Hormonal regulation of (Na+,K+)-ATPase biosynthesis in the toad bladder. Effect of aldosterone and 3,5,3'-triiodo-L-thyronine.

Aldosterone stimulates transepithelial Na+ transport in the toad bladder, and thyroid hormone antagonizes this mineralocorticoid action. In the present study, we assessed the influence of these two hormones on the biosynthesis of (Na+,K+)ATPase, the major driving force of Na+ transport. Rates of enzyme synthesis were estimated by immunoprecipitation with monospecific alpha (96,000 daltons) and beta (60,000 daltons) subunit antibodies. After a 30-min pulse of intact tissue with [35S]methionine, the anti-alpha-serum recognized the 96,000-dalton alpha subunit and the anti-beta-serum, a 42,000-dalton protein, in total cell extracts. The biosynthesis rates of both these proteins were increased 2.8- and 2.4-fold respectively, over controls by 80 nM aldosterone after 18 h of hormone treatment. The hormonal effect was not apparent up to 3 h of incubation and was dose dependent between 0.2 and 20 nM aldosterone. The hormonal induction was antagonized by spironolactone (500-fold excess) but not by amiloride. The action of aldosterone thus seems to be a receptor-mediated process and a primary event independent of the Na+ permeability of the apical membrane. Thyroid hormone, on the other hand, had no effect on either basal or aldosterone-stimulated synthesis rates of both enzyme proteins. The results demonstrate a direct effect of aldosterone on gene expression of the (Na+,K+)-ATPase. Ultimately, this phenomenon could be linked to the late mineralocorticoid action of this hormone. On the other hand, thyroid hormone, in contrast to the situation in mammals, does not stimulate de novo enzyme synthesis in amphibia. Neither can the antimineralocorticoid action of thyroid hormone in the toad bladder be explained by an inhibition of the (Na+,K+)-ATPase synthesis.

Copy number variation in the porcine genome inferred from a 60 k SNP BeadChip

Background: Recent studies in pigs have detected copy number variants (CNVs) using the Comparative Genomic Hybridization technique in arrays designed to cover specific porcine chromosomes. The goal of this study was to identify CNV regions (CNVRs) in swine species based on whole genome SNP genotyping chips. Results: We used predictions from three different programs (cnvPartition, PennCNV and GADA) to analyze data from the Porcine SNP60 BeadChip. A total of 49 CNVRs were identified in 55 animals from an Iberian x Landrace cross (IBMAP) according to three criteria: detected in at least two animals, contained three or more consecutive SNPs and recalled by at least two programs. Mendelian inheritance of CNVRs was confirmed in animals belonging to several generations of the IBMAP cross. Subsequently, a segregation analysis of these CNVRs was performed in 372 additional animals from the IBMAP cross and its distribution was studied in 133 unrelated pig samples from different geographical origins. Five out of seven analyzed CNVRs were validated by real time quantitative PCR, some of which coincide with well known examples of CNVs conserved across mammalian species. Conclusions: Our results illustrate the usefulness of Porcine SNP60 BeadChip to detect CNVRs and show that structural variants can not be neglected when studying the genetic variability in this species.

Metabolic responses of guava trees irrigated withdifferent N and K levels in São Francisco Valley

The guava (Psidium guajava L.) cv. Paluma has been cultivated in São Francisco Valley, Northeastern of Brazil, for in natura consumption and processing purposes. In spite of its importance, there are few scientific knowledge regarding guava physiology, nutrition, irrigation and fertigation. The objective of this work was to evaluate the effect of weather conditions and different concentrations of N and K applied by fertigation in foliar contents of reducing sugars, total soluble sugars, starch, sucrose, amino acids, and proteins. The field experiment was carried out at Bebedouro Experimental Field and the biochemical evaluations at the Laboratory of Seed and Plant Physiology, both located at Embrapa Semi-Árido, Petrolina-PE. The doses of 200 g N and 100 g K2O; 400 g N and 200 g K2O; 600 g N and 300 g K2O; and 800 g N and 400 g K2O per plant were applied in an experiment field. The experimental design was totally randomized blocks, with four treatments and five blocks. The weather conditions influenced the plant photosynthesis, which affects the plants metabolism. Guava presented specific responses to N and K fertigation for each parameter evaluated. The weather conditions during the evaluation period influenced guava responses to N and K fertigation.

