Alterações do fluxo sanguíneo em artéria umbilical na síndrome hipertensiva gestacional e suas implicações nos resultados neonatais

OBJETIVO: Avaliar as características antropométricas, a morbidade e mortalidade de recém-nascidos (RN) prematuros nascidos vivos de mães hipertensas em função da presença ou não de diástole zero (DZ) ou reversa (DR) na doplervelocimetria arterial umbilical. MÉTODOS: Estudo prospectivo, envolvendo RN prematuros nascidos vivos de gestantes hipertensas, com idade gestacional entre 25 e 33 semanas, submetidas à doplervelocimetria da artéria umbilical nos 5 dias que antecederam o parto, realizado no Hospital do Distrito Federal, entre 1º de novembro de 2009 e 31 de outubro de 2010. Os RN foram estratificados em dois grupos, conforme o resultado da doplervelocimetria da artéria umbilical: Gdz/dr=presença de diástole zero (DZ) ou diástole reversa (DR) e Gn=doplervelocimetria normal. Medidas antropométricas ao nascimento, morbidades e mortalidade neonatal foram comparadas entre os dois grupos. RESULTADOS: Foram incluídos 92 RN, assim distribuídos: Gdz/dr=52 RN e Gn=40 RN. No Gdz/dr a incidência de RN pequenos para idade gestacional foi significativamente maior, com risco relativo de 2,5 (IC95% 1,7‒3,7). No grupo Gdz/dr os RN permaneceram mais tempo em ventilação mecânica mediana 2 (0‒28) e no Gn mediana 0,5 (0‒25), p=0,03. A necessidade de oxigênio aos 28 dias de vida foi maior no Gdz/dr do que no Gn (33 versus10%; p=0,01). A mortalidade neonatal foi maior em Gdz/dr do que em Gn (36 versus 10%; p=0,03; com risco relativo de 1,6; IC95% 1,2 - 2,2). Nessa amostra a regressão logística mostrou que a cada 100 gramas a menos de peso ao nascer no Gdz/dr a chance de óbito aumentou 6,7 vezes (IC95% 2,0 - 11,3; p<0,01). CONCLUSÃO: em RN prematuros de mães hipertensas com alteração na doplervelocimetria da artéria umbilical a restrição do crescimento intrauterino é frequente e o prognóstico neonatal pior, sendo elevado o risco de óbito relacionado ao peso ao nascimento.

Diversidade genética, endogamia e fluxo gênico em pequena população fragmentada de Copaifera langsdorffii

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Metodologia e aplicação da citometria de fluxo na hematologia veterinária

A citometria de fluxo é um método emergente na medicina veterinária que permite a identificação e quantificação de células em suspensão. Apesar do custo elevado e da necessidade de técnicos especializados para realização da avaliação citofluorométrica, esta técnica tem uma ampla aplicação na hematologia veterinária, incluindo a avaliação de células-tronco hematopoiéticas, eritrócitos, leucócitos e plaquetas. O grande potencial de aplicação clínica da citometria de fluxo inclui a quantificação das células CD34+ como indicação da capacidade de reconstituição hematopoiética após o transplante de células-tronco e das subpopulações linfocitárias para avaliação da resposta imune frente aos transplantes e às doenças que acometem os animais. Projetos científicos sobre a avaliação citofluorométrica das células-tronco e subpopulações linfocitárias de cães têm sido desenvolvidos com sucesso por pesquisadores da área de hematologia veterinária na Universidade Estadual Paulista (UNESP), Campus de Jaboticabal. Portanto, no futuro, a citometria de fluxo deve se tornar uma técnica de rotina em laboratórios veterinários no Brasil.

