Modelo estrutural do maciço rochoso do Depósito de Cobre e Ouro da Mina da Chapada, Alto Horizonte, GO

The Cu-Au mine of Chapada is located in the municipality of Alto Horizonte, in the northwestern portion of Goiás state and is inserted in the geological context of the Brasilia Belt, specifically the Mara Rosa Magmatic Arc, which hosts important deposits of Au and Cu-Au. The rocks found in the study area belong mainly to the Volcano-Sedimentary Sequence of Mara Rosa and are composed of basic to acidic metavolcanic rocks, psammiticpellitic metasedimentary rocks, chemical rocks and also hydrothermal products. Late intrusions occur and are represented by pegmatitic dikes and tonalitic bodies. The ore deposit of the Chapada mine is formed predominantly by the chalcopyrite-pyritemagnetite association, where pyrite is the most abundant mineral. Through the structural mapping of the mining fronts, it was able to recognize three deformational phases (Dn, Dn +1, Dn +2). During the Dn phase, isoclinal recumbent folds were formed, in association with amphibolites facies metamorphism. Later, in phase Dn +1, there was formation of drag folds and intrafolial folds in association with retrograde metamorphism in the greenschist facies. The deformational phase Dn +2, in its turn, was responsible for late symmetrical folding of the foliation, with NS and EW axes, resulting in an interference pattern of the dome-and-basin type

Levantamento estrutural da porção norte do Greenstone Belt do rio Itapicuru e dos Alvos Encantado e C1W, Bahia

The study area is located in the geological context of the northern portion of the Rio Itapicuru Greenstone Belt (GBRI), located northeast of the São Francisco Craton, Bahia State. The GBRI is known for its great economic potential for gold mineralization, looking south at Fazenda Brasileiro Mine and North Mine Maria Preta, is situated in the study área. The structural pattern of the northern portion of the Greenstone, theme of this work, is of great importance to understand the mineralization present. To characterize the structural model three geologic sections (scale 1:25000) were made with NW-SE and EW directions and detailing two mineralized targets, Encantado, located northwest of the area, near the city of Nordestina, and C1W, located near Itapicuru River, west of the Maria Preta mine. Thus, field work and petrographic analysis were conducted, that allow to indicate the existence of two structural domains characterized by the existence of a main foliation, low angle dipping main foliation and another with high dip angle, both with NW, moreover, folds occur in the area Dn-1 and post-Dn folds. Dn-1 folds are observed less frequently but they occur mainly forming an oblique foliation and the Sn is present in porphyroblasts observed in petrographic analysis, whereas in relation to the folds post Dn, there are more than one type of folding in area, which differ by the orientation of their axis, a post Dn NS and EW Dn another post. Moreover, two areas of metamorphic green schist (biotite zone) and medium amphibolite, which are directly related to the structural domains found

Estudo da interação não-nativa no enovelamento de proteínas

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Transconversações queer: sussurros e gemidos lusófonos Quatro cadelas mirando a(s) Psicologia(s)

To follow the cartographies’ path throughout pernicious (dis)courses of the capitalized Psychology. Com/positions both around desires and binary desires raised from binary norms, followed by (un)folding the beds, the “family”, the clinical thought and practice, the research, the geni(t)ality. Profane writing. Epistemic micro-rebellion promised and incorporated by the ass, because in the beginning it was and it (also) is the ass. A distorted and a trans-barked way of writing by four female dogs looking at Psychology. To (d)enounce the ménage that psychologists, supported by a history without history, intent to build over their empty beds which are blemished by modern Truth(s). Towards a minor psychology, this is a schizo-queer-analytic manifesto on desires-pleasures-senses within the lusophone transcontemporaneity. In the absence of a conclusion, we propose rather the provocative state that only a bitchy-psychology-to-come could edify the visibility of the invisible human. Otherwise, we believe it is in the promising barks that Psychology can be withdrawn from its betraying and betrayed contention of multitudes

Papel da hidrofobicidade na evolução de proteínas

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Cônicas: Situações Didáticas para o ensino médio

