Structural complexity of the nitrogen source and influence on yeast growth and fermentation

The structural complexity of the nitrogen source strongly affects both biomass and ethanol production by industrial strains of Saccharomyces cerevisiae, during fermentation in media containing glucose or maltose, and supplemented with a nitrogen source varying from a single ammonium salt (ammonium sulfate) to free amino acids (casamino acids) and peptides (peptone). Diauxie was observed at low glucose and maltose concentrations independent of nitrogen supplementation. At high sugar concentrations diauxie was not easily observed. and growth and ethanol production depended on the nature of the nitrogen source. This was different for baking and brewing ale and lager yeast strains. Sugar concentration had a strong effect on the shift from oxido-fermentative to oxidative metabolism. At low sugar concentrations, biomass production was similar under both peptone and casamino acid supplementation. Under casamino acid supplementation, the time for metabolic shift increased with the glucose concentration, together with a decrease in the biomass production. This drastic effect on glucose fermentation resulted in the extinction of the second growth phase, probably due to the loss of cell viability. Ammonium salts always induced poor yeast performance. In general, supplementation with a nitrogen source in the peptide form (peptone) was more positive for yeast metabolism, inducing higher biomass and ethanol production, and preserving yeast viability, in both glucose and maltose media, for baking and brewing ale and lager yeast strains. Determination of amino acid utilization showed that most free and peptide amino acids present, in peptone and casamino acids, were utilized by the yeast, suggesting that the results described in this work were not due to a nutritional status induced by nitrogen limitation.

Growth and survival of pacu Piaractus mesopotamicus (Holmberg, 1887) juveniles reared in ponds or at different initial larviculture periods indoors

Five-day-old pacu larvae (Piaractus mesopotamicus) with average length and weight of 5.96 mm and 0.42 mg, respectively, were reared as follows: in a semi-intensive system with larvae stocked directly into fertilized ponds (IL0)-and an initial intensive larviculture system with larvae maintained in a laboratory for 3 (IL3), 6 (IL6) and 9 (IL9) days, before being transferred to fertilized ponds. During the indoor phase, larvae were fed Artemia nauplii. Intensive-culture survivals were high (95.6%, 86.4% and 83.8% for IL3, IL6 and IL9, respectively) and at the end of the 45-day period, the longer the larvae were kept in the intensive system, the better the juvenile survival in the ponds. IL9 and IL6 survival rates were 54.0% and 45.4%, respectively, significantly higher (P < 0.05) than IL0 (11%) and IL3 presented an intermediate rate (25.3%). Due to the low survival rate of IL0, length and weight were higher (P < 0.05) when compared to IL6 and IL9; and the differences between their survival rates affected size distribution of juveniles among treatments. Treatments, which resulted in high survival (IL6 and IL9), presented a great number of small fish. In contrast, IL0 and IL3 produced many large and extra large individuals. In general, the results indicate that pacu juvenile production by initial intensive larviculture (IL6 and IL9) was the most efficient method. Therefore, further studies should be conducted in order to improve larval growth in the laboratory and handling techniques in both the laboratory and ponds. (C) 2003 Elsevier B.V. B.V. All rights reserved.

Growth and characterization of BaBi2Nb2O9 thin films made by RF-magnetron sputtering

The RF-magnetron sputtering technique has been used to deposit polycrystalline thin films of layered-structured ferroelectric BaBi2Nb2O9 (BBN). The XRD patterns for the films annealed at 700degreesC for 1 hour show the presence of the BBN phase as well as the BaNb2O6 secondary phase. A better crystallization of the BBN phase and an inhibition of the secondary phase is obtained with the increase of temperature. The surface of the prepared films was rather dense and smooth with no cracks. The 300 nm thick BBN thin films exhibited a room-temperature dielectric constant of about 779 with a dissipation factor of 0.09 at a frequency of 100 kHz.

