Assessment of the effects of ozone exposure and plant competition on the reproductive ability of three therophytic clover species from Iberian pastures

Ozone (O3) phytototoxicity has been reported on a wide range of crops and wild Central European plantspecies, however no information has been provided regarding the sensitivity of plantspecies from dehesa Mediterranean therophytic grasslands in spite of their great plantspecies richness and the high O3 levels that are recorded in this area. A study was carried out in open-top chambers (OTCs) to assess the effects of O3 and competition on the reproductiveability of threecloverspecies: Trifolium cherleri, Trifolium subterraneum and Trifolium striatum. A phytometer approach was followed, therefore plants of these species were grown in mesoscosms composed of monocultures of four plants of each species, of threeplants of each species competing against a Briza maxima individual or of a single plant of each cloverspecies competing with threeB. maximaplants. Three O3 treatments were adopted: charcoal filtered air (CFA), non-filtered air (NFA) and non-filtered air supplemented with 40 nl l−1 of O3 (NFA+). The different mesocosms were exposed to the different O3 treatments for 45 days and then they remained in the open. Ozoneexposure caused reductions in the flower biomass of the threecloverspecies assessed. In the case of T. cherleri and T. subterraneum this effect was found following their exposure to the different O3 treatments during their vegetative period. An attenuation of these effects was found when the plants remained in the open. Ozone-induced detrimental effects on the seed output of T. striatum were also observed. The flower biomass of the cloverplants grown in monocultures was greater than when competing with one or threeB. maxima individuals. An increased flower biomass was found in the CFA monoculture mesocosms of T. cherleri when compared with the remaining mesocosms, once the plants were exposed in the open for 60 days. The implications of these effects on the performance of dehesa acid grasslands and for the definition of O3 critical levels is discussed

The human side of social technology for climate change mitigation and human development: the case of "efficient stoves" in Brazil

Carbon management has gradually gained attention within the overall environmental management and corporate social responsibility agendas. The clean development mechanism, from Kyoto Protocol, was envisioned as connecting carbon market and sustainable development objectives in developing countries. Previous research has shown that this potential is rarely being achieved. The paper explores how the incorporation of the human side into carbon management reinforces its contribution to generate human development in local communities and to improve the company's image. A case study of a Brazilian company is presented, with the results of the application of an analytical model that incorporates the human side and human development. The selected project is an "efficient stoves" programme. "Efficient stoves" are recognised in Brazil as social technologies. Results suggest that the fact that social technologies value the human side of the technology plays a key role when it comes to analysing the co-benefits of the project implementation.

Differences between innovative and non-innovative microenterprises: internal factors

This exploratory study presents a comparison between two samples of microenterprises. One sample is formed by companies involved in product innovation during the current economic crisis and the other is formed by companies not involved in product innovation during the same period. The comparison analyzes which internal factors, supported by the literature as the influential factors of small business innovation, are significant when explaining the main differences between innovative microenterprise and non-innovative ones. The results suggest that the factors related to the organization and activity of the company are the factors which explain the differences between these two types of firms, rather than those factors related to micro-entrepreneurs own profile.

