A subset of metzincins and related genes constitutes a marker of human solid organ fibrosis

Metzincins and functionally related genes play important roles in extracellular matrix remodeling both in healthy and fibrotic conditions. We recently presented a transcriptomic classifier consisting of 19 metzincins and related genes (MARGS) discriminating biopsies from renal transplant patients with or without interstitial fibrosis/tubular atrophy (IF/TA) by virtue of gene expression measurement (Roedder et al., Am J Transplant 9:517-526, 2009). Here we demonstrate that the same algorithm has diagnostic value in non-transplant solid organ fibrosis. We used publically available microarray datasets of 325 human heart, liver, lung, kidney cortex, and pancreas microarray samples (265 with fibrosis, 60 healthy controls). Expression of nine commonly differentially expressed genes was confirmed by TaqMan low-density arrays (Applied Biosystems, USA) in 50 independent archival tissue specimens with matched histological diagnoses to microarray patients. In separate and in combined, integrated microarray data analyses of five datasets with 325 samples, the previously published MARGS classifier for renal post-transplant IF/TA had a mean AUC of 87% and 82%, respectively. These data demonstrate that the MARGS gene panel classifier not only discriminates IF/TA from normal renal transplant tissue, but also classifies solid organ fibrotic conditions of human pancreas, liver, heart, kidney, and lung tissue samples with high specificity and accuracy, suggesting that the MARGS classifier is a cross-platform, cross-organ classifier of fibrotic conditions of different etiologies when compared to normal tissue.

Special Collections in the Classroom

Bucknell University's Special Collections/University Archives recently acquired various rare books that have been integrated into curricular initiatives.

Unpopular Archives

This article explores the “unpopular” archived life of Charles P. Daly, thirty-five-year president (1864–1899) of the New York–based American Geographical Society. This one-time highly prominent judge and civic leader popularized geography among professionals and the public alike. Daly’s popular geography, along with his subsequent containment within the archives, suggests explanations for his dismissal among geographical audiences of today. It is a useful and necessary exercise to trace the neglect of Daly within histories of geography and recapture him for today’s audiences, not only because of his influence on post–Civil War American geography but also because his story can shed light on how “disciplinary remembering” functions in geography.

Organizational Frames for Professional Claims: Private Military Corporations and the Rise of the Military Paraprofessional

Corporations, nongovernmental organizations, and other organizational forms are major players in the sodal world. Recently, sociological scholarship on organizations has converged with research on the professions to discuss the ways in which professions are shaped or influenced by different organizational forms. In this article, I borrows from the notion of framing within social movement research to argue that organizational forms frame the bids of aspiring professionals. More specifically, I argue that certain organizational forms-such as that of the modern corporation-can aid would-be professionals in making their claims for professional recognition. Organizations do this, I argue, by providing aspiring professionals with a ready-made setting, rationale, and guarantees that make the newcomers more easily recognizable as professionals to outside audiences. I explore this argument by examining how the corporate form has facilitated private military contractors in their attempts to legitimate and develop this highly controversial new industry. The data are drawn from my interviews with private military contractors, state officials, and other interested parties surrounding private military corporations, as well as from archival data that detail the rise of the private military industry.

qPCR-based mitochondrial DNA quantification: Influence of template DNA fragmentation on accuracy

