Studies on the enzymatic and transcriptional activity of the dimerization cofactor for hepatocyte nuclear factor 1

The relationship between the enzymatic and the transcriptional activity of the bifunctional protein pterin-4a-carbinolamine dehydratase/dimerization cofactor for hepatocyte nuclear factor 1 (DCoH) has been elucidated by site-directed mutagenesis. DCoH dimers harbor a binding site for hepatocyte nuclear factor 1 (HNF1), two active centers that bind pterins, and a saddle-shaped surface that resembles nucleic acid binding domains. Two domains of the protein have been selectively targeted to determine if a change in one activity affects the other. No strong correlation has been found, supporting the idea that carbinolamine dehydratase activity is not required for HNF1 binding in vitro or transcriptional coactivation in vivo. Double mutations in the active center, however, influence the in vivo transcriptional activity but not HNF1 binding. This finding suggests that some active center residues also are used during transcription, possibly for binding of another (macro)molecule. Several mutations in the saddle led to a surprising increase in transcription, therefore linking this domain to transcriptional regulation as well. The transcriptional function of DCoH therefore is composed of two parts, HNF1 binding and another contributing effect that involves the active site and, indirectly, the saddle.

Role of aromatic residues in stabilization of the [Fe4S4] cluster in high-potential iron proteins (HiPIPs): physical characterization and stability studies of Tyr-19 mutants of Chromatium vinosum HiPIP.

The functional role of residue Tyr-19 of Chromatium vinosum HiPIP has been evaluated by site-directed mutagenesis experiments. The stability of the [Fe4S4] cluster prosthetic center is sensitive to side-chain replacements. Polar residues result in significant instability, while nonpolar residues (especially with aromatic side chains) maintain cluster stability. Two-dimensional NMR data of native and mutant HiPIPs are consistent with a model where Tyr-19 serves to preserve the structural rigidity of the polypeptide backbone, thereby maintaining a hydrophobic barrier for exclusion of water from the cluster cavity. Solvent accessibility results in more facile oxidation of the cluster by atmospheric oxygen, with subsequent rapid hydrolysis of the [Fe4S4]3+ core.

‘La familia ya no es lo que era’: intercultural lesbian relationships in contemporary Spanish cinema

Este artículo considera el papel variable de las mujeres con respecto a la institución del matrimonio y al entorno familiar a través de la representación cinematográfica de relaciones interculturales y de lesbianas. Sostiene que ese tipo de relación constituye un ejemplo de la visibilidad emergente de formas familiares alternativas al modelo heteronormativo. Tomando en cuenta las dimensiones lingüísticas, temporales, y espaciales de estas relaciones, analiza tres películas: Costa Brava: Family Album, A mi madre le gustan las mujeres y Room in Rome. En resumen, el artículo cuestiona la posición del deseo intercultural lésbico dentro de las representaciones cinematográficas de la familia contemporánea en España.

Laboratory effects in beach studies /

Cover title.

Shock tube studies of the effects of sharp-rising, long-duration overpressures on biological systems /

Shock tubes have been used successfully by a number of investigators to study the biological effects of variations in environmental pressures (1,2,3). Recently an unusually versatile laboratory pressurization source became available with the capability of consistently reproducing a wide variety of pressure-time phenomena of durations equal to and well beyond those associated with the detonation of nuclear devices (4). Thus it became possible to supplement costly full-scale field research in blast biology carried out at the Nevada Test Site (5,6) by using an economical yet realistic laboratory tool. In one exploratory study employing pressure pulses of 5 to 10 sec duration wherein the times to max overpressure and the magnitudes of the overpressures were varied, a relatively high tolerance of biological media to pressures well over 150 psi was demonstrated (7). In contrast, the present paper will describe the relatively high biological susceptibility to long duration overpressures in which the pressure rises occurred in single and double fast-rising steps.

Laboratory and full-scale parametric studies of the rolling resistance of radial- and bias-ply heavy service truck tires. Final report.

National Highway Traffic Safety Administration, Office of Heavy Duty Vehicle Research, Washington, D.C.

IVHS institutional issues and case studies. ADVANCE case study. Final report.

Federal Highway Administration, Washington, D.C.

IVHS institutional issues and case studies. TravTek case study. Final report.

Federal Highway Administration, Washington, D.C.

IVHS institutional issues and case studies. TRANSCOM/TRANSMIT case study. Final report.

Federal Highway Administration, Washington, D.C.

IVHS institutional issues and case studies. Westchester commuter central case study. Final report.

Federal Highway Administration, Washington, D.C.

IVHS institutional issues and case studies. Analysis and lessons learned. Final report.

Federal Highway Administration, Washington, D.C.

Studies of freight train engineer performance. Final report.

Federal Railroad Administration, Office of Research and Development, Washington, D.C.

IVHS institutional issues and case studies. Advantage I-75 case study. Final report.

Federal Highway Administration, Washington, D.C.

IVHS institutional issues and case studies. HELP/Crescent case study. Final report.

Federal Highway Administration, Washington, D.C.

Environmental assessment using remotely sensed data : application of remote sensing data to environmental impact studies for transmission line routes /

Mode of access: Internet.