Tecnología y equipos para redes ultrarrápidas

Este proyecto de fin de carrera se realiza bajo la supervisión y aprobación de la empresa BT S.A.U. Este documento pretende ser un manual básico para dar a conocer al lector los elementos, herramientas y procedimientos para llevar a cabo un proyecto de tendido de fibra óptica en España. Se compone de 5 temas cuyos contenidos paso a resumir a continuación. Tema 1. En él, se muestra una explicación a las redes de acceso y las distintas topologías FTTx necesarias para llegar al cliente, además de las tecnologías y elementos utilizados para su conexión. Tema 2. Se explicará la regulación española para el tendido de redes de acceso necesarios como son la Regulación OBA, coubicación en las centrales de Telefónica, y la Regulación MARCO, compartición de la infraestructura de Telefónica con otros operadores. Tema 3. Aquí se explica la herramienta NEON, necesaria para la “comunicación” con Telefónica para las peticiones de la compartición de su infraestructura y un apartado para la legislación municipal para el despliegue de redes de fibra. Tema 4. Expondremos el procedimiento de trabajo en campo. Hablaremos de los procedimientos del tendido de cable, las obras civiles, y finalmente las medidas para comprobar el enlace. Tema 5. Explicaremos un ejemplo de despliegue real, desde la viabilidad hasta finalmente el tendido. Tema 6. Por último, expondré mis conclusiones y trabajos futuros. Este proyecto está orientado desde un punto de vista de la infraestructura (Capa Física, Modelo OSI). ABSTRACT. This thesis project is carried out under the supervision and approval of the company BT S.A.U. This document is intended as a basic manual to expose the reader items, tools, and procedures to carry out a deployment of optical fiber project in Spain. It is composed of 5 topics whose contents I summarize below. Topic 1. In it, shows an explanation to access networks and different topologies FTTx necessary to reach the customer, as well as technologies and elements used for connection. Topic 2. In this topic will be explained the Spanish regulation for the access networks needed such as the regulation of OBA, co-location in Telefonica’s central, and the framework regulation, sharing of Telefonica infrastructure with other operators. Topic 3. Here we explain the tool NEON, necessary for the "communication" with Telefónica for requests for infrastructure sharing, and a section for municipal legislation for the deployment of fiber networks. Topic 4. We will exhibit work in field procedure. We will discuss the procedures of laying cable, civil works, and finally measures to check the link. Topic 5. We will present an example of an actual deployment, from viability until finally laying. Topic 6. Finally, I shall explain my conclusions and future work. This project is from a point of view of the infrastructure (physical layer, OSI model).

Irrigation efficiency and water-policy implications for river-basin resilience

Rising demand for food, fiber, and biofuels drives expanding irrigation withdrawals from surface water and groundwater. Irrigation efficiency and water savings have become watchwords in response to climate-induced hydrological variability, increasing freshwater demand for other uses including ecosystem water needs, and low economic productivity of irrigation compared to most other uses. We identify three classes of unintended consequences, presented here as paradoxes. Ever-tighter cycling of water has been shown to increase resource use, an example of the efficiency paradox. In the absence of effective policy to constrain irrigated-area expansion using "saved water", efficiency can aggravate scarcity, deteriorate resource quality, and impair river basin resilience through loss of flexibility and redundancy. Water scarcity and salinity effects in the lower reaches of basins (symptomatic of the scale paradox) may partly be offset over the short-term through groundwater pumping or increasing surface water storage capacity. However, declining ecological flows and increasing salinity have important implications for riparian and estuarine ecosystems and for non-irrigation human uses of water including urban supply and energy generation, examples of the sectoral paradox. This paper briefly considers three regional contexts with broadly similar climatic and water-resource conditions – central Chile, southwestern US, and south-central Spain – where irrigation efficiency directly influences basin resilience. The comparison leads to more generic insights on water policy in relation to irrigation efficiency and emerging or overdue needs for environmental protection.

Afetividade e Fluência na Interação Empresarial: Um estudo sobre Mediação

A partir de conflito interpessoal entre gestores de empresa de médio porte, esta pesquisa realiza estudo de caso com objetivo de investigar os reflexos da mediação na fluência de interação e na afetividade dentro da empresa, enfocando 19 participantes, em três níveis: diretoria, equipe da diretoria e coordenadores. Tem como sustentação teórica as abordagens de mediação: Tradicional de Harvard; Transformativa; Narrativo-Circular e Facilitação, delineadas a partir do modelo dos sistemas dinâmicos da Teoria da Complexidade. Após caracterização inicial da empresa, utiliza-se de técnicas de pré-mediação, mediação e facilitação em grupo, analisando-as qualitativamente. Com preocupação sobre a racionalidade dos resultados sobre os reflexos do trabalho de mediação, compõe questionário sobre fluência de interação e afetividade na empresa, QFI. Os resultados do questionário comprovam os da análise da mediação, sendo que 51% dos funcionários assinalam alterações positivas na interação e na afetividade na empresa como um todo. Os pontos nevrálgicos, apontados pelos participantes como reformulados na mediação referem-se a: Autoritarismo; Muita Pressão; Falta Transparência; Co-Responsável; Cisão entre as áreas Administrativa e Técnica (Cisão AA-AT); Centralização e Escuta Insuficiente. Os dados indicam uma abertura sistêmica na tomada de consciência dos conflitos, associada a uma maior responsabilidade conjunta em tentar resolvê-los, através do gerenciamento integrado e dinâmico de competências individuais, intra e inter-grupais na empresa. O estudo considera, portanto, que a mediação pode ser vista como uma abordagem alternativa de resolução de conflitos, com resultados positivos ao meio organizacional. Devido ao fato de as técnicas de mediação não estarem ainda muito difundidas em nossa realidade, recomenda a necessidade de novas pesquisas , diversificando seu foco em empresas de vários tamanhos e segmentos.