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Expression, purification and characterization of cold shock protein A of Corynebacterium pseudotuberculosis

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Nonlocal effects in the 8Be breakup

A collective Hamiltonian for a two alpha particles aggregate, which describes the 8Be nucleus, encompassing a collective potential and an inertia function of that system, is obtained and analyzed through the use of a technique - derived from an approach of the generator coordinate method (GCM) - which allows for the extraction of collective information. The nucleon-nucleon interaction considered here is the one proposed by Volkov plus the Coulomb repulsion. It is shown that nonlocal effects appear in those collective functions describing the spontaneously occurring breakup process. Furthermore, the result for the inertia function stands for a microscopically generated evidence supporting a double-folding-based model of the real part of the nucleus-nucleus nonlocal interaction recently proposed.

Origami e kirigami: arte e cultura como recurso lúdico e educativo

We can know a people through their cultural and artistic assets. One of the many aspects of Japanese culture is origami, a fusion of the verb “oru”, which means folding, with the word “kami” meaning paper. In this communication, we describe the course “Origami and Kirigami: art and culture as a recreational and educational resource”. The course aimed to present these two oriental techniques based on paper and its potential as a source of entertainment and education, at the same time seeking to introduce cultural aspects of these arts of folding and/or cutting paper. This practice is more common than we realize, and is present in our day-to-day life when we perform actions such as folding clothes and papers, and making packages, amongst others. However, few are aware of the benefits that this folding brings to the fields of Arts, Mathematics, and Science, besides its recreational characteristics. Kirigami is a mixed technique that in addition to using folds in the paper (as in origami) also uses cuts (“kiru” – meaning, “cut”). It can be performed with heavier paper than origami, and by introducing some cuts, the paper can be folded to form the desired shape. It is a simple technique, with impressive results. We conducted eight weekly meetings, each lasting four hours, totaling 32 hours of coursework. In addition to the classes, a visit was made to the Okinawa Club in Bauru (São Paulo), where it was possible for the students of the course and the elderly group (fujinkai) of origamists of the club to exchange experiences. Finally, an exhibition was organized to display the artifacts produced by the course participants and disseminate the work of the students.

Origami científico: a linguagem das dobraduras dentro do design contemporâneo

After crossing several generations and countries, broader functions are being incorporated to origami than simply making art objects: the fold initiates a series of new buildings within the design and creation. Under the new repre- sentations and performances that the art of paper folding keeps, this article aims to investigate how the origami is configured nowadays and how it behaves while contemporary language applied to projective processes of diverse natures. To better understand the actual origami, relations are used inside branch of math, conceptual and projectual. Relating theorists, artists, designers, etc., can point out the relevance of each of these areas in the configuration of cientific and projectual origami. The modularity, the collective, the conscious build, the contemporary and the inno- vation are relationships intrinsic to the praxis projectual that shape a landscape adjacent and subordinate to the main structure governing. It bets on the essence of origami as a tangible functionality for creative thinking.

Origami contemporâneo: um estudo sobre a arte em dobra e sua mutabilidade prática

This article aims to seek new forms of creative and artistic expression origami nowadays. After crossing many generations and countries, we noted that the functions of origami are much broader than simply making folding paper: the folding has promoted a number of new buildings within the hybrid design and creativity. Modularity, the collective, the build conscious, contemporary and innovation are intrinsic relations to artistic praxis that configure an adjacent scenario and subordinate to the main structure that govern this research project. We believe in the essence of origami as a tangible functionality for creative thinking.

