Análisis prospectivo del despliegue de redes de comunicaciones móviles LTE en nuevas bandas de frecuencia mediante el desarrollo de modelos tecnoeconómicos

El estándar LTE se ha posicionado como una de las claves para que los operadores de telecomunicación puedan abordar de manera eficiente en costes el crecimiento de la demanda de tráfico móvil que se prevé para los próximos años, al ser una tecnología más escalable en el núcleo de la red y más flexible en la interfaz radio que sus predecesoras. En este sentido, es necesario también que los reguladores garanticen un acceso al espectro radioeléctrico adecuado, equitativo y no discriminatorio, que permita un entorno estable para el despliegue de redes de comunicaciones móviles avanzadas. Además de la flexibilización del marco regulador del espectro radioeléctrico en Europa, que ha permitido el despliegue de nuevas tecnologías en las bandas de frecuencia históricas de GSM, se ha puesto a disposición espectro adicional para sistemas IMT en nuevas bandas de frecuencia, lo que ha planteando a su vez nuevos retos para la tecnología y la regulación. La fragmentación del espectro disponible para comunicaciones móviles ha impulsado el desarrollo de técnicas de agregación de portadoras en las nuevas versiones del estándar LTE, que permiten explotar mejor los recursos radio en su conjunto. No obstante, el espectro inferior a 1 GHz sigue siendo escaso, ya que el tráfico móvil aumenta y la banda de 900 MHz aún se utiliza para servicios GSM, lo que no ha conseguido sino agravar la disputa entre los servicios de radiodifusión terrestre y de comunicaciones móviles por la parte superior de la banda UHF. En concreto, la banda de 700 MHz se perfila como una de las próximas para aumentar el espectro disponible para los servicios en movilidad, si bien su liberación por parte de las actuales redes de Televisión Digital Terrestre presenta no pocas dificultades en los Estados miembros en los que ésta es la principal plataforma audiovisual de acceso gratuito, abriendo un debate sobre el modelo audiovisual a largo plazo en Europa. Por otro lado, las políticas públicas de promoción del acceso a la banda ancha rápida y ultrarrápida de la presente década han establecido objetivos ambiciosos para el año 2020, tanto en el ámbito europeo como en los diferentes Estados miembros. La universalización del acceso a redes de banda ancha de al menos 30 Mbps constituye uno de los principales retos. Las expectativas generadas por la tecnología LTE y la puesta a disposición de nuevas bandas de frecuencia hace posible que los servicios de acceso fijo inalámbrico adquieran especial relevancia ante los objetivos de política pública establecidos que, como ha sido reconocido en diversas ocasiones, no podrán lograrse sino con un compendio de diferente tecnologías. Para esta Tesis Doctoral se han desarrollado una serie modelos tecnoeconómicos con el objetivo de realizar un análisis prospectivo que evalúa tres casos de especial relevancia en el despliegue de redes LTE: en primer lugar, la valoración económica de la banda de 700 MHz; en segundo lugar, la evaluación de modelos de negocio y reducción de costes considerando tecnologías femtocelulares; y finalmente, la viabilidad de las redes LTE de acceso fijo inalámbrico para el cierre de la brecha digital en el acceso a la banda ancha de 30 Mbps. En relación con la aplicación del análisis tecnoeconómico para la valoración del espectro de 700 MHz, los resultados obtenidos ponen de manifiesto dos cuestiones fundamentales. En primer lugar, la necesidad de asignar a los operadores más espectro para satisfacer las previsiones de demanda de tráfico móvil a medio plazo. En segundo, existe una diferencia notable en los costes de despliegue de una red LTE cuando se dispone de espectro en frecuencias inferiores a 1 GHz y cuando no, pero esta diferencia de costes disminuye a medida que se añade nuevo espectro sub-1GHz. De esta manera, la atribución de la banda de 700 MHz a servicios de comunicaciones móviles supone una reducción relevante en los costes de despliegue si el operador no dispone de espectro en la banda de 800 MHz, pero no así si ya dispone de espectro en bandas bajas para el despliegue. En este sentido, puede concluirse que el precio que los operadores estarán dispuestos a pagar por el espectro de la banda de 700 MHz dependerá de si ya tienen disponible espectro en la banda de 800 MHz. Sin embargo, dado que la competencia por ese espectro será menor, los ingresos esperables en las licitaciones