The E(NK) model: extending the NK model to incorporate gene-by-environment interactions and epistasis for diploid genomes

The haploid NK model developed by Kauffman can be extended to diploid genomes and to incorporate gene-by-environment interaction effects in combination with epistasis. To provide the flexibility to include a wide range of forms of gene-by-environment interactions, a target population of environment types (TPE) is defined. The TPE consists of a set of E different environment types, each with their own frequency of occurrence. Each environment type conditions a different NK gene network structure or series of gene effects for a given network structure, providing the framework for defining gene-by-environment interactions. Thus, different NK models can be partially or completely nested within the E environment types of a TPE, giving rise to the E(NK) model for a biological system. With this model it is possible to examine how populations of genotypes evolve in context with properties of the environment that influence the contributions of genes to the fitness values of genotypes. We are using the E(NK) model to investigate how both epistasis and gene-by-environment interactions influence the genetic improvement of quantitative traits by plant breeding strategies applied to agricultural systems. © 2002 Wiley Periodicals, Inc.

Play Preferences and Behavior of Preschool Children with Autistic Spectrum Disorder in the Clinical Environment

The play of children with autistic spectrum disorder (ASD) is a valuable medium for assessment and intervention, and its analysis has the potential to aid diagnosis. This study investigated spontaneous play behavior and play object preferences for 24 preschool children with ASD in a typical occupational therapy clinical environment. Play behavior was rated and choice of play object noted at 10-second intervals from a 15-minute video recording of unstructured play. Statistical analyses indicated that play behavior was consistent with descriptions in the literature. In addition, the children demonstrated clear preferences for play objects in the form of popular characters (e.g., Thomas the Tank Engine) and those with sensorimotor properties. We propose that the inclusion of preferred play objects in a clinical environment may increase intrinsic motivation to play, and thereby enhance assessment and intervention.

The mouse secretome: functional classification of the proteins secreted into the extracellular environment

We have developed a computational strategy to identify the set of soluble proteins secreted into the extracellular environment of a cell. Within the protein sequences predominantly derived from the RIKEN representative transcript and protein set, we identified 2033 unique soluble proteins that are potentially secreted from the cell. These proteins contain a signal peptide required for entry into the secretory pathway and lack any transmembrane domains or intracellular localization signals. This class of proteins, which we have termed the mouse secretome, included >500 novel proteins and 92 proteins

Transposition of first digital pad for reconstruction of a palmar antebrachial soft tissue defect in a cat

An 8-year-old female neutered Siamese cat was presented with a recent history of incomplete excision of an apocrine gland adenocarcinoma from the palmar aspect of the right antebrachium, just proximal to the carpal joint. There was no evidence of metastasis. Wide surgical excision of the previous surgery site was performed resulting in a soft tissue defect. Partial reconstruction was achieved using digital pad transposition of the first digit (dewclaw), forming a local axial pattern flap that was transposed into the adjacent defect. The remaining defect was closed by primary apposition. The skin flap healed successfully. Some breakdown of the skin closed by primary apposition necessitated open wound management. The cosmetic and functional result of the first digital pad transposition was considered excellent, rendering it a useful means to reconstruct soft tissue defects in the carpal region.

Characterization of Treponema phagedenis-like spirochetes isolated from papillomatous digital dermatitis lesions in dairy cattle

Four spirochete strains were isolated from papillomatous digital dermatitis (PDD) lesions in Iowa dairy cattle and compared with two previously described spirochete strains isolated from dairy cattle in California. These six strains shared an identical 16S ribosomal DNA sequence that was 98% similar to Treponema phagedenis and 99% similar to the uncultivated PDD spirochete sequence DDLK-4. The whole-cell protein profiles resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of these six strains were similar. However, these strains showed differences in the antigenic diversity of lipopolysaccharide (LPS). Genetic diversity was also detected by pulsed-field gel electrophoresis of genomic DNA digests, revealing differences among five of the six strains. Serum immunoglobulin G antibodies from dairy cattle with active PDD lesions reacted with the LPS of all but one PDD spirochete strain. Likewise, peripheral blood mononuclear cells from cattle with active PDD lesions produced blastogenic responses to one of the two California isolates. Both antibody and lymphocyte blastogenic responses were reduced in convalescent dairy cattle, suggesting the immune response to these spirochetes has short duration. These results demonstrate genetic and antigenic diversity among T. phagedenis-like treponemes and provide further evidence for the involvement of these spirochetes in the pathogenesis of PDD.

Genotype and environment effects on dynamics of harvest index during grain filling in sorghum

An approach based on a linear rate of increase in harvest index (141) with time after anthesis has been used as a simple means-to predict grain growth and yield in many crop simulation models. When applied to diverse situations, however, this approach has been found to introduce significant error in grain yield predictions. Accordingly, this study was undertaken to examine the stability of the HI approach for yield prediction in sorghum [Sorghum bicolor (L.) Moench]. Four field experiments were conducted under nonlimiting water. and N conditions. The experiments were sown at times that ensured a broad range in temperature and radiation conditions. Treatments consisted of two population densities and three genotypes varying in maturity. Frequent sequential harvests were used to monitor crop growth, yield, and the dynamics of 111. Experiments varied greatly in yield and final HI. There was also a tendency for lower HI with later maturity. Harvest index dynamics also varied among experiments and, to a lesser extent, among treatments within experiments. The variation was associated mostly with the linear rate of increase in HI and timing of cessation of that increase. The average rate of HI increase was 0.0198 d(-1), but this was reduced considerably (0.0147) in one experiment that matured in cool conditions. The variations found in IN dynamics could be largely explained by differences in assimilation during grain filling and remobilization of preanthesis assimilate. We concluded that this level of variation in HI dynamics limited the general applicability of the HI approach in yield prediction and suggested a potential alternative for testing.

