Ms. Barth. 77 - De laudibus beatae Mariae virginis libri XII

Vorbesitzer: Dominikanerkloster Frankfurt am Main; Bartholomaeusstift Frankfurt am Main

Interactions of Polyvinylpyrrolidone with Chlorin e6-Based Photosensitizers Studied by NMR and Electronic Absorption Spectroscopy

Polyvinylpyrrolidone (PVP) can act as potential drug delivery vehicle for porphyrin-based photosensitizers in photodynamic therapy (PDT) to enhance their stability and prevent porphyrin self-association. In the present study the interactions of PVP (MW 10 kDa) were probed with five different derivatives of chlorin e6 (CE6) bearing either one of the amino acids serine, lysine, tyrosine or arginine, or monoamino-hexanoic acid as substituent. All derivatives of CE6 (xCE) formed aggregates of a similar structure in aqueous buffer in the millimolar range. In the presence of PVP monomerization of all xCE aggregates could be proved by 1H NMR spectroscopy. xCE-PVP complex formation was confirmed by 1H NMR T2 relaxation and diffusion ordered spectroscopy (DOSY). 1H1H-NOESY data suggested that the xCE uptake into the PVP polymer matrix is governed by hydrophobic interactions. UV–vis absorption and fluorescence emission bands of xCE in the micromolar range revealed characteristic PVP-induced bathochromic shifts. The presented data point out the potential of PVP as carrier system for amphiphilic derivatives of chlorin e6. The capacity of PVP to monomerize xCE aggregates may enhance their efficiency as possible photosensitizers in PDT.

Denkschrift gerichtet an Eine hohe National-Versammlung über die Gesetzes-Vorlagen No. 77, wegen Einführung und Erhöhung der Grundsteuer und über No. 57, wegen Erhöhung der Rübenzucker- und Maischsteuer

Boberach: Eine allgemeine Einkommen- und Vermögenssteuer soll anstelle der von der Regierung beabsichtigten Erhöhung der Grundsteuer aufgrund neuer Kataster und Abschaffung von Grundsteuerbefreiungen eingeführt, das gesamte Steuersystem gründlich überprüft werden. Die Fabrikation von Alkohol aus Kartoffeln darf nicht mit der Maischsteuer gefährdet werden. In der Anlage: Durchschnittliche Tagesbetriebskosten einer größeren preußischen Branntweinbrennerei

Identification of two Th1 cell epitopes on the Babesia bovis-encoded 77-kilodalton merozoite protein (Bb-1) by use of truncated recombinant fusion proteins

Previous studies have demonstrated the serologic and T-cell immunogenicity for cattle of a recombinant form of the apical complex-associated 77-kDa merozite protein of Babesia bovis, designated Bb-1. The present study characterizes the immunogenic epitopes of the Bb-1 protein. A series of recombinant truncated fusion proteins spanning the majority of the Bb-1 protein were expressed in Escherichia coli, and their reactivities with bovine peripheral blood mononuclear cells and T-cell clones derived from B. bovis-immune cattle and with rabbit antibodies were determined. Lymphocytes from two immune cattle were preferentially stimulated by the N-terminal half of the Bb-1 protein (amino acids 23 to 266, termed Bb-1A), localizing the T-cell epitopes to the Bb-1A portion of the molecule. CD4+ T-cell clones derived by stimulation with the intact Bb-1 fusion protein were used to identify two T-cell epitopes in the Bb-1A protein, consisting of amino acids SVVLLSAFSGN VWANEAEVSQVVK and FSDVDKTKSTEKT (residues 23 to 46 and 82 to 94). In contrast, rabbit antiserum raised against the intact fusion protein reacted only with the C-terminal half of the protein (amino acids 267 to 499, termed Bb-1B), which contained 28 tandem repeats of the tetrapeptide PAEK or PAET. Biological assays and Northern (RNA) blot analyses for cytokines revealed that following activation with concanavalin A, T-cell clones reactive against the two Bb-1A epitopes produced interleukin-2, gamma interferon, and tumor necrosis factors beta and alpha, but not interleukin-4, suggesting that the Bb-1 antigen preferentially stimulates the Th1 subset of CD4+ T cells in cattle. The studies described here report for the first time the characterization, by cytokine production, of the Th1 subset of bovine T cells and show that, as in mice, protozoal antigens can induce Th1 cells in ruminants. This first demonstration of B. bovis-encoded Th1 cell epitopes provides a rationale for incorporation of all or part of the Bb-1 protein into a recombinant vaccine.

Die Rechtsstellung der Reichspensionäre : (Artikel 33 (5), 3, 14, 19, 123 (1), 131, 134 Grundgesetz. § 77 des Gesetzes zu Art. 131 GG vom 11.5.51)

George Tzschirner

Na 50 Nachlass Arthur Schopenhauer - 'Schopenhauer-Archiv', 77 - Antwort auf die Briefe vom 18. Juni und 15. Juli 1800

u.a.: Deutschlandreise; Empfehlung eines Theaterstücks von Molière;

Na 1 Nachlass Max Horkheimer, 77 - Korrespondenzen u.a. mit Siegfried Kander (p. II 9, 223-402)

31 Briefe zwischen Siegfried Kander und Max Horkheimer, 1941-1949; 2 Briefe zwischen Harold H. Shaper und Max Horkheimer, 1945 siehe auch Siegfried Kander; 2 Briefe und Beilagen zwischen H. M. Kallen und Max Horkheimer, 1944; 17 Briefe und Beilage zwischen Erika Kickton und Max Horkheimer, 1946-1949; 9 Briefe zwischen Christa Kerti und Max Horkheimer, 1948-1949; 25 Briefe zwischen Else Klee-Hausmann und Max Horkheimer, 1947-1949; 18 Briefe und Beilage zwischen Philip Klein von The New York School of Social Work, American Jewish Committee und Max Horkheimer, 1944-1947; 2 Briefe zwischen Otto Klineberg und Max Horkheimer, 1949;

Ms. Praed. 77 - Postilla cum sermonibus dominicalibus

Vorbesitzer: Dominikanerkloster Frankfurt am Main;