939 resultados para Continuously Stirred Bioreactor
Resumo:
This article presents a new method for acquiring three-dimensional (3-D) volumes of ultrasonic axial strain data. The method uses a mechanically-swept probe to sweep out a single volume while applying a continuously varying axial compression. Acquisition of a volume takes 15-20 s. A strain volume is then calculated by comparing frame pairs throughout the sequence. The method uses strain quality estimates to automatically pick out high quality frame pairs, and so does not require careful control of the axial compression. In a series of in vitro and in vivo experiments, we quantify the image quality of the new method and also assess its ease of use. Results are compared with those for the current best alternative, which calculates strain between two complete volumes. The volume pair approach can produce high quality data, but skillful scanning is required to acquire two volumes with appropriate relative strain. In the new method, the automatic quality-weighted selection of image pairs overcomes this difficulty and the method produces superior quality images with a relatively relaxed scanning technique.
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The nanocrystalline (nc) formation was studied in cobalt (a mixture of c (hexagonal close packed) and gamma (face-centered cubic) phases) subjected to surface mechanical attrition treatment. Electron microscopy revealed the operation of {10(1) over bar 0}< 11(2) over bar 0 > prismatic and {0001}< 11(2) over bar 0 > basal slip in the E phase, leading to the successive subdivision of grains to nanoscale. In particular, the dislocation splitting into the stacking faults was observed to occur in ultrafine and nc grains. By contrast, the planar dislocation arrays, twins and martensites were evidenced in the gamma phase. The strain-induced gamma ->epsilon martensitic transformation was found to progress continuously in ultrafine and nc grains as the strain increased. The nc formation in the gamma phase was interpreted in terms of the martensitic transformation and twinning.
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A probe utilizing the bipolar pulse method to measure the density of a conducting fluid has been developed. The probe is specially designed such that the concentration of a stream tube can be sampled continuously. The density was determined indirectly from the measurement of solution conductivity. The probe was calibrated using standard NaCl solutions of varying molarity and was able to rapidly determine the density of a fluid with continuously varying conductance. Measurements of the conductivity profiles, corresponding density profiles, and their fluctuation levels are demonstrated in a channel flow with an electrolyte injected from a slot in one wall.
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A temperature-controlled pool boiling (TCPB) device has been developed to study the bubble behavior and heat transfer in pool boiling phenomenon both in normal gravity and in microgravity. A thin platinum wire of 60 mu m in diameter and 30 mm in length is simultaneously used as heater and thermometer. The fluid is R113 at 0.1 MPa and subcooled by 26 degrees C nominally for all cases. Three modes of heat transfer, namely single-phase natural convection, nucleate boiling, and two-mode transition boiling, are observed in the experiment both in microgravity aboard the 22nd Chinese recoverable satellite and in normal gravity on the ground before and after the space flight. Dynamic behaviors of vapor bubbles observed in these experiments are reported and analyzed in the present paper. In the regime of fully developed nucleate boiling, the interface oscillation due to coalescence of adjacent tiny bubbles is the primary reason of the departure of bubbles in microgravity. On the contrary, in the discrete bubble regime, it's observed that there exist three critical bubble diameters in microgravity, dividing the whole range of the observed bubbles into four regimes. Firstly, tiny bubbles are continually forming and growing on the heating surface before departing slowly from the wire when their sizes exceed some value of the order of 10(-1) mm. The bigger bubbles with about several millimeters in diameter stay on the wire, oscillate along the wire, and coalesce with adjacent bubbles. The biggest bubble with diameter of the order of 10 mm, which was formed immediately after the onset of boiling, stays continuously
Resumo:
Fatigue testing was conducted using a kind of triangular isostress specimen to obtain the short-fatigue-crack behaviour of a weld low-carbon steel. The experimental results show that short cracks continuously initiate at slip bands within ferrite grain domains and the crack number per unit area gradually increases with increasing number of fatigue cycles. The dispersed short cracks possess an orientation preference, which is associated with the crystalline orientation of the relevant slip system. Based on the observed collective characteristics, computer modelling was carried out to simulate the evolution process of initiation, propagation and coalescence of short cracks. The simulation provides progressive displays which imitate the appearance of experimental observations. The results of simulation indicate that the crack path possesses a stable value of fractal dimension whereas the critical value of percolation covers a wide datum band, suggesting that the collective evolution process of short cracks is sensitive to the pattern of crack site distribution.
