Questions in Narratives from Oral Tradition to Literature

Thesis (Ph.D.)--University of Washington, 2016-06

Changes to porcine blastocyst vitrification methods and improved litter size after transfer

The objective was to improve the protocol that was used to obtain the first reported piglets from transferred vitrified and warmed zona-intact blastocysts. Blastocysts were collected from superovulated sows and gilts, centrifuged to polarize lipid, vitrified, warmed and cultured for 24 h or transferred immediately. Removing the zona pellucida after warming increased the number of cells in the surviving blastocysts (zona-free 60.8 +/- 4.3, zona-intact 39.1 +/- 2.8; P < 0.05). Thinning the zona pellucida produced similar results to zona removal. Changing the basal medium of the vitrification and warming solutions from modified PBS to phosphate buffered NCSU-23 increased the number of cells (44.7 +/- 2.2 versus 56.0 +/- 3.9, respectively; P < 0.05). Reducing the plunge temperature of the liquid nitrogen from - 196 degrees C to less than -204 degrees C improved the embryo survival rate (61.9% versus 82.9%, respectively; P < 0.05). These modifications were incorporated into the vitrification protocol that was used to vitrify and warm 105 blastocysts (that were subsequently transferred into four recipients). Three recipients became pregnant, farrowing three litters (average litter size, 5.3; 18.8% embryo survival in farrowing sows). Changing the warming protocol to using sucrose rather than ethylene glycol resulted in a trend towards improved embryo survival (73.5% versus 91.2%) but this was not statistically significant. Incorporating this modification, 203 blastocysts were vitrified, warmed and transferred into seven recipients. Five became pregnant and 36 fetuses were recovered (average litter size 7.2; 24.8% embryo survival in pregnant sows) at Day 40 of pregnancy. In conclusion, changes made to the vitrification protocol improved pregnancy rate and in vivo embryo survival compared to an earlier study using the original protocol. (c) 2005 Elsevier Inc. All rights reserved.

Why children and adults sometimes (but not always) compute implicatures

Noveck (2001) argued that children even as old as 11 do not reliably endorse a scalar interpretation of weak scalar terms (some, might, or) (cf. Braine & Rumain, 1981; Smith, 1980). More recent studies suggest, however, that children's apparent failures may depend on the experimental demands (Papafragou & Musolino, 2003). Although previous studies involved children of different ages as well as different tasks, and are thus not directly comparable, nevertheless a common finding is that children do not seem to derive scalar implicatures to the same extent as adults do. The present article describes a series of experiments that were conducted with Italian speaking subjects (children and adults), focusing mainly on the scalar term some. Our goal was to carefully examine the specific conditions that allow the computation of implicatures by children. In so doing, we demonstrate that children as young as 7 (the youngest age of the children who participated in the Noveck study) are able to compute implicatures in experimental conditions that properly satisfy certain contextual prerequisites for deriving such implicatures. We also present further results that have general consequences for the research methodology employed in this area of study. Our research indicates that certain tasks mask children's understanding of scalar terms, not only including the task used by Noveck, but also tasks that employ certain explicit instructions, such as the training task used by Papafragou & Musolino (2003). Our findings indicate further that, although explicit training apparently improves children's ability to draw implicatures, children nevertheless fail to achieve adult levels of performance for most scalar terms even in such tasks, and that the effects of instruction do not last beyond the training session itself for most children. Another relevant finding of the present study is that some of the manipulations of the experimental context have an effect on all subjects, whereas others produce effects on just a subset of children. Individual differences of this kind may have been concealed in previous research because performance by individual subjects was not reported. Our general conclusions are that even young children (7-year olds) have the prerequisites for deriving scalar implicatures, although these abilities are revealed only when the conversational background is natural.