de esta nueva banda serán en general menores, a pesar de que para algunos operadores este espectro sería tan valioso como el de 800 MHz. En segundo lugar, en relación con el despliegue de femtoceldas pueden extraerse algunas conclusiones en términos de ahorro de costes de despliegue y también de cara a la viabilidad de los modelos de negocio que posibilitan. El ahorro que supone la introducción de femtoceldas en el despliegue de una red LTE frente al caso de un despliegue exclusivamente macrocelular se ha demostrado que es mayor cuanto menor es el ancho de banda disponible para la red macrocelular. En esta línea, para un operador convergente el despliegue de femtoceldas tiene sentido económico si el ancho de banda disponible es escaso (en torno a 2x10 MHz), que, en el caso de España, puede reflejar el caso de los operadores del segmento fijo que son nuevos entrantes en el móvil. Por otro lado, los modelos de acceso abierto son interesantes para operadores exclusivamente móviles, porque consiguen flexibilizar los costes sustituyendo estaciones base macrocelulares por el despliegue de femtoceldas, pero necesitan desplegarse en zonas con una densidad de población relativamente elevada para que éstas descarguen tráfico de varios usuarios de la red macrocelular simultáneamente. No obstante, las femtoceldas son beneficiosas en todo caso si es el usuario quien asume los costes de la femtocelda y el backhaul, lo que sólo parece probable si se integran en el modelo de negocio de comercialización de nuevos servicios. Por tanto, el despliegue de femtoceldas en buena parte de la casuística estudiada sólo tiene sentido si consiguen aumentar los ingresos por usuario comercializando servicios de valor añadido que necesiten calidad de servicio garantizada y exploten a la vez de esa forma su principal ventaja competitiva respecto a la tecnología WiFi. Finalmente, en relación con el papel de la tecnología LTE para la provisión de servicios de acceso fijo inalámbrico para la banda ancha de 30 Mbps, se ha desarrollado un modelo TD-LTE y mediante la metodología de análisis tecnoeconómico se ha realizado un estudio prospectivo para el caso de España. Los resultados obtenidos preciden una huella de cobertura de FTTH del 74% para 2020, y demuestran que una red TD-LTE en la banda de 3,5 GHz resulta viable para aumentar la cobertura de servicios de 30 Mbps en 14 puntos porcentuales. Junto con la consideración de la cobertura de otras redes, la cobertura de 30 Mbps de acuerdo a la viabilidad de los despliegues alcanzaría el 95% en España en el año 2020. Como resumen, los resultados obtenidos muestran en todos los casos la capacidad de la tecnología LTE para afrontar nuevos desafíos en relación con el aumento del tráfico móvil, especialmente crítico en las zonas más urbanas, y el cierre de la brecha digital en el acceso a la banda ancha rápida en las zonas más rurales. ABSTRACT The LTE standard has been pointed out as one of the keys for telecom operators to address the demand growth in mobile traffic foreseen for the next years in a cost-efficient way, since its core network is more scalable and its radio interface more flexible than those of its predecessor technologies. On the other hand, regulators need to guarantee an adequate, equitable and non-discriminatory access to radio spectrum, which enable a favorable environment for the deployment of advanced mobile communication networks. Despite the reform of the spectrum regulatory framework in Europe, which allowed for the deployment of new technologies in the historic GSM bands, additional spectrum has been allocated to IMT systems in new frequency bands, what in turn has set out new challenges for technology and regulation. The current fragmentation of available spectrum in very different frequency bands has boosted the development of carrier aggregation techniques in most recent releases of the LTE standard, which permit a better exploitation of radio resources as a whole. Nonetheless, spectrum below 1 GHz is still scarce for mobile networks, since mobile traffic increases at a more rapid pace than spectral efficiency and spectrum resources. The 900 MHz frequency band is still being used for GSM services, what has worsen the dispute between mobile communication services and terrestrial broadcasting services for the upper part of the UHF band. Concretely, the 700 MHz frequency band has been pointed out as one of the next bands to be allocated to mobile in order to increase available spectrum. However, its release