Real-time PCR (qPCR) is the method of choice for quantification of mitochondrial DNA (mtDNA) by relative comparison of a nuclear to a mitochondrial locus. Quantitative abnormal mtDNA content is indicative of mitochondrial disorders and mostly confines in a tissue-specific manner. Thus handling of degradation-prone bioptic material is inevitable. We established a serial qPCR assay based on increasing amplicon size to measure degradation status of any DNA sample. Using this approach we can exclude erroneous mtDNA quantification due to degraded samples (e.g. long post-exicision time, autolytic processus, freeze-thaw cycles) and ensure abnormal DNA content measurements (e.g. depletion) in non-degraded patient material. By preparation of degraded DNA under controlled conditions using sonification and DNaseI digestion we show that erroneous quantification is due to the different preservation qualities of the nuclear and the mitochondrial genome. This disparate degradation of the two genomes results in over- or underestimation of mtDNA copy number in degraded samples. Moreover, as analysis of defined archival tissue would allow to precise the molecular pathomechanism of mitochondrial disorders presenting with abnormal mtDNA content, we compared fresh frozen (FF) with formalin-fixed paraffin-embedded (FFPE) skeletal muscle tissue of the same sample. By extrapolation of measured decay constants for nuclear DNA (λnDNA) and mtDNA (λmtDNA) we present an approach to possibly correct measurements in degraded samples in the future. To our knowledge this is the first time different degradation impact of the two genomes is demonstrated and which evaluates systematically the impact of DNA degradation on quantification of mtDNA copy number.

The Work and Workshop of the Master of the Rajhrad Altarpiece

The main aim of this project was to verify the possibility raised in the publication of new archival data in 1980 that the group of panel paintings attributed to the Master of the Rajhrad Altarpiece and his workshop were not created before 1420, as earlier academic opinion held, but in fact only around 1450. It included a detailed analysis of the 21 panels forming the group, including infrared reflectography research. This helped identify the highly personal underdrawing style of the "Rajhrad Master" as common to all the panels in the group, proving that they really form a coherent whole. Questions of the painter's education and the nature of Bohemian society around 1450 were also considered, as. Bartlova felt these had been neglected in earlier studies. She concludes that the Master studied in Prague in the late 1430s and then continued studying in Vienna and Munich from 1440. The new dating of the group of panels connected with this anonymous master and his workshop throws new light on Bohemian artistic production during the late Hussite period (c.1430-c.1470). It was generally thought that artistic activity moved out of Prague after the outbreak of the Hussite wars in the 1420s, but this research revealed considerable activity there throughout the period. Numerous painters listed in the Book of the Prague Painters' Guild for this period can now be linked with extant panels, although most of these have survived outside Prague, principally in South Bohemia. These findings have broad implications for studies of Bohemian art in this period.

Siberian Wooden Churches: Reality and Symbolics

The preservation of Siberia's cultural heritage poses a serious problem. Many Siberian churches were destroyed or reconstructed, without their original form being carefully described. Mainicheva studied the question of Siberian wooden churches of the 17th-18th centuries, producing a complex analysis of their symbolism, phenomenology and morphology. Published and archival materials show that such unique churches as the Sophia Cathedral in Tobolesk (1621), the Trinity Church in Tomsk (1654) and the Church of Kazan Holy Mother in Ilimsk (1679) directly reflected the main features of medieval Russian culture combined with new perceptions of Man and the Universe. These Russian Orthodox churches have considerable significance for understanding the natural development of Russian architecture as a part of the Russian culture of permanently moving people. All these churches, which no longer exist in their original form, were built by unknown folk builders and represented a good example of non-professional architecture. Mainicheva developed full descriptions of the churches, including graphic reconstructions of their original plans and facades.

Panel Discussion - Local Finns in Illinois

Finnish North American labor contributions and involvement in strikes such as the 1913-14 Michigan Copper Strike are being restored to the historical record and even commemorated; yet some Finnish American communities’ labor history still goes untold. We contend that in the case of DeKalb, Illinois, the Finnish American labor and strike history has been, in part, overshadowed in contemporary remembrance by the city’s promotion of traditional history and commemoration focused on the barbed wire barons. Local Finnish American labor involvement and participation in strikes appears to have been marginalized in favor of a subsequent historical narrative surrounding the capitalist entrepreneurship of elites. However, counter memories of labor struggles may be lost for a variety of reasons. External and internal forces make it difficult for marginalized groups to offer alternatives to the construction of collective memories that exclude them. These forces include, but are not limited to gradual assimilation into dominant culture, internal conflict within social movements, and fear of, or experience with, governmental repression. In our archival research, surveys and interviews with 2nd and 3rd generation Finnish American residents reveal the many forces of “forgetting” that can influence the counter memory of Finnish American labor history in certain communities.

