The future of bioscience fieldwork in UK higher education

Fieldwork is an important and often enjoyable part of learning in Bioscience degree courses, however it is unclear how the recent reforms to Higher Education (HE) may impact the future funding of outdoor learning. This paper reports on the findings from a recent survey of 30 HE Bioscience practitioners from across the UK. Their current level of fieldwork provision and factors affecting this provision in the future were explored. The data showed that the level of fieldwork had remained similar over the past five years and this was set to remain so over the next academic year and also into the next five years (when it may even increase). Funding of fieldwork was under review in most institutions due to the increase in student tuition fees and it was found that in some cases the cost of compulsory fieldwork will be subsumed within the overall course fee. Many influencing factors were discussed, but the most frequently raised topics were that of the development of employability skills during fieldwork and its importance in attracting and retaining students. Both topics are high on the agenda of HE institutions going forward into the new funding model, suggesting that fieldwork will remain a central part of the Bioscience curriculum.

A Guide to Office Clerical Time Standards

A Guide to Office Clerical Time Standards is an instructional performance piece based on a corporate manual from 1960. The pamphlet is focused on the time necessary for the accomplishment of minute labour procedures in the office, from the depressing and releasing of typewriter keys to the opening and closing of filing cabinet drawers. In the performance, seven costumed performers represent the different levels of management and employment while performing the actions described in the guide, accompanied by a live musical score. There has been much discussion of the changes to work in the west following the decline of post-Fordist service sector jobs. These increasingly emphasise the specificity of employees’ knowledge and cognitive skill. However, this greater flexibility and creativity at work has been accompanied by an opposite trajectory. The proletarisation of white collar work has given rise to more bureaucracy, target assessment and control for workers in previously looser creative professions, from academia to the arts. The midcentury office is the meeting point of these cultures, where the assembly line efficiency management of the factory meets the quantifying control of the knowledge economy. A Guide to Office Clerical Time Standards explores the survival of one regime into its successor following the lines of combined and uneven development that have turned the emancipatory promise of immaterial labour into the perma-temp hell of the cognitariat. The movement is accompanied by a score of guitar, bass and drums, the componenets of the rock ‘n’ roll music that rose from the car factories of the motor city and the cotton fields of the southern states to represent the same junction of expression and control.

Camera and performer: energetic engagement with the shield

The stylistic strategies, in particular those concerning camera placement and movement, of The Shield (FX, 2002-08) seem to directly fit into an aesthetic tradition developed by US cop dramas like Hill Street Blues (NBC, 1981-87), Homicide: Life on the Street (NBC, 1993-99) and NYPD Blue (ABC, 1993-2005). In these precinct dramas, decisions concerning spatial arrangements of camera and performer foreground a desire to present and react to action while it is happening, and with a minimum of apparent construction. As Jonathan Bignell (2009) has argued, the intimacy and immediacy of this stylistic approach, which has at its core an attempt at a documentary-like realism, is important to the police drama as a genre, while also being tendencies that have been taken as specific characteristics of television more generally. I explore how The Shield develops this tradition of a reactive camera style in its strategy of shooting with two cameras rather than one, with specific attention to how this shapes the presentation of performance. Through a detailed examination of the relationship between performer and camera(s) the chapter considers the way the series establishes access to the fictional world, which is crucial to the manner of police investigation central to its drama, and the impact of this on how we engage with performance. The cameras’ placement appears to balance various impulses, including: the demands of attending to an ensemble cast, spontaneous performance style, and action that is physically dynamic and involving. In a series that makes stylistic decisions around presentation of the body on-screen deliberately close yet obstructive, involving yet fleeting, the chapter explores the affect of this on the watching experience.

