Genomic Strategy Identifies a Missense Mutation in WD-Repeat Domain 65 (WDR65) in an Individual With Van der Woude Syndrome

Genetic variation in the transcription factor interferon regulatory factor 6 (IRF6) causes and contributes risk for oral clefting disorders. We hypothesized that genes regulated by IRF6 are also involved in oral clefting disorders. We used five criteria to identify potential IRF6 target genes; differential gene expression in skin taken from wild-type and Irf6-deficient murine embryos, localization to the Van der Woude syndrome 2 (VWS2) locus at 1p36-1p32, overlapping expression with Irf6, presence of a conserved predicted-binding site in the promoter region, and a mutant murine phenotype that was similar to the Irf6 mutant mouse. Previously, we observed altered expression for 573 genes; 13 were located in the murine region syntenic to the VWS2 locus. Two of these genes, Wdr65 and Stratifin, met 4 of 5 criteria. Wdr65 was a novel gene that encoded a predicted protein of 1,250 amino acids with two WD domains. As potential targets for Irf6 regulation, we hypothesized that disease-causing mutations will be found in WDR65 and Stratifin in individuals with VWS or VWS-like syndromes. We identified a potentially etiologic missense mutation in WDR65 in a person with VWS who does not have an exonic mutation in IRF6. The expression and mutation data were consistent with the hypothesis that WDR65 was a novel gene involved in oral clefting. (C) 2011 Wiley-Liss, Inc.

Amphibians and reptiles from a highly diverse area of the Caatinga domain: composition and conservation implications

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Mutational analysis of the GAP-related domain of the neurofibromatosis type 1 gene in Brazilian NF1 patients

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

DODAB and DODAC bilayer-like aggregates in the micromolar surfactant concentration domain

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Scanning electron microscope evaluation of chlorhexidine gel and liquid associated with sodium hypochlorite cleaning on the root canal walls

Objective. The aim of this study was to evaluate, by scanning electronic microscopy (SEM), the cleaning of the root canal walls after instrumentation and irrigation with 2.5% sodium hypochlorite (NaOCl) associated with 2% chlorhexidine (CHX) gel or liquid, combined or not with 17% ethylenediamine tetra-acetic acid (EDTA).Study design. Sixty single-root human teeth were subjected to standardized root canal instrumentation with different irrigants (n = 10): G1) NaOCl + CHX liquid; G2) NaOCl + CHX liquid + EDTA + saline solution; G3) NaOCl + CHX gel; G4) NaOCl + CHX gel + EDTA + saline solution; G5) saline solution; G6) saline solution + EDTA. After instrumentation, the teeth were prepared for SEM analysis (x500 and x2,000) to evaluate the cleaning of the cervical, middle, and apical thirds. The area analyzed was quantified according to the percentage of open and closed tubules, and data were statistically analyzed by analysis of variance and Tukey tests (P = .05).Results. The number of open tubules was highest in G4 in all root thirds, showing statistically significant difference from G1, G2, and G5 (P < .05). G1 presented higher quantity of closed tubules significant than G2.Conclusion. Irrigation with NaOCl and CHX gel followed by EDTA and saline solution produced greater cleaning of the root canal walls. (Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2010;110:e82-e87)

Ten-dimensional supergravity constraints from the pure spinor formalism for the superstring

It has recently been shown that the ten-dimensional superstring can be quantized using the BRST operator Q = philambda(alpha)d(alpha), where lambda(alpha) is a pure spinor satisfying; lambdagamma(m)lambda = 0 and dalpha is the fermionic supersymmetric derivative. In this paper, the pure spinor version of superstring theory is formulated in a curved supergravity background and it is shown that nilpotency and holomorphicity of the pure spinor BRST operator imply the on-shell superspace constraints of the supergravity background. This is shown to lowest order in alpha' for the heterotic and Type II superstrings, thus providing a compact pure spinor version of the ten-dimensional superspace constraints for N = 1 Type IIA and Type IIB supergravities. Since quantization is straightforward using the pure spinor version of the superstring, it is expected that these methods can also be used to compute higher-order alpha' corrections to the ten-dimensional superspace constraints. (C) 2002 Elsevier B.V. B.V. All rights reserved.

The two-loop massless (lambda/4!)phi(4) model in nontranslational invariant domain

We study the (lambda/4!)phi(4) massless scalar field theory in a four-dimensional Euclidean space, where all but one of the coordinates are unbounded. We are considering Dirichlet boundary conditions in two hyperplanes, breaking the translation invariance of the system. We show how to implement the perturbative renormalization up to two-loop level of the theory. First, analyzing the full two and four-point functions at the one-loop level, we show that the bulk counterterms are sufficient to render the theory finite. Meanwhile, at the two-loop level, we must also introduce surface counterterms in the bare Lagrangian in order to make finite the full two and also four-point Schwinger functions. (c) 2006 American Institute of Physics.

Linear relations between N >= 4 supergravity and subleading-color SYM amplitudes

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Higgsing M2 to D2 with gravity: N=6 chiral supergravity from topologically gauged ABJM theory

We present the higgsing of three-dimensional N = 6 superconformal ABJM type theories coupled to conformal supergravity, so called topologically gauged ABJM theory, thus providing a gravitational extension of previous work on the relation between N M2 and N D2-branes. The resulting N = 6 supergravity theory appears at a chiral point similar to that of three-dimensional chiral gravity introduced recently by Li, Song and Strominger, but with the opposite sign for the Ricci scalar term in the lagrangian. We identify the supersymmetry in the broken phase as a particular linear combination of the supersymmetry and special conformal supersymmetry in the original topologically gauged ABJM theory. We also discuss the higgsing procedure in detail paying special attention to the role played by the U(1) factors in the original ABJM model and the U(1) introduced in the topological gauging.

On KLT and SYM-supergravity relations from 5-point 1-loop amplitudes

We derive a new non-singular tree-level KLT relation for the n = 5-point amplitudes, with manifest 2(n-2)! symmetry, using information from one-loop amplitudes and IR divergences, and speculate how one might extend it to higher n-point functions. We show that the subleading-color N = 4 SYM 5-point amplitude has leading IR divergence of 1/epsilon, which is essential for the applications of this paper. We also propose a relation between the subleading-color N = 4 SYM and N = 8 supergravity 1-loop 5-point amplitudes, valid for the IR divergences and possibly for the whole amplitudes, using techniques similar to those used in our derivation of the new KLT relation.

Type I supergravity effective action from pure spinor formalism

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

ELKO SPINOR FIELDS: LAGRANGIANS FOR GRAVITY DERIVED FROM SUPERGRAVITY

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Relics of eclogite facies assemblages in the Ceara Central Domain, NW Borborema Province, NE Brazil: Implications for the assembly of West Gondwana

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)