Moedas sociais digitais: estudo de caso de duas experiências em bancos comunitários

Este trabalho parte de duas experiências de implantação de moedas sociais digitais pelo poder público para responder se a introdução de uma tecnologia de pagamentos digital, em conjunto com moedas sociais, pode modificar conceitos básicos de comunidade e sociedade ou se é a tecnologia que precisa se adaptar a estes conceitos. Com os casos representando polos opostos na teoria de Tönnies em termos de comunidade/sociedade, têm-se como hipótese que a introdução da tecnologia em moedas sociais precisa estar em linha com conceitos básicos de comunidade, solidariedade, pertencimento, entre outros, de acordo com o frame de cada experiência. Como resultado, há poucas evidências que possam ser tidas neste momento como definitivas. Resta especular que o uso da tecnologia de pagamentos digitais como forma de ganho de escala do modelo de economia solidária em sociedades do tipo gemeinschaft encontra falta de elementos facilitadores que incentivem sua implementação e uso, sendo que, novamente, indaga-se que as tecnologias disponíveis de moedas sociais digitais parecem ser pouco congruentes com o próprio conceito de comunidade que faz parte do cerne da economia solidária e de sociedades locais do tipo gemeinschaft, podendo haver maior proximidade com sociedades do tipo gesellschaft. Em adição a isto, especula-se que (1) mesmo havendo êxito na implantação de moedas sociais digitais em comunidades do tipo gemeinschaft, que isto somente se confirmará mediante a criação de um modelo inovador de tecnologia de pagamentos que leve em conta o capital social dessas localidades; (2) as tecnologias hoje existentes para confecção de uma moeda social digital tendem a um ganho de escala que pode mostrar alguma incompatibilidade com pequenas comunidades onde o senso solidário é maior que o individual; (3) forçar a adoção de moedas sociais digitais em pequenas comunidades do tipo gemeinschaft pode eventualmente levar a um maior grau de individualização e à contratualização das relações interpessoais, dado que hoje muitas das relações nestas comunidades contêm alto grau de pessoalidade e informalidade; (4) havendo uma implementação de moedas sociais digitais de cima para baixo, este modelo tende a ser mais propício para experiências liberais focadas nos indivíduos do que para experiências comunitárias focadas no desenvolvimento do modelo pela própria comunidade. Como contribuição teórica, o estudo tenta mostrar o potencial do uso da teoria de Tönnies com o conceito de frames de Goffman.

Biosystematics of the Genus Andryala L. (Asteraceae)

Andryala (Asteraceae: Cichorieae) is a little-known Mediterranean-Macaronesian genus whose taxonomy is much in need of revision. The aim of the present biosystematic study was to elucidate species relationships within this genus based on morphological and molecular data. In this study several taxa are recognised: 17 species, 14 subspecies, and 3 hybrids. Among these, 5 species are Macaronesian endemics (A. glandulosa, A. sparsiflora, A. crithmifolia Aiton, A. pinnatifida, and A. perezii), 4 species are Northwest African endemics (A. mogadorensis, A. maroccana, A. chevallieri, and A. nigricans) and one species is endemic to Romania (A. laevitomentosa). Historical background regarding taxonomic delimitation in the genus is addressed from Linnaean to present day concepts, as well as the origin of the name Andryala. The origin of Asteraceae and the systematic position of Andryala is shortly summarised. The morphological study was based on a bibliographic review and the revision of 1066 specimens of 13 herbaria as well as additional material collected during fieldwork. The variability of the morphological characters of the genus, including both vegetative taxonomic characters (root, stem, leaf and indumentum characters) and reproductive ones (inflorescence, floret, fruit and pappus characters), is assessed. Numerical analysis of the morphological data was performed using different similarity or dissimilarity measures and coefficients, as well as ordination and clustering methods. Results support the segregation of the recognised taxa and the congruence of the several analyses in the separation of the recognised taxa (using quantitative, binary or multi-state characters). The proposed taxonomy for Andryala includes a new infra-generic classification, new taxa and new combinations and ranks, typifications and diagnostic keys (one for the species and several for subspecies). For each taxon a list of synonyms, typification comments and a detailed description are provided, just as comments on taxonomy and nomenclature, and a brief discussion on karyology. Additionally, information on ecology and conservation status as well as on distribution and a list of studied material are also presented. Phylogenetic analyses based on different nuclear and chloroplast DNA markers, using Bayesian and maximum parsimony methods of inference, were performed. Results support three main lineages: separate ones for the relict species A. agardhii and A. laevitomentosa and a third including the majority of the Andryala species that underwent a relatively rapid and recent speciation. They also suggest a single colonization event of Madeira and the Canary Islands from the Mediterranean region, followed by insular speciation. Biogeography and speciation within the genus are briefly discussed, including a proposal for the centre of origin of the genus and possible dispersal routes.

