943 resultados para loop closure
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"May 1979."
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"B-277460"--P. 1.
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Statistics for 1865-74 are issued as section 1, v. 1-10, of Statistische bescheiden voor het Koningrijk der Nederlanden, published by the Departement van Binnenlandsche Zaken ("Loop der bevolking in 1860-1869" is section 3 of v. 5) Statistics for 1876-77 are issued as pt. 3, 1876-77, of Bijdragen tot de algemeene statistiek van Nederland, published by the Departement van Binnenlandsche Zaken. Statistics for 1900-22 are issued as no. 9, 21, 34, 44, 62, 77, 92, 109, 126, 142, 161, 178, 195, 207, 221, 236, 248, 263, 281, 307, 328, 355, 372 of Bijdragen tot de statistiek van Nederland, nieuwe volgreeks, published by the Centraal Bureau voor de Statistiek.
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Mode of access: Internet.
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"This fact sheet has been prepared pursuant to the requirements of Title 35 Illinois Administrative Code (35 IAC) Section 705.143. The fact sheet is intended to be a brief summary of the principal facts and significant factual, legal, methodological, and policy questions considered in preparing a draft Class 3 RCRA permit modification. This permit modification will allow the current permittee, Chevron Environmental Services Company (CESC), to establish an onsite Corrective Action Management Unit (CAMU) to manage remediation wastes generated during site remediation activities performed under the RCRA Corrective Action program and to establish a facility-wide Groundwater Management Zone (GMZ) for the duration of the corrective action work at the closed refinery. Pursuant to 35 IAC 705.143(a), this fact sheet is sent to the applicant, to the information repository and to any other person who requests it."
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Shipping list no.: 90-775-P.
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Includes index.
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"February 1967."
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"September 29, 2005."
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"July 1999."
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Thesis (doctoral)--Rijks-Universiteit te Leiden.
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Thesis (Ph.D.)--University of Washington, 2016-06
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The PotE protein is a putrescine-ornithine antiporter found in many gram-negative bacteria. It is a member of the APA family of transporters and has 12 predicted alpha-helical transmembrane spanning segments (TMS). While the substrate binding site has previously been mapped to a region near the surface of the cytoplasmic lipid layer, no structural feature within the periplasmic domains of PotE have been shown to be important for function. We examined the role of the only large outer loop, situated between transmembrane spanning segment 7 and 8, in putrescine uptake. Deletion of the highly conserved amino acids in the region closest to transmembrane spanning segment 7 produced a protein with little activity. Glycine-scanning mutagenesis of this region showed that Val(249) and Leu(254) were required for optimal transporter function. The V249G mutant transported putrescine at a lower maximal rate compared to wild-type (WT) but with the same substrate binding affinity. In contrast, the L254G mutant had a higher substrate affinity. A series of Val(249) mutants indicated that the hydrophobicity of this residue, which is located at or near the membrane surface, is important for PotE function. Secondary structure predictions of the large outer loop indicated the presence of a hydrophobic alpha-helix in the centre with a hydrophobic region at each end suggesting that the loop was not entirely exposed to the aqueous periplasmic space. The study shows that loop 7-8 is important for PotE function, possibly by forming a re-entrant loop in the channel of the transporter. (C) 2003 Elsevier Ltd. All rights reserved.
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The aim was to investigate the roles of transmembrane domain 2 and the adjacent region of the first intracellular loop in determining human noradrenaline transporter (hNET) function by pharmacological and substituted-cysteine accessibility method (SCAM) analyses. It was first necessary to establish a suitable background NET for SCAM. Alanine mutants of endogenous hNET cysteines, hC86A, hC131A and hC339A, were examined and showed no marked effects on expression or function. hNET and the mutants were also resistant to methanethiosulfonate (MTS), ethylammonium (MTSEA) and MTStrimethylammonium (MTSET). Hence, wild-type hNET is an appropriate background for production of cysteine mutants for SCAM. Pharmacological investigation showed that all mutants except hT99C and hL109C showed reduced cell-surface expression, while all except hM107C showed a reduction in functional activity. The mutations did not markedly affect the apparent affinities of substrates, but apparent affinities of cocaine were decreased 7-fold for hP97C and 10-fold for hF101C and increased 12-fold for hY98C. [H-3]Nisoxetine binding affinities were decreased 13-fold for hP97C and 5-fold for hF101C. SCAM analysis revealed that only hL102C was sensitive to 1.25 mM MTSEA, and this sensitivity was protected by noradrenaline, nisoxetine and cocaine. The results suggest that this region of hNET is important for interactions with antidepressants and cocaine, but it is probably not involved in substrate translocation mechanisms.