Evaluating plant breeding strategies by simulating gene action and dryland environment effects

Functional genomics is the systematic study of genome-wide effects of gene expression on organism growth and development with the ultimate aim of understanding how networks of genes influence traits. Here, we use a dynamic biophysical cropping systems model (APSIM-Sorg) to generate a state space of genotype performance based on 15 genes controlling four adaptive traits and then search this spice using a quantitative genetics model of a plant breeding program (QU-GENE) to simulate recurrent selection. Complex epistatic and gene X environment effects were generated for yield even though gene action at the trait level had been defined as simple additive effects. Given alternative breeding strategies that restricted either the cultivar maturity type or the drought environment type, the positive (+) alleles for 15 genes associated with the four adaptive traits were accumulated at different rates over cycles of selection. While early maturing genotypes were favored in the Severe-Terminal drought environment type, late genotypes were favored in the Mild-Terminal and Midseason drought environment types. In the Severe-Terminal environment, there was an interaction of the stay-green (SG) trait with other traits: Selection for + alleles of the SG genes was delayed until + alleles for genes associated with the transpiration efficiency and osmotic adjustment traits had been fixed. Given limitations in our current understanding of trait interaction and genetic control, the results are not conclusive. However, they demonstrate how the per se complexity of gene X gene X environment interactions will challenge the application of genomics and marker-assisted selection in crop improvement for dryland adaptation.

e-Processo: Processo Administrativo Digital

A iniciativa visa ?? extin????o do uso de papel nos processos dentro do ??rg??o p??blico mediante a cria????o do Processo Administrativo Digital (e-Processo). A promo????o da transpar??ncia dos atos p??blicos, com gest??o do conhecimento, maior autonomia e descentraliza????o administrativa, fortalece a democracia,melhora o ambiente de neg??cio e a confian??a no governo. O sistema foi implantado em todas as ??reas de neg??cios de 700 unidades administrativas, com 33 mil servidores conectados nos tr??s ??rg??os p??blicos federais: Receita Federal do Brasil (RFB), Procuradoria da Fazenda Nacional (PFN) e Conselho Administrativo de Recursos Fiscais (Carf). A substitui????o de um documento papel por um documento imagem tem o cond??o de promover no Estado uma verdadeira reengenharia procedimental e administrativa

Comunicação interna em instituições públicas de educação : avaliação do informativo digital do Instituto Federal do Espírito Santo-IFES

O presente trabalho visa estudar a ferramenta de comunicação interna de uma instituição pública de educação- um informativo digital, por meio da avaliação dos atributos necessários para que uma notícia seja divulgada neste veículo. Dessa maneira, se busca criar um documento com um elenco de valores-notícia que norteiem a seleção, produção e divulgação de notícias no informativo digital e, assim, contribuir para a melhoria da comunicação interna na organização. A avaliação das matérias e a criação deste documento utilizarão como referencial os valores-notícia já utilizados no jornalismo para definir o que é notícia.

Hibridizando o ensino de inglês na Escola de Aprendizes- Marinheiros do Espírito Santo

Esta pesquisa teve por objetivo avaliar o desempenho em inglês como L2 de alunos submetidos a atividades em uma abordagem híbrida com o uso de ferramentas da internet na Escola de Aprendizes-Marinheiros do Espírito Santo. Quarenta alunos de duas turmas intactas foram divididos em dois grupos, o experimental (ensino tradicional + ambiente virtual) e o controle (ensino tradicional apenas). O referencial teórico revisou a literatura sobre a abordagem de ensino de línguas estrangeiras baseada em tarefas, metodologias híbridas de ensino de L2, multiletramentos e novas tecnologias. A metodologia de pesquisa usada foi mista com dados qualitativos e quantitativos. O estudo avaliou o impacto de atividades on-line na aprendizagem de L2 e no desenvolvimento da autonomia e do letramento digital. Três tarefas usando variados sítios da internet foram administradas ao grupo experimental, seguidas de um questionário para cada uma, usado para a avaliação qualitativa. A análise quantitativa foi feita por meio de pré e pós-testes analisados estatisticamente. Para esta pesquisa-ação, foram utilizados questionários, entrevistas semiestruturadas e o diário da professora-pesquisadora como instrumentos de coleta de dados. De forma geral, os resultados não mostraram ganhos estatisticamente significativos no tratamento, porém a análise qualitativa das impressões dos alunos-participantes sobre as tarefas realizadas revelou que a abordagem híbrida de ensino de L2 pode ajudar os alunos a desenvolver a autonomia, motivação, um maior contato com a língua-alvo e o letramento digital.