Sérgio e Verney: O humanismo científico e a reforma de mentalidades

Analisam-se as referências a Verney na obra de António Sérgio, inscrevendo-as na sua interpretação da história de Portugal, com incidência na tópica do seiscentismo e da decadência portuguesa, face à Grande Revolução Científica que culmina em Newton.

Legato: um modelo de avaliação ilustrativo da construção do conhecimento científico

Na unidade curricular de Biologia do Desenvolvimento, lecionada desde 2011 ao 2º ano da licenciatura de Biologia Humana na Universidade de Évora, tem-se aplicado um modelo de avaliação que visa focalizar a aprendizagem através da construção de trabalhos-projeto que são realizados, em duas fases, por dois grupos de alunos. Este modelo de avaliação foi motivado tanto pelo facto do conteúdo desta unidade curricular ser extremamente complexo para uma avaliação mais convencional, como pela oportunidade que dá aos alunos de se aproximarem do contexto profissional de construção do conhecimento científico por interação entre pares. Na primeira fase de avaliação, o tema e a composição do grupo são escolhidos pelos alunos, sendo completada a pouco mais de meio do semestre com a submissão dum resumo (revisão) da informação coligida, bibliografia, e uma breve apresentação para os colegas, que deste modo ficam com uma panorâmica das temáticas a desenvolver no respetivo ano letivo. Na segunda fase essa informação é legada a um novo grupo, que desta vez é sorteado entre a turma, e que deverá seguir um guião preparado pelo docente, com base no trabalho previamente apresentado, para preparar uma apresentação final, em sessão pública. Cada tema tem por isso dois grupos de alunos diretamente envolvidos, que se interligam num elo de responsabilidade mútua que irá desembocar na sessão pública final. O nome proposto para este modelo, Legato, realça esta interligação. As apresentações finais, depois de eventuais correções, ficam disponíveis publicamente na World Wide Web. O papel das aulas é ir introduzindo os alunos à disciplina científica de Biologia do Desenvolvimento, facultando-lhes os conceitos, a linguagem, e sobretudo o raciocínio que lhe são próprios. Em duas sessões práticas apresenta-se aos alunos o mundo das bases de dados biológicas e bibliográficas (respetivamente), para que consigam um levantamento mais eficiente de fontes relevantes. Decorridos 5 anos desta prática de avaliação, com mais de 30 destes trabalhosprojeto já realizados, tem-se verificado um aproveitamento muito satisfatório na generalidade dos temas, mesmo com aqueles (poucos) que sofreram da falta de empenhamento do grupo da 1ª fase. O caráter público da fase final de avaliação, de par com a preparação (por parte do docente) do guião da 2ª fase, contribuem para garantir a qualidade das apresentações finais. Mas o autor acredita que a principal motivação para a qualidade generalizada dos resultados é o elo de responsabilidade mútua referido acima, bem como uma sensação de “pertença” em relação aos temas escolhidos. Este modelo de avaliação pode ser adaptado em qualquer área do conhecimento, pois a construção do conhecimento entre pares, que se pretende ilustrar neste modelo de avaliação, é essencialmente universal; e também em diferentes fases da formação, se bem que seja especialmente adequado a unidades curriculares do 1º Ciclo do Ensino Superior.

La investigación científico-social: Un debate epistemológico desde la educación

Recibido 27 de julio de 2011 • Aceptado 26 de agosto de 2011 • Corregido 28 de octubre de 2011  El debate epistemológico de la investigación científico-social durante años ha consistido en confrontar los métodos, tratando de hacer valer más uno que el otro a partir de la gran teoría que lo soporta. Aunque propuestas más recientes recuperan métodos híbridos o planteamientos epistemológicos más diversos, la postura de la dualidad ha prevalecido. Descriptiva o explicativa; fenomenológica o positivista; clásica o emergente; los paradigmas de investigación son la manera en cómo tenemos acceso a la realidad investigada y así obtener principios y respuestas. Ello define los métodos. Aún cuando es posible agrupar y categorizar estos métodos en cuantitativos y cualitativos, el entramado epistemológico que establecen las estructuras de cualquier metodología permitirá el abordaje de un fenómeno mejor que otro, sin que esto pondere necesariamente alguno de manera definitoria. Dentro de la educación, tanto métodos cuantitativos como cualitativos han ayudado a abordar fenómenos de tal manera que han producido resultados favorables para el bienestar del quehacer educativo. Este ensayo, producto de una revisión bibliográfica y una trayectoria investigativa y educativa, pretende esclarecer el complejo panorama inicial de los investigadores educativos novatos antes de revisar y recuperar una postura epistemológica.

Elementos género sensitivos para mirar la participación de hombres y mujeres en la construcción del conocimiento científico

La propuesta que se desarrolla a continuación, es una mirada desde la teoría y el aporte de la perspectiva de género para el análisis de algunos elementos que juegan un papel importante en la participación de hombres y mujeres en el ámbito de la ciencia. Esta propuesta no implica un marco fijo para el abordaje de la temática, sino que presenta fundamentos que pueden ayudar a comprender por qué, a pesar de que los índices de participación educativa en colegios y universidades son más altos en el caso de las mujeres que en el de los hombres, las mujeres no se integran en paridad en el hacer científico. Este artículo forma parte del informe final del proyecto de investigación UNA-Equidad, de la Escuela de Sociología, de la Universidad Nacional.

