Painting and Drawing: Mitigating The Space Between the Internal and External

Thesis (Master's)--University of Washington, 2016-06

Kinetic and phylogenetic characterization of an anaerobic dechlorinating microbial community

The reductive dechlorination (RD) of tetrachloroethene (PCE) to vinyl chloride (VC) and, to a lesser extent, to ethene (ETH) by an anaerobic microbial community has been investigated by studying the processes and kinetics of the main physiological components of the consortium. Molecular hydrogen, produced by methanol-utilizing acetogens, was the electron donor for the PCE RD to VC and ETH without forming any appreciable amount of other chlorinated intermediates and in the near absence of methanogenic activity. The microbial community structure of the consortium was investigated by preparing a 1 6S rDNA clone library and by fluorescence in situ hybridization (FISH). The PCR primers used in the clone library allowed the harvest of 16SrDNA from both bacterial and archaeal members in the community. A total of 616 clones were screened by RFLP analysis of the clone inserts followed by the sequencing of RFLP group representatives and phylogenetic analysis. The clone library contained sequences mostly from hitherto undescribed bacteria. No sequences similar to those of the known RD bacteria like 'Dehalococcoides ethenogenes' or Dehalobacter restrictus were found in the clone library, and none of these bacteria was present in the RD consortium according to FISH. Almost all clones fell into six previously described phyla of the bacterial domain, with the majority (56(.)6%) being deep-branching members of the Spirochaetes phylum. Other clones were in the Firmicutes phylum (18(.)5%), the Chloroflexi phylum (16(.)4%), the Bacteroidetes phylum (6(.)3%), the Synergistes genus (11(.)1%) and a lineage that could not be affiliated with existing phyla (11(.)1%). No archaeal clones were found in the clone library. Owing to the phylogenetic novelty of the microbial community with regard to previously cultured microorganisms, no specific microbial component(s) could be hypothetically affiliated with the RD phenotype. The predominance of Spirochaetes in the microbial consortium, the main group revealed by clone library analysis, was confirmed by FISH using a purposely developed probe.

Novel cuticular hydrocarbons from the cane beetle Antitrogus parvulus - 4,6,8,10,16-penta- and 4,6,8,10,16,18-hexamethyldocosanes - Unprecedented anti-anti-anti-stereochemistry in the 4,6,8,10-methyltetrad

[GRAPHICS] The major cuticular hydrocarbons from the cane beetle species Antitrogus parvulus are 4,6,8,10,16-penta- and 4,6,8,10,16,18-hexamethyldocosanes, I and 2, respectively. Stereoisomers of 2,4,6,8-tetramethylundecanal of established relative stereochemistry were derived from 2,4,6-trimethylphenol and were then coupled with appropriate methyl-substituted phosphoranes 62 and 25 to furnish alkenes, which on reduction provided diastereomers of I and 2, respectively. Capillary gas chromatography, mass spectrometry, and high resolution C-13 NMR spectroscopy confirmed 1 as either 84a or 84b and 2 as either 15a or 15b. The novelty of these structures and their relative stereochemistry is briefly related to polyketide assembly.

Characterization of Kudoa monodactyli n. sp (Myxosporea : Multivalvulida) from the muscle of Monodactylus argenteus (Teleostei : Monodactylidae) from Moreton Bay, Queensland, Australia

Kudoa monodactyli n. sp. is described from the somatic musculature of Monodactylus argenteus from several localities in southern Queensland, Australia. This is the first record of a myxozoan parasite from the family Monodactylidae. The spores typically have five polar capsules, making this species similar to the four other five-valved Kudoa species (K. neurophila, K. muscularis, K. shulmani, K. cutanea) that have been described to date. However, morphometric measurements particularly of spore length and width make the species from M. argenteus distinct from the other species. Comparison of the small subunit ribosomal DNA sequence of this species with its congeners for which sequence data are available, provides further evidence of novelty. Kudoa monodactyli n. sp. displays 38 (of 1,554) nucleotide differences compared with rDNA sequence of Kudoa neurophila, which on phylogenetic analysis places these species in clades exclusive of each other. Phylogenetic analyses also provide evidence that the number of valves per spore in this genus is an imperfect indicator of relatedness.

