Fluorescence characterization and gain studies on a dye-doped graded index polymer optical-fiber preform

Preparation of an appropriate optical-fiber preform is vital for the fabrication of graded-index polymer optical fibers (GIPOF), which are considered to be a good choice for providing inexpensive high bandwidth data links, for local area networks and telecommunication applications. Recent development of the interfacial gel polymerization technique has caused a dramatic reduction in the total attenuation in GIPOF, and this is one of the potential methods to prepare fiber preforms for the fabrication of dye-doped polymer-fiber amplifiers. In this paper, the preparation of a dye-doped graded-index poly(methyl methacrylate) (PMMA) rod by the interfacial gel polymerization method using a PMMA tube is reported. An organic compound of high-refractive index, viz., diphenyl phthalate (DPP), was used to obtain a graded-index distribution, and Rhodamine B (Rh B), was used to dope the PMMA rod. The refractive index profile of the rod was measured using an interferometric technique and the index exponent was estimated. The single pass gain of the rod was measured at a pump wavelength of 532 nm. The extent of doping of the Rh B in the preform was studied by axially exciting a thin slice of the rod with white light and measuring the spatial variation of the fluorescence intensity across the sample.

Fabrication and Characterisation of Dye Doped Polymer Optical Fibres With Different Refractive Index Profiles for Photonic Applications

International School of Photonics, Cohin University of Science and Technology

Studies on Ribotyping, Integron Genes and Pathogenicity of Marine Vibrios

A rare horizontal gene transfer event could be traced. The movement of the SXT element among the Vibrionaceae could be followed. This element was first reported from Vibrio cholerae and in this study the same could be confirmed in Vibrio alginolyticus. Events such as these, which take place with respect to other virulence/virulence associated genes, may lead to the emergence of pathogenic strains from hitherto non-pathogens or may even give rise to new pathogens. The results generated in the course of this study paves way for further characterization and detailed study, especially with respect to those strains which showed gastric fluid accumulation in the in vivo suckling mouse assay. Antibiotic resistance pattern shown by a sample population of Vibrios can be used for deciding treatment options. There is enough scope for further research on these topics towards generating basic knowledge, which can be of immense significance in human and aquaculture health.

‘Systematics and pathogenicity of Vibrionaceae associated T ¢ S with the larvae of M. rosenbergii in hatchery

Even though Bergey '5 Manual has been recognized globally as the guide to bacterial systematics, it has to be emphasized that descriptions given to a large extent are based on studies made with temperate isolates This leads one to conclude that any attempt to identify the tropical isolates with identification keys and tables generated from this information may lead to erroneous conclusions. And there is every possibility of the existence of genotypic and phenotypic variants or even nev. species in this part ofthe aquatic ecosystem. Applications ofa polythetic scheme of classification based on the principles of Numerical Taxonomy opens up exciting avenues for bringing to light, this possibility which otherwise would have been masked by the unidirectional approach as in monothetic schemes. Another added advantage of clustering a ‘natural’ bacterial population by numerical taxonomy, is the ease by which genotypic characterization could be performed on the clusters by selecting a representative from each cluster This helps overcome the practical impossibility of analyzing all the isolates in a pani:'_lar cluster. The genotypic characteizarion would either be mole °/o G-'rC. DNA-D.\_-X hybridization, DNA-RNA hybridization or DNA fingerprinting. Considering the requirement creating a broad base in the understanding of the family Vibrionaceae associated with the larvae ofM rosenbergii, the present work was undertaken to channelize every new information generated for developing appropriate managerial measures to protect the larvae from vibriosis during the unusually prolonged larval phase.

Enzyme reactions as index of freshness of Fish and Shellfish

An attempt has been made in this study to screen some fish muscle enzymes to assess their potential worth in testing the degree of freshness of fish. A problem with routine enzyme activity determinations is the complexity of the method of enzyme assay. Hence, in the present study as far as possible simple assay techniques were adopted. Several species were screened to assess the possibility of employing this procedure on a large scale. It is hoped that findings of this study will lead to the development of meaningful criteria in testing the freshness of fish. This thesis has been divided into five chapters

Molecular Approaches For Characterization And Determination Of Pathogenicity Of Vibrios With Special Reference To Vibrio Harveyi From Penaeus Monodon Larval Production Systems

The present study focuses on vibrios especially Vibrio harveyi isolated from shrimp (P. monodon) larval production systems from both east and west coasts during times of mortality. A comprehensive approach has been made to work out their systematics through numerical taxonomy and group them based on RAPD profiling and to segregate the virulent from non- virulent isolates based on the presence of virulent genes as well as their phenotypic expression. The information gathered has helped to develop a simple scheme of identification based on phenotypic characters and segregate the virulent from non virulent strains of V. harveyi.

