981 resultados para Reference architecture


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[ES]El proyecto presentado a continuación muestra la elaboración de un core para ser embebido dentro de las denominadas FPGAs (Field Programmable Gate Array), cuya finalidad es la creación de una referencia temporal, en arquitectura de 64bits, gracias a un módulo GPS (Global Positioning System), lo más cercana posible al orden de las decenas de nano-segundos, para poder ser insertado en un equipo PTP-Master (Precision Time Protocol - Master) (IEEE (Institute of Electrical and Electronics Engineers) - 1588), a bajo coste y con calidad comparable a la de los dispositivos Grand Master.

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Interleukin 2 (IL2) is the primary growth hormone used by mature T cells and this lymphokine plays an important role in the magnification of cell-mediated immune responses. Under normal circumstances its expression is limited to antigen-activated type 1 helper T cells (TH1) and the ability to transcribe this gene is often regarded as evidence for commitment to this developmental lineage. There is, however, abundant evidence than many non-TH1 T cells, under appropriate conditions, possess the ability to express this gene. Of paramount interest in the study of T-cell development is the mechanisms by which differentiating thymocytes are endowed with particular combinations of cell surface proteins and response repertoires. For example, why do most helper T cells express the CD4 differentiation antigen?

As a first step in understanding these developmental processes the gene encoding IL2 was isolated from a mouse genomic library by probing with a conspecific IL2 cDNA. The sequence of the 5' flanking region from + 1 to -2800 was determined and compared to the previously reported human sequence. Extensive identity exists between +1 and -580 (86%) and sites previously shown to be crucial for the proper expression of the human gene are well conserved in both sequence location in the mouse counterpart.

Transient expression assays were used to evaluate the contribution of various genomic sequences to high-level gene expression mediated by a cloned IL2 promoter fragment. Differing lengths of 5' flanking DNA, all terminating in the 5' untranslated region, were linked to a reporter gene, bacterial chloramphenicol acetyltransferase (CAT) and enzyme activity was measured after introduction into IL2-producing cell lines. No CAT was ever detected without stimulation of the recipient cells. A cloned promoter fragment containing only 321 bp of upstream DNA was expressed well in both Jurkat and EL4.El cells. Addition of intragenic or downstream DNA to these 5' IL2-CAT constructs showed that no obvious regulatory regions resided there. However, increasing the extent of 5' DNA from -321 to -2800 revealed several positive and negative regulatory elements. One negative region that was well characterized resided between -750 and -1000 and consisted almost exclusively of alternating purine and pyrimidines. There is no sequence resembling this in the human gene now, but there is evidence that there may have once been.

No region, when deleted, could relax either the stringent induction-dependence on cell-type specificity displayed by this promoter. Reagents that modulated endogenous IL2 expression, such as cAMP, cyclosporin A, and IL1, affected expression of the 5' IL2-CAT constructs also. For a given reagent, expression from all expressible constructs was suppressed or enhanced to the same extent. This suggests that these modulators affect IL2 expression through perturbation of a central inductive signal rather than by summation of the effects of discrete, independently regulated, negative and positive transcription factors.

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This paper tries to develop more generally some fundamental bases for the ecological study of freshwater plankton. A special attention is given to the phytoplankton associations which can be separated out and made into groups according to their dependence upon changing environments. Plankton formations in different types of water bodies (ponds, lakes and rivers) are studied.

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Members of the family Gammaridae are very closely interrelated. There arises the question as to how far they also differ amongst themselves through physiological characteristics. Comparative respiratory and physiological experiments were made on the five euryhaline species Gammarus locusta, G. oceanicus, G. salinus, G. zaddachi and G. duebeni. The respiratory measurements carried out within the framework of this experiment were occupied with the relationships between oxygen consumption and body size depending on salinity. They also had the object of determing the variations in metabolic intensity after an abrupt change in the salt content of the external medium, and to establish the period of time for the process of adaptation. As the experiments were carried out polarographically in a testing plant with continuous flow-through, and the method which was applied permitted continuous recording over prolonged intervals, there could also be carried out comparisons between metabolism at rest and under activity, and the alterations of oxygen consumption during the process of moulting could be measured.

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A visual pattern recognition network and its training algorithm are proposed. The network constructed of a one-layer morphology network and a two-layer modified Hamming net. This visual network can implement invariant pattern recognition with respect to image translation and size projection. After supervised learning takes place, the visual network extracts image features and classifies patterns much the same as living beings do. Moreover we set up its optoelectronic architecture for real-time pattern recognition. (C) 1996 Optical Society of America

