Overexpression and Purification of the Oat (Avena Fatua) Protein AFN1 and Construction of a pMAL/AFN1 Expression Plasmid

Abscisic acid (ABA) is an important phytohormone with regulatory roles in many physiological processes. ABA expression is induced by environmental stresses such as drought and it is known to be an inhibitor of seed germination. A wild oat (Avena fatua) called AFN1 has been hypothesized to initiate the early stages of germination as its mRNA accumulates in nondormant seed embryos during imbibition. The polypeptide sequence of AFN1 suggests that it is an ABA glucosyl transferase. Glucosylation by AFN1 and thereby inactivation of ABA could lead to seed germination. In order to understand the role of AFN1 in germination, an ample quantity of AFN1 polypeptide is needed to test for enzymatic ABA glucosylase activity. My work has been to overexpress recombinant AFN1containing a (His)6 tag using a pRSETC E.coli expression system followed by Purification of the AFN1 protein by means of a nickel-affinity column that bind to the (His)6 tag. Due to the insufficient yield of AFN1 fusion protein obtained with this procedure, another method using a pMAL-c2x vector is now being employed. The pMAL expression system provides a method for expressing and purifying protein by tagging proteins with maltose-binding protein (MBP). It is anticipated that MBP tag will be advantageous as it can make the fusion protein more soluble and thereby yield a larger quantity of protein. Currently, work is underway on the construction of pMAL/AFN1 plasmid.

August 1999: Financial Black Holes and Other Challenges

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Collaboration Is the New Black

Orange may be the new black, but as I have seen only five minutes of that show, I can’t really use it here. Besides, based on the five minutes I saw, I would assume it is a series written by males. Not since the Victoria’s Secret catalog have I seen so many women wearing fewer clothes, or engaging in so many unmentionable acts. I’ll stop there because my Victorianism is showing, I’m sure.

Aplicações do modelo black-scholes ao mercado de opções de compra, utilizando-se metodologias diferenciais para o cálculo de volatilidade

Este trabalho cuida de avaliar a eficiência do mercado de opções de ações da bolsa de valores de são Paulo (BOVESPA). A avaliação é feita através do modelo Black-Scholes, e traz como principal novidade diversas estimativas de volatilidade. Portanto torna-se um teste conjunto da eficiência do mercado, do modelo Black-Scholes e das diversas estimativas de volatilidade. O objetivo principal ~ determinar a volatilidade que gera o melhor retorno , isto é , aponta a maior ineficiência do mercado. Foram utilizadas opções de Paranapanema-pp e Petrobr's-pp no per(odo de novembro de 1987 a outubro de 1988. Dois testes de eficiência foram realizados para cada volatilidade estimada . Em ambos observou-se que o mercado é ineficiente, e no segundo obtivemos evidência de que uma das estimativas de volatilidade gera um retorno maio