DnaG interacts with a linker region that joins the N- and C-domains of DnaB and induces the formation of 3-fold symmetric rings

Loading of the replicative ring helicase onto the origin of replication (oriC) is the final outcome of a well coordinated series of events that collectively constitute a primosomal cascade. Once the ring helicase is loaded, it recruits the primase and signals the switch to the polymerization mode. The transient nature of the helicase-primase (DnaB-DnaG) interaction in the Escherichia coli system has hindered our efforts to elucidate its structure and function. Taking advantage of the stable DnaB-DnaG complex in Bacillus stearothermophilus, we have reviewed conflicting mutagenic data from other bacterial systems and shown that DnaG interacts with the flexible linker that connects the N- and C-terminal domains of DnaB. Furthermore, atomic force microscopy (AFM) imaging experiments show that binding of the primase to the helicase induces predominantly a 3-fold symmetric morphology to the hexameric ring. Overall, three DnaG molecules appear to interact with the hexameric ring helicase but a small number of complexes with two and even one DnaG molecule bound to DnaB were also detected. The structural/functional significance of these data is discussed and a speculative structural model for this complex is suggested.

Inclusão de crianças especiais em uma escola pública de Ribeira do Pombal, Bahia, Brasil : um estudo de caso

A inclusão de crianças com necessidades especiais nas escolas comuns é uma idealização voltada para a oportunidade e o respeito à diversidade. Com a Constituição Federal Brasileira (1988), no seu capítulo III, da Educação, da cultura e do desporto, Art. 205, rege que ―A educação, direito de todos e dever do Estado e da família [...]‖ denota que lugar de criança é na escola, independente de credo, deficiência, raça e cultura. O presente estudo de caso na escola pública Rui Barbosa, situada na cidade de Ribeira do Pombal, Bahia, Brasil, pretende conhecer a práxis educativa e o perfil dos profissionais sobre a proposta e a prática pedagógica inclusiva, como também analisar o espaço físico, observando se há ou não barreiras arquitetônicas e acessibilidade para os alunos com deficiência e também a participação da família, em inserir o filho no ambiente formal. Esta investigação tem como base a revisão da literatura sobre inclusão, com a utilização, no enquadramento teórico, da legislação brasileira, entre outros autores voltados ao tema. O desenvolvimento do estudo possui característica qualitativa, através de observação, questionário, entrevista, visitas, ou seja, uma construção através do diálogo e vivência diária realizada no turno matutino. Os participantes dessa investigação foram: gestores, coordenadora da Educação Especial e coordenadora do ensino comum, professores, agentes administrativos, representante da secretaria municipal, profissional da saúde (psicóloga), famílias e alunos do ensino comum e especial. A análise dos resultados obtidos revela que a proposta inclusiva encontra-se em processo de evolução humana e arquitetônica, mas também regista-se pontos positivos no aumento de número de matrículas, ou seja, os pais estão sendo conquistados e adquirindo confiança na instituição. Os professores, apesar da insegurança, da carência de auxílio, não tratam os alunos com desdém e buscam auxiliá-los como podem e sabem. Por fim, a escola aposta num trabalho que envolva o interpessoal e intrapessoal em busca da inclusão sem obstáculos.

Uso do bivalve límnico Anodontites tenebricosus (LEA, 1834) no biomonitoramento de metais do Rio Ribeira de Iguape

This study investigated the contamination of the Ribeira de Iguape River - RIR by Cd, Zn, Cr and Pb, using the bivalve Anodontites tenebricosus as a biomonitor. Metal concentrations in tissue samples were measured by HR-ICPMS. Bivalve tissues exhibited mean levels of 1.00 µg/g Cd; 152.89 µg/g Zn; 14.79 µg/g Cr and 4.40 µg/g Pb. Lead concentrations were comparable to those reported for moderately contaminated sites. The results showed that Pb is bioavailable to the bivalves, exhibiting high concentrations and exceeding both natural and reference values for human consumption. The freshwater bivalve Anodontites tenebricosus is a suitable biomonitor of contamination by metals.

Revitalização urbana de centros históricos: uma revisão de contextos e propostas: a Ribeira como estudo de caso

This dissertation focuses on the theme of urban revitalization in historical centers. It starts from a survey of international and national concepts and experiences about the subject, in special, the historical center of Ribeira, in Natal, Brazil. It gives more attention to local management and it is dedicated to the study of objectives and actions developed, basically, by two segments that manage works in the district. Companhia Docas do Rio Grande do Norte aims at the economical development of the state and it implies the enlargement of capacity of loading and unloading of the harbor of Natal and the Natal city counsil aims at urban preservation and revitalization. It was analysed speeches ans projects related to Ribeira and it was discovered that Natal has done very little to revitalize its historical center in the last two dacades. It analyses what seemed, or at least, should be a conflict of interests between incompatible projects, revealing both conciliatory proceedings and connivance between the participants in decision making. The conclusion directs attentions to the threat to historical building in the city and the importance of enhancing discussion about the future of our historical sites