The effects of stocking density in physiological parameters and growth of the endangered teleost species piabanha, Brycon insignis (Steindachner, 1877)

This study investigated the effects of stocking density on the growth and fatty acid (FA) of Brycon insignis metabolism. Fingerlings (360) were distributed into eight ponds at two stocking densities (105 and 210 g/m(3)). The analysis of growth showed that the condition factor (K) and the coefficient of variation (CV) for body mass were not affected by stocking density. However, final body mass and length, specific growth rate (SGR), and weight gain (WG) were higher in the low stocking density group, which also presented a higher feed efficiency (FE) and survival (S). By contrast, muscle protein levels were higher in the high stocking density group. The plasma and muscle lipid content were not affected by stocking density, but fish reared at lower stocking density presented higher lipid concentration in the liver, with no differences in hepatosomatic index values. Even with the differences observed in metabolic and growth parameters, plasma cortisol was not affected by stocking density. The FA profile in the muscle and liver neutral fraction were not affected by stocking density, but the FA in the polar fractions differed between the two stocking densities. In the liver, total polyunsaturated fatty acids (PUFA) and PUFA n - 3 increased in higher stocking density, mainly due to an increase in docosahexaenoic acid (DHA). In addition, PUFA n - 6 were also increased in the higher stocking density group, mainly due to an increase in arachidonic acid (AA) and docosadienoic acid (22:2n - 6). In the muscle polar fraction, the saturated fatty acids (SFA) and monounsaturated fatty acids (MUFA) decreased in the animals from the higher stocking density group, and this reduction was compensated by an increase in PUFA n - 3 and PUFA n - 6, mainly the FA with 20-22 carbons (20:4n - 6: 22:4:n - 6; 22:5n - 6, 22:5n - 3, and 22:6n - 3). A different profile was observed for the C18 PUFAs, mainly 18:2n - 6 and 18:4n - 6, which were higher in the lower density stocking group. The data suggest that when living in high stocking density, B. insignis differentially utilizes the hepatic lipids as energy source and remodels the membrane fatty acids, with higher amounts of DHA in the polar muscle fraction compensated for by a decrease in MUFA. The zootechnical and physiological indices reveal that the lower stocking density group achieve overall better performance. (C) 2010 Elsevier B.V. All rights reserved.

Complex rearrangements in patients with duplications of MECP2 can occur by fork stalling and template switching

Duplication at the Xq28 band including the MECP2 gene is one of the most common genomic rearrangements identified in neurodevelopmentally delayed males. Such duplications are non-recurrent and can be generated by a non-homologous end joining (NHEJ) mechanism. We investigated the potential mechanisms for MECP2 duplication and examined whether genomic architectural features may play a role in their origin using a custom designed 4-Mb tiling-path oligonucleotide array CGH assay. Each of the 30 patients analyzed showed a unique duplication varying in size from similar to 250 kb to similar to 2.6 Mb. Interestingly, in 77% of these non-recurrent duplications, the distal breakpoints grouped within a 215 kb genomic interval, located 47 kb telomeric to the MECP2 gene. The genomic architecture of this region contains both direct and inverted low-copy repeat (LCR) sequences; this same region undergoes polymorphic structural variation in the general population. Array CGH revealed complex rearrangements in eight patients; in six patients the duplication contained an embedded triplicated segment, and in the other two, stretches of non-duplicated sequences occurred within the duplicated region. Breakpoint junction sequencing was achieved in four duplications and identified an inversion in one patient, demonstrating further complexity. We propose that the presence of LCRs in the vicinity of the MECP2 gene may generate an unstable DNA structure that can induce DNA strand lesions, such as a collapsed fork, and facilitate a Fork Stalling and Template Switching event producing the complex rearrangements involving MECP2.

Fatty acids as trophic biomarkers in vitellogenic females in an impounded tropical river

Fatty acids have been used in marine biogeochemistry as food chain biomarkers, but in freshwater these studies are rare. In order to evaluate the fatty acid potential as biomarkers in freshwater, their profile was analyzed during vitellogenesis in two fish species, in both waterfall and reservoir environments of the Paraiba do Sul River Basin. Detrivorous Hypostomus affinis and omnivorous Geophagus brasiliensis seem to elongate and desaturate polyunsaturated fatty acids (PUFA) and transfer them to the ovaries` phospholipids. Waterfall Geophagus brasiliensis have more highly unsaturated fatty acids in the liver, but in the reservoir, accumulation mainly occurs in muscle and ovary triglycerides, suggesting trophic opportunism and a plasticity during vitellogenesis. In Hypostomus affinis, PUFA alteration occurs only in the reservoir, suggesting a high phytoplankton occurrence. Eutrophication and water speed is reflected in Hypostomus affinis ovaries by higher PUFAn3 and bacterial fatty acids. As in marine environments, analysis of mono- and polyunsaturated fatty acids during vitellogenesis can be used as a tool in food chain studies in freshwater.