Metodologia científica: do problema à elaboração do trabalho científico

Material utilizado no módulo de Metodologia Científica do curso de especialização em Nefrologia produzido pela UNA-SUS/UFMA. Esta unidade apresenta subsídios teóricos para a produção do Projeto de Intervenção, plano de ação que visa a elaboração de um planejamento estratégico situacional a partir do contexto de trabalho, amplamente utilizado na área da saúde.

Analysis Of Cellular Adhesion On Superhydrophobic And Superhydrophilic Vertically Aligned Carbon Nanotube Scaffolds.

We analyzed GFP cells after 24h cultivated on superhydrophilic vertically aligned carbon nanotube scaffolds. We produced two different densities of VACNT scaffolds on Ti using Ni or Fe catalysts. A simple and fast oxygen plasma treatment promoted the superhydrophilicity of them. We used five different substrates, such as: as-grown VACNT produced using Ni as catalyst (Ni), as-grown VACNT produced using Fe as catalyst (Fe), VACNT-O produced using Ni as catalyst (NiO), VACNT-O produced using Fe as catalyst (FeO) and Ti (control). The 4',6-diamidino-2-phenylindole reagent nuclei stained the adherent cells cultivated on five different analyzed scaffolds. We used fluorescence microscopy for image collect, ImageJ® to count adhered cell and GraphPad Prism 5® for statistical analysis. We demonstrated in crescent order: Fe, Ni, NiO, FeO and Ti scaffolds that had an improved cellular adhesion. Oxygen treatment associated to high VACNT density (group FeO) presented significantly superior cell adhesion up to 24h. However, they do not show significant differences compared with Ti substrates (control). We demonstrated that all the analyzed substrates were nontoxic. Also, we proposed that the density and hydrophilicity influenced the cell adhesion behavior.

Characterization Of Cumulative Joint Damage Patterns In Patients With Rheumatoid Arthritis: A Clinical, Serological, And Gene Polymorphism Perspective.

To characterize cumulative joint damage (CJD) patterns in rheumatoid arthritis (RA) and determine their associations with demographic/clinical features and HLA-DRB1 gene polymorphism. Hand and foot radiographs were obtained from 404 patients with RA. CJD patterns were determined by 3 derivations from Sharp/van der Heijde scores, obtained by the mathematical division of scores for hands/feet (Sharp-h/f score), fingers/wrists (Sharp-f/w score), and erosion/space narrowing (Sharp-e/sn score), respectively. DNA and serum were obtained for determination of HLA-DRB1 polymorphism, rheumatoid factor (RF), and anticitrullinated protein antibodies (ACPA). Patients with wrist-dominant CJD pattern were more likely to have severe RA than those with finger-dominant pattern (68.4% vs 46.0%; p = 0.036) as were those with foot-dominant vs hand-dominant CJD pattern (76.5% vs 56.4%; p = 0.044). HLA-DRB1 shared epitope (SE) alleles were associated with erosion-dominant CJD pattern (p = 0.021). Patients with erosion-dominant CJD pattern had higher levels of RF and ACPA than those with space-narrowing-dominant CJD pattern (median RF 71.35 U/ml vs 22.05 U/ml, respectively; p = 0.003; median ACPA 187.9 U/ml vs 143.2 U/ml, respectively; p < 0.001). The majority of triple-positive patients (SE+, RF+, ACPA+) had erosion-dominant CJD pattern (62.3%) while the majority of triple-negative patients (SE-, FR-, ACPA-) had space narrowing-dominant CJD pattern (75%; p = 0.017). ACPA was associated with HLA-DRB1 SE alleles (p < 0.05). Patients with foot-dominant CJD pattern were taller than those with hand-dominant CJD pattern (p = 0.002); those with erosion-dominant CJD pattern had higher weight and body mass index than those with space narrowing-dominant CJD pattern (p = 0.014, p = 0.001). CJD patterns were associated with disease severity, HLA-DRB1 SE status, presence and titer of ACPA and RF, and morphometric features.

Intercâmbios acadêmicos internacionais: bolsas Capes, CNPq e Fapesp

This article is part of a study considering the growing importance of the international transit of people, knowledge, and practices in the schooling and professional education processes of some social segments. Considering the public funds made available by the Coordination for the Improvement of Higher Education Personnel - Capes -, the National Council for Scientific and Technological Development - CNPq - and the State of São Paulo Research Foundation - Fapesp - to support researchers' fellowships abroad, aming to improve research and investments on Science and Technology on the context of international exchanges, we have dedicated this article to the preliminary description and analysis of the database of fellows funded abroad by these research agencies from 1970 to 2000. The movement of flows based on the quantitative methodology of the correlation of variables draws the trends of international academic exchange programs in the three research institutions and in the different areas of knowledge, and we intend to analyse them taking into account the scientific and technological development policies adopted by Brazilian State on the period.