Growth and juvenile development of Uca maracoani Latreille 1802-1803 in laboratory conditions (Crustacea, Decapoda, Brachyura, Ocypodidae).

The life cycle of decapod crustaceans can be classified into three distinct morphological phases: larval, juvenile and adult. Despite its recognized importance, studies of the juvenile phase have been neglected. The present Study aimed to analyze the growth of juveniles from a single population of Uca maracoani under laboratory conditions, and also to describe the morphological differentiation of pleopods in each sex. Megalopae and juvenile crabs or U. maracoani obtained on a Mud beach at Jabaquara, Paraty, on the southern coast of the state of Rio de Janeiro (Brazil), were reared in the laboratory. The specimens were checked daily for molts and deaths. The carapace widths (CW) of intact exuviae and dead individuals were measured under a stereoscopic microscope provided with a micrometer rule. These data allowed the definition of a growth equation as well as the stages related to the beginning of pleopod development, which begins when females reach 3.0 mill CW (6th juvenile developmental stage), similar to the sizes reported for other species of the genus. In males, however, pleopods appear when the crabs reach 3.5 mm CW, equivalent to the 7th developmental stage. This difference may be related to differential growth between sexes. It also may be a consequence of laboratory rearing, or may represent an actual feature of the species.

Effect of foliar and substrate fertilization on lady palm seedling growth and development

Lady palm, [Rhapis excelsa (Thunberg) Henry ex. Rehder] is one of the most cultivated ornamental palms in the world, for use as a vase plant or in shaded landscapes. Because limited information exists on lady palm response to fertilizers, the objective of this study was to evaluate the effect of different types of fertilization and substrates on lady palm seedling growth and development. Three year old lady palms were planted in 8-L pots, filled with a mix of soil, manure, and sand 1:1:1 (v:v:v), placed under a 50% shade, and irrigated with microspray. Treatments were substrate fertilization with 500 g P(2)O(5) and 100 g K(2)O per m(3); fertilization with 1.8 kg of P(2)O(5) (simple superphosphate) per m3; 50 g of nitrogen (N), P(2)O(5), and K(2)O of a granulated fertilizer (10:10:10) per m(3), control (without fertilization), and a foliar fertilization in addition to these treatments using the commercial product Biofert (8:9:9). Treatments were replicated four times in a randomized block design. Each treatment plot consisted of four plants. Data were collected at 140, 170, 200, 230, 260, and 290 days after transplanting (DAT) for plant heights, stem diameter at substrate level, number of leaves, shoots, and canopy, roots fresh and dry matter samples were harvest at 290 days. Foliar fertilization resulted in significantly greater plant height in a 140, 120, 200, and 230 DAT and plant diameter on the 140, 260, and 290 DAT. There was interaction among factors for number of leaves with fertilization based on P(2)O(5) and K(2)O when leaf fertilizer was added that resulted in a greater number of leaves.

Synthesis, Characterization, Anisotropic Growth and Photoluminescence of BaWO4

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Effect of different surfactants on the shape, growth and photoluminescence behavior of MnWO4 crystals synthesized by the microwave-hydrothermal method

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Growth and home range size of the gracile mouse opossum Gracilinanus microtarsus (Marsupialia: Didelphidae) in Brazilian cerrado

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Effects of research and extension on agricultural growth and equity in Brazil.

Studies the effects of investment in research and extension on total agricultural production and on production of individual crops. Some distributional effects are briefly discussed. Due to the lack of data, the first part of the analysis is restricted to the State of Sao Paulo, and the second part covers the entire country.-from Author

The effects of pre-planting refrigeration on bulbs over the development and yield of garlic 'Chines', 'Contestado' and 'Quiteria'

The objective of this research uas to evaluate the effect of five pre-planting refrigeration periods previously to the cloves planting (0,10,20, 30 and 40 days at a temperature of 4.0 ± 1° C and three garlic cultivars ('Chines', 'Contestado' and 'Quiteria') uere used. According to the obtained results it was observed that the best preplanting refrigeration period was dependent upon the cultivar behavior. For the 'Chines' well adapted to the region, there was no effect of the pre-planting refrigeration period up to 30 days after treatment on the plant height, bulb weight, and total bulb production. 'Contestado' the best pre-planting refrigeration period was 40 days however, there was no statistical difference among 30 and 40, days for the 'Quiteria'. For both cultivars pre-planting refrigeration periods under 20 days, there was no clove's formation.