Fertirrigação da bananeira cv. Prata-Anã com N e K em um argissolo vermelho-amarelo

O Nordeste brasileiro é a principal região produtora de banana, responsável por cerca de 35,80% da produção nacional. Apesar das condições favoráveis ao seu cultivo, a produtividade tem sido muito abaixo do seu potencial. Geralmente, isso se deve a problemas de suprimento hídrico e nutricional. Dentre os nutrientes, o nitrogênio (N) e o potássio (K) são requeridos em maiores quantidades pela bananeira. Com o objetivo de estudar o efeito de quatro doses de N (0; 117; 235 e 352 kg ha-1 de N) e de K (0; 157; 313 e 470 kg ha-1 de K2O), aplicados via fertirrigação, avaliaram-se características produtivas de um 2º ciclo de cultivo da banana cv. Prata-Anã, em um Argissolo Vermelho-Amarelo dos tabuleiros costeiros do Nordeste brasileiro. O N não influenciou na produção de banana, enquanto a resposta ao K foi linear. O nível crítico de K pelo Mehlich-1, na profundidade de 0,20- 0,40 m, foi 0,71 mmol c kg-1.

Learning-induced gene expression in the heads of two Nasonia species that differ in long-term memory formation.

BACKGROUND: Cellular processes underlying memory formation are evolutionary conserved, but natural variation in memory dynamics between animal species or populations is common. The genetic basis of this fascinating phenomenon is poorly understood. Closely related species of Nasonia parasitic wasps differ in long-term memory (LTM) formation: N. vitripennis will form transcription-dependent LTM after a single conditioning trial, whereas the closely-related species N. giraulti will not. Genes that were differentially expressed (DE) after conditioning in N. vitripennis, but not in N. giraulti, were identified as candidate genes that may regulate LTM formation. RESULTS: RNA was collected from heads of both species before and immediately, 4 or 24 hours after conditioning, with 3 replicates per time point. It was sequenced strand-specifically, which allows distinguishing sense from antisense transcripts and improves the quality of expression analyses. We determined conditioning-induced DE compared to naïve controls for both species. These expression patterns were then analysed with GO enrichment analyses for each species and time point, which demonstrated an enrichment of signalling-related genes immediately after conditioning in N. vitripennis only. Analyses of known LTM genes and genes with an opposing expression pattern between the two species revealed additional candidate genes for the difference in LTM formation. These include genes from various signalling cascades, including several members of the Ras and PI3 kinase signalling pathways, and glutamate receptors. Interestingly, several other known LTM genes were exclusively differentially expressed in N. giraulti, which may indicate an LTM-inhibitory mechanism. Among the DE transcripts were also antisense transcripts. Furthermore, antisense transcripts aligning to a number of known memory genes were detected, which may have a role in regulating these genes. CONCLUSION: This study is the first to describe and compare expression patterns of both protein-coding and antisense transcripts, at different time points after conditioning, of two closely related animal species that differ in LTM formation. Several candidate genes that may regulate differences in LTM have been identified. This transcriptome analysis is a valuable resource for future in-depth studies to elucidate the role of candidate genes and antisense transcription in natural variation in LTM formation.