Sucção de alta potência no controle das partículas emitidas pelo sistema de abrasão a ar em consultório odontológico

O objetivo do estudo foi avaliar a deposição de óxido de alumínio no campo operatório do cirurgião-dentista durante a utilização do sistema de abrasão a ar em consultório odontológico, bem como a efetividade da sucção de alta potência na captação desse pó. Por meio de um dispositivo para a coleta das partículas nos locais correspondentes às posições e distâncias de trabalho do profissional, dentes artificiais foram abrasionados. O sistema de sucção empregado para aspiração das partículas foi o de alta potência com sugador de saliva convencional e sugador com abertura ampliada por funil. A mensuração das partículas foi determinada pela quantidade em massa de óxido de alumínio depositada em placas de Petri. Os resultados obtidos por meio de estatística descritiva gráfica revelaram que a maior quantidade de pó se encontrava a 20 cm do operador e na posição de trabalho 9h, quando foi utilizado o sugador de saliva convencional. Uma vez comprovado que a sucção não é totalmente eficiente na aspiração do pó de óxido de alumínio, reforça-se a importância da proteção individual apropriada para o emprego seguro do sistema de abrasão a ar para pacientes e, principalmente, para os profissionais que trabalham com este tipo de tecnologia.

Sinterização de filmes finos de LiNbO3 em forno microondas: estudo da influência da direção do fluxo de calor

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Interações do ácido indolil-3-acético e calcio sobre o fluxo de água de células de Nitella

The effect of indol-3-acetic acid (lAA) and calcium (Ca + + ) on the flow of water through Nitella celIs was studied. Nitella cernua Braun was selected because it is possible tb study the permeability of a single isolated cell. IAA was used at a concentration of 0,28 10-3 M and CaCI2 at 10-3 M. The effect of IAA on the isolated celIs was confirmed by the increase of plasticity and permeability of the plasmalema of th.e treated cells with absorption and a consequent elimination of water. The effect was reversed by Ca ions.

Relação entre o fluxo luminoso interceptado em diferentes épocas no índice de área foliar de diferentes forrageiras

The objective was to evaluate the leaf area index of six different grasses. The experiment was installed at the Instituto Federal de Tecnologia e Educacao of Uberaba, using a randomized block design with split plots in time. The plots were sown: Brachiaria decumbens cv. Basilisk, Brachiaria brizantha cv. Marandu, Panicum maximum cv. Mombasa, Panicum maximum cv. Tanzania, Brachiaria brizantha cv. Xaraes, Cynodon dactylon hb. Tifton and the plots, ten seasons of evaluation in 10 x 6 factorial arrangement with four replications. Rates of leaf area of each forage at different times of year were evaluated. Compared to other forage species, Panicum maximum had a higher leaf area index when subjected to periodic fertilization and irrigation. Only in the late fall Brachiaria Xaraes IAF had increased, but did not differ significantly from the others.

Efeitos do pentobarbital sódico sobre o fluxo sanguíneo renal. Estudo experimental no cão

In six dogs, previously anesthetized with sodium pentobarbital (30 mg/kg) for surgical preparation, catheterism and monitoring, the action of sodium pentobarbital (7,5 mg/kg) on renal flow was studied. Determinations of mean arterial pressure, venous pressure, cardiac rate, arterio-cava pressure gradient and renal arterial resistance were made. Pentobarbital doesn't change significantly the renal blood flow or any of the other parameters studied, with the exception of venous pressure in the inferior caval vein where the drug produces a significant fall.

Efeito do extrato aquoso de sete-sangrias [Cuphea mesostemon (Koehne) Lourt] sobre o fluxo de água, medido em bexiga urinária de sapo in vitro

The Cuphea mesostemon specie, known as sete-sangrias, is widely used as a diuretic substance in popular medicine. As the toad urinary bladder is an epithelium analogous to the distal nephron of mammals, it is used in order to study the transport water and electrolytes in many laboratories. This preparation permits excellent observation in water flow, from the urinary bladder lumen to the external side or the serosal one (water re-absorption), by means of gravimetrical measures. In the present work the hydrosmotic effect of aqueous extract (AE) of sete-sangrias leaf was studied. A 20% solution was added to the serosal side (S) of the bladder preparation, and the water flow was measured every 15 minutes after that. The results showed that 4mL of AE in the S side, increased the JH20 in a significant manner (p<0,05). This effect had a dose - response shape, with the volumes of 0,2mL, 0,4mL and 0,8mL of AE in the S bath. The hydro-osmotic effect of the anti-diuretic hormone (ADH) was studied as well and a significant stimulation (p<0,05) in the JH2O was observed with the magnitude of 150%. The AE effect was similar to the ADH one, and was not antagonized by this hormone. We concluded that Cuphea possesses an anti-diuretic activity similar to that presented by ADH, in toad urinary bladder, in vitro.