Effects of uracil calculi on cell growth and apoptosis in the BBN- initiated Wistar rat urinary bladder mucosa

The different potential of initiated and non-initiated urinary bladder mucosa (UBM) to develop neoplasia was quantitatively evaluated in the male Wistar rat. Initiation of carcinogenesis was accomplished with N-butyl-N-(4- hydroxybutyl)-nitrosamine (BBN). Stimuli for cell proliferation and apoptosis were obtained by exposure followed by withdrawal of 3% Uracil in the diet. The proliferation index (PI) was estimated in UBM immunostained for the proliferating nuclear cell antigen (PCNA). The apoptotic index (AI) and the density of papillary/nodular hyperplasia (PNH) were estimated in hematoxilin- eosin stained sections. PNH was the main proliferative response to the mechanical irritation by uracil, irrespective of previous initiation with BBN. Uracil exposure induced higher PI and PNH density in the initiated rats. After uracil withdrawal, there was a significant increase of the AI in both uracil-treated groups, which correlated well to the respective PNH density. However, at the end of the experiment, PNH incidence and density were significantly higher in the BBN-initiated mucosa, which also presented 18% incidence of papillomas and 27% of carcinomas. Therefore, under prolonged uracil calculi trauma, the UBM of BBN-initiated Wistar rats gives rise to epithelial proliferative lesions that progress to neoplasia through acquired resistance to apoptosis.

Cotton growth and boron distribution in the plant as affected by a temporary deficiency of boron

The knowledge of nutrient mobility is an important tool to define the best fertilizer management and diagnosis techniques. Patterns of boron (B) mobility in plants have been reviewed, but there is very little information on B distribution and mobility in cotton. An experiment was conducted to study plant growth and B distribution in cotton when the nutrient was applied in the nutrient solution or to the leaves, and when a temporary deficiency was imposed. Cotton (Gossypium hirsutum, Latifolia, cv. IAC 22) was grown in nutrient solutions where B was omitted or not for 15 days. Boron was applied to young or mature cotton leaves in some of the minus B treatments. Root growth decreased when the plants were transferred to B solutions, but there was a full recovery when B was replaced in the nutrient medium. Boron deficiency, even when temporary, reduced cotton shoot dry matter yields, plant height and flower and fruit set, and these could not be prevented by foliar application of B. Because of decreased dry matter production, leaves of deficient cotton plants actually showed higher B concentrations than non deficient leaves. This would be misleading when a mature leaf is sampled for diagnosis. If there is any B mobility in cotton phloem, it is very low.

Effects of food quality on growth and biochemical composition of a calanoid copepod, Argyrodiaptomus furcatus, and its importance as a natural food source for larvae of two tropical fishes

Growth, reproduction and biochemical composition were analyzed for the copepod Argyrodiaptomus furcatus fed on the alga Ankistrodesmus gracilis grown in different media. The ingestion of this copepod by larvae of two species of tropical fishes was also evaluated. The mean peak density of the copepod population was 1369 individuals 1-1 for all four diets used, and the highest was 1387 individuals 1-1 on diet ARV (algae + ration + vitamins). A small copepod, A. furcatus tends to have a short life span. The smallest females did not attain maturity in the shortest time on all diets used. Food quality may play a major role in the dynamics of the biochemical composition of this copepod. Argyrodiaptomus furcatus was a more important food item for larvae of tambaqui (Colossoma macropomum) than of pacu (Piaractus mesopotamicus). However, it made up a large part of the gut contents of larvae of both species.