ORLANDO COSTAS: ENSAIOS NO CAMINHO PARA UMA PASTORAL EVANGÉLICA LATINO-AMERICANA

Nesta pesquisa se propõe analisar a pastoral evangélica latino-americana elaborada por Orlando Costas. Teólogo pastoralista, missiólogo e de origem porto-riquenha, Costas elaborou método e metodologia de pastoral a partir de sua confissão religiosa evangelical em diálogo ecumênico e em consonância crítica com a situação política, econômica, social e religiosa de Porto Rico e da América Latina. Costas, juntamente com Emilio Castro, foi um dos primeiros a lançar as bases para uma pastoral evangélica visando ao homem latino-americano. Criticou o modelo da teologia pastoral norte-atlântica centrada no pastor com sujeito da ação pastoral da igreja alegando ser; repetitiva, profissional e eclesiocêntrica. A pesquisa foi realizada em três passos, que se realizam nos capítulos um, dois e três, respectivamente, compreender o contexto político, econômico, social e religioso da vida de Orlando Costas a partir de Porto Rico, suas experiências de conversão-ruptura dentro da sua tradição religiosa e a nova percepção pastoral; analisar o desenvolvimento histórico teológico da pastoral evangélica, ou seja, os movimentos, conferências, instituições e teólogos que influenciaram o pensamento de Costas; e, apresentar os fundamentos missiológicos e teológicos da pastoral evangélica, em que se demonstra que a pastoral de Costas é missiológico-pastoral, pois assume a missio Dei como princípio arquitetônico e a pastoral como princípio hermenêutico para a ação missional e pastoral. Conclui-se que a proposta de Costas é de uma pastoral evangélica e ecumênica, contextual e autóctone, dentro e fora da igreja.(AU)

Avaliação dos efeitos tóxicos in vitro e in vivo do extrato hidroetanólico dos frutos de Genipa americana L. (Rubiaceae) em camundongos Swiss

The therapeutic use of medicinal plants has contributed since antiquity in a beneficial way for health. However, many species lacks of scientific evidence which provide basis for their use in therapeutic practice. In this context is the Genipa americana L. species (Rubiaceae), popularly known as jenipapo and used to treat syfilis, ulcer and hemorrhagic disturbs. It's also used against bruising, as tonic and as aphrodisiac. Due this species lacks toxicological studies, the aim of this study was to evaluate the toxicity in vivo (acute and sub-chronic toxicity) and in vitro (cytotoxicity) of the hydroethanolic extract from G. americana fruits. The hydroethanolic extract of G. americana fruits was prepared by maceration. A preliminary phytochemical analysis was performed to assess the presence of secondary metabolites in the extract. The cytotoxicity study of the extract (0.1, 1.0, 10, 100 and 1000 mg / 100 ul) were performed against normal cells (3T3) and tumor (786-0, HepG2 and B16), analyzed by the MTT assay. To evaluate the acute (single dose of 2000 mg / Kg) and subchronic (100, 500 and 1000 mg / kg for 30 days) toxicity Swiss mice of both sexes were used. At the end of the experiment, blood samples and organs were collected for analysis. Data between groups were compared by t test or ANOVA with Dunnett's post-test with 5% significance level. The phytochemical study of the extracts mainly indicated the presence of iridoids. Results for cytotoxicity tests showed up to 70% inhibition of B16 cell line at a dose of 1000 mg / 100 ul, and up to 29% inhibition of 786-0 at a dose of 10 ug / 100 ul. The extract did not cause death in 3T3 and HepG2 cells. During the in vivo assays, there were no animal deaths. Analysis of blood samples revealed that the animals submitted to the evaluation of acute toxicity had changes in AST and ALT, and that the animals evaluated for subchronic toxicity showed changes in the relative wet weight of the kidney and plasma urea concentration. No differences were observed between groups on histopathological evaluation of the collected organs. Despite the changes found in the in vivo toxicity tests, using the criteria described by the OECD Guidelines, it is suggested that the hydroethanolic extract of the fruits of the G. americana is classified as low toxicity. The cytotoxicity of the extract suggests that they have potential against melanoma cell lines (B16).

Avaliação dos efeitos tóxicos in vitro e in vivo do extrato hidroetanólico dos frutos de Genipa americana L. (Rubiaceae) em camundongos Swiss