Genetic and Functional Evidence Implicating DLL1 as the Gene That Influences Susceptibility to Visceral Leishmaniasis at Chromosome 6q27

Background. Visceral leishmaniasis (VL) is caused by Leishmania donovani and Leishmania infantum chagasi. Genome-wide linkage studies from Sudan and Brazil identified a putative susceptibility locus on chromosome 6q27. Methods. Twenty-two single-nucleotide polymorphisms (SNPs) at genes PHF10, C6orf70, DLL1, FAM120B, PSMB1, and TBP were genotyped in 193 VL cases from 85 Sudanese families, and 8 SNPs at genes PHF10, C6orf70, DLL1, PSMB1, and TBP were genotyped in 194 VL cases from 80 Brazilian families. Family-based association, haplotype, and linkage disequilibrium analyses were performed. Multispecies comparative sequence analysis was used to identify conserved noncoding sequences carrying putative regulatory elements. Quantitative reverse-transcription polymerase chain reaction measured expression of candidate genes in splenic aspirates from Indian patients with VL compared with that in the control spleen sample. Results. Positive associations were observed at PHF10, C6orf70, DLL1, PSMB1, and TBP in Sudan, but only at DLL1 in Brazil (combined P = 3 x 10(-4) at DLL1 across Sudan and Brazil). No functional coding region variants were observed in resequencing of 22 Sudanese VL cases. DLL1 expression was significantly (P = 2 x 10(-7)) reduced (mean fold change, 3.5 [SEM, 0.7]) in splenic aspirates from patients with VL, whereas other 6q27 genes showed higher levels (1.27 x 10(-6) < P < .01) than did the control spleen sample. A cluster of conserved noncoding sequences with putative regulatory variants was identified in the distal promoter of DLL1. Conclusions. DLL1, which encodes Delta-like 1, the ligand for Notch3, is strongly implicated as the chromosome 6q27 VL susceptibility gene.

Liderança em Resorts: um estudo dos estilos praticados no Brasil

GIULIANI, Thais de Almeida. Liderança em Resorts: um estudo dos estilos praticados no Brasil. 2012. 136p. Dissertação (Mestrado em Administração)- Universidade Municipal de São Caetano do Sul, São Caetano do Sul, 2012.

De trabalhadores produzidos a sujeitos de uma práxis educativa: estudo e proposta de ação na região do Vale do Rio Preto

o Vale do Rio Preto, região limítrofe dos Estados do Rio de Janeiro e Minas Gerais, teve marcante influência na economia e na política do país no século XIX, com a expansão da cafeicultura. A partir de 1930, com a crise do café, a paisagem agrária é dominada pela pecuária extensiva. A transformação do sistema econômico modifica as atividades produtivas, embora perpetue as tradicionais relações de produção. A educação desenvolvida neste vale, nas duas últimas décadas, fundamenta-se na teoria do capital humano que enfatiza a racionalidade e produtividade, impedindo o desenvolvimento da consciência crítica. A redução da educação a essa concepção desenvolve mentalidades ou consciências alienadas das verdadeiras determinações sociais, a concentração de capital, as desigualdades e injustiças. Para que os trabalhadores da educação, produzidos em tais circunstâncias de dominação, venham a assumir o papel de sujeitos hegemônicos na construção da sociedade, é indispensável que se apropriem de instrumentos práticos e teóricos, embasados numa fundamentação que reverta esse quadro social. Para tal, apontamos como uma proposta de devir, a adesão dos profissionais da educação à perspectiva da praxis, para a realização de um trabalho crítico e consciente com os pequenos produtores rurais. A tarefa de socialização do conhecimento, a trans formação da visão de mundo e da prática educativa dos profissionais que se propõem a desenvolver uma açao conjunta com os pequenos produtores do Vale do Rio Preto, só poderão ocorrer a partir de inovações das relações no real.

