Temporal and Spatial Scaling of the Genetic Structure of a Vector-Borne Plant Pathogen

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Population Structure and Pathotype Diversity of the Wheat Blast Pathogen Magnaporthe oryzae 25 Years After Its Emergence in Brazil

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Pathogen levels and clinical response to periodontal treatment in patients with Interleukin 8 haplotypes

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

High genetic diversity among Pseudomonas aeruginosa and Acinetobacter spp. isolated in a public hospital in Brazil

In Brazil and other regions of the world, Pseudomonas aeruginosa and Acinetobacter spp. have emerged as important agents of nosocomial infection and are commonly involved in outbreaks. The main objective of the present study was to evaluate the genetic relationship among P. aeruginosa and Acinetobacter spp. isolated from patients in a public university hospital in northwestern Parana, Brazil, and report their antimicrobial resistance profile. A total of 75 P. aeruginosa and 94 Acinetobacter spp. isolates were phenotypically identified and tested for antibiotic susceptibility using automated methodology. Polymyxin B was tested by disk diffusion for P. aeruginosa. Metallo-beta-lactamase (MBL) was detected using a disk approximation test. Genotyping was performed using enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR). Approximately 55% of the P. aeruginosa isolates and 92% of the Acinetobacter spp. isolates were multiresistant, but none were MBL-producers. ERIC-PCR revealed the presence of small clusters of carbapenem-resistant Acinetobacter spp., most likely OXA-type carbapenemase producers. Furthermore, high genetic diversity in P. aeruginosa and Acinetobacter spp. clinical isolates was observed, suggesting that cross-transmission is not very frequent in the studied hospital.

Comparação de suínos convencionais e SPF (specific pathogen free)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Effects of UV-B radiation on Lecanicillium spp., biological control agents of the coffee leaf rust pathogen

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Hiperacumulação de cádmio em plântulas de Cajanus cajan inoculadas com Pseudomonas

Pós-graduação em Agronomia (Genética e Melhoramento de Plantas) - FCAV

Formulation of Pseudomonas chlororaphis strains for improved shelf life

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Partitioning the net effect of host diversity on an emerging amphibian pathogen

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Simulating Population Genetics of Pathogen Vectors in Changing Landscapes: Guidelines and Application with Triatoma brasiliensis

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Rhamnolipids Production by a Pseudomonas eruginosa LBI Mutant: Solutions and Homologs Characterization

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Produção e aplicação de ramnolipídios produzidos por Pseudomonas aeruginosa LBI 2A1

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Avaliação do efeito do tratamento com surfactante químico e Pseudomonas aeruginosa LBI na toxicidade de solo contaminado com óleo lubrificante usado

Petroleum and its subproducts are considered a treat for the environmental quality because of the many environmental accidents that may occur during exploitation, transport and storage. A common remediation technique used in the contaminated areas is based on the use of surfactants, mainly the chemical ones, because they have low production costs. In the other hand, some microorganisms have indicate capacities of producing surfactants that emulsify substances and as result, offer a bigger contact surface for the microbiota degradation. This biossurfactants stand out in comparison with the chemical surfactants because they present lower micelar concentration values, are more tolerant for temperature and pH variation, because they are biodegradable, have low toxicity, higher emulsification and hydrocarbon solubilization index. In this way, after the surfactant application, a toxicity evaluation have to be made to identify the treatment effects. In soil, the activity of some microbial enzymes can show the environmental behavior of the contaminant under different treatment conditions. Dehydrogenase is one example of those enzymes that can demonstrate indirectly the effect of the pollutant on the soil microorganisms. The aim of this paper was to evaluate the toxicity after the addition of a surfactant and/or Pseudomonas aeruginosa LBI in soil contaminated by a mineral automotive lubricant. The previous mentioned bacteria are a potential biossurfactant (rhamnolipid) producer. In order to evaluate the toxicity, the dehydrogenase test was run. In this test, trifeniltetrazolium compound (TTC) after utilized as an electron acceptor, turns into trifenil formazan (TPF), that can be indirectly quantified using the absorbance measured by the spectrophotometer UV-visible. In this way, it was possible to quantify the dehydrogenase activity from the contaminated soil samples... (Complete abstract click electronic access below)

Caracterização de Bacteriófagos Infectando Isolados Clínicos de Pseudomonas aeruginosa Armazenados em uma Coleção de Cultura

To isolate, to concentrate and to purify bacteriophages from isolates of P. aeruginosa; To observe the capacity of bacteriophages to infect isolates of P. aeruginosa susceptible and multiresitant to antimicrobial; To caractherize bacteriphages by electronic microscopy techniques. 10 isolates of Pseudomonas aeruginosa from LEMC culture collection were submitted to the experiments of ideal temperature for the lyse region appearance in the MaConkey culture plate and 2 extraction methods for the concentration of the phages, clorophorm (Silankorva) and filtration plus centrifugation (Bergan). Three infected clinical isolates of multiresistant P. aeruginosa an one susceptible isolate ( PA01) were evaluated by 3 transmission electron microscopy techniques to caractherize phages morphologically (“on grid”, “on drop” and direct extraction from the lyse region of the culture plate). The ideal temperature to obtain lyses region was 37°C. The stock solutions, obtained through the methodologies of Sillankorva and Bergan, had satisfactory results in infecting the multiresistant isolate and the negative control. Among the 3 techniques of electronic microscopy tested the direct from the lyse plate was the best to obtain the micrography of the phages

Estudo dos micronutrientes na produção de ramnolipídios por mutante de Pseudomonas aeruginosa LBI

Os biossurfactantes apresentam inúmeras vantagens, como baixa toxicidade, biodegradabilidade e alta estabilidade, mas não são amplamente utilizados devido ao custo de produção. A utilização de substratos baratos, linhagens mutantes que associados à otimização das condições de cultivo pode levar a uma redução nos custos, possibilitando assim a substituição dos surfactantes sintéticos pelos biológicos. Uma maneira empregada para maximizar a produção dos biossurfactantes é a limitação de nutrientes. Os esforços empregados nesse sentido são direcionado para as proporções carbono: nitrogênio, entretanto os efeitos dos elementos traços são pouco conhecidos. Devido a esses fatores, o presente trabalho avaliou a importância dos seguintes elementos traços: ferro, zinco, cobalto, cobre, manganês e do sal citrato de sódio dihidratado, nas fermentações realizadas utilizando o mutante de Pseudomonas aeruginosa LBI 2A1. Para tanto foram realizadas fermentações em frascos Erlenmeyer, onde se utilizou diferentes concentrações desses elementos. A influência dos mesmos na produção de ramnolipídios foi constatada, uma vez que a produção desse biotensoativo foi aumentada em mais de três vezes alterando apenas a concentração de um único elemento traço (Fe). Os experimentos realizados permitem, também, inferir a respeito das melhores concentrações desses micronutrientes para a produção de ramnolipídios