The "Beef" about Beef

The grading of beef has become a controversial issue, with both government and the cattle industry working to ensure uniformity. The author contends that meat quality, and the resulting tenderness, juiciness, and flavor desired by consumers, is being threatened by such pressures and actions.

Increasing beef production won’t reduce emissions

BP is supported by a grant from CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) through the Brazilian Science without Borders programme (number 88881.068115/2014-01). He thanks Luciana Leite de Araújo for suggesting the topic of this letter.

Increasing beef production won’t reduce emissions

BP is supported by a grant from CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) through the Brazilian Science without Borders programme (number 88881.068115/2014-01). He thanks Luciana Leite de Araújo for suggesting the topic of this letter.

A Loop-mediated Isothermal Amplification Method for Rapid Direct Detection and Differentiation of Non-pathogenic and Verocytotoxigenic Escherichia coli in Beef and Bovine Faeces

The aim of this study was to develop a multiplex loop-mediated isothermal amplification (LAMP) method capable of detecting Escherichia coli generally and verocytotoxigenic E. coli (VTEC) specifically in beef and bovine faeces. The LAMP assay developed was highly specific (100%) and able to distinguish between E. coli and VTEC based on the amplification of the phoA, and stx1 and/or stx2 genes, respectively. In the absence of an enrichment step, the limit of detection 50% (LOD50) of the LAMP assay was determined to be 2.83, 3.17 and 2.83-3.17 log CFU/g for E. coli with phoA, stx1 and stx2 genes, respectively, when artificially inoculated minced beef and bovine faeces were tested. The LAMP calibration curves generated with pure cultures, and spiked beef and faeces, suggested that the assay had good quantification capability. Validation of the assay, performed using retail beef and bovine faeces samples, demonstrated good correlation between counts obtained by the LAMP assay and by a conventional culture method, but suggested the possibility of false negative LAMP results for 12.5-14.7% of samples tested. The multiplex LAMP assay developed potentially represents a rapid alternative to culture for monitoring E.coli levels in beef or faeces and it would provide additional information on the presence of VTEC. However, some further optimisation is needed to improve detection sensitivity.

Johne’s Disease-Beef Fall 2009, Vol. 1, no.2

Animal science newsletter about Johne's Disease in cattle. Statistics, treatments, information about the disease itself and more.

Johne’s Disease-Beef, Fall 2011, Vol. 4, no.1

Animal science newsletter about Johne's Disease in cattle. Statistics, treatments, information about the disease itself and more.