O PREGADOR SILENCIOSO: Ecumenismo no jornal Expositor Cristão (1886-1982)

O jornal Expositor Cristão, órgão oficial da Igreja Metodista no Brasil, nasceu em 1886, com uma proposta que se poderia chamar de ecumênica: seu primeiro nome, Methodista Catholico, revela o desejo de universalidade, confirmado pelo editorial de estréia. Seu criador, o missionário norte-americano John James Ransom, pretendia um veículo de orientação doutrinária que não fosse sectário, em consonância com a tradição wesleyana. Mas o Metodista Católico teve vida curta: em pouco mais de um ano passou a se chamar Expositor Cristão. O campo religioso brasileiro vivia, então, o período da controvérsia apologética, com a inserção do protestantismo de missão, de origem anglo-saxã, em contraposição ao catolicismo romano. O nome do recém-nascido jornal foi mais uma vítima dos embates. Este trabalho se dispôs a avaliar como o jornal tratou a questão do ecumenismo desde esse período até a entrada da Igreja Metodista no CONIC, Conselho Nacional de Igrejas Cristãs, no início da década de 1980. A pesquisa constatou que o anticatolicismo arraigado no metodismo brasileiro em seus primórdios jamais desapareceu por completo. Notou também que o metodismo não esteve imune aos conflitos interdenominacionais existentes no próprio meio evangélico. Em que pese o reconhecido pioneirismo da Igreja Metodista na criação de organismos ecumênicos brasileiros, o ecumenismo sempre enfrentou barreiras internas, mais ou menos explícitas. E o jornal Expositor Cristão, criado para ser um veículo de informação e formação doutrinária, nem sempre comunicou com a necessária clareza qual o significado que a Igreja Metodista confere à palavra ecumenismo e como ela o pratica. Em alguns momentos, a coexistência entre ecumenismo e antiecumenismo no interior do campo metodista não foi trazida à luz dos debates pelo jornal, mas permaneceu oculta por omissões e ambivalências. É o que este trabalho pôde constatar a partir de uma avaliação qualitativa do conteúdo do jornal.(AU)

A INTERNET NA EVOLUÇÃO DO PENSAMENTO FOLKCOMUNICACIONAL

Desde o início das pesquisas em folkcomunicação, essa área de estudo vem sofrendo uma evolução contínua com a descoberta de novos objetos de investigação diante de seu veloz desenvolvimento. A Internet está mudando a forma de como as pessoas se comunicam e vivem. No bojo dessas mudanças, a Folkcomunicação, que perpassa a comunicação das massas por ter sua gênese no folclore, vem ganhando novos objetos com a evolução das tecnologias da Informação. A questão principal que esse trabalho levanta é a de verificar como a Internet influenciou o pensamento da folkcomunicação. No entanto, este estudo pretende elucidar algumas perguntas, como: Quem são os novos líderes folkcomunicacionais? ; Como os meios de comunicação de massa influenciam o conteúdo da mensagem folk na Internet? ; A mensagem folk pela Internet teve de ser adaptada ao meio? ; Podemos encontrar manifestações para todas as audiências folk na Internet? , e Como as audiências recebem o conteúdo da comunicação? . Para tanto, buscou-se embasamento em conceitos de Luiz Beltrão, e atualizações deste em José Marques de Melo, Joseph Luyten, Cristina Schmidt, Osvaldo Meira Trigueiro, dentre outros. Os resultados obtidos levam ao entendimento de que as pesquisas do campo comunicacional no ambiente virtual na Internet seguem um padrão próximo das manifestações encontradas em outros suportes ainda que se possam verificar atualizações temporais e espaciais.(AU)

Knowledge representation with ontologies: present challenges future possibilities

Ontologies have become the knowledge representation medium of choice in recent years for a range of computer science specialities including the Semantic Web, Agents, and Bio-informatics. There has been a great deal of research and development in this area combined with hype and reaction. This special issue is concerned with the limitations of ontologies and how these can be addressed, together with a consideration of how we can circumvent or go beyond these constraints. The introduction places the discussion in context and presents the papers included in this issue.

The Internet and other IT resources:tools for translators within a translation programme

Information technology has increased both the speed and medium of communication between nations. It has brought the world closer, but it has also created new challenges for translation — how we think about it, how we carry it out and how we teach it. Translation and Information Technology has brought together experts in computational linguistics, machine translation, translation education, and translation studies to discuss how these new technologies work, the effect of electronic tools, such as the internet, bilingual corpora, and computer software, on translator education and the practice of translation, as well as the conceptual gaps raised by the interface of human and machine.