by current Digital Terrestrial Television networks is challenging in Member States where it constitutes the main free access audiovisual platform, opening up a new debate around the audiovisual model in the long term in Europe. On the other hand, public policies of the present decade to promote fast and ultrafast broadband access has established very ambitious objectives for the year 2020, both at European and national levels. Universalization of 30 Mbps broadband access networks constitutes one of the main challenges. Expectations raised by LTE technology and the allocation of new frequency bands has lead fixed wireless access (FWA) services to acquire special relevance in light of public policy objectives, which will not be met but with a compendium of different technologies, as different involved stakeholders have acknowledged. This PhD Dissertation develops techno-economic models to carry out a prospective analysis for three cases of special relevance in LTE networks’ deployment: the spectrum pricing of the 700 MHz frequency band, an assessment of new business models and cost reduction considering femtocell technologies, and the feasibility of LTE fixed wireless access networks to close the 30 Mbps broadband access gap in rural areas. In the first place and regarding the application of techno-economic analysis for 700 MHz spectrum pricing, obtained results reveal two core issues. First of all, the need to allocate more spectrum for operators in order to fulfill mobile traffic demand in the mid-term. Secondly, there is a substantial difference in deployment costs for a LTE network when there is sub-1GHz spectrum available and when there is not, but this difference decreases as additional sub-1GHz spectrum is added. Thus, the allocation of 700 MHz band to mobile communication services would cause a relevant reduction in deployment costs if the operator does not count on spectrum in the 800 MHz, but not if it already has been assigned spectrum in low frequencies for the deployment. In this regard, the price operators will be willing to pay for 700 MHz spectrum will depend on them having already spectrum in the 800 MHz frequency band or not. However, since competition for the new spectrum will not be so strong, expected incomes from 700 MHz spectrum awards will be generally lower than those from the digital dividend, despite this spectrum being as valuable as 800 MHz spectrum for some operators. In the second place, regarding femtocell deployment, some conclusions can be drawn in terms of deployment cost savings and also with reference to the business model they enable. Savings provided by a joint macro-femto LTE network as compared to an exclusively macrocellular deployment increase as the available bandwidth for the macrocells decreases. Therefore, for a convergent operator the deployment of femtocells can only have economic sense if the available bandwidth is scarce (around 2x10 MHz), which might be the case of fix market operators which are new entrant in mobile market. Besides, open access models are interesting for exclusively mobile operators, since they make costs more flexible by substituting macrocell base stations by femtocells, but they need to be deployed relatively densely populated areas so that they can offload traffic from several macrocell users simultaneously. Nonetheless, femtocells are beneficial in all cases if the user assumes both femtocell and backhaul costs, which only seems probable if they are integrated in a business model commercializing new services. Therefore, in many of the cases analyzed femtocell deployment only makes sense if they increase revenues per user through new added value services which need from guaranteed quality of service, thus exploiting its main competitive advantage compared to WiFi. Finally, regarding the role of LTE technology in the provision of fixed wireless access services for 30 Mbps broadband, a TD-LTE model has been developed and a prospective study has been carried out through techno-economic methodology for the Spanish case. Obtained results foresee a FTTH coverage footprint of 74% households for 2020, and prove that a TD-LTE network in the 3.5 GHz band results feasible to increase 30 Mbps service coverage in additional 14 percentage points. To sum up, obtained results show LTE technology capability to address new challenges regarding both mobile traffic growth, particularly critical in urban zones, and the current digital divide in fast broadband access in most rural zones.

