Control of oocyte maturation

Oocyte maturation is a complex process involving nuclear and cytoplasmic maturation. The nuclear maturation is a chromosomal segregation and the cytoplasmic maturation involves the reorganization of the cytoplasmic organelles, mRNA transcription and storage of proteins to be used during fertilization and early embryo development. The mechanism of oocyte maturation in vivo and in vitro still are not totally understood. However it is generally accepted that the second messenger cyclic adenosine monophosphate (cAMP) plays a critical role in the maintenance of meiotic blockage of mammalian oocytes. A relative increase in the level of cAMP within the oocyte is essential for maintaining meiosis block, while a decrease in cAMP oocyte concentration allows the resumption of meiosis. The oocyte cAMP concentration is regulated by a balance of two types of enzymes: adenylate cyclase (AC) and phosphodiesterases (PDEs), which are responsible for the synthesis and degradation of cAMP, respectively. After being synthesized by AC in cumulus cells, cAMP are transferred to the oocyte through gap junctions. Thus, specific subtypes PDEs are able to inhibit or attenuate the spontaneous meiotic maturation of oocytes with PDE4 primarily involved in the metabolism of cAMP in granulosa cells and PDE3 in the oocyte. Although the immature oocytes can resume meiosis in vitro, after being removed from antral follicles, cytoplasmic maturation seems to occur asynchronously with nuclear maturation. Therefore, knowledge of the oocyte maturation process is fundamental for the development of methodologies to increase the success of in vitro embryo production and to develop treatments for various forms of infertility. This review will present current knowledge about the maintenance of the oocyte in prophase arrest, and the resumption of meiosis during oocyte maturation, focusing mainly on the changes that take place in the oocyte.

Zoneamento geoambiental como instrumento de planejamento e gestão de recursos do pólo cerâmico de Santa Gertrudes

The disorded and unnplaned occupation is not recent in out territory but it concerns to a historical territorial invasion that roots the time of our country discovery. However, they have started to cause issues nowadays, as in the case of urban conurbations. Somehow these unorganized urban, industrial and agricultural occupations did not have any sort of information or indication of the appropriate situation at that time. Because of that there has been researches by several groups to induce the users of the several renewable and non renewable resources to explore them according to their natural potential capacity.The Ceramic Pole of Santa Gertrudes, the national largest producer of ceramic tile, faces this problem of disorder occupation. Among other themes, the reorganization of the physical environment, proposed by several groups of researchers and scholars of this subject, is gaining attention now.The method of Geoenvironmental Zoning results in a way of prevention from this issue. Crossing information of the physical environment and generating maps whose areas and subdivisions have similar characteristics, it is possible to manage studies and handle these areas according to their potentiality.

Regeneração natural em ecounidades maduras e em reorganização de fragmentos de Floresta Estacional Semidecídua

The Atlantic Rainforest biome has been going through fragmentation processes caused by agriculture and urbanization in green areas. Structural studies associated with the silvigenetic approach allow the understanding of what the past has caused in the present structure and predict future conditions of disturbed fragments. The objective of this study was to compare the composition and diversity of arboreous natural regeneration of steady-state and reorganization ecounits in two Seasonal Semideciduous Forest fragments. The hypothesis was that specific composition varies in these two different ecounits due to differential adaptation of species in canopy gaps and closed canopy. The survey was made in three areas with different perturbation backgrounds of 0,5 ha each. 60 permanent plots of 4m² each (2m x 2m) were stablished along the studied fragments following the proportion of ecounits presented in a previous mapping. Each plot was divided in 4 sub-plots of 1m² and arboreous individuals between 0,20m and 1,30m height were sampled and posteriorly separated in two height classes: I) individuals between 0,20m and 0,50m height (2m² sampling) and II) individuals between 0,51m and 1,30m height (4m² sampling). It was sampled 338 individuals from 53 families and 23 species. The Shannon index was 3,26 (Area A), 2,27 (Area B) and 2,42 (Area C) whereas Areas B and C values are considered low in our state Semidecidous Forests. Steady-state ecounits presented the highest values for abundance and species richness. Chi-square test pointed out species’ selection for determined ecounits in the studied community. Rarefaction method analysis showed diversity increase in steady-state ecounits and a stablishment in species richness curves for reorganization ecounits

Flutuação e gramaticalização no paradigma dos juntores em português forma, significado e história de (na) hora que

In this paper, I focus on one type of grammatical change consisting of creating periphrastic junctures from nominal sources used in relative clause contexts. This is a periphrase production mechanism that has been increasing the unstable paradigm of junctures and helping delineate Portuguese language grammar. One construction resulting from this mechanism is (na) hora que, in relation to which I propose to investigate form, meaning and history. The central aim is to show the emergence of (na) hora que, a grammaticalization instance in course, in which it is possible to catch stages of the its gradual constitution process, concerning both the syntagmatic reorganization of items with consequent morphological losses, and the constitution of meanings, with the origin of polysemies, pointing to a directionality grounded on the increase of the cognitive complexity

Morphological changes of mammalian nucleoli during spermatogenesis and their possible role in the chromatoid body assembling

Chromatoid body (CB) is a typical cytoplasmic organelle of germ cells, and it seems to be involved in RNA/protein accumulation for later germ-cell differentiation. Despite most of the events in mammals spermatogenesis had been widely described in the past decades and the increase in the studies related to the CB molecular composition and physiology, the origins and functions of this important structure of male germ cells are still unclear. The aims of this study were to describe the nucleolar cycle and also to find some relationship between the nucleolar organization and the CB assembling during the spermatogenesis in mammals. Cytochemical and cytogenetics analysis showed nucleolar fragmentation in post-pachytene spermatocytes and nucleolar reorganization in post-meiotic spermatids. Significant difference in the number and in the size of nucleoli between spermatogonia and round spermatids, as well as differences in the nucleolar position within the nucleus were also observed. Ultrastructural analysis showed the CB assembling in the cytoplasm of primary spermatocytes and the nucleolar fragmentation occurring at the same time. In conclusion our results suggest that the CB may play important roles during the spermatogenesis process in mammals and that its origin may be related to the nucleolar cycle during the meiotic cell cycle.