Os estilos de liderança dos líderes escolares da Região Autónoma da Madeira

A liderança é um conceito que tem sido alvo de várias interpretações e definições no último século. O líder, em geral, tem sido visto como alguém que possui determinadas características inatas ou adquiridas, alguém que se adapta às circunstâncias e ao contexto em que a organização se insere e alguém que gere conflitos e exerce influência em ambientes ambíguos, complexos e incertos. O professor enquanto gestor/líder escolar pode assumir diferentes estilos/comportamentos na liderança da sua organização escolar: transformacional, transaccional e “laisser-faire”. Os principais «constructos» de liderança – transformacional, transaccional e passiva – formam um novo paradigma, teoria full range (Brass e Avolio, 2004) para a compreensão dos efeitos do estilo de liderança. A análise do impacto que alguns indivíduos têm sobre as suas organizações tem despertado um interesse crescente. Esses indivíduos podem ser chamados líderes carismáticos (Weber, 1968) ou transformacionais (Bass, 1985; 1990) que são líderes que, através de sua visão pessoal e de sua energia, inspiram os seguidores e têm um impacto significativo em suas organizações. Nas suas investigações sobre o conceito de liderança, Bernard Bass (1985) e Avolio (1999) comparam dois tipos de comportamento de liderança: transaccional e transformacional. Os líderes transaccionais determinam o que os subordinados precisam para realizar seus próprios objectivos e os objectivos da organização. Em contraste, os líderes transformacionais “motivam-nos a fazer mais do que originalmente esperávamos realizar” (Bass 1985, p. 28) elevando nosso sentimento da importância e do valor de nossas tarefas, “fazendo-nos transcender nossos interesses pessoais em nome da equipa, da organização ou de uma política mais ampla” (Bass,1985, p. 29) e elevando nosso nível de necessidade para as necessidades mais altas,como a auto-realização. Este estudo de investigação tem por fim determinar o modo como os docentes percepcionam a liderança das suas organizações escolares: transformacional, transaccional ou “laisser-faire”. Foi utilizado como instrumento de recolha de dados o Multifactor Leadership Questionnaire (MLQ) desenvolvido por Bass e Avolio (2004) o qual determina/identifica os estilos de liderança através da avaliação dos comportamentos do líder percepcionados pelos seus liderados. O MLQ foi aplicado a uma amostra de 97 escolas na Região Autónoma da Madeira nos meses de Janeiro, Fevereiro e Março de 2008.

Estilo de liderança da líder do conselho executivo numa escola básica dos 2º e 3º ciclos pública da Região Autónoma da Madeira

O conceito de liderança é um tema que sempre despertou interesse, desde as primeiras civilizações até aos nossos dias. No entanto, só no século passado é que começou a ser mais desenvolvido e estudado pelos especialistas em comportamentos organizacionais. Como vivemos numa época marcada pela globalização e pela mundialização dos problemas, onde a tecnologia e o conhecimento experimentam um grande desenvolvimento, de dia para dia, as lideranças são chamadas a acompanhar todas estas mudanças. É nestas circunstâncias que os líderes desempenham um papel de destaque nas organizações, onde têm de tomar decisões estratégicas e, ao mesmo tempo, ter a capacidade de motivar todos os agentes que colaboram com a organização. Nesta perspectiva, realizámos o trabalho de investigação empírica que aqui apresentamos, com o propósito de verificar qual o estilo de liderança da líder de uma Escola Básica dos 2.º e 3.º ciclos do ensino público da Região Autónoma da Madeira. Também pretendemos verificar se existem na instituição outras lideranças e que relações estabelecem com a liderança formal. Na recolha dos dados, utilizámos uma abordagem qualitativa e quantitativa, através de um estudo de caso, com recurso às técnicas de entrevista e de inquéritos por questionário. Nesta última técnica, optámos pela utilização do Multifactor Leadership Questionnaire (Questionário Multifactorial de Liderança), já testado e desenvolvido por Bass e Avolio (2004), para avaliar os comportamentos da líder através das percepções dos liderados. Apesar das limitações de um trabalho desta natureza, conseguimos, através das técnicas de investigação utilizadas, verificar qual o estilo de liderança em que a líder da instituição se enquadra. Verificámos ainda que os restantes membros que compõem a direcção executiva não partilham do mesmo estilo de liderança da líder e que na instituição existem outras lideranças informais, ao nível do corpo docente, que ora colaboram, ora fazem oposição à liderança formal.