PARAFAC: uma ferramenta quimiométrica para tratamento de dados multidimensionais. Aplicações na determinação direta de fármacos em plasma humano por espectrofluorimetria

Since the last decade, the combined use of chemometrics and molecular spectroscopic techniques has become a new alternative for direct drug determination, without the need of physical separation. Among the new methodologies developed, the application of PARAFAC in the decomposition of spectrofluorimetric data should be highlighted. The first objective of this article is to describe the theoretical basis of PARAFAC. For this purpose, a discussion about the order of chemometric methods used in multivariate calibration and the development of multi-dimensional methods is presented first. The other objective of this article is to divulge for the Brazilian chemical community the potential of the combination PARAFAC/spectrofluorimetry for the determination of drugs in complex biological matrices. For this purpose, two applications aiming at determining, respectively, doxorrubicine and salicylate in human plasma are presented.

Quantificação de fluxos de mercúrio gasoso na interface solo/atmosfera utilizando câmara de fluxo dinâmica: aplicação na bacia do Rio Negro

Gaseous mercury sampling conditions were optimized and a dynamic flux chamber was used to measure the air/surface exchange of mercury in some areas of the Negro river basin with different vegetal coverings. At the two forest sites (flooding and non-flooding), low mercury fluxes were observed: maximum of 3 pmol m-2 h-1 - day and minimum of -1 pmol m-2 h-1 - night. At the deforested site, the mercury fluxes were higher and always positive: maximum of 26 pmol m-2 h-1 - day and 17 pmol m-2 h-1 - night. Our results showed that deforestation could be responsible for significantly increasing soil Hg emissions, mainly because of the high soil temperatures reached at deforested sites.

Óxido nítrico: propriedades e potenciais usos terapêuticos

Nitric oxide ( NO) is a substance that acts as a second-messenger and is associated with a number of important physiological functions such as regulation of the vascular tonus, immune modulation and neurotransmission. As a physiological mediator, alteration of its concentration level may cause pathophysiological disfunctions such as hypertension, septic shock and impotence. Possible therapeutic approaches are being developed to control NO levels in vivo. We review herein the main physical and chemical properties of NO, its biological functions and available chemical interventions to reduce and increment its physiological concentration levels. Recent developments in the field are also highlighted.

Polímeros biomiméticos em química analítica. Parte 1: preparo e aplicações de MIP (Molecularly Imprinted Polymers) em técnicas de extração e separação

MIPs are synthetic polymers that are used as biomimetic materials simulating the mechanism verified in natural entities such as antibodies and enzymes. Although MIPs have been successfully used as an outstanding tool for enhancing the selectivity or different analytical approaches, such as separation science and electrochemical and optical sensors, several parameters must be optimized during their synthesis. Therefore, the state-of-the-art of MIP production as well as the different polymerization methods are discussed. The potential selectivity of MIPs in the extraction and separation techniques focusing mainly on environmental, clinical and pharmaceutical samples as applications for analytical purposes is presented.

Polímeros biomiméticos em química analítica. Parte 2: aplicações de MIP (Molecularly Imprinted Polymers) no desenvolvimento de sensores químicos

The aim of this paper is the description of the strategies and advances in the use of MIP in the development of chemical sensors. MIP has been considered an emerging technology, which allows the synthesis of materials that can mimic some highly specific natural receptors such as antibodies and enzymes. In recent years a great number of publications have demonstrated a growth in their use as sensing phases in the construction of sensors . Thus, the MIP technology became very attractive as a promising analytical tool for the development of sensors.

Determinação direta de molibdênio em comprimidos polivitamínicos por voltametria em meio não aquoso

Adsorptive stripping voltammetry carried out in a homogeneous ternary solvent composed of N,N-dimethylformamide, water and ethanol, with alpha-benzoinoxime (alphaBO) as the complexing agent for Mo(VI) and a 0.5 mol L-1 acetic acid - sodium acetate buffer as supporting electrolyte was successfully used for the determination of molybdenum in polyvitamin-polymineral tablets. Tablet samples were analyzed and the results were compared with those obtained both by graphite furnace atomic absorption and by recovery tests, with good correlations, indicating that this may be considered as an alternative procedure for routine determination of Mo(VI) in pharmaceutical samples.

Cálculos teóricos de afinidades por próton de n-alquilaminas usando o método ONIOM

The ONIOM method was used to calculate the proton affinities (PA) of n-alkylamines (CnH2n+1NH2, n = 3 to 6, 8, 10, 12, 14, 16 and 18). The calculations were carried out at several levels (HF, MP2, B3LYP, QCISD(T), ...) using Pople basis sets and at the QCISD(T) level using basis sets developed by the generator coordinate method (GCM) and adapted to effective core potentials. PAs were also obtained through the GCM and high level methods, like ONIOM[QCISD(T)/6-31+G(2df,p):MP2/6-31G+G(d,p))//ONIOM[MP2/6-31+G(d,p):HF/6-31G]. The average error using the GCM, with respect to experimental data, was 3.4 kJ mol-1.