Development of an Electrochemical Insulin Sensor Based on a High Affinity DNA Sequence Found in the Insulin-linked Polymorphic Region

The detection of pertinent biomarkers has the potential provide an early indication of disease progression before considerable damage has been incurred. A decrease in an individual’s sensitivity to insulin, which may be quantified as the ratio of insulin to glucose in the blood after a glucose pulse, has recently been reported as an early predictor of insulin-dependent diabetes mellitus. Routine measurement of insulin levels is therefore desirable in the care of diabetes-prone individuals. A rapid, simple, and reagentless method for insulin detection would allow for wide-spread screenings that provide earlier signs of diabetes onset. The aim of this thesis is to develop a folding-base electrochemical sensor for the detection of insulin. The sensor described herein consists of a DNA probe immobilized on a gold disc electrode via an alkanethiol linker and embedded in an alkanethiol self-assembled monolayer. The probe is labeled with a redox reporter, which readily transfers electrons to the gold electrode in the absence of insulin. In the presence of insulin, electron transfer is inhibited, presumably due to a binding-induced conformational or dynamic change in the DNA probe that significantly alters the electron-tunneling pathway. A 28-base segment of the insulin-linked polymorphic region that has been reported to bind insulin with high affinity serves as the capture element of the DNA probe. Three probe constructs that vary in their secondary structure and position of the redox label are evaluated for their utility as insulin-sensing elements on the electrochemical platform. The effects of probe modification on secondary structure are also evaluated using circular dichroism spectroscopy.

Functional and structural studies of the disulfide isomerase DsbC from the plant pathogen Xylella fastidiosa reveals a redox-dependent oligomeric modulation in vitro

Xylella fastidiosa is a Gram-negative bacterium that grows as a biofilm inside the xylem vessels of susceptible plants and causes several economically relevant crop diseases. In the present study, we report the functional and low-resolution structural characterization of the X. fastidiosa disulfide isomerase DsbC (XfDsbC). DsbC is part of the disulfide bond reduction/isomerization pathway in the bacterial periplasm and plays an important role in oxidative protein folding. In the present study, we demonstrate the presence of XfDsbC during different stages of X. fastidiosa biofilm development. XfDsbC was not detected during X. fastidiosa planktonic growth; however, after administering a sublethal copper shock, we observed an overexpression of XfDsbC that also occurred during planktonic growth. These results suggest that X. fastidiosa can use XfDsbC in vivo under oxidative stress conditions similar to those induced by copper. In addition, using dynamic light scattering and small-angle X-ray scattering, we observed that the oligomeric state of XfDsbC in vitro may be dependent on the redox environment. Under reducing conditions, XfDsbC is present as a dimer, whereas a putative tetrameric form was observed under nonreducing conditions. Taken together, our findings demonstrate the overexpression of XfDsbC during biofilm formation and provide the first structural model of a bacterial disulfide isomerase in solution. Structured digital abstract XfDsbC and XfDsbC bind by x ray scattering (View Interaction: 1, 2) XfDsbC and XfDsbC bind by molecular sieving (View interaction) XfDsbC and XfDsbC bind by comigration in non denaturing gel electrophoresis (View interaction) XfDsbC and XfDsbC bind by cross-linking study (View Interaction: 1, 2) XfDsbC and XfDsbC bind by dynamic light scattering (View Interaction: 1, 2)

Ordovician klippen structures of the Sierra de Umango: New insights on Tectonic evolution of the Western Sierras Pampeanas, Argentina