On functionally-fitted Runge-Kutta methods

Functionally-fitted methods are generalizations of collocation techniques to integrate an equation exactly if its solution is a linear combination of a chosen set of basis functions. When these basis functions are chosen as the power functions, we recover classical algebraic collocation methods. This paper shows that functionally-fitted methods can be derived with less restrictive conditions than previously stated in the literature, and that other related results can be derived in a much more elegant way. The novelty in our approach is to fully retain the collocation framework without reverting back into derivations based on cumbersome Taylor series expansions.

Establishing a zone where technology innovation is supported

Learning technologies are now a ubiquitous force in the higher education sector however we continue to pursue more inventive ways to use them for teaching and learning. Many teaching academics that seek to be innovative do not have access to a supportive technology innovation zone. The aim of this study was to investigate the articulated staff development needs of academics involved in a faculty based technology innovation project and create the conditions that would cultivate innovation. The study sought to find out how academics perceived they might best be assisted through their technology innovation process so that participants’ needs were incorporated into planning. A questionnaire was used to elicit background information about the academics’ experience, skills and self diagnosed skill deficits in this context. Participants were also requested to provide information about how they thought they would best acquire the skills given their time and other resource constraints. A modified Delphi Technique was utilised to achieve some consensus on what academics required to support technology innovation. Complemented by an enabling and empowering team based approach, the academics were provided with an innovation zone to achieve significant goals for the project.

Changes in social identity when joining a new group: The role of individual-level and group-level adaptation processes

While classic intergroup theories have specified the processes explaining situational shifts in social identification, the processes whereby social identities change more profoundly and become integrated within the self have to be proposed. To this aim, the present studies investigate the processes by which group members integrate a new social identity as they are joining a new group. Combining a social identity approach and stress and coping models, this research tests if social factors (i.e., needs satisfied by fellow group members, social support), have an impact on the adaptation strategies group members use to deal with the novelty of the situation and to fit into their new group (seeking information & adopting group norms vs. disengaging). These strategies, in turn, should predict changes in level of identification with the new social group over time, as well as enhanced psychological adjustment. These associations are tested among university students over the course of their first academic year (Study 1), and among online gamers joining a newly established online community (Study 2). Path analyses provide support for the hypothesised associations. The results are discussed in light of recent theoretical developments pertaining to intraindividual changes in social identities and their integration in the self.

AMBIGUIDADE COMO INVENTIVIDADE: UM ESTUDO SOBRE O SINCRETISMO RELIGIOSO NA FRONTEIRA ENTRE A ANTROPOLOGIA E A TEOLOGIA

Essa tese é um estudo sobre o problema do sincretismo religioso, quando pensado no diálogo entre a antropologia e a teologia. Para tanto, a pesquisa faz um exercício hermenêutico de ressignificação do conceito, a partir das diversas teorias sobre o sincretismo e seus usos antropológicos e teológicos, buscando subsídios no pensamento de Michel de Certeau e Paul Tillich para tal ressignificação. Assim, a perspectiva certeauniana de uma reflexão heterológica da cultura e a noção de demônico na teologia e filosofia do sentido de Tillich fundamentam a percepção do sincretismo como uma teoria da mediação entre religião e cultura. Isso significa que o sincretismo opera uma relação dialética com o seu pólo denominado, nessa tese, de diacretismo , tornando a dinâmica cultural e religiosa um espaço inventivo, posto que manifesta aspectos ambíguos de criações de sentido positivas (experiência de aproximação sincrética) e criações de sentido distorcidas (experiência de fragmentação diacrética ), essência da relação com o sagrado, vivida culturalmente. A cultura, entendida como espaço para a vivência do religioso, exprime-se em uma profunda relação entre táticas e estratégias, denotando a ambigüidade anteriormente afirmada, quando reconhece que os atores sociais em interação, mesmo que marcados por lugares próprios estrategicamente estabelecidos, enquanto lugares de poder, não inibem a formação de ações táticas que subervertem inventivamente esses mesmos lugares, dando a devida dinâmica cultural. A tese analisa, também, como estudo de caso, as implicações dessa compreensão de sincretismo para a interpretação da experiência religiosa de grupos de indígenas Guarani e Kaiowá, na Terra Indígena de Dourados /MS, na fronteira entre tradição e tradução operada pelos indígenas, a partir do Projeto da Igreja Indígena Presbiteriana (IIP), ressignificando sua alteridade religiosa na interface com os múltiplos cristianismos presentes nas aldeias, afirmando a possibilidade de um teko retã ( jeito de ser plural, múltiplo ) religioso, a partir das relações sincréticas e diacréticas propostas.

