992 resultados para Agrobacterium vitis
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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Pós-graduação em Agronomia (Horticultura) - FCA
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It was evaluated the influence of rootstocks 'IAC 766', 'IAC 572', 'IAC 313', 'IAC 571-6' and 'Ripária do Traviú' and the pruning periods in the duration of phenological stages and in the accumulation of degree-days on grape 'Niagara Rosada' .The experiment was conducted in Louveira, SP. The treatments consisted of five rootstocks and three times of pruning, the experimental design being used in a completely randomized split plot with five replications, and plots represented by rootstocks and the subplots were pruning. After pruning the grapevine, were realized evaluations for phenological stages, using the criterion of Eichhon e Lorenz (1984). ). In three seasons of pruning, based on phenological stages, it was calculated the length of the periods: pruning to budding, pruning to the beginning of the flowering, pruning to the beginning of fruiting, pruning to beginning of ripening of the bunches, pruning the beginning of the harvest. Taking as a basis the cycle length of the vine and the average daytime temperatures, it was calculated the accumulation of degree-days. Data were submitted to ANOVA and Tukey test at 5% significance level. It was obtained in the summer pruning the reduction in duration of the phenological stages of 'Niagara Rosada' grafted on the rootstock 'Ripária Traviú'. The longer duration of the cycle and accumulation of degree-day of 'Niagara Rosada' cultivar were obtained with the rootstocks 'IAC 572' and 'IAC 313' pruning in the winter.
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
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Trials were carried out in Juazeiro, Bahia State, Brazil, aiming to test plant regulators composed by gibberellin, cytokine and auxin effects on chemical quality of Superior Seedless grape berries. The first trial studied the effects of Stimulate (R) (bio regulator) and X-Cyte (R) (cytokine) associated to a new gibberellin formulation (N-Large (R)) and associated to Pro-Gibb (R), which is a product used as source of gibberellin. Products were sprayed at berries development phase (18, 21, 51 and 56 days after spur-pruning). Treatments were: T1: Pro-Gibb (R); T2: Stimulate (R) (Dose 1); T3: Stimulate (R) (Dose 2); T4: Stimulate (R) (Dose 3); T5: Pro-Gibb (R) + X-Cyte (R) (Low Dose - DB); T6: Pro-Gibb (R) + X-Cyte (R) (Intermediate Dose - DM); T7: Pro-Gibb (R) + X-Cyte (R) (High Dose - DA); T8: N-Large (R); T9: N-Large (R) + X-Cyte (R) (DB); T10: N-Large (R) + X-Cyte (R) (DM); T11: N-Large (R) + X-Cyte (R) (DA). The second trial aimed to assess the effect of the new gibberellin formulation (N-Large (R)) associated or not with cytokine (X-Cyte (R)) also sprayed straight over the bunches at berries development phase (17, 55 e 66 days after spur-pruning). Treatments were: T1: Pro-Gibb (R) - blank; T2: N-Large (R) (DB); T3: N-Large (R) (DM); T4: N-Large (R) (DA); T5: N-Large (R) (DB) + X-Cyte (R) (DB); T6: N-Large (R) (DB) + X-Cyte (R) (DM); T7: N-Large (R) (DB) + X-Cyte (R) (DA); T8: N-Large (R) (DM) + X-Cyte (R) (DB); T9: N-Large (R) (DM) + X-Cyte (R) (DM); T10: N-Large (R) (DM) + X-Cyte (R) (DA); T11: N-Large (R) (DA) + X-Cyte (R) (DB); T12: N-Large (R) (DA) + X-Cyte (R) (DM); T13: N-Large (R) (DA) + X-Cyte (R) (DA). Experimental design was random blocks with four repetitions with each repetition/parcel having three useful plants in the same row. At harvest, when bunches average had soluble solids over 15 degrees Brix, berries were collected for soluble solids, pH, titratable acidity analysis as well as (SS/AT) ratio calculation. In both trials, plant regulators evaluated did not provide significant changes on chemical quality of 'Superior Seedless' grape berries. Therefore, the lack of differences on response between the commercially used product (Pro-Gibb (R)) and the other products tested (Stimulate (R), X-Cyte (R) e N-Large (R)) prove the last as promising for the ` Superior Seedless' grape cultivation, leaving a larger range of alternative for grape farmers in the Sao Francisco Valley, Bahia.