Variation in radio refractivity index associated with formation of low - pressure systems

Numerous low - pressure systems form in the Arabian Sea and Bay of Bengal. These low-pressure systems are highly useful in bringing the rainfall over the Indian sub continent. The developments of these systems are accompanied by the reduction in air temperature and an increase in atmospheric humidity. The radio refractivity, which is a function of the atmospheric pressure, temperature and humidity, also changes following the development of these systems. Variation of radio refractive index and its vertical gradient are analysed for many low pressure systems formed over the Arabian Sea and Bay of Bengal. It is found that the atmosphere becomes super refractive associated with the formation of these systems, caused by the increase in humidity and decrease in temperature. The maximum gradient is observed near the surface layers, especially in the lowest 1 km. Super refraction leads to increased radar detection range and extension of radio horizon

Systematics and pathogenicity of vibrionaceae associated with the larvae of macrobrachium rosenbergii in hatchery

The great potential for the culture of non-penaeid prawns, especially Macrobrachium rosenbergii in brackish and low saline areas of Indian coastal zone has not yet been fully exploited due to the non availability of healthy seed in adequate numbers and that too in the appropriate period. In spite of setting up several prawn hatcheries around the country to satiate the ever growing demands for the seed of the giant fresh water prawn, the supply still remains fear below the requirement mainly due to the mortality of the larvae at different stages of the larval cycle. In a larval rearing system of Macrobrachium rosenbergii, members of the family Vibrionaceae were found to be dominant flora and this was especially pronounced during the times of mortality However, to develop any sort of prophylactic and therapeutic measures, the pathogenic strains have to be segregated from the lot. This would never be possible unless they were clustered based on the principles of numerical taxonomy It is with these objectives and requirements that the present work involving phenotypic characterization of the isolates belonging to the family Vibrionaceae and working out the numerical taxonomy, determination of mole % G+C ratio, segregation of the pathogenic strains and screening antibiotics as therapeutics at times of emergency, was carried out.

Health, Education and Employment in a Forward-Backward Dichotomy Based on Standard of Living Index for the Tribes in Kerala

The Paper unfolds the paradox that exists in the tribal community with respect to the development indicators and hence tries to cull out the difference in the standard of living of the tribes in a dichotomous framework, forward and backward. Four variables have been considered for ascertaining the standard of living and socio-economic conditions of the tribes. The data for the study is obtained from a primary survey in the three tribal predominant districts of Wayanad, Idukki and Palakkad. Wayanad was selected for studying six tribal communities (Paniya, Adiya, Kuruma, Kurichya, Urali and Kattunaika), Idukki for two communities (Malayarayan and Muthuvan) and Palakkad for one community (Irula). 500 samples from 9 prominent tribal communities of Kerala have been collected according to multistage proportionate random sample framework. The analysis highlights the disproportionate nature of socio-economic indicators within the tribes in Kerala owing to the failure of governmental schemes and assistances meant for their empowerment. The socio-economic variables, such as education, health, and livelihood have been augmented with SLI based on correlation analysis gives interesting inference for policy options as high educated tribal communities are positively correlated with high SLI and livelihood. Further, each of the SLI variable is decomposed using Correlation and Correspondence analysis for understanding the relative standing of the nine tribal sub communities in the three dimensional framework of high, medium and low SLI levels. Tribes with good education and employment (Malayarayan, Kuruma and Kurichya) have a better living standard and hence they can generally be termed as forward tribes whereas those with a low or poor education, employment and living standard indicators (Paniya, Adiya, Urali, Kattunaika, Muthuvans and Irula) are categorized as backward tribes

Application of the european customer satisfaction index to postal services. Structural equation models versus partial least squars

Customer satisfaction and retention are key issues for organizations in today’s competitive market place. As such, much research and revenue has been invested in developing accurate ways of assessing consumer satisfaction at both the macro (national) and micro (organizational) level, facilitating comparisons in performance both within and between industries. Since the instigation of the national customer satisfaction indices (CSI), partial least squares (PLS) has been used to estimate the CSI models in preference to structural equation models (SEM) because they do not rely on strict assumptions about the data. However, this choice was based upon some misconceptions about the use of SEM’s and does not take into consideration more recent advances in SEM, including estimation methods that are robust to non-normality and missing data. In this paper, both SEM and PLS approaches were compared by evaluating perceptions of the Isle of Man Post Office Products and Customer service using a CSI format. The new robust SEM procedures were found to be advantageous over PLS. Product quality was found to be the only driver of customer satisfaction, while image and satisfaction were the only predictors of loyalty, thus arguing for the specificity of postal services