Caracterização funcional de dois cDNAs homólogos à ciclofilina e à proteína reprimida por auxina (ARP) identificados em bibliotecas subtrativas a para floração em tomateiro

Flowering is a fundamental process in the life cycle for plant. This process is marked by vegetative to reproductive apical meristem conversion, due to interactions between several factors, both internal and external to plant. Therefore, eight subtractive libraries were constructed using apical meristem induced or not induced for two contrasting species: Solanum lycopersicum cv. Micro-Tom and Solanum pimpinellifolium. Several cDNAs were identified and among these, were selected two cDNAs: one homologous cDNA to cyclophilin (LeCYP1) and the other to Auxin repressed protein (ARP). It has observed that LeCYP1 and ARP genes are important in the developmental process to plants. In silico analysis, were used several databases with the exclusion criterion E-value <1.0x10-15. As a result, conservation was observed for proteins analyzed by means of multiple alignments and the presence of functional domains. Then, overexpression cassettes were constructed for the ARP cDNA in sense and antisense orientations. For this step, it was used the CaMV35S promoter. The cDNA orientation (sense or antisense) in relation to the promoter was determined by restriction enzymes and sequencing. Then, this cassette was transferred to binary vector pZP211 and these cassettes were transferred into Agrobacterium tumefaciens LBA4404. S. lycopersicum cv. Micro-Tom (MT) and MT-Rg1 plants were transformed. In addition, seedlings were subjected to hormone treatments using a synthetic auxin (- naphthalene acetic acid) and cyclosporin A (cyclophilin inhibitor) treatments and it was found that the hormone treatment there were changes in development of lateral roots pattern, probably related to decreases in auxin signaling caused by reduction of LeCYP1 in MT-dgt plants while cyclosporin A treatments, there was a slight delay in flowering in cv. MT plants. Furthermore, assay with real-time PCR (RT-qPCR) were done for expression level analysis from LeCYP1 and ARP in order to functionally characterize these sequences in tomato plants.

Estudo da sinterização de eletrólito sólido de céria dopada com gadolínia

Fuel cells are electrochemical devices that convert chemical energy in electrical energy by a reaction directly. The solid oxide fuel cell (SOFC) works in temperature between 900ºC up to 1000ºC, Nowadays the most material for ceramic electrolytes is yttria stabilized zirconium. However, the high operation temperature can produce problems as instability and incompatibility of materials, thermal degradation and high cost of the surround materials. These problems can be reduced with the development of intermediate temperature solid oxide fuel cell (IT-SOFC) that works at temperature range of 600ºC to 800ºC. Ceria doped gadolinium is one of the most promising materials for electrolytes IT-SOFC due high ionic conductivity and good compatibility with electrodes. The inhibition of grain growth has been investigated during the sintering to improve properties of electrolytes. Two-step sintering (TSS) is an interesting technical to inhibit this grain growth and consist at submit the sample at two stages of temperature. The first one stage aims to achieve the critical density in the initiating the sintering process, then the sample is submitted at the second stage where the temperature sufficient to continue the sintering without accelerate grain growth until to reach total densification. The goal of this work is to produce electrolytes of ceria doped gadolinium by two-step sintering. In this context were produced samples from micrometric and nanometric powders by two routes of two-step sintering. The samples were obtained with elevate relative density, higher than 90% using low energy that some works at the same area. The average grain size are at the range 0,37 μm up to 0,51 μm. The overall ionic conductivity is 1,8x10-2 S.cm and the activation energy is 0,76 eV. Results shown that is possible to obtain ceria-doped gadolinium samples by two-step sintering technique using modified routes with characteristics and properties necessary to apply as electrolytes of solid oxide fuel cell