Afetividade e Fluência na Interação Empresarial: Um estudo sobre Mediação

A partir de conflito interpessoal entre gestores de empresa de médio porte, esta pesquisa realiza estudo de caso com objetivo de investigar os reflexos da mediação na fluência de interação e na afetividade dentro da empresa, enfocando 19 participantes, em três níveis: diretoria, equipe da diretoria e coordenadores. Tem como sustentação teórica as abordagens de mediação: Tradicional de Harvard; Transformativa; Narrativo-Circular e Facilitação, delineadas a partir do modelo dos sistemas dinâmicos da Teoria da Complexidade. Após caracterização inicial da empresa, utiliza-se de técnicas de pré-mediação, mediação e facilitação em grupo, analisando-as qualitativamente. Com preocupação sobre a racionalidade dos resultados sobre os reflexos do trabalho de mediação, compõe questionário sobre fluência de interação e afetividade na empresa, QFI. Os resultados do questionário comprovam os da análise da mediação, sendo que 51% dos funcionários assinalam alterações positivas na interação e na afetividade na empresa como um todo. Os pontos nevrálgicos, apontados pelos participantes como reformulados na mediação referem-se a: Autoritarismo; Muita Pressão; Falta Transparência; Co-Responsável; Cisão entre as áreas Administrativa e Técnica (Cisão AA-AT); Centralização e Escuta Insuficiente. Os dados indicam uma abertura sistêmica na tomada de consciência dos conflitos, associada a uma maior responsabilidade conjunta em tentar resolvê-los, através do gerenciamento integrado e dinâmico de competências individuais, intra e inter-grupais na empresa. O estudo considera, portanto, que a mediação pode ser vista como uma abordagem alternativa de resolução de conflitos, com resultados positivos ao meio organizacional. Devido ao fato de as técnicas de mediação não estarem ainda muito difundidas em nossa realidade, recomenda a necessidade de novas pesquisas , diversificando seu foco em empresas de vários tamanhos e segmentos.

Ethnohistory, genetics, and cancer mortality in Europeans

Geographic variation in cancer rates is thought to be the result of two major factors: environmental agents varying spatially and the attributes, genetic or cultural, of the populations inhabiting the areas studied. These attributes in turn result from the history of the populations in question. We had previously constructed an ethnohistorical database for Europe since 2200 B.C., permitting estimates of the ethnic composition of modern European populations. We were able to show that these estimates correlate with genetic distances. In this study, we wanted to see whether they also correlate with cancer rates. We employed two data sets of cancer mortalities from 42 types of cancer for the European Economic Community and for Central Europe. We subjected spatial differences in cancer mortalities, genetic, ethnohistorical, and geographic distances to matrix permutation tests to determine the magnitude and significance of their association. Our findings are that distances in cancer mortalities are correlated more with ethnohistorical distances than with genetic distances. Possibly the cancer rates may be affected by loci other than the genetic systems available to us, and/or by cultural factors mediated by the ethnohistorical differences. We find it remarkable that patterns of frequently ancient ethnic admixture are still reflected in modern cancer mortalities. Partial correlations with geography suggest that local environmental factors affect the mortalities as well.

