cierta envergadura hasta el siglo XXI, más aún si se crean condiciones politicas

GEOGRAFÍA CULTURAL E IDENTIDAD TERRITORIAL: EL CASO DE LA COMUNIDAD DE CABUYA, DISTRITO DE CÓBANO, PUNTARENAS, 2009

El artículo hace referencia a un encuadre teórico desde la geografía cultural, a partir de una sistematización de fuentes bibliográficas seleccionadas, para explicar el análisis espacial a escala local integrando la variable “identidad territorial”. Con tal propósito se elaboró un instrumento que permite identificar dónde es posible señalar rasgos de identidad territorial en la población de Cabuya, con el fin de que la información recopilada sea utilizada como insumo de intervención, en el proceso de participación ciudadana en los talleres que realiza la Universidad Nacional (UNA) para elaborar un plan estratégico en dicha comunidad. ABSTRACT The article refers to a theoretical framework from cultural geography, referring to a bibliographical system of selected sources that explain the spatial analysis to local scale integrating the variable “territorial identity”. With this in mind, we developed a tool to identify, where possible, features of territorial identity in the population of Cabuya so that the compiled information could be used as an input of intervention in the process of citizen participation in workshops conducted by Universidad Nacional to develop a strategic plan for the community of Cabuya.

Mapa de amenazas naturales potenciales: Hoja Puerto Armuelles

Mapa de ubicación de amenazas naturales, con el objetivo de apoyar el proceso de prevención contra desastres naturales en el nivel local.

Mapa de amenazas naturales potenciales: Hoja Puerto Coyote

Mapa de ubicación de amenazas naturales, con el objetivo de apoyar el proceso de prevención contra desastres naturales en el nivel local.

Rates of antimicrobial resistance in latin america (2004-2007) and in vitro activity of the glycylcycline tigecycline and of other antibiotics

As a part of the Tigecycline Evaluation and Surveillance Trial (T.E.S.T.), Gram-positive and Gram-negative bacterial isolates were collected from 33 centers in Latin America (centers in Argentina, Brazil, Chile, Colombia, Guatemala, Honduras, Jamaica, Mexico, Panama, Puerto Rico, and Venezuela) from January 2004 to September 2007. Argentina and Mexico were the greatest contributors of isolates to this study. Susceptibilities were determined according to Clinical Laboratory Standards Institute guidelines. Resistance levels were high for most key organisms across Latin America: 48.3% of Staphylococcus aureus isolates were methicillin-resistant while 21.4% of Acinetobacter spp. isolates were imipenem-resistant. Extended-spectrum β-lactamase were reported in 36.7% of Klebsiella pneumoniae and 20.8% of E. coli isolates. Tigecycline was the most active agent against Gram-positive isolates. Tigecycline was also highly active against all Gram-negative organisms, with the exception of Pseuodomonas aeruginosa, against which piperacillin-tazobactam was the most active agent tested (79.3% of isolates susceptible). The in vitro activity of tigecycline against both Gram-positive and Gram-negative isolates indicates that it may be an useful tool for the treatment of nosocomial infections, even those caused by organisms that are resistant to other antibacterial agents.

Novas espécies de Desmiphorini (Coleoptera, Cerambycidae, Lamiinae) da Costa Rica e do Equador

Novas espécies são descritas: Eupogonius lateralis sp. nov., Estola nigrodorsalis sp. nov., Pseudestoloides rubiginosa sp. nov de Heredia (Costa Rica); Pseudestoloides affinis sp. nov. de Guanacaste and Puntarenas (Costa Rica); Mimopogonius hovorei sp. nov. de Azuay (Equador).