The therapeutic use of medicinal plants has contributed since antiquity in a beneficial way for health. However, many species lacks of scientific evidence which provide basis for their use in therapeutic practice. In this context is the Genipa americana L. species (Rubiaceae), popularly known as jenipapo and used to treat syfilis, ulcer and hemorrhagic disturbs. It's also used against bruising, as tonic and as aphrodisiac. Due this species lacks toxicological studies, the aim of this study was to evaluate the toxicity in vivo (acute and sub-chronic toxicity) and in vitro (cytotoxicity) of the hydroethanolic extract from G. americana fruits. The hydroethanolic extract of G. americana fruits was prepared by maceration. A preliminary phytochemical analysis was performed to assess the presence of secondary metabolites in the extract. The cytotoxicity study of the extract (0.1, 1.0, 10, 100 and 1000 mg / 100 ul) were performed against normal cells (3T3) and tumor (786-0, HepG2 and B16), analyzed by the MTT assay. To evaluate the acute (single dose of 2000 mg / Kg) and subchronic (100, 500 and 1000 mg / kg for 30 days) toxicity Swiss mice of both sexes were used. At the end of the experiment, blood samples and organs were collected for analysis. Data between groups were compared by t test or ANOVA with Dunnett's post-test with 5% significance level. The phytochemical study of the extracts mainly indicated the presence of iridoids. Results for cytotoxicity tests showed up to 70% inhibition of B16 cell line at a dose of 1000 mg / 100 ul, and up to 29% inhibition of 786-0 at a dose of 10 ug / 100 ul. The extract did not cause death in 3T3 and HepG2 cells. During the in vivo assays, there were no animal deaths. Analysis of blood samples revealed that the animals submitted to the evaluation of acute toxicity had changes in AST and ALT, and that the animals evaluated for subchronic toxicity showed changes in the relative wet weight of the kidney and plasma urea concentration. No differences were observed between groups on histopathological evaluation of the collected organs. Despite the changes found in the in vivo toxicity tests, using the criteria described by the OECD Guidelines, it is suggested that the hydroethanolic extract of the fruits of the G. americana is classified as low toxicity. The cytotoxicity of the extract suggests that they have potential against melanoma cell lines (B16).

El arte camaleónico de Marcos Irizarry: entre el modernismo europeo y la abstracción americana, (1950-1970)

Marcos Irizarry (Mayagüez, Puerto Rico 1936-San Juan, Puerto Rico 1995) es uno de los artistas plásticos puertorriqueños más destacados e influyentes que creó una de las obras gráficas y pictóricas abstractas más significativas en Puerto Rico y España. La presente investigación tiene como objetivo el estudio y análisis de la obra de Marcos Irizarry. El estudio de la obra de Marcos Irizarry es importante, ya que los trabajos y escritos realizados tanto en Puerto Rico como en España son limitados, y mayormente se basan en el contexto puertorriqueño. El objetivo de esta investigación es contribuir a la historia del arte de Puerto Rico, de modo que se fomente el estudio, y se logre un mayor entendimiento de la abstracción y de la extensa obra gráfica del artista puertorriqueño Marcos Irizarry, sobre todo el que se dio durante las décadas sugeridas y mostrar su trascendencia, su gran legado, su amplio alcance y reafirmar su carácter universal. La hipótesis fundamental de este trabajo se basa en que la obra del artista puertorriqueño, Marcos Irizarry posee características, estéticas, conceptos y rasgos que pueden responder a las estipuladas tanto por el informalismo europeo-español o por la abstracción americana-puertorriqueña. Se concluyó que la obra gráfica realizada entre finales de los cincuenta hasta finales de los setenta, cobra carácter distinto si se ve como parte del proceso estético español de la posguerra o si se la sitúa en el ámbito del arte puertorriqueño...

Cryopreservation of avocado (Persea Americana Mill.) using somatic embryos

Avocado genetic resources are currently maintained in the form of field repositories at great cost and risk of natural disasters, pest and diseases. Cryopreservation offers a necessary, complimentary method that is safe, cost-effective and long-term. However, long-term maintenance and regeneration of plantlets from avocado somatic embryos has been a major barrier in the development of new avocado cultivars. In this study, two protocols for vitrification-based cryopreservation of avocado somatic embryos were investigated. Globular somatic embryos of two avocado cultivars were tested, revealing cultivar-dependent differences in desiccation tolerance and subsequent freezing resistance, possibly attributed to their size and culture age. A two-step regeneration system, involving an intermediate liquid phase step between subcultures in solid medium, significantly enhanced shoot development from somatic embryo tissue. This work will add considerable value towards cryopreservation of avocado somatic embryos for germplasm conservation and the generation of new and improved avocado cultivars.

Americanismo e fordismo nos boletins da comissão brasileiro-americana de educação industrial

This text thematizes the performance of the Brazilian-American Commission of Industrial Education (CBAI) since its installation at Rio de Janeiro, on 1947, and extinction in Curitiba, on 1963. The general goal consists in identifying if are there any relation between Gramsci’s Americanism and Fordism elements and the CBAI’s performance, by means of a speech analysis from de Newsletter of CBAI and other documental sources related to the organizations performance. The specifics objectives intend to contextualize the political and economic situation that Brazil was going through before and concomitant to CBAI’s performance, emphasizing some aspects of the Cold War feature that contributed to narrow the relations between United States and other countries of Latin America, especially Brazil. On the following, it intends to present the main aspects of Gramsci’s thought and the Americanism and fordism and Passive Revolution as key categories for a better understanding of the presence of an Americanization project on Brazilian’s professional education. As so, the object of this study are the Newsletters of CBAI. Finally, the speech’s analisys of the Newsletter was the methodology used to demonstrate CBAI as an Americanism diffuser. The documental research and sources served as groundwork, especially the Newsletters, were found at Departamento de Documentação Histórica of Universidade Tecnológica Federal do Paraná (DEDHIS) and at Biblioteca de Educação of Universidade de São Paulo (FEUSP). The theoretical foundation has as a workline for the conception of the categories the studies of Gramsci about the of work (and the Newsletters itself), and the speech’s analysis of main concepts from Bakhtin, Voloshinov’s and the Circle of studies about language philosophy. At last, this paperwork concludes that the attempt to disseminate an amerizanization project in Brazil obtneined significant results on the industrialization of the country according to the fordism’s racionalization standarts, nevertheless, this research considers that such a project corroborates the comprehension about the consolidation of a Passive Revolution’s project.