Relação da potência aeróbica máxima e da força muscular com a economia de corrida em atletas de endurance

The objective of this study was to analyze the relationship of maximal aerobic power and the muscular strength (maximal isotonic strength and vertical jump explosive power) with the running economy (RE) in endurance athletes. Twenty-six male runners (27.9 ± 6.4 years; 62.7 ± 4.3 kg; 168.6 ± 6.1 cm; 6.6 ± 3.1% of body fat) performed in different days the following tests: a) incremental test to determine the maximal oxygen uptake (V̇O2max) and the intensity corresponding to the V̇O2max (IV̇O2max); b) constant-velocity treadmill run to determine RE; c) 1-RM test in the leg press and; d) maximal vertical jump test (VJ). V̇O2max (63.8 ± 8.3 ml/kg/min) was significantly correlated (r = 0.63; p < 0.05) with RE (48.0 ± 6.6 ml/kg/min). However, the IV̇O2max (18.7 ± 1.1 km/h), the maximal isotonic strength (230.3 ± 41.2 kg) and the VJ (30.8 ± 3.8 cm) were not significantly correlated with RE. One concludes that the maximal aerobic power can explain in part the inter-individual RE variability in endurance athletes. However, maximal isotonic strength and explosive strength seem not to be associated with RE values observed in this group of athletes.

O limiar de esforço percebido (LEP) corresponde à potência crítica e a um indicador de máximo estado estável de consumo de oxigěnio

The perceived exertion has been a target of several investigations, many times with association with objective physiological indicators in exercise. Recently, the identification of the perceived exertion threshold (PET) was proposed in the water running, which presented no difference in relation to the critical velocity. Theoretically, both parameters would be indicators of the maximum steady state of variables such as V̇O2 and blood lactate. The objective of this work was to verify the coincidence between PET, critical power (PCrit) and an indicator of maximum V̇O2 steady state (PCrit') in cycle ergometer. Eight male participants were submitted to progressive effort test in order to determine V̇O2peak (46.7 ± 8.5 ml/kg/min) and to four rectangular tests until exhaustion for the estimation of the critical power model parameters, PET and PCrit'. The hyperbolic relation between mechanical power and time spent for the V̇O2peak to be reached in each test was used for the PCrit' estimation, considered as the asymptote in the power axis, and the portion of the anaerobic work capacity (CTAnaer) depleted up to the establishment of the V̇O2peak (CTAnaer'). In order to identify PET, the straight lines angular coefficients of the perceived exertion in time (ordinate) and the powers used (abscissa) were adjusted to a linear function that provided a point in the power axis in which the perceived exertion would be kept indefinitely stable. The parameters PCrit and CTAnaer were estimated by means of the power-time non-linear equation. In order to compare the estimations of PET, PCrit and PCrit', the analysis of variance ANOVA for repeated measurements was employed, and the associations were established through the Pearson correlation. CTAnaer and CTAnaer' were compared through the t test. PET (180 W ± 61 W), PCrit (174 W ± 43 W) and PCrit' (176 W ± 48 W) were not significantly different and the correlations were of 0.92-0.98. CTAnaer' (14,080 ± 5,219 J) was lower than CTAnaer (22,093 ± 9,042 J). One concludes that the PET predicts the intensity of PCrit and PCrit' with accuracy.

