Jornalismo de portal: análise de conteúdo do G1 nas áreas de cobertura da TV Tem

This work aims to situate the main conceptual aspects of regional and local media and draw a brief overview of current trends in online journalism, especially practiced by TV TEM, an affiliate of Rede Globo in São Paulo. The methodology of the study is based on literature and documents, as well as systematic analysis of G1 portals, TV HAS administered by the regional districts of Bauru, Itapetininga, São José do Rio Preto and Sorocaba. Data were collected through interviews with managers, editors, reporters and some interns. As a result, we observed the news-value criteria, selection and management of the responsible gatewatching adopted by major portals Globo.com homes and G1

Comunicação pública e democracia digital nos processos de participação cidadã: uma análise do Portal Brasil, sob os princípios de E-engagement

This article conducted an analysis of Portal Brazil, specifically in the areas of “Education” and “Economy and Employment”, by according to the principles of e-engagement defined by the Organization for Economic Co- operation and Development (OECD). For understanding of this work, were elucidated concepts such as public communication - trajectory, principles and relations -, digital democracy, e-government and engagement and civic participation. Through this analysis was realized the importance of public communication in the processes of opinion formation and stimulus to citizen participation, as well as the performance of the internet as a facilitator instrument in the processes of relationship between State and Society

Estudo de caso do conforto térmico de dois modelos construtivos em madeira: wood frame e log home

O presente trabalho consiste em demonstrar valores referentes ao conforto ambiental de dois modelos construtivos, sendo o primeiro um edifício já executado e o outro em fase de implantação, ambos no Campus Experimental de Itapeva, aplicando alguns conceitos em habitação mais sustentáveis de madeira na região de Itapeva, estado de São Paulo, Brasil. A edificação construída sedia duas salas de aula a partir da tipologia construtiva wood frame, constituída basicamente de paredes autoportantes compostas de ossatura de madeira tratada de pinus.sp, chapas estruturais em OSB, manta de isolamento térmico/acústico e fechamento interno e externo, respectivamente em gesso e sidding (lambris de madeira). A habitação em fase de início de implantação foi idealizada a partir da tipologia construtiva log-home, e atenderá a necessidade de espaço físico para abrigar o Centro Acadêmico e a empresa-júnior “PROMAD”, ambos do curso de Engenharia Industrial Madeireira do Campus Experimental da UNESP/Itapeva. Esta construção foi elaborada a partir de técnicas de baixo impacto ambiental, levando em consideração os resultados de análise térmica obtidos na edificação anterior. Em ambos modelos construtivos foram empregados recursos renováveis abundantes na região, como a madeira de reflorestamento de Pinus e de Eucalipto. Foi acrescentado ainda ao segundo modelo a utilização de resíduos industriais lignocelulósicos, como rolete de laminação e outros materiais locais disponíveis. A metodologia básica empregada para a obtenção de dados sobre o conforto térmico em relação as edificações foi, para o primeiro caso, a coleta de dados de temperatura e umidade utilizando um termo-higrômetro, a sistematização e análise de dados reais obtidos no interior da edificação. No segundo caso, o dimensionamento do conforto térmico... (Resumo completo, clicar acesso eletrônico abaixo)

A construção da notícia no portal choike.org: comunicação alternativa na internet

This project aims to study the influence of information technology in the construction of new forms of citizenship. A large space as possible by the Internet allows the emergence of alternative news sources, as opposed to the means of mass communication. The research will take from the study of the news portal Choike.org. Maintained by civil society representatives, the website has a proposal to address topics that are infrequent in the media hegemony. To facilitate the study, we established a cut. Let's look at five bulletins Choike.org portal between the years 2005 and 2010 that address the issue of agrarian reform in Latin America, especially in Brazil. The issue is discussed by the media in general, however has more emphasis between the means of alternative journalism. For Choike.org portal, agrarian reform this intrinsically linked to social development. Therefore, this analysis seeks to highlight the role of new technologies as an important tool to aid discussion of the issue in the media as having specific focus to the issue of land reform

Análise descritiva da construção histórico-social do olhar de psicologia sobre a homessexualidade a partir de produções do portal de periódicos Pepsi: um estudo bibliográfico

Pós-graduação em Educação Sexual - FCLAR

Portal único (SILSTM) para ovário-histerectomia vídeo-assistida em cadelas

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Morphometric evaluation by ultrasonographic exam of the portal veinm vaudal vein and abdominal aorta in healthy dogs of diferent body weights