Nutrição mineral e produção de abacaxizeiro 'Pérola', em função das relações K/N na adubação

A adubação do abacaxizeiro deve contemplar a reposição das quantidades de N e K absorvidas pela planta e exportadas pelos frutos e mudas, e o estabelecimento de relações adequadas entre as doses destes nutrientes. Este trabalho objetivou avaliar o efeito de diferentes relações K/N na adubação sobre a nutrição mineral e a produção de abacaxizeiro 'Pérola', em solos de Tabuleiros Costeiros da Paraíba. O experimento foi conduzido em delineamento experimental em blocos casualizados com 13 tratamentos e três repetições. Os tratamentos resultaram da combinação de quatro relações K/N (0,85:1; 1:1; 2:1 e 3:1), duas doses de N (7,2 e 10,8 g planta-1), quatro tratamentos adicionais (relação K/N de 2:1 na maior dose de N, variando-se a fonte, o parcelamento, a época e a forma de aplicação das doses de N e K) e uma testemunha absoluta (sem adubação). O aumento das relações K/N eleva o peso da folha 'D', os teores de K e os valores das relações K/N e K/Mg; reduz os teores de N e não afeta os teores de P, Ca e a relação K/Ca. O aumento das relações K/N não influencia no peso médio, na produtividade e no percentual de frutos das classes I, II e III. A utilização de sulfato de K, o parcelamento das doses de N e K, na forma de KCl, em cinco aplicações, e a aplicação de metade das doses de N e K via foliar na relação 2:1 aumentam o peso da folha 'D', o teor foliar de N e os valores de peso médio, produtividade e percentual de frutos da classe II.

Avaliação nutricional, produção e incidência do mal-do-panamá em bananeira 'Prata-Anã' (AAB) adubada com K, no quarto ciclo

O presente trabalho teve como objetivos estimar a dose de K para obter a máxima eficiência física e econômica, estimar o nível crítico foliar de K e relacionar o equilíbrio nutricional da bananeira 'Prata-Anã' com a incidência de plantas com mal-do-panamá no 4º ciclo. O experimento foi realizado em Latossolo Vermelho-Amarelo (LVA), textura média, na região semiárida do norte de Minas Gerais, Brasil. Os tratamentos consistiram nas aplicações de quatro doses de K (0; 400; 800 e 1.200 kg ha-1 ano de K2O). Estes foram distribuídos em blocos ao acaso, com cinco repetições. A adubação com K promoveu aumento na produção da bananeira no 4º ciclo. A máxima eficiência física (31 Mg ha-1) e a econômica (30,5 Mg ha-1) foram obtidas com as aplicações de 969 e 707 kg ha-1 ano-1 de K2O, respectivamente. Os níveis críticos foliares de K obtidos foram 29,5 e 27,1 g kg-1, para a máxima eficiência física e econômica, respectivamente. O aumento das doses de K proporcionou melhor equilíbrio nutricional à bananeira e reduziu a porcentagem de plantas infestadas com o mal-do-panamá.

Materiaalimallin määrityksestä kylmämuovattujen putkipalkkien K-liitosten FE-analyysissä

Työssä on tutkittu kylmämuovattujen nelikulmaisten putkipalkkien K-liitosten mallinnusta epälineaarisella elementtimenetelmällä. Työn tärkeimpänä tavoitteena on ollut kehittää putkipalkin osien materiaalimalleja siten, että liitosten kestävyyttä voidaan tutkia laboratoriokokeiden ohella luotettavasti myös elementtimenetelmällä. Toisena tavoitteena on ollut tutkia, voidaanko putkipalkkien liitosten mitoitusohjeita turvallisesti soveltaa kylmämuovatuille putkipalkeille, joissa valmistusprosessi aiheuttaa muutoksia materiaaliominaisuuksiin, erityisesti muodonmuutoskykyyn. Työssä tehtyjen laboratoriokokeiden ja elementtianalyysien perusteella elementti-menetelmä on käyttökelpoinen työkalu putkipalkkiliitosten staattista kestävyyttä määritettäessä, kun materiaalimallit on määritetty oikein. Erityisesti liitoksen käyttö-rajatilan mukaisen kestävyyden laskennassa elementtimenetelmällä saadaan hyvin laboratoriokokeita vastaavia tuloksia. Tehdyt laboratoriokokeet osoittavat myös, että Eurocode 3:n mukaisia putkipalkkien liitosten mitoitusohjeita voi turvallisesti käyttää kylmämuovatuille putkipalkeille.