Flow cytometry as a tool in the evaluation of blood leukocyte function in Chelonia mydas (Linnaeus, 1758) (Testudines, Cheloniidae)

Chelonia mydas is a sea turtle that feeds and nests on the Brazilian coast and a disease called fibropapillomatosis is a threat to this species. Because of this, it is extremely necessary to determine a methodology that would enable the analysis of blood leukocyte function in these sea turtles. In order to achieve this aim, blood samples were collected from C. mydas with or without fibropapillomas captured on the São Paulo north coast. Blood samples were placed in tubes containing sodium heparin and were transported under refrigeration to the laboratory in sterile RPMI 1640 cell culture medium. Leukocytes were separated by density gradient using Ficoll-PaqueTM Plus, Amershan Biociences®. The following stimuli were applied in the assessment of leukocyte function: Phorbol Miristate-Acetate (PMA) for oxidative burst activity evaluation and Zymosan A (Saccharomyces cerevisiae) Bio Particles®, Alexa Fluor® 594 conjugate for phagocytosis evaluation. Three cell populations were identified: heterophils, monocytes and lymphocytes. Monocytes were the cells responsible for phagocytosis and oxidative burst.

Genetic diversity of turmeric germplasm (Curcuma longa; Zingiberaceae) identified by microsatellite markers

Turmeric (Curcuma longa) is a triploid, vegetatively propagated crop introduced early during the colonization of Brazil. Turmeric rhizomes are ground into a powder used as a natural dye in the food industry, although recent research suggests a greater potential for the development of drugs and cosmetics. In Brazil, little is known about the genetic variability available for crop improvement. We examined the genetic diversity among turmeric accessions from a Brazilian germplasm collection comprising 39 accessions collected from the States of Goias, Mato Grosso do Sul, Minas Gerais, Sao Paulo, and Para. For comparison, 18 additional genotypes were analyzed, including samples from India and Puerto Rico. Total DNA was extracted from lyophilized leaf tissue and genetic analysis was performed using 17 microsatellite markers (single-sequence repeats). Shannon-Weiner indexes ranged from 0.017 (Minas Gerais) to 0.316 (Sao Paulo). Analyses of molecular variance (AMOVA) demonstrated major differences between countries (63.4%) and that most of the genetic diversity in Brazil is found within states (75.3%). Genotypes from Sao Paulo State were the most divergent and potentially useful for crop improvement. Structure analysis indicated two main groups of accessions. These results can help target future collecting efforts for introduction of new materials needed to develop more productive and better adapted cultivars.

Evaluation of the carbon content of aerosols from the burning of biomass in the Brazilian Amazon using thermal, optical and thermal-optical analysis methods

Aerosol samples were collected at a pasture site in the Amazon Basin as part of the project LBA-SMOCC-2002 (Large-Scale Biosphere-Atmosphere Experiment in Amazonia - Smoke Aerosols, Clouds, Rainfall and Climate: Aerosols from Biomass Burning Perturb Global and Regional Climate). Sampling was conducted during the late dry season, when the aerosol composition was dominated by biomass burning emissions, especially in the submicron fraction. A 13-stage Dekati low-pressure impactor (DLPI) was used to collect particles with nominal aerodynamic diameters (D(p)) ranging from 0.03 to 0.10 mu m. Gravimetric analyses of the DLPI substrates and filters were performed to obtain aerosol mass concentrations. The concentrations of total, apparent elemental, and organic carbon (TC, EC(a), and OC) were determined using thermal and thermal-optical analysis (TOA) methods. A light transmission method (LTM) was used to determine the concentration of equivalent black carbon (BC(e)) or the absorbing fraction at 880 nm for the size-resolved samples. During the dry period, due to the pervasive presence of fires in the region upwind of the sampling site, concentrations of fine aerosols (D(p) < 2.5 mu m: average 59.8 mu g m(-3)) were higher than coarse aerosols (D(p) > 2.5 mu m: 4.1 mu g m(-3)). Carbonaceous matter, estimated as the sum of the particulate organic matter (i.e., OC x 1.8) plus BC(e), comprised more than 90% to the total aerosol mass. Concentrations of EC(a) (estimated by thermal analysis with a correction for charring) and BC(e) (estimated by LTM) averaged 5.2 +/- 1.3 and 3.1 +/- 0.8 mu g m(-3), respectively. The determination of EC was improved by extracting water-soluble organic material from the samples, which reduced the average light absorption Angstrom exponent of particles in the size range of 0.1 to 1.0 mu m from >2.0 to approximately 1.2. The size-resolved BC(e) measured by the LTM showed a clear maximum between 0.4 and 0.6 mu m in diameter. The concentrations of OC and BC(e) varied diurnally during the dry period, and this variation is related to diurnal changes in boundary layer thickness and in fire frequency.