Delayed symptom onset and increased life expectancy in Sandhoff disease mice treated with N-butyldeoxynojirimycin

Sandhoff disease is a neurodegenerative disorder resulting from the autosomal recessive inheritance of mutations in the HEXB gene, which encodes the β-subunit of β-hexosaminidase. GM2 ganglioside fails to be degraded and accumulates within lysosomes in cells of the periphery and the central nervous system (CNS). There are currently no therapies for the glycosphingolipid lysosomal storage diseases that involve CNS pathology, including the GM2 gangliosidoses. One strategy for treating this and related diseases is substrate deprivation. This would utilize an inhibitor of glycosphingolipid biosynthesis to balance synthesis with the impaired rate of catabolism, thus preventing storage. One such inhibitor is N-butyldeoxynojirimycin, which currently is in clinical trials for the potential treatment of type 1 Gaucher disease, a related disease that involves glycosphingolipid storage in peripheral tissues, but not in the CNS. In this study, we have evaluated whether this drug also could be applied to the treatment of diseases with CNS storage and pathology. We therefore have treated a mouse model of Sandhoff disease with the inhibitor N-butyldeoxynojirimycin. The treated mice have delayed symptom onset, reduced storage in the brain and peripheral tissues, and increased life expectancy. Substrate deprivation therefore offers a potentially general therapy for this family of lysosomal storage diseases, including those with CNS disease.

Agrin in Alzheimer’s disease: Altered solubility and abnormal distribution within microvasculature and brain parenchyma

Agrin is a heparan sulfate proteoglycan that is widely expressed in neurons and microvascular basal lamina in the rodent and avian central nervous system. Agrin induces the differentiation of nerve-muscle synapses, but its function in either normal or diseased brains is not known. Alzheimer’s disease (AD) is characterized by loss of synapses, changes in microvascular architecture, and formation of neurofibrillary tangles and senile plaques. Here we have asked whether AD causes changes in the distribution and biochemical properties of agrin. Immunostaining of normal, aged human central nervous system revealed that agrin is expressed in neurons in multiple brain areas. Robust agrin immunoreactivity was observed uniformly in the microvascular basal lamina. In AD brains, agrin is highly concentrated in both diffuse and neuritic plaques as well as neurofibrillary tangles; neuronal expression of agrin also was observed. Furthermore, patients with AD had microvascular alterations characterized by thinning and fragmentation of the basal lamina. Detergent extraction and Western blotting showed that virtually all the agrin in normal brain is soluble in 1% SDS. In contrast, a large fraction of the agrin in AD brains is insoluble under these conditions, suggesting that it is tightly associated with β-amyloid. Together, these data indicate that the agrin abnormalities observed in AD are closely linked to β-amyloid deposition. These observations suggest that altered agrin expression in the microvasculature and the brain parenchyma contribute to the pathogenesis of AD.

Heterogeneity of subcellular localization and electrophoretic mobility of survival motor neuron (SMN) protein in mammalian neural cells and tissues

Spinal muscular atrophy is caused by defects in the survival motor neuron (SMN) gene. To better understand the patterns of expression of SMN in neuronal cells and tissues, we raised a polyclonal antibody (abSMN) against a synthetic oligopeptide from SMN exon 2. AbSMN immunostaining in neuroblastoma cells and mouse and human central nervous system (CNS) showed intense labeling of nuclear “gems,” along with prominent nucleolar immunoreactivity in mouse and human CNS tissues. Strong cytoplasmic labeling was observed in the perikarya and proximal dendrites of human spinal motor neurons but not in their axons. Immunoblot analysis revealed a 34-kDa species in the insoluble protein fractions from human SY5Y neuroblastoma cells, embryonic mouse spinal cord cultures, and human CNS tissue. By contrast, a 38-kDa species was detected in the cytosolic fraction of SY5Y cells. We conclude that SMN protein is expressed prominently in both the cytoplasm and nucleus in multiple types of neurons in brain and spinal cord, a finding consistent with a role for SMN as a determinant of neuronal viability.