The basement rock of the Pampean flat-slab (Sierras Pampeanas) in the Central Andes was uplifted and rotated in the Cenozoic era. The Western Sierras Pampeanas are characterised by meta-igneous rocks of Grenvillian Mesoproterozoic age and metasedimentary units metamorphosed in the Ordovician period. These rocks, known as the northern Cuyania composite terrane, were derived from Laurentia and accreted toward Western Gondwana during the Early Paleozoic. The Sierra de Umango is the westernmost range of the Western Sierras Pampeanas.This range is bounded by the Devonian sedimentary rocks of the Precordillera on the western side and Tertiary rocks from the Sierra de Maz and Sierra del Espinal on the eastern side and contains igneous and sedimentary rocks outcroppings from the Famatina System on the far eastern side. The Sierra de Umango evolved during a period of polyphase tectonic activity, including an Ordovician collisional event, a Devonian compressional deformation, Late Paleozoic and Mesozoic extensional faulting and sedimentation (Paganzo and Ischigualasto basins) and compressional deformation of the Andean foreland during the Cenozoic. A Nappe System and an important shear zone, La Puntilla-La Falda Shear Zone (PFSZ), characterise the Ordovician collisional event, which was related to the accretion of Cuyania Terrane to the proto-Andean margin of Gondwana. Three continuous deformational phases are recognised for this event: the D1 phase is distinguished by relics of 51 preserved as internal foliation within interkinematic staurolite por-phyroblasts and likely represents the progressive metamorphic stage; the D2 phase exhibits P-T conditions close to the metamorphic peak that were recorded in an 52 transposition or a mylonitic foliation and determine the main structure of Umango; and the D3 phase is described as a set of tight to recumbent folds with S3 axial plane foliation, often related to thrust faults, indicating the retrogressive metamorphic stage. The Nappe System shows a top-to-the S/SW sense direction of movement, and the PFSZ served as a right lateral ramp in the exhumation process. This structural pattern is indicative of an oblique collision, with the Cuyania Terrane subducting under the proto-Andean margin of Gondwana in the NE direction. This continental subduction and exhumation lasted at least 30 million years, nearly the entire Ordovician period, and produced metamorphic conditions of upper amphibolite-to-granulite facies in medium- to high-pressure regimes. At least two later events deformed the earlier structures: D4 and D5 deformational phases. The D4 deformational phase corresponds to upright folding, with wavelengths of approximately 10 km and a general N-S orientation. These folds modified the S2 surface in an approximately cylindrical manner and are associated with exposed, discrete shear zones in the Silurian Guandacolinos Granite. The cylindrical pattern and subhorizontal axis of the D4 folds indicates that the S2 surface was originally flat-lying. The D4 folds are responsible for preserving the basement unit Juchi Orthogneiss synformal klippen. This deformation corresponds to the Chanica Tectonic during the interval between the Devonian and Carboniferous periods. The D5 deformational phase comprehends cuspate-lobate shaped open plunging folds with E W high-angle axes (D5 folds) and sub-vertical spaced cleavage. The D5 folds and related spaced cleavage deformed the previous structures and could be associated with uplifting during the Andean Cycle. (C) 2012 Elsevier Ltd. All rights reserved.

Proteomic analysis of Porphyromonas gingivalis exposed to nicotine and cotinine

Cogo K, de Andrade A, Labate CA, Bergamaschi CC, Berto LA, Franco GCN, Goncalves RB, Groppo FC. Proteomic analysis of Porphyromonas gingivalis exposed to nicotine and cotinine. J Periodont Res 2012; 47: 766775. (c) 2012 John Wiley & Sons A/S Background and Objective: Smokers are more predisposed than nonsmokers to infection with Porphyromonas gingivalis, one of the most important pathogens involved in the onset and development of periodontitis. It has also been observed that tobacco, and tobacco derivatives such as nicotine and cotinine, can induce modifications to P. gingivalis virulence. However, the effect of the major compounds derived from cigarettes on expression of protein by P.gingivalis is poorly understood. Therefore, this study aimed to evaluate and compare the effects of nicotine and cotinine on the P.gingivalis proteomic profile. Material and Methods: Total proteins of P gingivalis exposed to nicotine and cotinine were extracted and separated by two-dimensional electrophoresis. Proteins differentially expressed were successfully identified through liquid chromatography-mass spectrometry and primary sequence databases using MASCOT search engine, and gene ontology was carried out using DAVID tools. Results: Of the approximately 410 protein spots that were reproducibly detected on each gel, 23 were differentially expressed in at least one of the treatments. A particular increase was seen in proteins involved in metabolism, virulence and acquisition of peptides, protein synthesis and folding, transcription and oxidative stress. Few proteins showed significant decreases in expression; those that did are involved in cell envelope biosynthesis and proteolysis and also in metabolism. Conclusion: Our results characterized the changes in the proteome of P.gingivalis following exposure to nicotine and cotinine, suggesting that these substances may modulate, with minor changes, protein expression. The present study is, in part, a step toward understanding the potential smokepathogen interaction that may occur in smokers with periodontitis.