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The almost complete absence or misdistribution of water as a natural resource frequently constitute a limiting factor for plant growth and development in the semi-arid northeastern Brazil. In this context, the use of appropriate irrigation techniques is an essential and indispensable factor for proper functioning of the primary and secondary metabolisms in plants. This study aimed to assess the metabolism of the Syrah grapevine in the semi-arid northeastern Brazil, by using three irrigation strategies (controlled deficit irrigation [CDI], deficit irrigation [DI], and full irrigation [FI]). The research was conducted at Embrapa Semiarid (Embrapa-Brazilian Agricultural Research Corporation), Experimental Field of Bebedouro, municipality of Petrolina, state of Pernambuco, Brazil. The statistical design was randomized blocks, with a 3 x 6 factorial scheme. Six samplings were performed throughout the experiment cycle at 54, 60, 73, 87, 101, and 115 days after pruning (DAP). We analyzed the total soluble sugars, reducing sugars, total soluble protein, and invertase activity. Analysis of variance and the F test were performed for all analyzed variables. The means were compared using the Tukey test at 5% significance. At the end of the experiment cycle, DI was found to increase the average acid invertase activity in the plant cell wall. Further, by the end of the experiment cycle, the total soluble sugars and reducing sugars increased in all plants in the three irrigation strategies. Thus, we conclude that it is possible to reduce water consumption in vineyards of the semi-arid northeastern Brazil, without significantly affecting the protein and sugar metabolisms in the plants.
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
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The Coffee Genome Project made available to the scientific community relevant information that made practical the identification and cloning of important genes, as well as the identification of the major sequences involved on their regulation. The aim of the present study was to amplify, clone and sequence coffee promoters with specific expression patterns. For that, coffee ESTs which known expression profiles were employed. First, the promoter regions of coffee genes showing, respectively, fruitspecific and ubiquitous expression were amplified using the Genome Walking strategy. Amplified sequences were then inserted in the pGEM-Teasy vector (Promega) and sequenced. Once completed the sequencing, an expression cassette was constructed using the binary vector pCAMBIA-1381z (Cambia). These expression cassettes were cloned into Agrobacterium tumefaciens, and transgenic tobacco plants were generated aiming the functional characterization of these promoters
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Brazilian wines are appreciated by consumers for having fruity odor and taste which are typical from Vitis labrusca cultivars. The rusticity of these cultivars, the winemaking and the physicochemical properties are some of the key factors to obtain a quality beverage. In this context, the aim of this article was to analyze the quality of three white wines from wineries located in northwest region of São Paulo state by determining physicochemical properties and sensory profile. Data were analyzed by statistical tests and by Principal Component Analysis (PCA). It was possible to observe statistical differences (P<0,05) in most physicochemical properties. Furthermore, the PCA showed that the attributes body and color significantly explained the variance of the first and second component, respectively, higlighting the Vitis vinifera wine. Thus the connection between the physicochemical and sensory properties of white wines was important for characterization of these beverages produced in the northwest of São Paulo.
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This study aimed to investigate the antitumor and cytotoxicity activities of Kielmeyera coriacea and Pyrostegia venusta extracts. Therefore, the hydroalcoholic extracts of P. venusta flowers and K. coriacea leaves were prepared. The extracts were evaporated and the dry extracts were diluted at concentrations of 1.0, 0.1, 0.01 and 0.001 mg/ml for carrying out the bioassays. Artemia salina eggs were incubated in saline solution at 28°C for 24 h. The larvae were treated with different extracts concentrations and the mortality was evaluated after 24 and 48 h. Five discs of potato were placed in Petri dishes and 50 μl of inoculum of Agrobacterium tumefaciens were added to it at 28°C for 24 h incubation. So, 50 μl of the extracts in different concentrations were added. Positive and negative controls were made. The P. venusta and K. coriacea extracts did not show statistically significant acute toxicity. K. coriacea extract showed (mean% of tumor ± standard deviation) 15.30 ± 3.24, 6.34 ± 3.82, 7.57 ± 2.92 and 5.77 ± 2.85 and P. venusta showed 25.82 ± 5.15, 38.40 ± 8.28, 15.75 ± 4.44 and 13.38 ± 7.92, with their concentrations for the antitumor bioassay, and the positive control showed 25.80 ± 6.14. According to the obtained results it was established that the K. coriacea and P. venusta extracts showed antitumor activity but did not show significant cytotoxic activity in A. salina test.
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)