Turismo cultural na ZEPH-Ribeira: possibilidades e limitações

Considering that urban interventions, planned and executed recently at Natal, RN, should put Ribeira - one of the town centers - in the new limits of the expanded center of the city, this work examines the use of this new accessibility for the benefit of the development of an infrastructure to support a type of tourism currently seen as an alternative to diversification into areas dominated by entertainment tourism (the case of Natal). A local with city's traditional cultural facilities and holder of a set built that is a synthesis of the images of the neighborhood, it fears that this increase in the potential for movement and flows of use stimulate speculation and deployment of a bulk type of activity incompatible with the preservation the architectural heritage. Important economy for the city, tourism has been showing a factor propellant processing urban related changes in accessibility. It is argued that cultural tourism, often used in plans and projects of revitalization across the country, may be factor for sustainable development and conserve the assets, and contributes to the process of revitalization of the historic cultural center as Ribeira. Intended to illustrate the need to consider other information that might help in the revitalization of the neighborhood, this research presents the offer cultural tourism of Ribeira and identifies and maps certain physical attributes that, combined with functional attributes, can stimulate or inhibit the diversity and vitality in the region

Ribeira, técnica versus natureza: transformações em prol de um projeto de modernização (1860-1932)

The necessity of the insertion of the capital of Rio Grande do Norte in the world-wide commercial scene and its claim as the seat of political power, in ends of nineteenth and beginning of twentieth century, determined the direction of urban interventions undertaken by government to restructure the city. In that matter, there were several actions of improvements and embellishment in Natal, which had, as a starting point, the adequacy works of the port, located in the Ribeira quarter, with the aim of ending the physical isolation that reinforced its economic stagnation. Besides the problems faced in the opening bar of the Potengi River, and would complement the required improvements, other barriers demonstrate the tension established between the physic-geographic field and the man: the flooded and slope which connected Cidade Alta and Ribeira the first two quarters of the city.The execution of these works demanded knowledge whose domain and application it was for engineering. But, how the actions done for the engineers, in sense to transform natural areas into constructed spaces made possible the intentional conformation of the quarter of the Ribeira in a commercial and politician-administrative center, in the middle of the XIX century and beginning of the XX? Understand, therefore, the employment effects of technology on the physical-geographical Ribeira, is the objective of this work that uses theoretical and methodological procedures of Urban Environmental History, by analyzing the relationship between the environment and the man, mediated by knowledge and use of technologies. The documental research was used, as primary sources, the Messages of the Provincial Assembly Government that later became the Legislative Assembly of Rio Grande do Norte reports and articles on specialized publications, in addition to local newspapers. The work is structured in five chapters. First, some comments about Urban Environmental History (Chapter 1) supplemented with analysis of the conceptual construction of nature in the Contemporary Era and its application in the city (chapter 02), the following chapters (03 and 04) deal with the rise of engineers as a active group in the Brazilian government frameworks and their vision about the nature inside the urban environment and it is studied how the professional technicians dealt with the improvement work of the harbor and in the shock with the natural forces. Other works that would complement this "project" of modernization and had had natural obstacles to be removed the Ribeira flood and slope constitute the subject of the fifth chapter. Finally, some final considerations retake the initial discussions aiming an association between the technique and the nature as junction elements inside the process of constitution of a Modern Natal

Intervenção em linhas de água com recurso a técnicas de Engenharia Natural. Caso de estudo do Parque Urbano da Ribeira de Porto Salvo em Oeiras

Mestrado em Arquitectura Paisagista - Instituto Superior de Agronomia - UL

Percepção ambiental do patrimônio cultural: estudo de caso na Cidade Alta e Ribeira em Natal-RN

Contemporary studies have shown that the evolution of the heritage concepts is accompanied by an affirmation of the importance of social participation in recognizing heritage values and in managing cultural assets. We used the Brazilian context to emphasize the challenges for democratizing this process. This problematic is discussed based on the cases of Cidade Altaand Ribeira, neighborhoods that date from the formation of Natal-RNand have cultural assets recognized by levels of government. The study builds elements to answer the research question: what meanings and representations does the culturalheritage in the case study have for its users? The research method analyzes the representations and the meanings of the neighborhoods, firstly is based on historiographical studies, memories records of the city and on the process of heritage management. Secondly, it isbased on the field research, it is structured in environmental perception studies (areas of Environmental Psychology, Architecture and Urbanism) and has been applied with users with different bonds with the studied environment (residents, workers and visitors). The data were obtained with the multi-method which included direct observation, questionnaire survey and mentalmaps (that replicate Kevin Lynch). The analysis of result verified the research hypothesis, emphasizing aspects of the relationship between users and cultural heritage relevant to strengthening collective memory, local identity, contributing to heritage management. Among the results, the socio-environmental image obtained which emphasized a "cultural axis" linkingboth studied neighborhoods and confirms the influences of elements rein the memories records of the city and in the area s management. Identified aspects to strengthen the relationship between the users and cultural assets, such as the presence of placeswith affective ties to certain groups, as well as the need to fight off negative images (of degradation and insecurity) associated to the site and also expand the participation of the population, including residents, in policies and cultural activities. After all, recognition of value and the involvement of societycultural assets have the potential of contribute to integrate city development with heritage conservation