The diameters and areas of portal vein, caudal vena cava and abdominal aorta are useful measurements in dogs. These values can be easily measured by ultrasonographic exam, and variations of normality can be an important indicator of hepatic or extra-hepatic alterations. This study aimed to measure the diameter and areas of portal vein, caudal vena cava and abdominal aorta inhealthy dogs, with normal corporal score, divided in groups according to the body weight, and assess whether the data are influenced by animal weight. Thirty dogs were examined and divided into three groups (Group A: ≤ 10 kg Group B: from 10.1 to 20.0 kg; Group C: ≥ 20.1 kg). To measure thediameters and areas of portal vein, caudal vena cava and abdominal aorta, the animal was kept in left lateral decubitus position and the transducer was placed on the right lateral abdominal wall, at approximately the 10th or 11th intercostal space, in the porta hepatis region. The diameters and areas of the portal vein, caudal vena cava and abdominal aorta were significantly lower for dogs in Group A with respect to other groups and the dogs from Groups B and C had similar results with each other. The diameters and areas of the portal vein, caudal vena cava and abdominal aorta may vary with the animal size, and reference values must be specific for small, medium and large dogs. Key words: abdominal vessels; area; diameter; measurement; ultrasonographic exam

A educação sexual no portal do professor-MEC: Estudo analítico-descritivo das temáticas referentes à sexualidade no espaço da aula

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Endothelium dependent expression and underlying mechanisms of des-Arg(9)-bradykinin-induced B1R-mediated vasoconstriction in rat portal vein

Endothelial dysfunction has been implicated in portal vein obstruction, a condition responsible for major complications in chronic portal hypertension. Increased vascular tone due to disruption of endothelial function has been associated with an imbalance in the equilibrium between endothelium-derived relaxing and contracting factors. Herein, we assessed underlying mechanisms by which expression of bradykinin B-1 receptor (B1R) is induced in the endothelium and how its stimulation triggers vasoconstriction in the rat portal vein. Prolonged in vitro incubation of portal vein resulted in time- and endothelium-dependent expression of B1R and cyclooxygenase-2 (COX-2). Inhibition of protein kinase C (PKC) or phosphatidylinositol 3-kinase (PI3K) significantly reduced expression of B1R through the regulation of transcription factors, activator protein-1 (AP-1) and cAMP response element-binding protein (CREB). Moreover, pharmacological studies showed that B1R-mediated portal vein contraction was reduced by COX-2, but not COX-1, inhibitors. Notably, activation of endothelial B1R increased phospholipase A(2)/COX-2-derived thromboxane A(2) (TXA(2)) levels, which in turn mediated portal vein contraction through binding to TXA(2) receptors expressed in vascular smooth muscle cells. These results provide novel molecular mechanisms involved in the regulation of B1R expression and identify a critical role for the endothelial B1R in the modulation of portal vein vascular tone. Our study suggests a potential role for B1R antagonists as therapeutic tools for diseases where portal hypertension may be involved. (C) 2012 Elsevier Inc. All rights reserved.

Effects of the administration of pentoxifylline and prednisolone on the evolution of portal fibrogenesis secondary to biliary obstruction in growing animals: immunohistochemical analysis of the expression of TGF-BETA; and VEGF

OBJECTIVE: During the neonatal and infancy periods, some chronic liver diseases may lead to progressive hepatic fibrosis, which is a condition that can ultimately result in the loss of organ function and severe portal hypertension necessitating hepatic transplantation. In a previous report, pharmacological interventions were demonstrated to modulate hepatic fibrosis induced by bile duct ligation in young rats. The administration of pentoxifylline or prednisolone, or the combination of both, resulted in reduced fibrogenesis in portal spaces. The objectives of the present study were to evaluate the expression of transforming growth factor beta and vascular endothelial growth factor after bile duct ligation in young rats and to assess the effect of those same drugs on cytokine expression. METHODS: In this experimental study, 80 young rats (21 or 22 days old) were submitted either to laparotomy and common bile duct ligation or to sham surgery. The animals were allocated into four groups according to surgical procedure, and the following treatments were administered: (1) common bile duct ligation + distilled water, (2) sham surgery + distilled water, (3) common bile duct ligation + pentoxifylline, or (4) common bile duct ligation + prednisolone. After 30 days, a hepatic fragment was collected from each animal for immunohistochemical analysis using monoclonal antibodies against transforming growth factor beta and vascular endothelial growth factor. Digital morphometric and statistical analyses were performed. RESULTS: The administration of pentoxifylline reduced the transforming growth factor beta-marked area and the amount of transforming growth factor beta expressed in liver tissue. This effect was not observed after the administration of prednisolone. There was a significant reduction in vascular endothelial growth factor expression after the administration of either drug compared with the non-treatment group. CONCLUSIONS: The administration of pentoxifylline to cholestatic young rats resulted in the diminished expression of transforming growth factor beta and vascular endothelial growth factor in liver tissue. The administration of steroids resulted in the diminished expression of vascular endothelial growth factor only. These pathways may be involved in hepatic fibrogenesis in young rats submitted to bile duct ligation and exposed to pentoxifylline or prednisolone.

