Comparison of electrophoretic and dye-binding methods for measuring albumin concentrations in female turkeys

Albumin concentrations in female Bronze turkeys were compared using agarose gel electrophoresis (AGE) and the bromocresol green (BCG) dye-binding method. The correlation coefficient observed for albumin in the BCG and AGE methods was low, and statistical differences were observed at paired t test (p < 0. 0001). Compared with electrophoresis, the BCG-binding method yielded significantly lower albumin values for female turkeys during laying season. © 2011 Springer-Verlag London Limited.

Comparison of eggs, larvae, and pupae of plutella xylostella (lepidoptera: Plutellidae) as prey for podisus nigrispinus (Hemiptera: Pentatomidae)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Insect/Bt-cotton interactions: Are immunological variables and hemocyte ultrastructure in Podisus nigrispinus (Hemiptera: Pentatomidae) affected?

Recent studies have shown that ingestion by the army worm Spodoptera frugiperda of Cry1Ac toxin from Bt cotton promotes histochemical and ultrastructural changes in the digestive cells of the predatory pentatomid bug Podisus nigrispinus. Therefore, mindful of the changes in the midgut of the predator, which represents the first line of defence in this insect, our aim was to test the hypothesis that the consumption of Bt cotton-fed S. frugiperda by P. nigrispinus might lead to alterations in components of the immune system of P. nigrispinus. The Cry1Ac toxin level in the leaves of Bt cotton, nitric oxide, phenoloxidase activity, and total proteins were quantified by ELISA. Total and differential hemocyte counts were evaluated, and hemocyte ultrastructure analysis was undertaken. We found that ingestion of the prey fed daily with approximately 23 ± 0.70 ng g-1 Cry1Ac by wet weight of leaves, and expressed by the Bt cotton, induces small ultrastructural changes in the predator's granulocytes and plasmatocytes. However, these changes did not affect the total number and differential and humoral variables analyzed for the bug's hemocytes. © 2013 Copyright Taylor and Francis Group, LLC.

Control de la chinche de los pastos Collaria scenica (Hemiptera: Miridae) con nematodos entomopatógenos en invernadero

We studied the efficacy of infective juveniles (IJ) of Steinernema sp. strain JCL024 and Heterorhabditis sp. strain SL0708 on adults and nymphs of Collaria scenica under greenhouse conditions. Five doses were evaluated (2, 16, 78, 160 IJ) cm-2 leaf) for each nematode. The experiment was evaluated for 21 days every 24 hours, making mortality counts and assessment of damage on Kikuyo grass. The bugs showed 100% mortality after 15 days. Each bug produced an average of 13,000 IJ/6 days. As for the damage did not reach level 2 within 21 days prior to treatment without nematodes that damage introduced 3 with necrosis and apical leaf curl. Thus, Steinernema sp. strain JCL024 and Heterorhabditis sp. strain SL0708 exercised control bug on the grass and kept under the level of damage to the foliage.

Poly [1,1'-bis(ethynyl)-4,4'-biphenyl(bis-tributylphosphine)Pt(II)] solutions used as low dose ionizing radiation dosimeter

In this work, the effect of gamma radiation on the optical properties of polymetallayne poly[1,1'-bis(ethynyl)-4,4'-biphenyl(bis-tributylphosphine)Pt(II) ] (Pt-DEBP) in chloroform solution is studied. The samples were irradiated at room temperature with doses from 0.01 Gy to 1 Gy using a 60Co gamma ray source. A new band at 420 nm is observed in the emission spectra, in superposition to the emission maximum at 398 nm, linearly dependent on dose. We propose to use the ratio of the emission amplitude bands as the dosimetric parameter. This method proved to be robust, accurate, and can be used as a dosimeter in medical applications. © 2013 AIP Publishing LLC.

Synthesis and characterization of melanin in DMSO

Recently soluble melanin derivatives have been obtained by a synthetic procedure carried out in DMSO (D-melanin). In this work a comparative study of the structural characteristics of synthetic melanin derivatives obtained by oxidation of L-DOPA in H2O and DMSO are presented. To this end, Fourier-transform infrared spectroscopy as well as proton and carbon nuclear magnetic resonance techniques has been employed. In addition, aging effects have been investigated for D-melanin. The results suggest that sulfonate groups (-SO2CH3) from the oxidation of DMSO, are incorporated into melanin, which confers protection to the phenolic hydroxyl group present in its structure. The solubility of D-melanin in DMSO is attributed to the presence of these groups. When D-melanin is left in air for long time periods, the sulfonate groups leave the structure, and an insoluble compound is obtained. NaOH and water have been used, in order to accelerate the release of the sulfonate groups attached to D-melanin, thereby corroborating the proposed structure and the synthesis mechanism. © 2013.

Interações de aranhas (Araneae) e artrópodes-praga (Acari e Hemiptera) em cultivos comerciais de seringueira (Hevea brasiliensis) no Noroeste do Estado de São Paulo

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Aspectos biológicos e taxa de consumo de Orius insidiosus (Say, 1832) (Hemiptera: Anthocoridae) predando Plutella xylostella (L., 1758) (Lepidoptera: Plutellidae)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Influência dos estressores bióticos Diatraea saccharalis (Fabr.) (Lepidoptera: Crambidae) e Mahanarva fimbriolata (Stål) (Hemiptera: Cercopidae) na produtividade e qualidade tecnológica da cana-de-açúcar

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Características biológicas de Orius insidiosus alimentado com Aphis gossypii em algodão transgênico e convencional

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Resistência de genótipos de soja Glycine max (L.) a Nezara viridula (L. ,1758) (Hemiptera: Pentatomidae)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Influência de sistemas de semeadura na população de pragas e nas características morfofisiológicas em cultivares de soja

Pós-graduação em Agronomia (Proteção de Plantas) - FCA

Controle do percevejo castanho (Scaptocoris castanea Perty, 1830 (Hemiptera: Cydnidae)) na cultura da soja (Glycine max (L.) Merrill)

Pós-graduação em Agronomia (Proteção de Plantas) - FCA

Bionomia e comportamento de atopozelus opsimus Elkins (Hemiptera reduviidae) mantidos em glycaspis brimblecombei Moore (Hemiptera : Psyllidae)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Efeito de regulador vegetal sobre a brotação, florescimento e frutificação de cultivares de goiabeira

Pós-graduação em Agronomia - FEIS