Análise de cDNAs identificados em bibliotecas substrativas de cDNA para floração de variedades de cana-de-açúcar cultivadas no Rio Grande do Norte(RN): fator de transição NAC, Calmodulina e Fosfatidiltransferase

The flowering is a physiological process that it is vital for plants. This physiological process has been well studied in the plant model Arabidopsis, but in sugarcane this process is not well known. The transition of the shoot apical meristem from vegetative to flowering is a critical factor for plant development. At Brazil northeastern region, the transition to flowering in sugarcane has an important effect as it may reduce up to 60% its production. This is a consequence of the sugar translocation from stalks to the shoot apical meristem which is necessary during the flowering process. Therefore, the aim of this work was to explore and analyze cDNAs previously identified using subtractive cDNA libraries. The results showed that these cDNAs showed differential expression profile in varieties of sugarcane (early x late flowering). The in silico analysis suggested that these cDNAs had homology to calmodulin, NAC transcription factor and phosphatidylinositol, a SEC14, which were described in the literature as having a role in the process of floral development. To better understand the role of the cDNA homologous to calmodulin, tobacco plants were transformed with overexpression cassettes in sense and antissense orientation. Plants overexpressing the cassette in sense orientation did not flowered, while plants overexpressing the cassette in the antissense orientation produced flowers. The data obtained in this study suggested the possible role from CAM sequence, SEC14 and NAC in the induction/floral development pathway in sugarcane, this is the first study in order to analyze these genes in the sugarcane flowering process.

Enraizamento de estacas de Camellia sinensis L. em função da época de coleta de ramos, genótipos e ácido indolbutírico

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

The bacterial chromosome segregation protein Spo0J spreads along DNA from parS nucleation sites

Regulation of chromosome inheritance is essential to ensure proper transmission of genetic information. To accomplish accurate genome segregation, cells organize their chromosomes and actively separate them prior to cytokinesis. In Bacillus subtilis the Spo0J protein is required for accurate chromosome segregation and it regulates the developmental switch from vegetative growth to sporulation. Spo0J is a DNA-binding protein that recognizes at least eight identified parS sites located near the origin of replication. As judged by fluorescence microscopy, Spo0J forms discrete foci associated with the oriC region of the chromosome throughout the cell cycle. In an attempt to determine the mechanisms utilized by Spo0J to facilitate productive chromosome segregation, we have investigated the DNA binding activity of Spo0J. In vivo we find Spo0J associates with several kilobases of DNA flanking its specific binding sites (parS) through a parS-dependent nucleation event that promotes lateral spreading of Spo0J along the chromosome. Using purified components we find that Spo0J has the ability to coat non-specific DNA substrates. These 'Spo0J domains' provide large structures near oriC that could potentially demark, organize or localize the origin region of the chromosome.

Host specificity of a Brazilian isolate of Alternaria cassiae (Cenargen CG593) under greenhouse conditions

Several Alternaria cassiae isolates were recovered from diseased sicklepod plants (Senna obtusifolia) in the southern regions of Brazil. A representative isolate (Cenargen CG593) was tested for its host range under greenhouse conditions. The fungus promoted symptoms in sicklepod, cassava (Manihot dulce), tomato (Lycopersicon esculentum) and eggplant (Solanum melongena) when tested at a spore concentration of 10(6) spores ml(-1). When the plants were inoculated with a suspension of 10(5) spores ml(-1) and held at a dew period of 12 h (cassava) or 18 h (tomato and eggplant), the plants showed symptoms of the disease, but they recovered and continued their normal vegetative growth. These results show that the fungus A. cassiae is safe to use for the control of S. obtusifolia under Brazilian conditions, because it did not cause excessive damage in the three plants tested.