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In this paper, a damage function defined by the residual strength of spalled specimens of an aluminium alloy is given to characterize the spallation of the material. Based on this function a simple method for continuously describing the spallation may be developed. Stress wave profiles showing the signal of spallation were successfully obtained with carbon gauges. Microscopic observations of the spalled aluminium alloy specimens reveal that the nucleation of spallation initiates from cracking of the second phase particles. Spallation is a process of crack nucleation, growth and coalescence to final, complete disintegration.
Resumo:
通过高压扭转对铜试样施加不同程度的变形,研究了样品扭转面(ND面)和纵截面(TD面)上微观组织特征.对ND面,在较小的剪应变下,原始晶粒形貌模糊,晶粒内部形成等轴状的位错胞及亚晶结构;随变形量的增大,亚晶间取向差及亚晶内部的位错密度增大,最后形成亚微米尺度的等轴晶粒.对TD面,变形初期原始晶粒被拉长,晶粒内部为位错墙分割成的层状结构,层内为拉长的位错胞;随变形程度的增大,拉长晶粒的宽度减小,与剪切方向的夹角减小,晶内层状组织间距减小,并逐渐演化成拉长的亚晶组织;进一步增大变形,晶粒拉长痕迹消失,变形组织与ND面相似,为等轴状亚微米晶粒.压缩实验表明,经16圈扭转后,整个试样上的压缩性能基本均匀,σ0.2达到385MPa,应变率敏感性指数增大至0.021.
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[ES] En el entorno actual, la obtención de innovaciones en la gestión, en el producto y en el proceso, resulta una herramienta competitiva fundamental para las empresas. Éstas, para garantizarse fuentes de innovación, deben recurrir a mecanismos de captación y generación de conocimiento. Un mecanismo fundamental a este respecto es la captación de conocimientos del exterior, en especial los de tipo científico y tecnológico.
Resumo:
Apresenta a urna eletrônica como agente essencial para a manutenção da democracia. São apresentadas as origens do voto no Brasil, indo até a proposta e execução do ambicioso projeto de eleições gerais com base em urnas eletrônicas. Descreve como se deu o desenvolvimento do equipamento. Os requisitos de segurança da informação são apresentados no contexto da votação eletrônica tendo como parâmetro a norma ISO 27.002. Observa que há melhorias que devem ser continuamente realizadas a fim de garantir a efetiva realização de um processo eleitoral seguro e confiável.
Resumo:
A crescente demanda por informações e, principalmente, o aumento exponencial de redes conectadas à Rede Mundial de Computadores (Internet), faz com que as organizações tenham que modernizar sua infraestrutura constantemente. O esgotamento do endereçamento disponível desta rede faz com que essas organizações estudem a implantação da nova versão do protocolo da Internet, o IPv6. A Câmara dos Deputados, como grande provedor de informações, e procurando manter-se conectada ao maior número de usuários possível, vê a necessidade de implantação de novas soluções em seu ambiente, mas por ser um ambiente muito complexo, e por ter diversas dependências necessita da utilização dos recursos e artefatos de gerenciamento de projetos para tornar possível a implantação de IPv6 em sua rede corporativa.
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Following the quantitative determination of dust cloud parameters, this study investigates the flame propagation through cornstarch dust clouds in a vertical duct of 780 mm height and 160 x 160 mm square cross section, and gives particular attention to the effect of small scale turbulence and small turbulence intensity on flame characteristics. Dust suspensions in air were produced using an improved apparatus ensuring more uniform distribution and repeatable dust concentrations in the testing duct. The dispersion-induced turbulence was measured by means of a particle image velocimetry (PIV) system, and dust concentrations were estimated by direct weighing method. This quantitative assessment made it possible to correlate observed flame behaviors with the parameters of the dust cloud. Upward propagating dust flames, from both closed/open bottom end to open/closed top end of the duct, were visualized by direct light and shadow photography. From the observation of propagation regimes and the measurements of flame velocity, a critical value of the turbulence intensity can be specified below which laminar flame propagation would be established. This transition condition was determined to be 10 cm/s. Laminar flames propagated with oscillations from the closed bottom end to the open top end of the testing duct, while the turbulent flames accelerated continuously. Both laminar and turbulent flames propagated with steady velocity from the open bottom end to the closed top end of the duct. The measured propagation velocity of laminar flames appeared to be in the range of 0.45-0.56 m/s, and it was consistent with the measurements reported in the literature. In the present experimental study, the influence of dust concentration on flame propagation was also examined, and the flame propagation velocity was found weakly sensitive to the variations in dust concentration. Some information on the flame structure was revealed from the shadow records, showing the typical heterogeneous feature of the dust combustion process.
