E-1 Monthly Statistical Movement Summary for Entire Iowa Department of Corrections, Iowa Department of Corrections, May 2003

Monthly Statistical Movement Summary for Entire Iowa Department of Corrections

E-1 Monthly Statistical Movement Summary for Entire Iowa Department of Corrections, Iowa Department of Corrections, May 2003

Monthly Statistical Movement Summary for Entire Iowa Department of Corrections

E-1 Monthly Statistical Movement Summary for Entire Iowa Department of Corrections, Iowa Department of Corrections, June 2003

Monthly Statistical Movement Summary for Entire Iowa Department of Corrections

E-1 Monthly Statistical Movement Summary for Entire Iowa Department of Corrections, Iowa Department of Corrections, February 2003

Monthly Statistical Movement Summary for Entire Iowa Department of Corrections

E-1 Monthly Statistical Movement Summary for Entire Iowa Department of Corrections, Iowa Department of Corrections, September 2003

Monthly Statistical Movement Summary for Entire Iowa Department of Corrections

E-1 Monthly Statistical Movement Summary for Entire Iowa Department of Corrections, Iowa Department of Corrections, October 2003

Monthly Statistical Movement Summary for Entire Iowa Department of Corrections

E-1 Monthly Statistical Movement Summary for Entire Iowa Department of Corrections, Iowa Department of Corrections, November 2003

Monthly Statistical Movement Summary for Entire Iowa Department of Corrections

E-1 Monthly Statistical Movement Summary for Entire Iowa Department of Corrections, Iowa Department of Corrections, July 2003

Monthly Statistical Movement Summary for Entire Iowa Department of Corrections

E-1 Monthly Statistical Movement Summary for Entire Iowa Department of Corrections, January 2004

Monthly Statistical Movement Summary for Entire Iowa Department of Corrections

E-1 Monthly Statistical Movement Summary for Entire Iowa Department of Corrections, February 2004

Monthly Statistical Movement Summary for Entire Iowa Department of Corrections

Choice of an adequate promoter for efficient complementation in Saccharomyces cerevisiae: a case study.

Conservation of the function of open reading frames recently identified in fungal genome projects can be assessed by complementation of deletion mutants of putative Saccharomyces cerevisiae orthologs. A parallel complementation assay expressing the homologous wild type S. cerevisiae gene is generally performed as a positive control. However, we and others have found that failure of complementation can occur in this case. We investigated the specific cases of S. cerevisiae TBF1 and TIM54 essential genes. Heterologous complementation with Candida glabrata TBF1 or TIM54 gene was successful using the constitutive promoters TDH3 and TEF. In contrast, homologous complementation with S. cerevisiae TBF1 or TIM54 genes failed using these promoters, and was successful only using the natural promoters of these genes. The reduced growth rate of S. cerevisiae complemented with C. glabrata TBF1 or TIM54 suggested a diminished functionality of the heterologous proteins compared to the homologous proteins. The requirement of the homologous gene for the natural promoter was alleviated for TBF1 when complementation was assayed in the absence of sporulation and germination, and for TIM54 when two regions of the protein presumably responsible for a unique translocation pathway of the TIM54 protein into the mitochondrial membrane were deleted. Our results demonstrate that the use of different promoters may prove necessary to obtain successful complementation, with use of the natural promoter being the best approach for homologous complementation.

E-1 Monthly Statistical Movement Summary for Entire Iowa Department of Corrections, March 2004

Monthly Statistical Movement Summary for Entire Iowa Department of Corrections

E-1 Monthly Statistical Movement Summary for Entire Iowa Department of Corrections, April 2004

Monthly Statistical Movement Summary for Entire Iowa Department of Corrections

E-1 Monthly Statistical Movement Summary for Entire Iowa Department of Corrections, May 2004

Monthly Statistical Movement Summary for Entire Iowa Department of Corrections

Suicide after successful deep brain stimulation for movement disorders.

The authors observed a high rate of suicide (6/140 patients, 4.3%) in a large cohort of patients with movement disorders treated with deep brain stimulation (DBS). Apparent risk factors included a previous history of severe depression and multiple successive DBS surgeries, whereas there was no relationship with the underlying condition, DBS target, electrical parameters, or modifications of treatment. Paradoxically, all patients experienced an excellent motor outcome following the procedure. The authors propose that patients at high risk for suicide should be excluded from DBS surgery.