Identificação e caracterização de genes vip e cry coleóptero-específicos em isolados de Bacillus thuringiensis

O objetivo deste trabalho foi identificar e caracterizar os genes cry3, vip1, vip2 e vip1/vip2 em uma coleção de 1.078 isolados de Bacillus thuringiensis potencialmente tóxicos para larvas de coleópteros. Foram utilizados pares de oligonucleotídeos iniciadores gerais obtidos a partir de regiões conservadas dos genes e do alinhamento de sequências consenso. Posteriormente, os isolados positivos foram caracterizados por meio da técnica de PCR‑RFLP, tendo-se utilizado enzimas de restrição específicas, para identificar novas subclasses de genes nos isolados. Cento e cinquenta e um isolados foram positivos para os genes avaliados, com maior frequência para o gene vip1/vip2 (139 isolados). Pela técnica de PCR‑RFLP, foram observados 14 perfis polimórficos, o que indica a presença de diferentes alelos e, consequentemente, de distintas subclasses desses genes.

Efeitos da solarização do solo através de plástico transparente sobre o desenvolvimento da tiririca (Cyperus rotundus)

Um ensaio foi conduzido em condições de campo, durante o verão, com o objetivo de avaliar o potencial da técnica de solarização do solo no controle da tiririca (Cyperus rotundus L.). O delineamento experimental utilizado foi o de blocos casualizados com três repetições, e os tratamentos num esquema fatorial 2 x 2 x 3, sendo duas situações de cobertura (com e sem plástico transparente de 300 mm de espessura), dois estádios de desenvolvimento da planta daninha (vegetativo e florescimento) e três períodos de cobertura com plástico (15, 30 e 60 dias). A temperatura do solo sob solarização teve um aumento médio de 4,3oC em relação à testemunha, atingindo valores superiores a 50oC em determinados horários. Observou-se, também, um acúmulo da ordem de 400 % nos teores de CO2 na atmosfera do solo solarizado. Nessas condições houve inibição da brotação dos tubérculos e diminuição no peso de matéria seca de todas as partes estudadas da planta. Houve redução da taxa de multiplicação dos tubérculos, reduzindo-a de 1:11 para 1:4, quando coberta no estádio vegetativo e para 1:9 quando a cobertura se realizou no estádio de florescimento. Houve, ainda diminuição na ordem de 20% na viabilidade dos tubérculos remanescentes.

Estudo de pré-formulação para dose fixa combinada dos tuberculostáticos rifampicina, isoniazida, pirazinamida e etambutol (4 em 1)

According to the global framework regarding new cases of tuberculosis, Brazil appears at the 18th place. Thus, the Ministry of Health has defined this disease as a priority in the governmental policies. As a consequence, studies concerning treatment and prevention have increased. Fixed-dose combination formulations (FDC) are recognized as beneficial and are recommended by WHO, but they present instability and loss on rifampicin bioavailability. The main purpose of this work was to carry out a pre-formulation study with the schedule 1 tuberculosis treatment drugs: rifampicin, isoniazid, pyrazinamide and ethambutol and pharmaceutical excipients (lactose, cellulose, magnesium stearate and talc), in order to develop an FDC product (150 mg of rifampicin + 75 mg of isoniazid + 400 mg of pyrazinamide + 250 mg of ethambutol). The studies consisted of the determination of particle size and distribution (Ferret s diameter) and shape through optical microscopy, as well as rheological and technological properties (bulk and tapped densities, Hausner Factor, Carr s Index, repose angle and flux rate) and interactions among drugs and drug excipient through thermal analysis (DSC, DTA, TG and your derivate). The results showed that, except isoniazid, the other drugs presented poor rheological properties, determined by the physical characteristics of the particles: small size and rod like particles shape for rifampicin; rectangular shape for pyrazinamide and ethambutol, beyond its low density. The 4 drug mixture also not presented flowability, particularly that one containing drug quantity indicated for the formulation of FDC products. In this mixture, isoniazid, that has the best flowability, was added in a lower concentration. The addition of microcrystalline cellulose, magnesium stearate and talc to the drug mixtures improved flowability properties. In DSC analysis probable interactions among drugs were found, supporting the hypothesis of ethambutol and pyrazinamide catalysis of the rifampicin-isoniazid reaction resulting in 3- formylrifamycin isonicotinyl hydrazone (HYD) as a degradation product. In the mixtures containing lactose Supertab® DSC curves evidenced incompatibility among drugs and excipient. In the DSC curves of mixtures containing cellulose MC101®, magnesium stearate and talc, no alterations were observed comparing to the drug profiles. The TG/DTG of the binary and ternary mixtures curves showed different thermogravimetrics profiles relating that observed to the drug isolated, with the thermal decomposition early supporting the evidences of incompatibilities showed in the DSC and DTA curves