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Abstract Environmental changes may have an impact on life conditions of the fish, e.g. food supply for the fish. The prevailing environmental conditions apply evenly to all age groups of one stock. Small fish have high growth rates, whereas large fish grow with low rates. But, it can be shown on the basis of the von Bertalanffy-growth model that it is sufficient to know only the growth rate of one single age group to compute the growth rates of all other age groups. The growth rate of a reference fish GRF (e.g. a fish with a body mass of 1 kg) was introduced as a reference growth describing the current food condition of all age groups of the stock. As an example a time series of the reference-growth rate of the northern cod stock (NAFO, 3K) was computed for the time span 1979 to 1999. For the northern cod stock it can be observed that environmental conditions caused growth rates below the long-term mean for seven years in a row. After a prolonged hunger period the fish stock collapsed in 1992 also by the impact of fisheries - and this was probably not a coincidence. Now, with the reference-growth rate GRF a simple and handy parameter was found to summarize the influence of the environmental conditions on growth and other derived models and therefore makes it easier to compute the influence of environmental changes within stock assessment. Zusammenfassung Veränderungen der Umwelt können Auswirkungen auf die Lebensbedingungen der Fische haben, z. B. auf das Nahrungsangebot der Fische. Die vorherrschenden Umgebungsbedingungen wirken gleichmäßig auf alle Altersgruppen eines Bestandes, wobei typischer Weise kleineFische hohe Wachstumsraten haben, während die großen Fische mit niedrigen Raten wachsen. Auf der Grundlage des von Bertalanffy-Wachstumsmodells kann gezeigt werden, dass es ausreicht, nur die Wachstumsrate von einer einzigen Altersgruppe zu kennen, um die Wachstumsraten von allen anderen Altersgruppen berechnen zu können. Die Wachstumsrate eines Referenz-Fisches (z.B. eines Fisches mit einer Körpermasse von 1 kg) wurde als Referenz-Wachstum GRF eingeführt, die den aktuellen Zustand des Nahrungsangebots füralle Altersgruppen des Bestandes beschreibt. Als Beispiel wurde einer Zeitreihe der Referenz-Wachstumsraten des nördlichen Kabeljaubestandes (NAFO, 3K) für die Zeitsraum 1979 bis 1999 berechnet. Für diesen Kabeljaubestand war zu beobachten, dass Umgebungsbedingungen für sieben Jahre in Folge Wachstumsraten unter dem langjährigen Mittelwert verursachten. Nach einer längeren Hungerperiode kollabierte dieser Fischbestand im Jahr 1992 auch durch den Einfluß der Fischerei - und dies war sicher kein Zufall. Jetzt, mit der Referenz-Wachstumsrate GRF, ist ein einfacher und handlicher Parameter gefunden, der es gestattet den Einfluss der Umweltbedingungen auf die Wachstumsbedingungen und andere davon abgeleitete Modelle zusammenzufassen. Dies macht es einfach, den Einfluss von Umweltveränderungen innerhalb der Bestandsabschätzungen zu berechnen.

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The zooplankton and macrobenthic communities of Lake Victoria were sampled by lift net and Ponar grab, respectively. The zooplankton comprised copepods and cladocerans, rotifers and aquatic insect larvae. Most taxa exhibited wide distribution in the lake, with the exception of rotifers which were rare in deep offshore waters. The main components in the macro-benthos were chaoborid and chironomid larvae and molluscs. Caridina nilotica (Roux) and other groups were rare in the samples. Zooplankton density ranged from 100000 or more to 4 million ind. m2 and increased from the shallow inshore to deep offshore waters. Numerical dominance of cyclopoids and nauplius larvae was a common feature at all stations sampled. Most macrobenthic taxa were also widely distributed, although chaoborid and chironomid larvae were rare in the samples. Rastrineobola argentea (Pellegrin) and larval Lates niloticus (L.) ate mainly cyclopoid copepods, while cichlids showed a strong preference for adult insects. High ecological stability of the cyclopoids, and the zooplankton community in general, despite radical ecosystem changes in recent years, coupled with what appears to be high predation pressure, offers good prospects for the pelagic fishery in the lake.

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Caridina nilotica (Roux) (Decapoda: Atyidae) was investigated over a period of four months in three zones of Lake Victoria. Abundances were estimated by vertical net sampling. The importance of C. nilotica in the diet of the three commercial fish species was investigated. Caridina nilotica is a primary food for Lates niloticus (L.), Oreochromis niloticus (L.) and Rastrineobola argentea (Pellegrin). A quantitative study of C. nilotica in the fishing area indicated high biomass which can support the Lake Victoria fisheries.

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Researchers compared nest architecture in loggerhead sea turtles at natural beaches in Florida, USA and Brazil to determine how similarities and differences in female morphology and reproductive output in these two populations are reflected in the structure of the nest.

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This presentation will describe the use of online forums in Moodle, a course management system, to teach students to evaluate both print and electronic reference sources. For two semesters I have created an online forum called the Fishing Hole Scouting Report for an in-class exercise. Students are given 2-3 background questions and told to find the answers using both a library-approved reference source and Wikipedia. Students then evaluate the reference source for effectiveness and ease of use, compared to Wikipedia, and post their comments in the forum to share with the class. I will highlight the educational benefits of using Moodle forums for this purpose and discuss best practices for selecting the most effective sources and questions.

