1000 resultados para conservação de germoplasma
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Pós-graduação em Doenças Tropicais - FMB
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Pós-graduação em Ciências Biológicas (Genética) - IBB
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Pós-graduação em Ciências Biológicas (Zoologia) - IBB
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Pós-graduação em Ciências Biológicas (Zoologia) - IBB
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This study aimed to evaluate the genetic diversity and relatedness of 24 accessions of Theobroma grandiflorum, originating from three units of Embrapa, aiming their use as parents in hybridization specie programs. The genetic markers used were heterologous microsatellite loci developed for cocoa. In the population studied 45 alleles were found. The effective average number of alleles per locus (2.33) was less than the average number of alleles per locus (3.21), indicating that many alleles have low frequency. The observed heterozygosity at polymorphic loci ranged from 0.33 to 1.00 with a mean of 0.54 and expected heterozygosity ranged from 0.48 to 0.76 with a mean of 0.54. The fixation index medium between loci (0.003) was not significantly different from zero. The estimate of relatedness between pairs of individuals indicates that some may be relatives, including half-brothers and clones. The results suggest that the accesses of T. grandiflorum analyzed contain a moderate level of genetic diversity and absence of inbreeding and therefore great potential for use in breeding programs.
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The aim of this study was to evaluate citric acid application with or without chitosan on the maintenance of the quality of litchi 'Bengal'. It was used litchi on mature maturation stage. After selection, fruits were immersed for one minute on the solution of citric acid and chitosan: [1]Control - without immersion; [2] citric acid 300g L-1, [3] citric acid 300 g L-1 + chitosan 0,3%, [4]citric acid 300 g L-1 + chitosan 0,6%, [5] citric acid 600 g L-1, [6] citric acid 600 g L-1 + chitosan 0,3%, [7] citric acid 600 g L-1 + chitosan 0,6%. Chitosan of low molecular weight and deacetylation degree of 75,58% (Sigma - Aldrich®) were used. After immersion, fruits were put to drain the excess of solution. Then, they were stored in cool chamber at 5ºC, previously sanitized, for 20 days. The experiment was conducted following a completely randomized design, with a factorial scheme composed by seven coating solutions and five sampling dates. Every five days were evaluated: loss of fresh mass of fruits; color; content of anthocyanins and the activity of peel enzymes polyphenoloxidase and peroxidase. It was verified that the solution of citric acid 600 g L-1, associated or not to chitosan, and the combination of chitosan 0,3% to 300 g L-1 of citric acid were the most efficient to maintain the quality of litchi 'Bengal' for 20 days at 5ºC.
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Pós-graduação em Agronomia - FEIS
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This study was carried out in Fazenda Canchim in Sao Carlos-SP, physical base of Embrapa Southeast Livestock, aiming to select, based on the agronomic characteristics of productivity of dry matter and quality of the forage, the accesses of suitable gender Paspalum for possessing potentiality of use as foraging plants. Were evaluated for 3 years, with 22 forage grasses, being 19 of Paspalum and three more of control species: Brachiaria decumbens, Andropogon gayanus cv. Baet and Panicum maximum cv. Tanzania, submitted to two intensification levels: high (fertilizer after each cut and supplemental irrigation) and low (only fertilizer of annual replacement without irrigation). The experimental design was of split blocks subdivided in the space and time with two replications. The studied variables were: dry matter production, in vitro dry matter digestibility and crude protein content. The results show that the accesses responded to the intensification level minimizing the negative effect of the stational production. Based on the studied variables, the selected accesses were: BRA-011401 (Paspalum glaucescens); BRA-011681 (P. atratum); BRA009661 (P. atratum) and BRA-019186 (P. regnellii).