Intervalo hídrico ótimo e produtividade de cultivares de soja

The objective of this study was to determine the optimal water interval and its relationship with soybean yield. For this study, an experiment was carried out in Jaboticabal, São Paulo State, Brazil, in a Haplustox medium textured soil. The experimental design consisted of a completely randomized with subdivided plots (six compaction levels and four soybean cultivars), with four replications. The soil compaction levels were: T 0 = 0, T 1* = 1, T 1 = 1, T 2 = 2, T 4 = 4 and T 6 = 6 strides of a 11 ton tractor, on the same place, side by side on soil surface. In T 1* the compaction occurred when the soil was dry. In December, 2003 the soybean (Glycine max), cultivars IAC Foscarin 31, MG/BR 46 (Conquista), BRS/MG 68 (Vencedora) and IAC 8 - 2 were sown. After sowing, indeformed soil samples were collected in layers of 0.03-0.06, 0.08-0.11, 0.15-0.18 and 0.22-0.25 m for determination of soil water content and resistance to penetration curve and the optimal water content. According to model, the critical soil bulk density (Dsc) for soybean yield varied between cultivars from 1.56 to 1.64 Mg m -3. The soil bulk density at which the yield of soybean cultivars decreased was greater than the Dsc.

Compactação do solo e intervalo hídrico ótimo no crescimento da parte aérea e produtividade da cultura do milho

The aim of this work was to evaluate the effect of soil compaction caused by tractor wheel traffic on the limiting water range (LLWR), shoot growth and levels of compaction and four replications in a completely randomized experimental design. Soil samples with preserved structure were collected in the layers: 0.02-0.05; 0.08-0.11; 0.15-0.18 and 0.22-0.25m to determine macroporosity, microporosity, total porosity, bulk density, resistance to penetration and LLWR. The evaluated corn parameters were: plant and first spike height, steam diameter, number of spikes per plant, plant dry matter, dry matter of 1000 seeds and seed productivity. The soil compaction restricted all corn parameters except the number of spikes per plant and dry matter of 1000 seeds. The LLWR was reduced by the soil resistance to penetration, even in the tilled soil with bulk density of 1.12 Mg m-3. Only the treatment with 11 Mg tractor, repeated four times on the area, demonstrated bulk density above critical bulk density in the LLWR that was 1.37 Mg m-3, where the seed productivity was significantly smaller.

A resolução do problema de despacho ótimo de reativos pelo método da função Lagrangiana-barreira relaxada

This work presents the application of the relaxed barrier-Lagrangian function method to the optimal reactive dispatch problem, which is a nonlinear nonconvex and large problem. In this approach the inequality constraints are treated by the association of modified barrier and primal-dual logarithmic barrier method. Those constraints are transformed in equalities through positive auxiliary variables and are perturbed by the barrier parameter. A Lagrangian function is associated to the modified problem. The first-order necessary conditions are applied generating a non-linear system which is solved by Newton's method. The auxiliary variables perturbation result in an expansion of the feasible set of the original problem, allowing the limits of the inequality constraints to be reach. Numeric tests with the systems CESP 53 buses and the south-southeast Brazilian and the comparative test with the primal-dual logarithmic barrier method indicate that presented method is efficient in the resolution of optimal reactive dispatch problem.

Diferentes procedimentos na determinação de indicadores internos para estimativa de produção fecal e fluxo duodenal de matéria seca em bovinos

Times of in situ incubation (144 and 288h) for determination of internal markers IADF and INDF and the effects of differents procedures (wash or not the nylon bag every 72h incubation) was evaluated in samples of diet, duodenal digesta and cattle feces. The duodenal flow dry matter and fecal production utilizing the internal markers to compare with the external marker chromium oxide there was estimated. The animals were fed with sorgum silage, concentrate or urea. In this experiment, a latin square design was used, in a factorial scheme (two times of incubation × two processing nylon bag). No was observed effect of the incubation time or processing in the internal markers INDF and IADF concentration and the in situ incubation after 144h is adequate to reproduce the indigestible markers fraction in samples. For fecal production estimation, the external marker chromium oxide presented similar result (1.26 kg day-1) as the total fecal collection (1.49 kg day-1). Both the internal markers overestimate the duodenal flow dry matter when compared with the external marker chromium oxide.