Diseño de un sistema networking audio para instalación audiovisual

Una vez presentada la tecnología de Networking audio (redes de datos, protocolos actuales, etc.) se realizará un diseño de la instalación del sistema de audio, en el que el punto de partida es la parte creativa de la actividad en dicha instalación: un juego en el que la comunicación auditiva es lo fundamental. La instalación se compondrá de una sala central, tres salas de grupos, tres salas de cabinas de actores y ocho salas de pasaje. Esta actividad tan particular hará plantearse configuraciones, equipamiento y formas de trabajar especiales que, mediante la tecnología de audio vía red de datos y el equipamiento auxiliar a esta red, podría realizarse de la una forma óptima cumpliendo con todos los objetivos de la actividad, tanto técnicos como relativos al juego. El libro se dividirá en dos partes: La primera parte consistirá en una explicación de lo que son las redes de datos y los aspectos básicos para entenderlas desde un punto de vista práctico: qué es Ethernet, los componentes de una red... Una vez explicada la terminología específica de redes, se expondrán los protocolos que se usan para transmitir audio profesional a día de hoy. En la segunda parte, se empezará presentando la actividad que se realizará en nuestra instalación: un juego de rol. A continuación se conocerá el flujo de señales existentes para después, poner en práctica lo aprendido en la primera parte: diseñaremos una instalación audiovisual mediante networking audio. Un sistema de estas características necesita además de dispositivos en red, sistemas convencionales de audio. Durante el diseño y debido a las necesidades tan específicas de la instalación, se verá que ha sido necesario pensar en sistemas especiales para hacer posible la actividad para la que ha sido ideada nuestra instalación. Los objetivos de este proyecto son, desarrollar los puntos que tendría que tener en cuenta un integrador que se proponga diseñar un sistema de audio networking para una instalación audiovisual para, a continuación, poner en práctica estos conocimientos con la exposición del diseño de una instalación en la que se llevará a cabo una actividad lúdica y de aprendizaje en la que una óptima transmisión de señal de audio a tiempo real, es lo fundamental. ABSTRACT. Once introduced the Networking technology (data networks, current protocols, etc.), the audio installation design is being done. In which the starting point is the creative part of the activity will be made: one game in which the auditory communication is fundamental. The installation will consist of a central room, three meeting groups, three actor cabins rooms and eight passage rooms. This particular activity will consider configurations, equipment and forms of special working that through audio technology via data network and auxiliary equipment to this network, it could be done in an optimal way to meet all the goals of the activity, both technical and relative to the game. The book is divided into two parts: The first part consists of an explanation of what the data networks and the basics to understand from a practical point of view: what Ethernet is, the network components... Once specific network terminology is explained, the current protocols used to transmit professional audio are being showed. In the second part, it is introducing the activity to be made in our installation: a game. Then, the flow of existing signals are being known, we practice what I learned in the first part: we will design an audiovisual installation by audio networking. A system like this besides networked devices, it needs conventional audio systems. During the design and due to the very specific needs of the installation, you will see that it was necessary to think of special systems for this special activity. The goals of this project are to develop the points that an system integrator would have to consider to design a system of networking audio for an audiovisual installation, then put this knowledge into practice with the installation design where it will take place a fun and learning activity in which an optimal transmission of audio signal in real time, is basic.

