Deciphering the synergism of endogenous glycoside hydrolase families 1 and 9 from Coptotermes gestroi

Termites can degrade up to 90% of the lignocellulose they ingest using a repertoire of endogenous and symbiotic degrading enzymes. Termites have been shown to secrete two main glycoside hydrolases, which are GH1 (EC 3.2.1.21) and GH9 (EC 3.2.1.4) members. However, the molecular mechanism for lignocellulose degradation by these enzymes remains poorly understood. The present study was conducted to understand the synergistic relationship between GH9 (CgEG1) and GH1 (CgBG1) from Coptotermes gestroi, which is considered the major urban pest of São Paulo State in Brazil. The goal of this work was to decipher the mode of operation of CgEG1 and CgBG1 through a comprehensive biochemical analysis and molecular docking studies. There was outstanding degree of synergy in degrading glucose polymers for the production of glucose as a result of the endo-β-1,4-glucosidase and exo-β-1,4-glucosidase degradation capability of CgEG1 in concert with the high catalytic performance of CgBG1, which rapidly converts the oligomers into glucose. Our data not only provide an increased comprehension regarding the synergistic mechanism of these two enzymes for cellulose saccharification but also give insight about the role of these two enzymes in termite biology, which can provide the foundation for the development of a number of important applied research topics, such as the control of termites as pests as well as the development of technologies for lignocellulose-to-bioproduct applications. © 2013 Elsevier Ltd.

Diversidade e sazonalidade da comunidade de ácaros em três clones de seringueira no sudeste do Estado da Bahia

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Florística e fitossociologia das espécies arbóreas de ocorrência e mata ciliar no Alto Rio Pardo, na estância climática de Caconde - SP

Pós-graduação em Ciências Biológicas (Biologia Vegetal) - IBRC

Síntesis: planificación y políticas públicas en 1982-1984 y perspectivas para la segunda mitad de la década = Summary: public planning and policies in 1982-1984 and prospects for the second half of the decade

Incluye Bibliografía

Istmo Centroamericano: la regulación de la distribución de energía eléctrica en los países con empresas privadas. Los casos de El Salvador, Guatemala, Nicaragua y Panamá

Incluye Bibliografía

Modernización y los cambios en las condiciones de vida de la mujer campesina = Modernization and the changing life chances of women in low-income rural families

Incluye Bibliografía

Uma análise geográfica das novas ruralidades e do controle social nas Vilas Rurais da Paz em Rolândia e João Inocente em Cambé

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Port throughputs in Latin America and the Caribbean in 2009 and the first half of 2010

This issue of the FAL Bulletin presents primary analytical data on port development in the region and analyses the impact of the economic crisis on port activity in Latin America in 2009. It also provides preliminary data on container port throughput through June 2010, which point to recovering activity at most of the region’s ports.

ECLAC Notes Nº 18

Regional Perspective Op-ed of ECLAC Executive Secretary, José Antonio Ocampo Over Half of Latin America's Children and Adolescents Are Poor Recommendations for Building Equity Achievements and Challenges in the Fight Against Poverty Social Expenditure in Latin America Rose 50% During the 1990s Inequity Holds Steady in the Region Families: Diagnosis and Public Policies Statistical appendix Recent titles and calendar of events

Carbono orgânico e polissacrídeos em agregados de um latossolo vermelho eutrófico em sequências de culturas sib a semedura direta

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Famílias de zona rural e urbana: características e concepções de adolescentes

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Stress, depressão e qualidade de vida em beneficiários de programas de transferência de renda

Pós-graduação em Psicologia do Desenvolvimento e Aprendizagem - FC

Uso de marcadores microssatélites para estimar parentescos dentro de progênies de polinização aberta de espécies arbóreas dióicas: um estudo de caso de Myracrodruon urundeuva (F.F. & M.F. Allemão)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

História da assistência social aos pobres em Londrina: 1940-1980

Pós-graduação em História - FCLAS