Resumo:
The nanocrystalline (nc) formation was studied in cobalt (a mixture of c (hexagonal close packed) and gamma (face-centered cubic) phases) subjected to surface mechanical attrition treatment. Electron microscopy revealed the operation of {10(1) over bar 0}< 11(2) over bar 0 > prismatic and {0001}< 11(2) over bar 0 > basal slip in the E phase, leading to the successive subdivision of grains to nanoscale. In particular, the dislocation splitting into the stacking faults was observed to occur in ultrafine and nc grains. By contrast, the planar dislocation arrays, twins and martensites were evidenced in the gamma phase. The strain-induced gamma ->epsilon martensitic transformation was found to progress continuously in ultrafine and nc grains as the strain increased. The nc formation in the gamma phase was interpreted in terms of the martensitic transformation and twinning.
Resumo:
During October, 1972 the Patuxent River Estuary was monitored intensively and synoptically over two tidal cycles to determine the spatial and temporal patterns of various hydrodynamic, chemical and biological features. Forty-one depths at eleven stations along nine transects were sampled simultaneously at hourly intervals for salinity, temperature, dissolved oxygen, chlorohyll a, particulate nitrogen, nitrate, nitrite, total kjeldahl nitrogen, ammonia, particulate carbohydrate, dissolved organic carbon, total hydrolizable phosphorous, dissolved inorganic phosphorous, suspended sediment, particle size distribution, and zooplankton. Tidal velocity was continuously monitored at each depth by recording current meters. Riverine input and meteorological conditions were relatively stable for two weeks preceeding the deployment. This communication describes the calculation of the intrinsic rates of change of the observed variables from their measured distributions in the Estuary. The steady-state, one-dimensional equation of species continuity is employed to separate the advection and tidal dispersion of a hydrodynamically passive substance frbm its intrinsic rate of change at point. A new spatial transform is introduced for the purpose of interpolation and extrapolation of data.The intrinsic rate of change profiles reveal a region of heavy bloom activity in the upper estuary and a secondary bloom near the point in the River that most of the suspended material settles out. The changes in ammonia and nitrates are highly correlated to the productivity patterns. Phosphorous rates are less closely correlated to productivity. The perturbations that the Chalk Point steam electric power plant have on the heat and oxygen balances are easily discernible.
Resumo:
The spray of emulsified fuel, composed of diesel fuel, water and methanol can make micro-explosion under high temperature conditions, and the viscosity and the atomization characteristics of emulsion have significant effects on the micro- explosion of emulsions. To clarify the combustion mechanism of water-in-oil emulsion sprays, combustion bomb experiments were carried out, and the droplet group micro- explosions in W/O fuel emulsion sprays in a high-pressure, high-temperature bomb were observed clearly by a multi-pulsed, off-axis, image-plane ruby laser holocamera and continuously by a high-speed CCD camera.The viscosity and atomization characteristics of emulsions were also studied experimentally. The experimental results show that the higher concentration of the aqueous phase (water-methanol) (<50%) increases the viscosity of the emulsions, especially for higher agent concentration, and higher aqueous phase concentration and higher viscosity results in lager Sauter Mean Diameter (SMD). The experiment results also show that the different kinds of emulsifying agents, with different Hydrophile-Lipophile Balance (HLB) values, have significant influence on the viscosity of the emulsions.
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Assateague Island is an offshore bar comprising the south-eastern coast of Maryland and the northeastern coast of Virgina. It is part of the system of discontinuous barrier reefs or bars which occupy most of the Atlantic shoreline from Florida to Massachusetts. These are unstable bars, continuously influenced by storm winds and tides which provide a distinct and rigorous habitat for the vegetation there. General floras of the Delmarva Peninusla do not mention Assateague Island specifically. The objective is to prepare a catalog of the vascular plants of Assateague Island and to describe the communities in which they are found, in the hope it will add to the knowledge of barrier reef vegetation.