Efeito de níveis crescentes de calagem no crescimento e estado nutricional de fedegoso

A distribuição e desenvolvimento de plantas, a exemplo daquelas que infestam as áreas agrícolas, é influenciada por diversos fatores, a como aqueles ligados às caraterísticas do solo, naturais ou impostas pelo homem. Assim, o presente trabalho de pesquisa foi desenvolvido em condições de casa-de-vegetação com o objetivo de estudar o efeito de níveis crescentes de calagem no crescimento e estado nutricional de Senna obtusifolia, planta daninha comum em ecossistemas agrícolas. O delineamento experimental adotado foi o inteiramente casualizado, com seis tratamentos e quatro repetições. Os tratamentos constituíram-se em quantidades de calcário dolomítico calcinado com 38% de CaO e 20% de MgO, com um poder de neutralização total (PRNT) de 104%: 0, 2, 4, 6, 8 e 10 t/ha. As parcelas experimentais constituíram-se de recipientes de plástico com capacidade de 5 litros, preenchidos com terra coletada na camada arável de um latossolo vermelho escuro distrófico, A moderado, textura média. Após um período de crescimento de 56 dias as plantas foram coletadas e separadas em seus componentes vegetativos, sendo colocados a secar em estufa de circulação forçada de ar (70ºC por 72 horas) para determinação do peso seco. O material foi pesado e moído e analisado a nível de composição de nutrientes. Os resultados mostraram efeitos negativos dos níveis crescentes de calcário, sobre o crescimento das plantas de S. obtusifolia, evidenciando-se decréscimos nos valores da matéria seca acumulada a partir da dose de 4 t/ha de calcário (pH 5,8), com efeitos mais marcantes nas raízes. A aplicação de doses crescentes de calcário promoveu um aumento progressivo nos teores de Ca e Mg no solo, o que talvez possa ter estimulado a competição entre cátions, reduzindo a absorção do K. Os elementos N e P foram os mais absorvidos pela planta.

Half-sib progenies evaluation in velvet grass

O capim-lanudo (Holcus lanatus L.) possui grande potencial de utilização como pastagem de inverno nas regiões subtropicais, devido a seu bom estabelecimento, persistência, produção, resistência ao frio, palatabilidade e capacidade de afilhamento. O objetivo deste trabalho foi estimar a variabilidade genética e a herdabilidade de caracteres agronômicos e identificar progênies superiores. Foram avaliadas 60 progênies de meios-irmãos, para altura (NH) e diâmetro da planta na fase vegetativa, duração do ciclo vegetativo (ciclo), altura final (FH) e número de afilhos. Houve diferença entre progênies para todos os caracteres. As estimativas de herdabilidade foram de 38%, 32%, 92%, 57% e 64% para NH, diâmetro, ciclo, FH e afilhos, respectivamente. O maior ganho genético estimado foi de 30,77 % para o número de afilhos. Existe variabilidade para todos os caracteres e são esperados ganhos na seleção entre progênies.

Distribuição do sistema radicular de porta-enxertos de umezeiro enxertados com o pessegueiro 'Aurora-1'

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)