Die Sprache der Auricher Juden. Zur Rekonstruktion westjiddischer Sprachreste in Ostfriesland:to reconstruct westjiddischer language residues in Ostfriesland

Western Yiddish, the spoken language of the traditional Jewish society in the German- and Dutch-speaking countries, was abandoned by its speakers at the end of the 18th in favour of the emerging standard varieties: Dutch and German, respectively. Remnants of Western Yiddish varieties, however, remained a medium of discourse in remote provinces and could be found well into the 19th and sometimes the 20th century in some South-western areas of Germany and Switzerland, the Alsace, some areas of the Netherlands and in parts of the German province of Westphalia. It appears that rural Jewish communities sometimes preserved in-group vernaculars, which were based on Western Yiddish. Sources discovered in 2004 in the town of Aurich prove that Jews living in East Frisia, a Low-German speaking peninsula in the North-west of Germany, used a variety based on Western Yiddish until the Second World War. It appears that until the Holocaust a number of small, close-knit Jewish communities East Frisia, which depended economically mainly on cattle-trading and butchery, kept certain specific cultural features, among them the vernacular which they spoke alongside Low German and Standard German. The sources consist of two amateur theatre plays, a memoir and two word lists written in 1902, 1928 and the 1980s, respectively. In the monograph these sources are documented and annotated as well as analyzed linguistically against the background of rural Jewish life in Northern Germany. The study focuses on traces of language contact with Low German, processes of language change and on the question of the function of the variety in day-to-day life in a rural Jewish community.

The missing ingredient:inter-municipal cooperation and central-local relations in the UK

The thrust of the argument presented in this chapter is that inter-municipal cooperation (IMC) in the United Kingdom reflects local government's constitutional position and its exposure to the exigencies of Westminster (elected central government) and Whitehall (centre of the professional civil service that services central government). For the most part councils are without general powers of competence and are restricted in what they can do by Parliament. This suggests that the capacity for locally driven IMC is restricted and operates principally within a framework constructed by central government's policy objectives and legislation and the political expediencies of the governing political party. In practice, however, recent examples of IMC demonstrate that the practices are more complex than this initial analysis suggests. Central government may exert top-down pressures and impose hierarchical directives, but there are important countervailing forces. Constitutional changes in Scotland and Wales have shifted the locus of central- local relations away from Westminster and Whitehall. In England, the seeding of English government regional offices in 1994 has evolved into an important structural arrangement that encourages councils to work together. Within the local government community there is now widespread acknowledgement that to achieve the ambitious targets set by central government, councils are, by necessity, bound to cooperate and work with other agencies. In recent years, the fragmentation of public service delivery has affected the scope of IMC. Elected local government in the UK is now only one piece of a complex jigsaw of agencies that provides services to the public; whether it is with non-elected bodies, such as health authorities, public protection authorities (police and fire), voluntary nonprofit organisations or for-profit bodies, councils are expected to cooperate widely with agencies in their localities. Indeed, for projects such as regeneration and community renewal, councils may act as the coordinating agency but the success of such projects is measured by collaboration and partnership working (Davies 2002). To place these developments in context, IMC is an example of how, in spite of the fragmentation of traditional forms of government, councils work with other public service agencies and other councils through the medium of interagency partnerships, collaboration between organisations and a mixed economy of service providers. Such an analysis suggests that, following changes to the system of local government, contemporary forms of IMC are less dependent on vertical arrangements (top-down direction from central government) as they are replaced by horizontal modes (expansion of networks and partnership arrangements). Evidence suggests, however that central government continues to steer local authorities through the agency of inspectorates and regulatory bodies, and through policy initiatives, such as local strategic partnerships and local area agreements (Kelly 2006), thus questioning whether, in the case of UK local government, the shift from hierarchy to network and market solutions is less differentiated and transformation less complete than some literature suggests. Vertical or horizontal pressures may promote IMC, yet similar drivers may deter collaboration between local authorities. An example of negative vertical pressure was central government's change of the systems of local taxation during the 1980s. The new taxation regime replaced a tax on property with a tax on individual residency. Although the community charge lasted only a few years, it was a highpoint of the then Conservative government policy that encouraged councils to compete with each other on the basis of the level of local taxation. In practice, however, the complexity of local government funding in the UK rendered worthless any meaningful ambition of councils competing with each other, especially as central government granting to local authorities is predicated (however imperfectly) on at least notional equalisation between those areas with lower tax yields and the more prosperous locations. Horizontal pressures comprise factors such as planning decisions. Over the last quarter century, councils have competed on the granting of permission to out-of-town retail and leisure complexes, now recognised as detrimental to neighbouring authorities because economic forces prevail and local, independent shops are unable to compete with multiple companies. These examples illustrate tensions at the core of the UK polity of whether IMC is feasible when competition between local authorities heightened by local differences reduces opportunities for collaboration. An alternative perspective on IMC is to explore whether specific purposes or functions promote or restrict it. Whether in the principle areas of local government responsibilities relating to social welfare, development and maintenance of the local infrastructure or environmental matters, there are examples of IMC. But opportunities have diminished considerably as councils lost responsibility for services provision as a result of privatisation and transfer of powers to new government agencies or to central government. Over the last twenty years councils have lost their role in the provision of further-or higher-education, public transport and water/sewage. Councils have commissioning power but only a limited presence in providing housing needs, social care and waste management. In other words, as a result of central government policy, there are, in practice, currently far fewer opportunities for councils to cooperate. Since 1997, the New Labour government has promoted IMC through vertical drivers and the development; the operation of these policy initiatives is discussed following the framework of the editors. Current examples of IMC are notable for being driven by higher tiers of government, working with subordinate authorities in principal-agent relations. Collaboration between local authorities and intra-interand cross-sectoral partnerships are initiated by central government. In other words, IMC is shaped by hierarchical drivers from higher levels of government but, in practice, is locally varied and determined less by formula than by necessity and function. © 2007 Springer.