Roles for genomic imprinting and the zygotic genome in placental development

The placenta contains several types of feto-maternal interfaces where zygote-derived cells interact with maternal cells or maternal blood for the promotion of fetal growth and viability. The genetic factors regulating the interactions between different cell types within feto-maternal interfaces and the relative contributions of the maternal and zygotic genomes are poorly understood. Genomic imprinting, the epigenetic process responsible for parental origin-dependent functional differences between homologous chromosomes, has been proposed to contribute to these events. Previous studies showed that mouse conceptuses with an absence of imprinted differences between the two copies of chromosome 12 (upon paternal inheritance of both copies) die late in gestation and have a variety of defects, including placentomegaly. Here we examined the role of chromosome 12 imprinting in these placentae in more detail. We show that the spatial interactions between different cell types within feto-maternal interfaces are defective and identify abnormal behaviors in both zygote-derived and maternal cells that are attributed to the genome of the zygote but not the mother. These include compromised invasion of the maternal decidualized endometrium and the central maternal artery situated within it by zygote-derived trophoblast, abnormalities in the wall of the central maternal artery, and defects within the zygote-derived cellular layer of the labyrinth, which is in direct contact with maternal blood. These findings demonstrate multiple roles for chromosome 12 imprinting in the placenta that have not previously been associated with imprinting effects. They provide insights into the function of imprinting in placental development and have evolutionary and clinical implications.

The spinal biology in humans and animals of pain states generated by persistent small afferent input

Behavioral models indicate that persistent small afferent input, as generated by tissue injury, results in a hyperalgesia at the site of injury and a tactile allodynia in areas adjacent to the injury site. Hyperalgesia reflects a sensitization of the peripheral terminal and a central facilitation evoked by the persistent small afferent input. The allodynia reflects a central sensitization. The spinal pharmacology of these pain states has been defined in the unanesthetized rat prepared with spinal catheters for injection and dialysis. After tissue injury, excitatory transmitters (e.g., glutamate and substance P) acting though N-methyl-d-aspartate (NMDA) and neurokinin 1 receptors initiate a cascade that evokes release of (i) NO, (ii) cyclooxygenase products, and (iii) activation of several kinases. Spinal dialysis show amino acid and prostanoid release after cutaneous injury. Spinal neurokinin 1, NMDA, and non-NMDA receptors enhance spinal prostaglandin E2 release. Spinal prostaglandins facilitate release of spinal amino acids and peptides. Activation by intrathecal injection of receptors on spinal C fiber terminals (μ,/∂ opiate, α2 adrenergic, neuropeptide Y) prevents release of primary afferent peptides and spinal amino acids and blocks acute and facilitated pain states. Conversely, consistent with their role in facilitated processing, NMDA, cyclooxygenase 2, and NO synthase inhibitors act to diminish only hyperalgesia. Importantly, spinal delivery of several of these agents diminishes human injury pain states. This efficacy emphasizes (i) the role of facilitated states in humans, (ii) shows the importance of spinal systems in human pain processing, and (iii) indicates that these preclinical mechanisms reflect processes that regulate the human pain experience.

BDNF but not NT-4 is required for normal flexion reflex plasticity and function

Neurotrophins can directly modulate the function of diverse types of central nervous system synapses. Brain-derived neurotrophic factor (BDNF) might be released by nociceptors onto spinal neurons and mediate central sensitization associated with chronic pain. We have studied the role of BDNF and neurotrophin-4 (NT-4), both ligands of the trkB tyrosine kinase receptor, in synaptic transmission and reflex plasticity in the mouse spinal cord. We used an in vitro spinal cord preparation to measure monosynaptic and polysynaptic reflexes evoked by primary afferents in BDNF- and NT-4-deficient mice. In situ hybridization studies show that both these neurotrophins are synthesized by sensory neurons, and NT-4, but not BDNF, also is expressed by spinal neurons. BDNF null mutants display selective deficits in the ventral root potential (VRP) evoked by stimulating nociceptive primary afferents whereas the non-nociceptive portion of the VRP remained unaltered. In addition, activity-dependent plasticity of the VRP evoked by repetitive (1 Hz) stimulation of nociceptive primary afferents (termed wind-up) was substantially reduced in BDNF-deficient mice. This plasticity also was reduced in a reversible manner by the protein kinase inhibitor K252a. Although the trkB ligand NT-4 is normally present, reflex properties in NT-4 null mutant mice were normal. Pharmacological studies also indicated that spinal N-methyl-d-aspartate receptor function was unaltered in BDNF-deficient mice. Using immunocytochemistry for markers of nociceptive neurons we found no evidence that their number or connectivity was substantially altered in BDNF-deficient mice. Our data therefore are consistent with a direct role for presynaptic BDNF release from sensory neurons in the modulation of pain-related neurotransmission.