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Thermodynamical fluctuations in temperature and position exist in every physical system, and show up as a fundamental noise limit whenever we choose to measure some quantity in a laboratory environment. Thermodynamical fluctuations in the position of the atoms in the dielectric coatings on the mirrors for optical cavities at the forefront of precision metrology (e.g., LIGO, the cavities which probe atomic transitions to define the second) are a current limiting noise source for these experiments, and anything which involves locking a laser to an optical cavity. These thermodynamic noise sources scale physical geometry of experiment, material properties (such as mechanical loss in our dielectric coatings), and temperature. The temperature scaling provides a natural motivation to move to lower temperatures, with a potential huge benefit for redesigning a room temperature experiment which is limited by thermal noise for cryogenic operation.

We design, build, and characterize a pair of linear Fabry-Perot cavities to explore limitations to ultra low noise laser stabilization experiments at cryogenic temperatures. We use silicon as the primary material for the cavity and mirrors, due to a zero crossing in its linear coefficient of thermal expansion (CTE) at 123 K, and other desirable material properties. We use silica tantala coatings, which are currently the best for making high finesse low noise cavities at room temperature. The material properties of these coating materials (which set the thermal noise levels) are relatively unknown at cryogenic temperatures, which motivates us to study them at these temperatures. We were not able to measure any thermal noise source with our experiment due to excess noise. In this work we analyze the design and performance of the cavities, and recommend a design shift from mid length cavities to short cavities in order to facilitate a direct measurement of cryogenic coating noise.

In addition, we measure the cavities (frequency dependent) photo-thermal response. This can help characterize thermooptic noise in the coatings, which is poorly understood at cryogenic temperatures. We also explore the feasibility of using the cavity to do macroscopic quantum optomechanics such as ground state cooling.

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The centralized paradigm of a single controller and a single plant upon which modern control theory is built is no longer applicable to modern cyber-physical systems of interest, such as the power-grid, software defined networks or automated highways systems, as these are all large-scale and spatially distributed. Both the scale and the distributed nature of these systems has motivated the decentralization of control schemes into local sub-controllers that measure, exchange and act on locally available subsets of the globally available system information. This decentralization of control logic leads to different decision makers acting on asymmetric information sets, introduces the need for coordination between them, and perhaps not surprisingly makes the resulting optimal control problem much harder to solve. In fact, shortly after such questions were posed, it was realized that seemingly simple decentralized optimal control problems are computationally intractable to solve, with the Wistenhausen counterexample being a famous instance of this phenomenon. Spurred on by this perhaps discouraging result, a concerted 40 year effort to identify tractable classes of distributed optimal control problems culminated in the notion of quadratic invariance, which loosely states that if sub-controllers can exchange information with each other at least as quickly as the effect of their control actions propagates through the plant, then the resulting distributed optimal control problem admits a convex formulation.

The identification of quadratic invariance as an appropriate means of "convexifying" distributed optimal control problems led to a renewed enthusiasm in the controller synthesis community, resulting in a rich set of results over the past decade. The contributions of this thesis can be seen as being a part of this broader family of results, with a particular focus on closing the gap between theory and practice by relaxing or removing assumptions made in the traditional distributed optimal control framework. Our contributions are to the foundational theory of distributed optimal control, and fall under three broad categories, namely controller synthesis, architecture design and system identification.

We begin by providing two novel controller synthesis algorithms. The first is a solution to the distributed H-infinity optimal control problem subject to delay constraints, and provides the only known exact characterization of delay-constrained distributed controllers satisfying an H-infinity norm bound. The second is an explicit dynamic programming solution to a two player LQR state-feedback problem with varying delays. Accommodating varying delays represents an important first step in combining distributed optimal control theory with the area of Networked Control Systems that considers lossy channels in the feedback loop. Our next set of results are concerned with controller architecture design. When designing controllers for large-scale systems, the architectural aspects of the controller such as the placement of actuators, sensors, and the communication links between them can no longer be taken as given -- indeed the task of designing this architecture is now as important as the design of the control laws themselves. To address this task, we formulate the Regularization for Design (RFD) framework, which is a unifying computationally tractable approach, based on the model matching framework and atomic norm regularization, for the simultaneous co-design of a structured optimal controller and the architecture needed to implement it. Our final result is a contribution to distributed system identification. Traditional system identification techniques such as subspace identification are not computationally scalable, and destroy rather than leverage any a priori information about the system's interconnection structure. We argue that in the context of system identification, an essential building block of any scalable algorithm is the ability to estimate local dynamics within a large interconnected system. To that end we propose a promising heuristic for identifying the dynamics of a subsystem that is still connected to a large system. We exploit the fact that the transfer function of the local dynamics is low-order, but full-rank, while the transfer function of the global dynamics is high-order, but low-rank, to formulate this separation task as a nuclear norm minimization problem. Finally, we conclude with a brief discussion of future research directions, with a particular emphasis on how to incorporate the results of this thesis, and those of optimal control theory in general, into a broader theory of dynamics, control and optimization in layered architectures.