A new portal for the analysis of human splicing variants

Understanding alternative splicing is crucial to elucidate the mechanisms behind several biological phenomena, including diseases. The huge amount of expressed sequences available nowadays represents an opportunity and a challenge to catalog and display alternative splicing events (ASEs). Although several groups have faced this challenge with relative success, we still lack a computational tool that uses a simple and straightforward method to retrieve, name and present ASEs. Here we present SPLOOCE, a portal for the analysis of human splicing variants. SPLOOCE uses a method based on regular expressions for retrieval of ASEs. We propose a simple syntax that is able to capture the complexity of ASEs.

Modeling and Identification of an Open-frame Underwater Vehicle: The Yaw Motion Dynamics

A semi-autonomous unmanned underwater vehicle (UUV), named LAURS, is being developed at the Laboratory of Sensors and Actuators at the University of Sao Paulo. The vehicle has been designed to provide inspection and intervention capabilities in specific missions of deep water oil fields. In this work, a method of modeling and identification of yaw motion dynamic system model of an open-frame underwater vehicle is presented. Using an on-board low cost magnetic compass sensor the method is based on the utilization of an uncoupled 1-DOF (degree of freedom) dynamic system equation and the application of the integral method which is the classical least squares algorithm applied to the integral form of the dynamic system equations. Experimental trials with the actual vehicle have been performed in a test tank and diving pool. During these experiments, thrusters responsible for yaw motion are driven by sinusoidal voltage signal profiles. An assessment of the feasibility of the method reveals that estimated dynamic system models are more reliable when considering slow and small sinusoidal voltage signal profiles, i.e. with larger periods and with relatively small amplitude and offset.

Influence of HAART on Alternative Reading Frame Immune Responses over the Course of HIV-1 Infection

Background: Translational errors can result in bypassing of the main viral protein reading frames and the production of alternate reading frame (ARF) or cryptic peptides. Within HIV, there are many such ARFs in both sense and the antisense directions of transcription. These ARFs have the potential to generate immunogenic peptides called cryptic epitopes (CE). Both antiretroviral drug therapy and the immune system exert a mutational pressure on HIV-1. Immune pressure exerted by ARF CD8(+) T cells on the virus has already been observed in vitro. HAART has also been described to select HIV-1 variants for drug escape mutations. Since the mutational pressure exerted on one location of the HIV-1 genome can potentially affect the 3 reading frames, we hypothesized that ARF responses would be affected by this drug pressure in vivo. Methodology/Principal findings: In this study we identified new ARFs derived from sense and antisense transcription of HIV-1. Many of these ARFs are detectable in circulating viral proteins. They are predominantly found in the HIV-1 env nucleotide region. We measured T cell responses to 199 HIV-1 CE encoded within 13 sense and 34 antisense HIV-1 ARFs. We were able to observe that these ARF responses are more frequent and of greater magnitude in chronically infected individuals compared to acutely infected patients, and in patients on HAART, the breadth of ARF responses increased. Conclusions/Significance: These results have implications for vaccine design and unveil the existence of potential new epitopes that could be included as vaccine targets.

The use of Open Reading frame ESTs (ORESTES) for analysis of the honey bee transcriptome

Abstract Background The ongoing efforts to sequence the honey bee genome require additional initiatives to define its transcriptome. Towards this end, we employed the Open Reading frame ESTs (ORESTES) strategy to generate profiles for the life cycle of Apis mellifera workers. Results Of the 5,021 ORESTES, 35.2% matched with previously deposited Apis ESTs. The analysis of the remaining sequences defined a set of putative orthologs whose majority had their best-match hits with Anopheles and Drosophila genes. CAP3 assembly of the Apis ORESTES with the already existing 15,500 Apis ESTs generated 3,408 contigs. BLASTX comparison of these contigs with protein sets of organisms representing distinct phylogenetic clades revealed a total of 1,629 contigs that Apis mellifera shares with different taxa. Most (41%) represent genes that are in common to all taxa, another 21% are shared between metazoans (Bilateria), and 16% are shared only within the Insecta clade. A set of 23 putative genes presented a best match with human genes, many of which encode factors related to cell signaling/signal transduction. 1,779 contigs (52%) did not match any known sequence. Applying a correction factor deduced from a parallel analysis performed with Drosophila melanogaster ORESTES, we estimate that approximately half of these no-match ESTs contigs (22%) should represent Apis-specific genes. Conclusions The versatile and cost-efficient ORESTES approach produced minilibraries for honey bee life cycle stages. Such information on central gene regions contributes to genome annotation and also lends itself to cross-transcriptome comparisons to reveal evolutionary trends in insect genomes.