DESENVOLVIMENTO DO TEATRO MUSICAL BRASILEIRO À LUZ DA NOTORIEDADE MIDIÁTICA DAS ADAPTAÇÕES E DOS ARTISTAS

O trabalho tem a proposta de analisar os desdobramentos do teatro musical brasileiro desde a primeira encenação em território nacional de adaptações de espetáculos do Teatro de Revista, gênero originário da França, até as superproduções musicais realizadas nos últimos 16 anos de adaptações de espetáculos americanos. O panorama histórico e analítico será estudado, com ênfase no teatro musical que se utiliza de elementos midiatizados para estar inserido em uma sociedade em que a produção cultural é vista como internacionalizada e mercantilizada. Como forma de marketing, os produtores utilizam-se da notoriedade midiática presente em formatos estrangeiros já consagrados, adaptações renomadas e bem aceitas pelo público, além da fama de celebridades que são escaladas para os musicais. Tudo para a conquista de um patrocinador que, por sua vez, acaba fazendo exigências que interferem de maneira decisiva na montagem dos espetáculos. Em meio a um processo onde são tantos os direcionamentos pré-estabelecidos por patrocinadores, onde se encontra o genuíno teatro musical brasileiro? A pesquisa abrange o ineditismo da presença de temáticas nacionais em formatos estrangeiros e agrega o conjunto de fatores que possibilitam que um roteiro de musical saia do papel e adentre os palcos, tais como as políticas públicas de incentivos fiscais; a ligação de empresas patrocinadoras e suas marcas a musicais; o fato de que, mesmo as produções sendo pagas por dinheiro público, possuírem ingressos que não são a preços populares. Para auxiliar nas conjecturas a serem formadas, será utilizada uma metodologia histórico-descritiva com foco na relação do tema com elementos notórios na mídia, como os artistas e obras a serem adaptadas no palco.

Postgastrulation Smad2-deficient embryos show defects in embryo turning and anterior morphogenesis

SMAD2 is a member of the transforming growth factor β and activin-signaling pathway. To examine the role of Smad2 in postgastrulation development, we independently generated mice with a null mutation in this gene. Smad2-deficient embryos die around day 7.5 of gestation because of failure of gastrulation and failure to establish an anterior–posterior (A-P) axis. Expression of the homeobox gene Hex (the earliest known marker of the A-P polarity and the prospective head organizer) was found to be missing in Smad2-deficient embryos. Homozygous mutant embryos and embryonic stem cells formed mesoderm derivatives revealing that mesoderm induction is SMAD2 independent. In the presence of wild-type extraembryonic tissues, Smad2-deficient embryos developed beyond 7.5 and up to 10.5 days postcoitum, demonstrating a requirement for SMAD2 in extraembryonic tissues for the generation of an A-P axis and gastrulation. The rescued postgastrulation embryos showed malformation of head structures, abnormal embryo turning, and cyclopia. Our results show that Smad2 expression is required at several stages during embryogenesis.

Neuronal nicotinic threonine-for-leucine 247 α7 mutant receptors show different gating kinetics when activated by acetylcholine or by the noncompetitive agonist 5-hydroxytryptamine

Mutation of the highly conserved leucine residue (Leu-247) converts 5-hydroxytryptamine (5HT) from an antagonist into an agonist of neuronal homomeric α7 nicotinic acetylcholine receptor expressed in Xenopus oocytes. We show here that acetylcholine (AcCho) activates two classes of single channels with conductances of 44 pS and 58 pS, similar to those activated by 5HT. However, the mean open time of AcCho-gated ion channels (11 ms) is briefer than that of 5HT-gated ion channels (18 ms). Furthermore, whereas the open time of AcCho channels lengthens with hyperpolarization, that of 5HT channels is decreased. In voltage-clamped oocytes, the apparent affinity of the α7 mutant receptor for 5HT is not modified by the presence of dihydro-β-erythroidine, which acts on the AcCho binding site in a competitive manner. This indicates a noncompetitive action of 5HT on nicotinic acetylcholine receptors. Considered together, our findings show that AcCho gates α7 mutant channels with similar conductance but with different kinetic profile than the channels gated by 5HT, suggesting that the two agonists act on different docking sites. These results will help to understand the crosstalk between cholinergic and serotonergic systems in the central nervous system.