Process integration and improvement using systemic diagrams and a human-centred approach

If product cycle time reduction is the mission, and the multifunctional team is the means of achieving the mission, what then is the modus operandi by which this means is to accomplish its mission? This paper asserts that a preferred modus operandi for the multifunctional team is to adopt a process-oriented view of the manufacturing enterprise, and for this it needs the medium of a process map [16] The substance of this paper is a methodology which enables the creation of such maps Specific examples of process models drawn from the product develop ment life cycle are presented and described in order to support the methodology's integrity and value The specific deliverables we have so far obtained are a methodology for process capture and analysis, a collection of process models spanning the product development cycle, and, an engineering handbook which hosts these models and presents a computer-based means of navigating through these processes in order to allow users a better understanding of the nature of the business, their role in it, and why the job that they do benefits the work of the company We assert that this kind of thinking is the essence of concurrent engineering implementation, and further that the systemigram process models uniquely stim ulate and organise such thinking.

Discriminating antigen and non-antigen using proteome dissimilarity III:tumour and parasite antigens

Computational genome analysis enables systematic identification of potential immunogenic proteins within a pathogen. Immunogenicity is a system property that arises through the interaction of host and pathogen as mediated through the medium of a immunogenic protein. The overt dissimilarity of pathogenic proteins when compared to the host proteome is conjectured by some to be the determining principal of immunogenicity. Previously, we explored this idea in the context of Bacterial, Viral, and Fungal antigen. In this paper, we broaden and extend our analysis to include complex antigens of eukaryotic origin, arising from tumours and from parasite pathogens. For both types of antigen, known antigenic and non-antigenic protein sequences were compared to human and mouse proteomes. In contrast to our previous results, both visual inspection and statistical evaluation indicate a much wider range of homologues and a significant level of discrimination; but, as before, we could not determine a viable threshold capable of properly separating non-antigen from antigen. In concert with our previous work, we conclude that global proteome dissimilarity is not a useful metric for immunogenicity for presently available antigens arising from Bacteria, viruses, fungi, parasites, and tumours. While we see some signal for certain antigen types, using dissimilarity is not a useful approach to identifying antigenic molecules within pathogen genomes.

Multiwavelength fiber laser based on nonlinear polarization rotation

Multiwavelength fiber laser is a perfect light source for future wavelength-division-multiplexing optical communication systems. A multiwavelength fiber laser based on nonlinear polarization rotation with up to 18 wavelengths has been proposed and demonstrated. The intensity- and wavelength-dependent loss induced by nonlinear polarization rotation effect is used to alleviate the mode competition in the homogeneous broadening gain medium of erbium-doped fiber. Instead of traditional filters, a polarization-maintaining fiber is inserted into the laser cavity, with which the polarization-dependent isolator composes an equivalent Lyot birefringent fiber filter. The in-line birefringence fiber filter is used to simplify the laser configuration, which benefits systematic integration. The effect of the 980 nm pump power on the multiwavelength generation is investigated. It is shown that the pump power contributes a lot to the evenness of the multiwavelength spectra due to the intensity dependence of nonlinear polarization rotation effect.

