Developing correlative Optical-Photoacustic microscopy for high-resolution in vivo imaging

PhotoAcoustic Imaging (PAI) is a branch in clinical and pre-clinical imaging, that refers to the techniques mapping acoustic signals caused by the absorption of the short laser pulse. This conversion of electromagnetic energy of the light to the mechanical (acoustic) energy is usually called photoacoustic e���ect. PAI, by combining optical excitation with acoustical detection, is able to preserve the di���raction limited spatial resolution. At the same time, the penetration depth is extended beyond the di���usive limit. The Laser-Scanning PhotoAcoustic Microscope system (LS-PAM) has been developed, that o���ers the axial resolution of 7.75 ��m with the lateral resolution better than 10 ��m. The ���rst in vivo imaging experiments were carried out. Thus, in vivo label-free imaging of the mouse ear was performed. The principle possibility to image vessels located in deep layers of the mouse skin was shown. As well as that, a gold printing sample, vasculature of the Chick Chorioallantoic Membrane Assay, Drosophila larvae were imaged by PAI. During the experimental work, a totally new application of PAM was found, in which the acoustic waves, generated by incident light can be used for further imaging of another sample. In order to enhance the performance of the presented system two main recommendation can be o���ered. First, the current system should be transformed into re���ection-mode setup system. Second, a more powerful source of light with the su���cient repetition rate should be introduced into the system.

Higher PLIN5 but not PLIN3 content in isolated skeletal muscle mitochondria following acute in vivo contraction in rat hindlimb

Contraction-mediated lipolysis increases the association of lipid droplets and mitochondria, indicating an important role in the passage of fatty acids from lipid droplets to mitochondria in skeletal muscle. PLIN3 and PLIN5 are of particular interest to the lipid droplet���mitochondria interaction because PLIN3 is able to move about within cells and PLIN5 associates with skeletal muscle mitochondria. This study primarily investigated: 1) if PLIN3 is detected in skeletal muscle mitochondrial fraction; and 2) if mitochondrial protein content of PLIN3 and/or PLIN5 changes following stimulated contraction. A secondary aim was to determine if PLIN3 and PLIN5 associate and whether this changes following contraction. Male Long Evans rats (n = 21;age, 52 days; weight = 317 6 g) underwent 30 min of hindlimb stimulation (10 msec impulses, 100 Hz/3 sec at 10���20 V; train duration 100 msec). Contraction induced a ~50% reduction in intramuscular lipid content measured by oil red-O staining of red gastrocnemius muscle. Mitochondria were isolated from red gastrocnemius muscle by differential centrifugation and proteins were detected by western blotting. Mitochondrial PLIN5 content was ~1.6-fold higher following 30 min of contraction and PLIN3 content was detected in the mitochondrial fraction, and unchanged following contraction. An association between PLIN3 and PLIN5 was observed and remained unaltered following contraction. PLIN5 may play a role in mitochondria during lipolysis, which is consistent with a role in facilitating/regulating mitochondrial fatty acid oxidation. PLIN3 and PLIN5 may be working together on the lipid droplet and mitochondria during contraction-induced lipolysis.

Bacterias acidol��cticas como promotoras de la digestibilidad in vitro e in vivo

Tesis (Maestr��a en Ciencias en Producci��n Animal) UANL

Efecto in vivo de diversas antracenonas dim��ricas sobre peroxisomas de Candida boidinii

Tesis (Maestr��a en Ciencias con Especialidad en Quimica Anal��tica Biom��dica) UANL

Estudios in vivo e in vitro de la sensibilidad de actinomadura madurae frente a diversos antimicrobianos de reciente desarrollo

Tesis (Maestr��a en Ciencias con Especialidad en Microbiolog��a M��dica) UANL

Cambios morfol��gicos en la membrana plasm��tica del espermatozoide inducidos in vivo por secreciones de endometrio y c��rvix humanos

Tesis (Maestr��a en Ciencias con Especialidad en Biolog��a de la Reproducci��n) UANL

Actividad de caspofungina contra P. boydii: estudios in vitro e in vivo

Tesis (Maestro en Ciencias con orientaci��n en Microbiolog��a M��dica) U.A.N.L., 2006.

��Es el gen humano hPL-1 un pseudogen? an��lisis de su expresi��n in vivo usando t��cnicas de ingenier��a gen��tica y cultivo celular por Ramiro Ram��rez Sol��s

Tesis (Maestr��a en Ciencias) U.A.N.L.

Determinaci��n de la expresi��n in vivo e in vitro de los receptores TLR-4 y -5 durante la infecci��n por Helicobacter pylori

Tesis (Maestr��a en ciencias con orientaci��n en Microbiolog��a m��dica) U.A.N.L. Facultad de Medicina, 2006.

Evaluaci��n invitro e in vivo de las caracter��sticas de liberaci��n de gabapentina a partir de biomateriales obtenidos v��a sol-gel.

Tesis (Maestr��a en Ciencias con Orientaci��n en Farmacia) UANL, 2011.

Efecto hipotensor e inhibici��n de la actividad de la enzima convertidora de angiotensina I de extractos de semillas de salvia hisp��nica L. in vitro e in vivo.

Tesis (Maestr��a en Ciencias en Nutrici��n) UANL, 2011.

Estudio in vivo de oseointegraci��n en implantes dentales de Ti-6AI-4V con superficies modificadas.

Tesis (Maestro en Ciencias de la Ingenier��a Mec��nica con Especialidad en Materiales) UANL, 2013.

Signaling of angiotensin II-induced vascular protein synthesis in conduit and resistance arteries in vivo

Affiliation: Facult�� de pharmacie, Universit�� de Montr��al & Institut de recherches cliniques de Montr��al

Efectos in vivo e in vitro del clofibrato y del ��cido clof��brico sobre el contenido de vimentina y desmina del citoesqueleto de miocardiocitos de rata

Tesis (Doctorado en Ciencias Biol��gicas) UANL

Detecci��n e identificaci��n de los metabolitos de la T-514 del g��nero karwinskia in vivo e in vitro

Tesis (Doctorado en Ciencias con Especialidad en Farmacolog��a y Toxicolog��a) UANL