A role for histone-like protein H1 (H-NS) in the regulation of hemolysin expression by Serratia marcescens

The histone-like protein H1 (H-NS) is an abundant structural component of the bacterial nucleoid and influences many cellular processes including recombination, transcription and transposition. Mutations in the hns gene encoding H-NS are highly pleiotropic, affecting the expression of many unrelated genes. We have studied the role of H-NS on the regulation of hemolysin gene expression in Serratia marcescens. The Escherichia coli hns mutant carrying S. marcescens hemolysin genes on a plasmid constructed by ligation of the 3.2-kb HindIII-SacI fragment of pR02 into pBluescriptIIKS, showed a high level of expression of this hemolytic factor. To determine the osmoregulation of wild-type and hns defective mutants the cells were grown to mid-logarithmic phase in LB medium with 0.06 or 0.3 M NaCl containing ampicillin and kanamycin, whereas to analyze the effect of pH on hemolysin expression, the cells were grown to late-logarithmic phase in LB medium buffered with 0.1 M Tris-HCl, pH 4.5 to 8.0. To assay growth phase-related hemolysin production, bacterial cells were grown in LB medium supplemented with ampicillin and kanamycin. The expression of S. marcescens hemolysin genes in wild-type E. coli and in an hns-defective derivative at different pH and during different growth phases indicated that, in the absence of H-NS, the expression of hemolysin did not vary with pH changes or growth phases. Furthermore, the data suggest that H-NS may play an important role in the regulation of hemolysin expression in S. marcescens and its effect may be due to changes in DNA topology influencing transcription and thus the amount of hemolysin expression. Implications for the mechanism by which H-NS influences gene expression are discussed.

La Suede, suivant observations de mess.rs de l'Academie Royale des Sciences, etc. Augmentées de nouveau.

Privilegiomerkintä: Avec privilege.

A new & accurate map of the North Pole with all the countries hitherto discovered situated near or adjacent to it as well as some others more remote. Drawn from the latest and best authorities and regulated by astronom.l observat.ns by Eman. Bowen.

0-meridiaani: Lontoo. - Koordinaattiasteikko: N90°-55°[50°].

Implementatiоn оf Sustainability-Оriented Innоvatiоns in Business - Studying Оrganizatiоnal and External Capabilities: Evidence frоm Pоland

The cоncept оf sustainability-оriented innоvatiоn is recent and still under researched. The aim оf the Thesis is tо cоntribute tо the field and investigate hоw dо cоmpanies оperating in Pоland apply sustainability-оriented innоvatiоn (SОI) tо their cоre business activities, what are the differences between variоus business fоrms оf оrganizatiоn in terms оf SОI, and what type оf capabilities facilitate implementatiоn оf SОI. Given early stage оf empirical research оn sustainability-оriented innоvatiоn, an explоratоry-descriptive case study research strategy was taken applying qualitative methоds. 6 interviews with managers and CEОs оf 4 cоmpanies lоcated in Warsaw were cоnducted. In additiоn, twо academic expert panels with specialists frоm University оf Lоdz and Lappeenranta University оf Technоlоgy were carried оut in оrder tо suppоrt the findings. The study fоund оut that in case оf cоmpanies which purpоse is tо create pоsitive impact and develоp sustainable prоducts оr services by using innоvative apprоaches, SОI activities are embedded in оrganizatiоnal culture and prоcess sо that it is difficult tо differentiate between main business activities and SОI. In the оther twо cases SОI practices were in line with cоre business activities thus reflected the main оperatiоns and were determined as a part оf CSR strategy. Activities are industry specific and are cоntingent upоn resоurces and capabilities pоssessed. Amоng list оf success factоrs management suppоrt, CEО’s persоnal values, dedicated and mоtivated team, investments in research and develоpment, оrganizatiоnal culture, nоn-hierarchical cоmmunicatiоns channels, empоwerment оf emplоyees, prоvisiоn оf time and space fоr failures were identified as key оrganizatiоnal capabilities facilitating integratiоn оf SОI practices. Whereas market demand, NGОs’ pressure, regulatiоns enfоrced, access tо external funding, netwоrking and cооperating present external оr cоllabоrative capabilities suppоrting implementatiоn оf sustainability оriented innоvatiоn in cоmpanies. SОI takes a systemic apprоach that drives the transfоrmatiоn tо becоme sustainable business embedding and integrating sоcial, envirоnmental and ecоnоmic value creatiоn tоgether.

Meletemata philosophica varii argumenti, cons ampl. fac. phil Aboëns. auctore Johanne Ström, phil. mag. v.d.m & apolog. sch. triv. Björneb. Respondente Dieterico Johanne Lohman, stip. reg. Bor. Finl. die XXV Junii MDLLCXXIII, loco horisque a. m. solitis, publice examinanda

Invokaatio: D.D.

Catalogus librorum & instrumentorum chirurgicorum b. m. viri Josephi Pippingsköld, m. d. profess. chirurg. [et] art. obstetr. ad Imp. Acad. Aboëns. nec non imp. coll. med. assessoris, sub hasta d. Oct. XXX & sqq. a. MDCCCXVI

Julkaisuvuosi nimekkeestä.

Förteckning öfver framlidne collegii-assessoren, landt-räntmästaren och riddaren S. J. Linséns boksamling, som kommer att genom offentelig auction försäljas

Imprimatur: S. J. Backman.

Förteckning öfver framlidne lectoren vid Åbo Gymnasium, theologiæ doctorn och Ordens ledamoten Eric Elfgréns efterlemnade böcker, hvilka komma att å auction försäljas

Imprimatur: Nils Abr. Gyldén.

Förteckning öfver framlidne eloquentiae et poeseos professoren m. m. Johan Gabriel Linséns efterlemnade boksamling, som kommer att i Helsingfors genom offentlig auktion försäljas den [ ]

Imprimatur: N. A. Gyldén.

Nós e os gregos

Este artigo responde afirmativamente à questão, vez por outra retomada, da efetiva contribuição que o estudo do pensamento político dos antigos gregos pode trazer ao debate dos problemas com que se defrontam as modernas sociedades democráticas.

"Chegou a hora de darmos a luz a nós mesmas": Situando-nos enquanto mulheres e negras

O artigo, ao analisar manifestações de mulheres negras, busca entender como se constituem cidadãs numa sociedade que discrimina seu grupo e etnia. À indagação sobre o que significa ser mulher e negra, formulada a mulheres militantes do Movimento Negro nos estados do sul do país, mostrou que o configurar-se como mulheres negras implica enfrentar atitudes e posturas discriminatórias, além de exigir combatividade, introspecção, auto-imagem positiva, crítica a relações sociais e propostas para transformá-las. Buscando elucidar sua presença na sociedade, o estudo mostra que lutam para superar a invisibilidade conferida aos descendentes de africanos, bem como para ter suas necessidades atendidas por políticas públicas que resolvam os problemas que os afligem e suprimam as opressões que lhes são impingidas. Assim, lutam por justiça para todos que são marginalizados pela sociedade. O artigo conclui com a afirmativa de que as fontes mais genuínas de conhecimento sobre as mulheres negras são elas mesmas, sendo necessário que estudos que as tomem por temática, considerem seus pontos de vista de mulheres e negras.

Le Nouveau spectateur : correspondance rédigée par Roger Allard

Périodicité : Bimensuel (irrégulier)

Le Nouveau spectateur : correspondance rédigée par Roger Allard

1919 (N1)-1921 (N20).