DISCURSOS SOBRE A PRÁTICA: O PROCESSO DE FORMAÇÃO DO PROFESSOR DO ENSINO SUPERIOR SEM A FORMAÇÃO PEDAGÓGICA

Esta pesquisa buscou analisar como os professores universitários formados bacharéis e não possuidores da formação pedagógica, interpretam suas práticas docentes e o seu percurso formador. O objetivo foi identificar as representações das práticas destes professores e o impacto do habitus e do campo no seu processo formativo. Foi considerado inicialmente que estes profissionais, que ministram aulas de disciplinas técnicas, não apresentam formação específica (pedagógica) para a condução de tais aulas, porém podem possuir práticas docentes inventivas que foram apreendidas ao longo de sua trajetória acadêmica e profissional e, que por meio delas, mediam o aprendizado. A opção metodológica para o desenvolvimento deste trabalho foi o de realização de pesquisa qualitativa por meio de aplicação de questionário de perfil e entrevista semi-estruturada com os professores sem a formação pedagógica de duas Instituições de Ensino Superior. Estas ferramentas objetivaram captar a representação de um grupo de professores da área de Humanas e de Exatas sobre suas práticas pedagógicas, de sua formação docente e trajetória profissional. O referencial teórico apresenta autores que problematizam as questões relacionadas à formação docente no âmbito do Ensino Superior com destaque para: Cunha (2007); Perrenoud (2002), Tardif (2002), Pimenta e Anastasiou (2002) e Nóvoa (1997). Para a análise das entrevistas, com a perspectiva de compreender a representação e as práticas docentes, foi utilizada parte do corpo teórico desenvolvido por Pierre Bourdieu e, ainda, a discussão sobre a visão instrumental da Didática e da formação pedagógica. O estudo possibilitou demonstrar que os professores do Ensino Superior que participaram desta pesquisa utilizam um conjunto de técnicas, práticas, conhecimentos e formas de conduta que não aprenderam em cursos específicos de formação de docentes. Seu desenvolvimento como professores ocorre, em grande medida, a partir de seu passado escolar e da sua convivência profissional.

Tracking of non-stationary time-series using resource allocating RBF networks

In this paper, we discuss some practical implications for implementing adaptable network algorithms applied to non-stationary time series problems. Using electricity load data and training with the extended Kalman filter, we demonstrate that the dynamic model-order increment procedure of the resource allocating RBF network (RAN) is highly sensitive to the parameters of the novelty criterion. We investigate the use of system noise and forgetting factors for increasing the plasticity of the Kalman filter training algorithm, and discuss the consequences for on-line model order selection. We also find that a recently-proposed alternative novelty criterion, found to be more robust in stationary environments, does not fare so well in the non-stationary case due to the need for filter adaptability during training.