Key participants of the tumor microenvironment of the prostate: An approach of the structural dynamic of cellular elements and extracellular matrix components during epithelial-stromal transition

Cancer is a multistep process that begins with the transformation of normal epithelial cells and continues with tumor growth, stromal invasion and metastasis. The remodeling of the peritumoral environment is decisive for the onset of tumor invasiveness. This event is dependent on epithelial–stromal interactions, degradation of extracellular matrix components and reorganization of fibrillar components. Our research group has studied in a new proposed rodent model the participation of cellular and molecular components in the prostate microenvironment that contributes to cancer progression. Our group adopted the gerbil Meriones unguiculatus as an alternative experimental model for prostate cancer study. This model has presented significant responses to hormonal treatments and to development of spontaneous and induced neoplasias. The data obtained indicate reorganization of type I collagen fibers and reticular fibers, synthesis of new components such as tenascin and proteoglycans, degradation of basement membrane components and elastic fibers and increased expression of metalloproteinases. Fibroblasts that border the region, apparently participate in the stromal reaction. The roles of each of these events, as well as some signaling molecules, participants of neoplastic progression and factors that promote genetic reprogramming during epithelial–stromal transition are also discussed.

Atividades investigativas no ensino da matemática financeira: as estratégias empregadas com uso de planilhas eletrônicas

Pós-graduação em Educação Matemática - IGCE

Políticas públicas em educação e o ideário neoliberal e pós-moderno: a ciência geográfica como instrumento crítico de análise da conjuntura contemporânea

Pós-graduação em Educação Escolar - FCLAR

Avaliação do conhecimento e do grau de satisfação dos usuários em relação à estratégia de saúde da família no município de Pedregulho-SP

Pós-graduação em Serviço Social - FCHS

Procedimentos de pesquisa em Geografia do Comércio e do Consumo: delimitação, intensidade e especialização de áreas centrais. Análises a partir de Marília/SP, São Carlos/SP e São José do Rio Preto/SP

This academic report presents results of the research developed between 2012 and 2015, and it had as main goal the development of new mapping possibilities of the central areas of the cities of Marília/SP, São Carlos/SP and São José do Rio Preto/SP. We developed this research using mostly the database from Cadastro Nacional de Endereços Para Fins Estatísticos (CNEFE), and after processing the information, it was added the typology established in the Cadastro Nacional de Atividades Econômicas (CNAE). This database, after the final treatment process, provided us information that made possible the production of thematic maps which are used to study central areas, allowing the analysis of spatial logics of companies, the identification of concentration and dispersion of economic activities, arrangement of specialized axis, expansion of the central area, reorganization of the city, among other process and phenomena

Teoria crítica, neoliberalismo e educação: análise reflexiva da realidade educacional brasileira a partir da reforma educacional de 1990

Pós-graduação em Educação - FFC

Infância e escola: desafios no ingresso da criança no ensino fundamental

In order to expand educational services in the country, were recently approved laws that changed the organization of schools and the population served by them, as was the case of Law No. 11.274/2006 elementary school that extended from eight to nine years, establishing the admission of children to this level of education to 6 years old. The research aimed to analyze the relationship between the legal text and the meanings of schooling and the specifics of childhood with the entry of children in elementary school. We proceeded to carry out theoretical research and documentation and with the main results we can state that: 1) referring to the childhood and school education, we can reflect on the period that includes the ages between 5 and 7 years, periods in which the child Brazil, according to new legislation can be met in kindergarten and compulsory from 6 years in elementary education, refers to the student at a time conducive to the psychological and cognitive investment in the development of imagination, thought through images, perception , logical reasoning, comparison and identification of elements of the property, creativity and playful and symbolic creations, 2) found that the formation of the 1st child. years of elementary school students is directed around the practices of literacy, with emphasis on the acquisition of reading and writing; Find out what this new educational context, schools are presenting great challenges due to the specifics of care for children six years in the first year Elementary School, showing gap between the law and the actual developments in the reorganization of schools.

Alexandre Herculano e a voz do profeta

The paper discusses briefly the emergence of Alexandre Herculano, intellectual committed to its own historical time and engaged in the process of reorganization of the Portuguese culture through his journalistic activity, in Portuguese literary scene through the publication of The prophet’s voice.

Configurando um AVO (Ambiente Virtual Anise de Abreu Gonçalves D'Orange Ferreira de Orientação): instrumentação de um professor em proposta de incentivo ao uso de TIC e AVA no nível de pós-graduação

In this paper, I will present a proposal for teachers’instrumentation in ICT by the configuration of a virtual learningenvironment (MOODLE) for advising Linguistics graduate students’projects on teacher education and language teaching mediated by digitaltechnologies. The context of the proposal is teaching as work,occasioned by an announcement from the IHE to encourage the use ofICT. Following the theoretical assumptions adopted by the ALTERresearch group, and confronted with the goals set in the text of theannouncement, the instrumentation involved the reorganization ofcontents provided by a graduate course in the research process steps:planning, implementation and dissemination, and of the artifactsaffordances: information, communication and production.

Micro-arc oxidation as a tool to develop multifunctional calcium-rich surfaces for dental implant applications

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)