Corporate cyberstalking:an invitation to build theory

Cyberstalking describes a relatively new form of stalking behaviour where technology is used as the medium of harassment. The term corporate cyberstalking is often used to describe incidents that involve organisations, such as companies and government departments. This paper uses a number of case studies in order to propose a typology of corporate cyberstalking. It is suggested that incidents involving corporate cyberstalking can be divided into two broad groups, depending on whether or not the organisation acts as a stalker or as a victim. Examining the motivations behind corporate cyberstalking allows these groups to be subdidvided further. The motives behind corporate cyberstalking can range from a desire for revenge against an employer to cyberterrorism. The paper also briefly discusses definitions of stalking and cyberstalking, concluding with a revised definition of cyberstalking that is more in keeping with the material discussed.

Multiwavelength fiber laser based on nonlinear polarization rotation

Multiwavelength fiber laser is a perfect light source for future wavelength-division-multiplexing optical communication systems. A multiwavelength fiber laser based on nonlinear polarization rotation with up to 18 wavelengths has been proposed and demonstrated. The intensity- and wavelength-dependent loss induced by nonlinear polarization rotation effect is used to alleviate the mode competition in the homogeneous broadening gain medium of erbium-doped fiber. Instead of traditional filters, a polarization-maintaining fiber is inserted into the laser cavity, with which the polarization-dependent isolator composes an equivalent Lyot birefringent fiber filter. The in-line birefringence fiber filter is used to simplify the laser configuration, which benefits systematic integration. The effect of the 980 nm pump power on the multiwavelength generation is investigated. It is shown that the pump power contributes a lot to the evenness of the multiwavelength spectra due to the intensity dependence of nonlinear polarization rotation effect.

McKinney, Mark. Redrawing French Empire Comics. Columbus, The Ohio State University Press, 2013. 304 pages. 9780814212202

The history of France and its empires is one that has been well trodden, particularly the French occupation, and subsequent war, in Algeria. In this companion to his earlier work, 2011’s The Colonial Heritage of French Comics,McKinney attempts to examine the reconstruction of French national identity in the wake of decolonisation through the medium of Francophonecomics. He endeavours to study the colonial affrontier (3), the space in which France and its colonies are connected and divided, where they seek to confront each other, or to seek peace and the removal of the division. McKinney argues this affrontier can be found most strongly in the Francophone comics produced dealing with the French colonial experience in Algeria, as well as that of Indochina,and does so from both sides of each conflict. McKinney examines in detail the French colonisation of Algeria (1830sonwards), the French war in Indochina (1946–54) and the Algerian war (1954–62), and his work is the first to approach these well-covered areas of research through the medium of comics. The resulting work takes the form of an investigation into the five forms of genealogical inquiry utilised in comics regarding these conflicts. His approach investigates the familial, ethnic, national, artistic and critical forms of genealogy relating to colonialism and imperialism from a variety of viewpoints, including the previously overlooked perspective of the pieds noirs. He aims to highlight both those cartoonists that critique the colonial ideology, as well as those cartoonists who to some extent attempt to gloss over or even romanticise the French empire, strengthening the affrontier. He positions himself alongside Foucault in seeing genealogy as a useful means of establishing ‘historical knowledge of struggles’ (Foucault1980, 85), but McKinney looks at the colonial representation in a popular medium,including the recent increase in comics produced which consider the French colonial experience. He argues that this consideration of the present, as well as European imperialism, is absent in the work of Foucault. The text is accompanied by a number of black and white facsimiles of pages from the comics he analyses to illustrate the different and often conflicting positions of cartoonists on these issues. Overall, McKinney’s work is a welcome addition to the study of the French colonial experience, which separates its elf from the rest by using Francophonecomics as lenses through which to look at these already well-trodden areas of study. He succeeds in determining if and how cartoonists critique colonial ideology and representations on both sides of the conflicts, a task in which he is unarguably successful. McKinney’s work, however, is unfortunately let down by typo graphicerrors, which occur throughout the text.Nevertheless, McKinney’s work is another important work in the field of Bande Dessine ́e scholarship, and useful for anyone interested in the representations of colonialism and imperialism in French comics, accompanied by anencyclopaedic bibliography of comics produced on this topic.