Structural assignments to the Mycoplasma genitalium proteins show extensive gene duplications and domain rearrangements

The parasitic bacterium Mycoplasma genitalium has a small, reduced genome with close to a basic set of genes. As a first step toward determining the families of protein domains that form the products of these genes, we have used the multiple sequence programs psi-blast and geanfammer to match the sequences of the 467 gene products of M. genitalium to the sequences of the domains that form proteins of known structure [Protein Data Bank (PDB) sequences]. PDB sequences (274) match all of 106 M. genitalium sequences and some parts of another 85; thus, 41% of its total sequences are matched in all or part. The evolutionary relationships of the PDB domains that match M. genitalium are described in the structural classification of proteins (SCOP) database. Using this information, we show that the domains in the matched M. genitalium sequences come from 114 superfamilies and that 58% of them have arisen by gene duplication. This level of duplication is more than twice that found by using pairwise sequence comparisons. The PDB domain matches also describe the domain structure of the matched sequences: just over a quarter contain one domain and the rest have combinations of two or more domains.

Transgenic Drosophila expressing human amyloid precursor protein show γ-secretase activity and a blistered-wing phenotype

The importance of the amyloid precursor protein (APP) in the pathogenesis of Alzheimer’s disease (AD) became apparent through the identification of distinct mutations in the APP gene, causing early onset familial AD with the accumulation of a 4-kDa peptide fragment (βA4) in amyloid plaques and vascular deposits. However, the physiological role of APP is still unclear. In this work, Drosophila melanogaster is used as a model system to analyze the function of APP by expressing wild-type and various mutant forms of human APP in fly tissue culture cells as well as in transgenic fly lines. After expression of full-length APP forms, secretion of APP but not of βA4 was observed in both systems. By using SPA4CT, a short APP form in which the signal peptide was fused directly to the βA4 region, transmembrane domain, and cytoplasmic tail, we observed βA4 release in flies and fly-tissue culture cells. Consequently, we showed a γ-secretase activity in flies. Interestingly, transgenic flies expressing full-length forms of APP have a blistered-wing phenotype. As the wing is composed of interacting dorsal and ventral epithelial cell layers, this phenotype suggests that human APP expression interferes with cell adhesion/signaling pathways in Drosophila, independently of βA4 generation.

11β-Hydroxysteroid dehydrogenase type 1 knockout mice show attenuated glucocorticoid-inducible responses and resist hyperglycemia on obesity or stress

Glucocorticoid hormones, acting via nuclear receptors, regulate many metabolic processes, including hepatic gluconeogenesis. It recently has been recognized that intracellular glucocorticoid concentrations are determined not only by plasma hormone levels, but also by intracellular 11β-hydroxysteroid dehydrogenases (11β-HSDs), which interconvert active corticosterone (cortisol in humans) and inert 11-dehydrocorticosterone (cortisone in humans). 11β-HSD type 2, a dehydrogenase, thus excludes glucocorticoids from otherwise nonselective mineralocorticoid receptors in the kidney. Recent data suggest the type 1 isozyme (11β-HSD-1) may function as an 11β-reductase, regenerating active glucocorticoids from circulating inert 11-keto forms in specific tissues, notably the liver. To examine the importance of this enzyme isoform in vivo, mice were produced with targeted disruption of the 11β-HSD-1 gene. These mice were unable to convert inert 11-dehydrocorticosterone to corticosterone in vivo. Despite compensatory adrenal hyperplasia and increased adrenal secretion of corticosterone, on starvation homozygous mutants had attenuated activation of the key hepatic gluconeogenic enzymes glucose-6-phosphatase and phosphoenolpyruvate carboxykinase, presumably, because of relative intrahepatic glucocorticoid deficiency. The 11β-HSD-1 −/− mice were found to resist hyperglycamia provoked by obesity or stress. Attenuation of hepatic 11β-HSD-1 may provide a novel approach to the regulation of gluconeogenesis.

O regime jurídico-constitucional da radiodifusão e das telecomunicações no Brasil em face do conceito de atividades audiovisuais : a inconstitucionalidade do anteprojeto de lei que cria a Agência Nacional do Cinema e do Audiovisual - Ancinav

Inclui bibliografia.