Luminescent Mycena: new and noteworthy species

Seven species or Mycena are reported as luminescent, representing specimens collected in Belize, Brazil, Dominican Republic, Jamaica, japan (Bonin Islands), Malaysia (Borneo) and Puerto Rico. Four of them represent new species (Mycena luxaeterna, M. luxarboricola, M. luxperpetua, M. silvaelucens) and three represent new reports of luminescence in previously described species (M. aff. abieticola, M. aspratilis, M. margarita). Mycena subepipterygia is synonymized with M. margarita, and M. chlorinosma is proposed as a possible synonym. Comprehensive descriptions, illustrations, photographs and comparisons with phenetically similar species are provided. A redescription of M. chlorophos, based on analyses of type specimens and recently collected topotypical material, is provided. The addition of these seven new or new, reported luminescent species of Mycena brings the total to 71 known bioluminescent species of fungi.

Forest structure and live aboveground biomass variation along an elevational gradient of tropical Atlantic moist forest (Brazil)

Live aboveground biomass (AGB) is an important source of uncertainty in the carbon balance from the tropical regions in part due scarcity of reliable estimates of live AGB and its variation across landscapes and forest types. Studies of forest structure and biomass stocks of Neotropical forests are biased toward Amazonian and Central American sites. In particular, standardized estimates of aboveground biomass stocks for the Brazilian Atlantic forest are rarely available. Notwithstanding the role of environmental variables that control the distribution and abundance of biomass in tropical lowland forests has been the subject of considerable research, the effect of short, steep elevational gradients on tropical forest structure and carbon dynamics is not well known. In order to evaluate forest structure and live AGB variation along an elevational gradient (0-1100 m a.s.l.) of coastal Atlantic Forest in SE Brazil, we carried out a standard census of woody stems >= 4.8 cm dbh in 13 1-ha permanent plots established on four different sites in 2006-2007. Live AGB ranged from 166.3 Mg ha(-1) (bootstrapped 95% CI: 1444,187.0) to 283.2 Mg ha(-1) (bootstrapped 95% CI: 253.0,325.2) and increased with elevation. We found that local-scale topographic variation associated with elevation influences the distribution of trees >50 cm dbh and total live AGB. Across all elevations, we found more stems (64-75%) with limited crown illumination but the largest proportion of the live AGB (68-85%) was stored in stems with highly illuminated or fully exposed crowns. Topography, disturbance and associated changes in light and nutrient supply probably control biomass distribution along this short but representative elevational gradient. Our findings also showed that intact Atlantic forest sites stored substantial amounts of carbon aboveground. The live tree AGB of the stands was found to be lower than Central Amazonian forests, but within the range of Neotropical forests, in particular when compared to Central American forests. Our comparative data suggests that differences in live tree AGB among Neotropical forests are probably related to the heterogeneous distribution of large and medium-sized diameter trees within forests and how the live biomass is partitioned among those size classes, in accordance with general trends found by previous studies. In addition, the elevational variation in live AGB stocks suggests a large spatial variability over coastal Atlantic forests in Brazil, clearly indicating that it is important to consider regional differences in biomass stocks for evaluating the role of this threatened tropical biome in the global carbon cycle. (C) 2010 Elsevier B.V. All rights reserved.