Studies on the electrophoretic patterns of fish muscle myogens

Electrophoretic patterns of the muscle myogens of prawns and some common Indian food fishes of marine and fresh water origin were obtained on polyacrylamide gel. It was observed that the characteristic band pattern which was species specific and was not altered by storage of fish in ice could be employed as a means of identification of fish species.

Freeze drying of fishery products: Part IV - Biochemical changes occurring in prawn muscle during freezing, freeze drying and prolonged storage of the freeze dried product

The changes occurring in water and salt extractable protein and non-protein fractions in prawn muscle of different species during freezing, freeze drying and subsequent prolonged storage have been studied. There is no denaturation of water extractable proteins, whereas salt extractable proteins were rendered insoluble to the extent of 21% due to freeze drying. The freeze dried products remained in good edible condition for 32 months of storage up to which storage characteristics were followed.

Fish muscle structural proteins

Based on the differences in their physico-chemical properties proteins are broadly categorised as sarcoplasmic or fibrillar. The properties are important in fish technology. The authors review some of these physico-chemical properties of the proteins which have distinguished themselves as 'structural' or 'textural' proteins. As denaturation in these proteins caused by a variety of factors manifests itself in terms in the quality attributes of the product, relevant mechanisms have been particularly highlighted.

Some physical properties of the muscle lipid of two freshwater teleosts, Ophicephalus striatus (Bloch.) and Clarias batrachus (Linn.)

Various physical properties (viscosity, fluidity, surface tension and specific gravity) have been determined for muscle lipids of Ophicephalus striatus and Clarias batrachus. Results are presented and the methods used in determination noted. The physical parameters studied are found to be species-specific.

Some changes in the physical qualities of muscle lipid of the freshwater murrel, Ophicephalus striatus (bloch.) during storage

The present communication reports the changes in the specific gravity, coefficient of viscosity, fluidity and surface tension of the muscle lipid of O. striatus, a common freshwater murrel, when stored at room temp (32 ± 2°C) The specific gravity of muscle lipid was found to rise from 0.894 to 0.912 during the first 25 days of storage but registered the highest (0.925) when stored for 50 days. Surface tension seemed to rise with the duration of storage. This was, presumably, due to an increase in the forces with which the molecules in the surface of the lipid tended to compress the molecules below to the smallest possible volume. During the period of storage marked changes seemed to occur in the direction of an increase in the value of the coefficient of viscosity and a reciprocal decline in the fluidity. Evidently, the observed increase in the viscosity seemed to be the result of increased internal friction between different molecular layers of the lipid, whereas a decline in the fluidity was perhaps the consequence of its inverse correlation with the coefficient of viscosity.

Purification and characterisation of phosphorylase from muscle of Tilapia (Tilapia mosambica)

Phosphorylase from muscle of tilapia (Tilapia mosambica) was extracted by water and purified by ammonium sulphate precipitation, centrifugation and repeated recrystallisation. Electro-phorogram of the enzyme preparation showed a single band near origin. The enzyme showed optimum pH and temperature at 6.1 and 37°C respectively. Glucose and glucose-6-phosphate were found to be competitive inhibitors of the enzyme. Maltose and starch acted as primers for the phosphorylase reaction like glycogen.

Further studies on changes in protein fractions of fish muscle during storage in ice

The changes in the major protein nitrogen fractions of two commercially important fishes of Indian waters, viz., mackerel (Rastrelliger kanagurta) and lactarius (Lactarius lactarius), during storage in ice are reported. The significance of the findings is discussed in comparison with the results of a similar study on two species of marine prawns and oil sardine, reported earlier.

Influence of muscle fats on the quality of intermediate moisture fish

Chrysicthys nigrodigitatus with 12.95% fat having an iodine value of 74.8 and a saponification number of 198.48 and Citharinus citherus containing only 3.25% fat with iodine value of 67.8 and a saponification number of 145.86 were studied as examples of fatty and lean fishes respectively. The intermediate moisture (IM) products of both fish types compared with normal cooked samples, were evaluated as of acceptable colour, odour, texure and juiciness but of inferior taste due to the glycerol impact. However, during storage at 30°C the IM products became increasingly less acceptable with the deterioration being greater in the fatty fish than in the lean fish, although the fatty IM fish was superior to the IM lean fish with regard to water retention and juiciness. Overall quality differences were most apparent in colour and odour with the fatty IM fish being worse. The fatty fish had also greater evolution of TEA-reactive carbonyl breakdown products of lipid oxidation which were subsequently used up in non-enzymic browning producing the correspondingly darker fish colour and greater off odour.

Changes in the muscle of three Indian major carps during frozen storage

Chunks of Labeo rohita, Cirrhinus mrigala and Catla catla wrapped in polythene film were stored at -8 to -10°C in the freezer cabinet of the refrigerator. It was found that L. rohita and C. mrigala were acceptable up to 33 days and C. catch up to 35 days. Total volatile base nitrogen, free fatty acids and degree of sponginess of the samples showed increasing trend during frozen storage.

Temporal behaviour of marine landings along coastal Karnataka 1. Relative distribution and secular trends

Landing data of 21 fisheries from Karnataka (quarterly landings for the period 1956 to 1978 and annual landings for the period 1979 to 1981) were analysed to study the te111poral behaviour of the landings. The relative distribution and secular trends in each of the fisheries are reported. On an average, mackerel and oil sardine together account for about 70% of the annual landings. The fluctuation in the landings in all the fisheries was very high with the coefficient of variation attaining a minimum value of 61.53%. The inverse relationship between the landings of oil sardine and mackerel was found to be statistically significant. It is suggested that fishing effort be further increased in fisheries that have demonstrated a rising trend (about 73% of the total annual landings) as also in the case of the fisheries whose trends appear stagnant (about 21% of the annual landings). However, there seems to be some evidence of a need for regulating fishing in Hemirhamphus and Belone, Caranx and penaeid prawns. Regulations need to be made authentic with the help of sufficient information on not merely the fishery but on the whole population.

Temporal behaviour of marine landing in Karnataka 2. Seasonal patterns

Seasonal patterns of 21 fisheries in Karnataka (after isolation from time-series components) are presented. Depending on the pattern of seasonal fluctuation in landings, 19 fisheries have been grouped into five patterns, A, B, C, D and E. Ribbon fishes and 'other clupeids' did not exhibit any significant seasonal pattern. Pattern A with highest landings in the 4th quarter (October to December), followed by the 1, 2 and 3 quarters, is the most common in 10 species/groups (comprising 78% of the total landings). Harmonic analysis has been carried out using the seasonal indices.

Temporal behaviour of marine landings along coastal Karnataka 3. Cyclical patterns

Marine landing data for Karnataka during 1956-1978 were subjected to time series analysis and cyclical periodicities isolated in the case of seven fisheries namely, ribbon fish (five year cycle); Caranx spp., Leiognathus spp, and mackerel (six year cycle each); the combined landings of Hemirhamphus spp. and Belone spp.,'Lesser Sardines' and 'Other Clupeids' (seven year cycle each). 'Total' demonstrated an eight-year cyclical periodicity.