Walter Friedländer (1891-1984) – Between Social Democracy and Social Darwinism

If the profession of social work is to have a future we must know where it came from, and the series of portraits of our pioneers is one of the paths into the origins of that profession. I feel grateful to the publishers for this online-journal and also honoured to be asked to continue the series on pioneers in social work. I gladly comply because, in connection with my research on Alice Salomon and other social workers who were expelled from Germany and other Nazi-occupied territories (Wieler1989 and 1995) I had the pleasure and privilege of meeting and interviewing Walter Friedländer shortly before he passed away. It is years ago that I visited him in his home among stacks of books and piles of papers. My memories are vivid. I still see his sparkling eyes and hear his soft voice with a very heavy German accent. I was most impressed by his memory of historical events and people which, it seemed, only a large hard-drive could retain these days. Now, I wish I had asked more questions but instead, we will have to rely largely on primary and secondary literature and box upon box of archival materials. I draw heavily on the comprehensive German and Jewish Intellectual Emigré Collection (http://library.albany.edu/speccoll/findaids/ger003.htm) which consists of nearly 50 cubic feet and another collection of the German Central Institute („Deutsches Zentralinstitut für Soziale Fragen-DZI“) in Berlin (www.dzi.de). Some of the more current archival materials were lost in a flood, and much of Friedländer’s early memorabilia up to 1933 was lost in Germany. There are also internet resources with widely differing information. I hope that I will not have overlooked too much in order to do justice to this remarkable pioneer and colleague. In order to appreciate and pay tribute to Walter Friedländer and his contributions we will have to reconsider the historical and international context of more than the 93 years of his life span: the German Monarchy, the Weimar Republic, Nazi-Fascism, Swiss, French and American exile and numerous visits to other countries.

Target difficulty, intra-year target revisions and firm performance: evidence from business units' targets

Target difficulty is often argued to increase performance. While this association is well established in experimental research, empirical evidence in field research is rather mixed. We attempt to explain this inconsistency by analyzing the importance of intra-year target revisions, which are especially prevalent in real-world field settings. Using survey and archival data from 97 firms, we find that firms with more challenging business unit targets revise targets more often, in line with asymmetric, downward target revisions. Results further show that the degree to which targets are revised during a period results in negative effects on firm performance, as the anticipation of revision negatively affects the business unit management’s performance incentives. Additionally, we find that using targets predominantly for either decision-making or control influences the overall performance effects of target revisions. Our findings may partially explain the mixed field study evidence regarding the effects of target difficulty.

Cloth-covered Clamshell Music Box

Unbound sheet music and an audio CD of a live performance of the sheet music. GoalTo design an aesthetically cohesive binding and housing for the materials. TreatmentThe sheet music was bound as a double-fan adhesive with cloth-covered case binding. The bound volume and audio CD were housed together in a cloth-covered clamshell box with Volara foam insert.

Beanie Box

Iowa State University freshman beanie, c. 1918. GoalTo design a housing which would support and protect the beanie, and would also allow the beanie to be viewed without being handled. TreatmentA custom, cloth-covered box with Velcro-supported, drop-down sides was constructed to fit the dimensions of a museum-quality hat stand.

Sink mat for broken glass plate negative

Broken glass plate negative. Goal To design a simple protective enclosure for the two pieces of the glass plate negative, that allows the user to visualize the image as a whole. Treatment A sink mat was created by layering museum board and Volera foam, and "sinks" cut to fit the broken pieces along with thumb notches for ease of lifting. A portfolio of e-flute board, buckram, and cotton ties was built up around the sink mat to provide a protective enclosure that is easily stored on edge.

Clamshell box for miniature book

Miniature artist's book. Goal To create standard size housing for miniature books so that they can be shelved with the collection. Treatment Based on a standard size, a clamshell box with foam filler was created to house a miniature artist's book. Cotton tape secured beneath the foam was added to gently lift the book from the foam insert.