A generative probabilistic oriented wavelet model for texture segmentation

This Letter addresses image segmentation via a generative model approach. A Bayesian network (BNT) in the space of dyadic wavelet transform coefficients is introduced to model texture images. The model is similar to a Hidden Markov model (HMM), but with non-stationary transitive conditional probability distributions. It is composed of discrete hidden variables and observable Gaussian outputs for wavelet coefficients. In particular, the Gabor wavelet transform is considered. The introduced model is compared with the simplest joint Gaussian probabilistic model for Gabor wavelet coefficients for several textures from the Brodatz album [1]. The comparison is based on cross-validation and includes probabilistic model ensembles instead of single models. In addition, the robustness of the models to cope with additive Gaussian noise is investigated. We further study the feasibility of the introduced generative model for image segmentation in the novelty detection framework [2]. Two examples are considered: (i) sea surface pollution detection from intensity images and (ii) image segmentation of the still images with varying illumination across the scene.

Envisioning innovation : the communication of technological change through graphic representation

Graphic depiction is an established method for academics to present concepts about theories of innovation. These expressions have been adopted by policy-makers, the media and businesses. However, there has been little research on the extent of their usage or effectiveness ex-academia. In addition, innovation theorists have ignored this area of study, despite the communication of information about innovation being acknowledged as a major determinant of success for corporate enterprise. The thesis explores some major themes in the theories of innovation and compares how graphics are used to represent them. The thesis examines the contribution of visual sociology and graphic theory to an investigation of a sample of graphics. The methodological focus is a modified content analysis. The following expressions are explored: check lists, matrices, maps and mapping in the management of innovation; models, flow charts, organisational charts and networks in the innovation process; and curves and cycles in the representation of performance and progress. The main conclusion is that academia is leading the way in usage as well as novelty. The graphic message is switching from prescription to description. The computerisation of graphics has created a major role for the information designer. It is recommended that use of the graphic representation of innovation should be increased in all domains, though it is conceded that its content and execution need to improve, too. Education of graphic 'producers', 'intermediaries' and 'consumers' will play a part in this, as will greater exploration of diversity, novelty and convention. Work has begun to tackle this and suggestions for future research are made.

Outlier detection with partial information:Application to emergency mapping

This paper, addresses the problem of novelty detection in the case that the observed data is a mixture of a known 'background' process contaminated with an unknown other process, which generates the outliers, or novel observations. The framework we describe here is quite general, employing univariate classification with incomplete information, based on knowledge of the distribution (the 'probability density function', 'pdf') of the data generated by the 'background' process. The relative proportion of this 'background' component (the 'prior' 'background' 'probability), the 'pdf' and the 'prior' probabilities of all other components are all assumed unknown. The main contribution is a new classification scheme that identifies the maximum proportion of observed data following the known 'background' distribution. The method exploits the Kolmogorov-Smirnov test to estimate the proportions, and afterwards data are Bayes optimally separated. Results, demonstrated with synthetic data, show that this approach can produce more reliable results than a standard novelty detection scheme. The classification algorithm is then applied to the problem of identifying outliers in the SIC2004 data set, in order to detect the radioactive release simulated in the 'oker' data set. We propose this method as a reliable means of novelty detection in the emergency situation which can also be used to identify outliers prior to the application of a more general automatic mapping algorithm. © Springer-Verlag 2007.

The knowledge competitiveness of regional economies: conceptualisation and measurement

Whereas the competitive advantage of firms can arise from size and position within their industry as well as physical assets, the pattern of competition in advanced economies has increasingly come to favour those firms that can mobilise knowledge and technological skills to create novelty in their products. At the same time, regions are attracting growing attention as an economic unit of analysis, with firms increasingly locating their functions in select regions within the global space. This article introduces the concept of knowledge competitiveness, defined as an economy’s knowledge capacity, capability and sustainability, and the extent to which this knowledge is translated into economic value and transferred into the wealth of the citizens. The article discusses the way in which the knowledge competitiveness of regions is measured and further introduces the World Knowledge Competitiveness Index, which is the first composite and relative measure of the knowledge competitiveness of the globe’s best performing regions.

Neural network control of a gas turbine

In this paper we explore the practical use of neural networks for controlling complex non-linear systems. The system used to demonstrate this approach is a simulation of a gas turbine engine typical of those used to power commercial aircraft. The novelty of the work lies in the requirement for multiple controllers which are used to maintain system variables in safe operating regions as well as governing the engine thrust.