Reutilização de imóveis de interesse patrimonial, voltados para a ahabitação: um estudo de caso na Ribeira-Natal/RN

This work analyzes the reutilization of real estate of patrimonial interest, come back toward habitation. One understands as real estate of patrimonial interest the old ones that present relevant architectural typologies that must be preserved; some buildings that represent characteristic architectural styles of a determined period; some valued real estates for the history of the city (historical, memory and image signification that has of certain places) and buildings with exceptional artistic elements. In short, real estate of patrimonial interest. The place or site to be studied will be the neighborhood of the Ribeira, because is an area that protects a bigger interest for the historic patrimony of the city. The habitation use was thought as a booster element of revitalization processes of degradable historic site, contributing to the preservation of artistic, architectural and historic patrimony, and thus to stimulate the accomplishment of the re-qualification of the neighborhood of the Ribeira. It is intended with the present study to examine alternatives to make possible habitation re (uses) in the historical quarter of the Ribeira in old constructions of patrimonial value

Green Belt 7 Bridges: Phase One, Context, Structure, Form

OTRI 1508 " GREEN BELT 7 BRIDGES"

Iowa Seat Belt Use Survey 2013 Data Collection Methodology Report, September 26, 2013

In an effort to achieve greater consistency and comparability in state-wide seat belt use reporting, the National Highway Traffic Safety Administration (NHTSA) issued new requirements in 2011 for observing and reporting future seat belt use. The requirements included the involvement of a qualified statistician in the sampling and weighting portions of the process as well as a variety of operational details. The Iowa Governor’s Traffic Safety Bureau contracted with Iowa State University’s Survey & Behavioral Research Services (SBRS) in 2011 to develop the study design and data collection plan for the State of Iowa annual survey that would meet the new requirements of the NHTSA. A seat belt survey plan for Iowa was developed by SBRS with statistical expertise provided by Zhengyuan Zhu, Ph.D., Associate Professor of Statistics at Iowa State University and was approved by NHTSA on March 19, 2012.

Iowa Seat Belt Use Survey 2014 Data Collection Methodology Report, September 25, 2014

In an effort to achieve greater consistency and comparability in state-wide seat belt use reporting, the National Highway Traffic Safety Administration (NHTSA) issued new requirements in 2011 for observing and reporting future seat belt use. The requirements included the involvement of a qualified statistician in the sampling and weighting portions of the process as well as a variety of operational details. The Iowa Governor’s Traffic Safety Bureau contracted with Iowa State University’s Survey & Behavioral Research Services (SBRS) in 2011 to develop the study design and data collection plan for the State of Iowa annual survey that would meet the new requirements of the NHTSA. A seat belt survey plan for Iowa was developed by SBRS with statistical expertise provided by Zhengyuan Zhu, Ph.D., Associate Professor of Statistics at Iowa State University and was approved by NHTSA on March 19, 2012.

Iowa Seat Belt Use Survey 2015 Data Collection Methodology Report, September 9, 2015

In an effort to achieve greater consistency and comparability in state-wide seat belt use reporting, the National Highway Traffic Safety Administration (NHTSA) issued new requirements in 2011 for observing and reporting future seat belt use. The requirements included the involvement of a qualified statistician in the sampling and weighting portions of the process as well as a variety of operational details. The Iowa Governor’s Traffic Safety Bureau contracted with Iowa State University’s Survey & Behavioral Research Services (SBRS) in 2011 to develop the study design and data collection plan for the State of Iowa annual survey that would meet the new requirements of the NHTSA. A seat belt survey plan for Iowa was developed by SBRS with statistical expertise provided by Zhengyuan Zhu, Ph.D., Associate Professor of Statistics at Iowa State University and Director of the Center for Survey Statistics and Methodology. The plan was approved by NHTSA on March 19, 2012.

Nitrogen and potassium fertilization on 'Caipira' and 'BRS Princesa' bananas in the Ribeira Valley.

2016

Utilização de sistemas agroflorestais para a restauração de matas ciliares no quilombo Ivaporunduva, no vale do Ribeira/SP.

2008