Aspectos sanitários do cultivo da alface americana, irrigada com águas recptoras de efluentes urbanos

Avaliou-se a influência da disposição de mangueiras gotejadoras nos canteiros e a injeção ou não de cloro na água de irrigação, nas condições sanitárias do solo e da alface americana irrigada (Lactuca sativa L.) com águas receptoras de efluentes urbanos. Foram realizadas análises microbiológicas de amostras de água do solo e da alface, no decorrer de todo o ciclo de cultivo. Objetivou-se determinar a possível existência de Salmonella spp. e de formas evolutivas de parasitos humanos e a quantidade de coliformes fecais, em pontos e épocas diferentes do experimento, impedindo assim o consumo da alface. Os resultados não indicaram a presença dos dois primeiros em nenhuma das amostras, mas sim de parasitos não humanos (nematóides) de vida livre no solo. em relação à quantidade de coliformes fecais (NMP ml-1), o valor encontrado na cultura atende às exigências da Secretaria de Vigilância Sanitária do Ministério da Saúde brasileiro, porém a presença dos nematóides em quantidades superiores ao permitido pela Organização Mundial de Saúde (OMS) inviabiliza o seu consumo.

Chemotaxonomic studies on Schwenckia americana LINN.

This study investigated the chemotaxonomic studies on Schwenckia americana Linn., a member of the family Solanaceae predominantly found mostly in low grass fields, Nigeria. The habit is annual herbaceous weed with slender stem characterized with free branching and growing up to 45cm in height.They are used mainly as medicine. The leaves are simple, entire, elliptic to ovate in shape, smooth, variable, petiolate and larger at the lower region of stem and narrowing to smaller almost sessile and oblanceolate towards the apical regions which are alternately arranged and acrescently structured from the top to the base upto 3.7 ± 1.5cm long and 2.4±0.6cm wide. The inflorescence is a panicle of 15 or more flowers occurring at stem terminal.The flowers are whitish tubular structures measuring up to 1.0±0.2cm in length and 0.1cm in diameter. The petals are whitish up to 0.9 ±0.2cm in length and sepals are greenish up to 0.1cm in length. The berry fruit is greenish when unripe and pale yellowish when ripe up to 0.3±0.1cm in diameter. The seeds are blackish and spherical or triangular shaped with rough edges measuring up to 0.1cm in diameter. The epidermal studies reveal anomocytic stomata whereas the trichomes are simple uniseriate forms wth bulge heads. The anatomy of mid-ribs and petioles showed bicollateral vascular systems. There are three vascular traces and the node is unilacunar. The petioles have 2 rib traces at primary growth phase. At secondary growth phase, the mid-rib and petiole revealed vascular arcs and the stem has a ring of open vascular system. The cytological studies showed a diploid chromosome number of 2n = 24 and n = 12 for the haploids. Alkaloids, saponins, tannins, phlobatannins, flavonoids, combined anthraquinones, free anthraquinones and cardiac glycosides are present.

UV-Vis, FTIR and antioxidant study of Persea Americana (Avocado) leaf and fruit: A comparison

El aguacate (Persea americana Miller) es una conocida fruta arb´orea con un alto contenido nutricional que crece en varias partes del mundo. El presente estudio compara los espectros del UV-Vis y del espectr´ometro infrarrojo con transformada de Fourier (FTIR) de la fruta y de la hoja de aguacate (c´ascara, pulpa y aceite) cultivado en Ecuador y posteriormente eval´ua su actividad antioxidante empleando el 1,1-difenil-2-picrilhidrazil (DPPH•). El estudio de los espctros UV-Vis y FTIR revel´o que el aguacate tiene predominantemente flavonoides. Entre la hoja y el fruto del aguacate, se comprob´o mediante el ensayo DPPH• (captura de radicales libres), que la hoja tuvo una mayor actividad antioxidante, la que oscila entre 84,46% y 80,12%, con valores de 32.60-32.73 μg equivalentes de ´acido g´alico por mL. Se demostr´o que el orden de la actividad antioxidante de los extractos es: hoja de aguacate > c´ascara > aceite > pulpa. La actividad antioxidante tuvo una correlaci´on positiva con el contenido total de flavonoides y estos extractos de plantas (especialmente de las hojas del aguacate) son ´utiles para el desarrollo de futuros productos antioxidantes.